The Hepatitis C Virus-Induced Membranous Web and Associated Nuclear Transport Machinery Limit Access of Pattern Recognition Receptors to Viral Replication Sites (original) (raw)
Fig 2
Exclusion of RLRs from cytoplasmic regions enriched for HAV proteins.
A and B) Uninfected and HAV-infected Huh7.5 cells were transfected 5 days after viral infection with constructs encoding the catalytically inactive FLAG-tagged RIG-I-K270A. On day 7 after HAV infection, the localization of RIG-I-K270A (green) and either HAV capsid protein (red, panel A) or dsRNA (red, panel B) were evaluated by indirect immunofluorescence confocal microscopy using specific antibodies. DNA was stained with DAPI (blue) and scale bars represent 5 μm. Arrows point to regions of the cytoplasm that contain capsid proteins or dsRNA but lack RIG-I-K270A signal and the boxes in the middle panels represent the area of magnification in the bottom panels.