Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine (original) (raw)
Fig 12
Viral growth of SARS-CoV-∆3 and virulence after serial passage in mice.
(A) Subconfluent monolayers of Vero E6 and DBT-mACE2 cells were infected with wt, ΔE and Δ3 viruses at a moi of 0.001. Culture supernatants collected at 4, 24, 48 and 72 hpi were titrated by plaque assay. (B) 16-week-old BALB/c mice were intranasally inoculated with 100,000 pfu of wt, ΔE and Δ3 viruses. Viral titer in lungs was determined at 2 and 4 days post infection (n = 3, each day). Error bars represent standard deviations. (C) Weight loss and survival were monitored for 10 days (5 mice per group).