Global Analysis of the Fungal Microbiome in Cystic Fibrosis Patients Reveals Loss of Function of the Transcriptional Repressor Nrg1 as a Mechanism of Pathogen Adaptation (original) (raw)
Fig 2
Diversity of species and antifungal resistance profiles of 1,603 fungal isolates from cystic fibrosis patients.
Each dot represents an isolate from the corresponding cystic fibrosis patient and colours represent species identity. The vertical dispersion of isolates within each phenotypic class is simply for visual clarity, as with the horizontal dispersion of isolates from an individual patient. (A) Summary of yeast isolates. The isolates are categorized as: “Fluconazole resistant” if their relative growth with fixed concentration of fluconazole at 128 μg/ml was greater than 2 times that of the relative growth of reference C. albicans strain SN95; “Fluconazole susceptible” if their relative growth was less than 2 times the relative growth of SN95; and “Variable” if their resistance profiles were variable over biological and technical duplicates. C. albicans is divided into two groups: isolates that show standard yeast morphology in rich medium at 30°C are in blue; and isolates that show filamentous growth under these conditions are in red. (B) Summary of mold isolates. The isolates are categorized as: “Itraconazole resistant” if their relative growth with fixed concentration of itraconazole at 0.5 μg/ml was greater than 2 times that of the relative growth of reference A. fumigatus strain AF293; and “Itraconazole susceptible” if their relative growth was less than 2 times the relative growth of AF293