Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand (original) (raw)
Fig 5
ULBP2-TB40 infected cells have lower MHC class I surface expression than TB40 infected cells.
(A,B) Representative plots showing HLA class I expression using pan-HLA-A,B,C antibody (HLA I) or HLA-A02:01 antibody labeling of infected (IE1/2+) DC. Graphs provide (A) percentage and (B) median fluorescence intensity (MFI) of HLA class I positive uninfected immature DC (imDC) (white circles), or TB40 (black circles) and ULBP2-TB40 (red circles) infected DC of 4 to 6 donors. Horizontal bars represent medians. Data were analyzed with Mann-Whitney t-test. *, P < 0.05. (C) Representative staining for HLA class I on uninfected HFF or HFF infected with 1 PFU/cell of the indicated viruses at day 1 or 2 p.i. The histograms on the right depict ULBP2 expression on TB40, dUL16 TB40 (grey) or ULBP2-TB40 infected cells (red) at the indicated time points p.i. Black line, isotype controls. (D) HLA class I and ULBP2 staining gated on ULBP2-TB40 infected fibroblasts (IE1/2-positive cells) derived from two different donors.