Functional Characterization of a Novel Class of Morantel-Sensitive Acetylcholine Receptors in Nematodes (original) (raw)
Fig 4
In-situ hybridizations of Hco-acr-26 and Hco-acr-27 mRNAs in H. contortus.
XL3 larvae of H. contortus were fixed and hybridized with digoxygenin labeled antisense cDNA probes to monitor the localization of Hco-acr-26 (panel A) and Hco-acr-27 mRNAs (panel B) within the worm. Sense probes were used as negative control. cDNA/mRNA hybridomes were detected using primary anti-digoxygenin antibodies in combination with secondary Alexa 594-labeled antibodies (red). Body wall muscular cells were stained using primary antibodies raised against the myosin protein and further revealed with secondary Alexa 488-labeled antibodies (green). The scale bars correspond to 20 μm.