Lu/BCAM Adhesion Glycoprotein Is a Receptor for Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1) (original) (raw)
Figure 7
Summary of the CNF1 domain structure.
CNF1 consists of 1014 amino acids. We found amino acids 1–342 responsible for interaction with the laminin receptor precursor (37LRP). Binding to Lu/BCAM occurs with amino acids 720 to 1014. Data from the literature suggest amino acids 683–730 to mediate binding to mammalian cells [16] indicating this part to be responsible for BCAM binding (white box, RBD). The C-domain represents the catalytic part of the toxin. The two hydrophobic helices (H1 and H2) are crucial for insertion into the endosomal membrane.