Broad-Spectrum Anti-biofilm Peptide That Targets a Cellular Stress Response (original) (raw)
Figure 1
Peptide 1018 potently inhibited bacterial biofilms at concentrations that did not affect planktonic cell growth.
Sub-inhibitory concentrations of peptide 1018 prevented biofilm development and eradicated or reduced existing biofilms of Gram-negative and Gram-positive bacteria. Concentrations of peptide 1018 used were 10 µg/ml for Pseudomonas aeruginosa (labelled as strains PA14 and PAO1) Escherichia coli 0157, Acinetobacter baumannii and Burkholderia cenocepacia, 20 µg/ml for Salmonella enterica serovar Typhimurium 14028S experiments, 2 µg/ml for Klebsiella pneumoniae experiments, and 2.5 µg/ml for methicillin resistant Staphylococcus aureus (MRSA) experiments. Inhibition of biofilm development was tested by immediately adding 1018 into the flow-through medium of the flow cell apparatus and then monitoring biofilm formation for 3 days. Eradication conditions involved waiting two days before addition of 1018 into the flow-through medium. After 3 days, bacteria were stained green with the all bacteria stain Syto-9 and red with the dead-bacteria stain propidium iodide (merge shows as yellow to red) prior to confocal imaging. Each panel shows reconstructions from the top in the large panel and sides in the right and bottom panels (xy, yz and xz dimensions).