Broad-Spectrum Anti-biofilm Peptide That Targets a Cellular Stress Response (original) (raw)
Figure 4
Genetic complementation of (p)ppGpp synthetase enzymes restored the ability to form biofilms.
The biofilm deficiency of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus HG001 (p)ppGpp mutants (Δ_relAspoT_ and rshSyn respectively) was rescued by genetic complementation [Δ_relAspoT_ + relAspoT+ (+SR) as described [12] and rsh+ [30], respectively] leading to biofilm formation equivalent to WT shown in the left-most panels. After 3 days, bacteria were stained green with the all bacteria stain Syto-9 and red with the dead-bacteria stain propidium iodide (merge shows as yellow to red) prior to confocal imaging. Each panel shows reconstructions from the top in the large panel and sides in the right and bottom panels (xy, yz and xz dimensions).