Lu/BCAM Adhesion Glycoprotein Is a Receptor for Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1) (original) (raw)
Figure 6
CNF1 binds to the cell surface via a C-terminal peptide.
Suspensions of HeLa cells (1×105 cells in 1 ml medium) were incubated for 20 min at 4°C with 2 µg DyLight488-labeled GST-CNF1 (CNF1DL488) alone, or together with increasing amounts (up to 30 fold) of untagged non-labeled CNF1 and the non-labeled fragments GST-CNF1-(426–1014), GST-CNF1-(720–1014), GST-CNF1-(709–1014) and GST-CNF1-(1–343), respectively. Following washing with PBS, cells were subjected to FACS analysis. Results are presented as mean of cell surface-bound fluorescence (% of control) of three independent experiments+standard deviation.