Transmission-Blocking Antibodies against Mosquito C-Type Lectins for Dengue Prevention (original) (raw)
Figure 5
Feeding the combination of 9 mosGCTLs antisera blocked DENV-2 uptake by a membrane blood feeding.
(A) The interaction of the susceptible mosGCTLs and DENV-2 E protein. The mosGCTL genes exhibiting susceptible role in DENV-2 infection (p<0.05) were cloned into pMT/Bip/V5-His A DNA vector. The recombinant mosGCTLs with a V5 tag in C-terminus were expressed in Drosophila S2 cells. mosGCTL-26 (AAEL017265) was failed to be expressed in Drosophila S2 cells. The interaction was determined by ELISA. An anti-V5 mouse mAb was used to probe the binding activity. The experiment was reproduced twice. (B–D) Feeding the combination of 9 mosGCTL antisera blocked DENV-2 infection. The pre-immune sera, mosGCTL-3 antisera and combined mosGCTLs antisera (1/9 mosGCTL antiserum each) were serially diluted 100- (B), 1,000- (C), or 5,000- (D) fold with fresh human blood and Vero cells-generated DENV-2. The fed mosquitoes were sacrificed on 8 days post infection to determine the infectivity by qPCR. The number of infected mosquitoes/total mosquitoes was presented on the top of each column (Left panel of each subfigure). The mosquito infective ratio (Right panel) was then interpreted from the left panel. One dot represented a mosquito. The result was pooled from 4 independent experiments.