Transgenic Expression of the Dicotyledonous Pattern Recognition Receptor EFR in Rice Leads to Ligand-Dependent Activation of Defense Responses (original) (raw)
Fig 2
The perception of elf18_E_.coli in EFR and EFR::XA21 rice plants activates several MAP kinases and ROS production.
Fully mature leaves of Ubi::EFR::_GFP_-9-11-12 (A), Ubi::EFR::XA21::_GFP-3-6-7 (B) and Kitaake (C), lines were treated with 1 μM elf18 E.coli for the indicated time. Upper panel anti-p42/44 MAP kinase western blot on total protein extracts, lower panel CBB stain of membrane as loading control. (D) Elf18_E._coli_-triggered ROS production in fully mature leaves of Ubi::EFR::_GFP_-9-11-12, Ubi::EFR::XA21::_GFP-3-6-7 but not Kitaake. Leaves of the indicated genotypes were treated with water (mock) or elf18_E.coli at a final concentration of 100 nM. Data points depict average relative light production ± SE of at least six biological replicates. These experiment were repeated at least three times with similar results.