Zika Virus Antagonizes Type I Interferon Responses during Infection of Human Dendritic Cells (original) (raw)
Fig 4
ZIKV infection induces minimal pro-inflammatory cytokine production by DCs.
(A) moDCs were left untreated (“Mock”), transfected with RIG-I agonist (10ng/1e5 cells), or infected with PR-2015, P6-1966, MR-1947, or Dak-1984 at MOI of 1 (n = 7 donors). Supernatants were collected at 48hpi. (B, C) Monocytes (Mo) and myeloid DCs (mDCs) were left untreated (“Mock”), treated with LPS (100 ng/ml), or infected with PR-2015 at MOI of 1 (n = 5 donors). Supernatants were collected at 24hpi. (D) Plasmacytoid DCs (pDCs) were left untreated (“Mock”), treated with R848 (1 μg/ml), or infected with PR-2015 at MOI of 1 (n = 5 donors). Supernatants were collected at 24hpi. Cytokine production was assessed using multiplex bead array. Values for each individual donor are shown with the mean +/- SD. Statistical significance (p< 0.05) was determined using a Kruskal-Wallis test with comparisons made to untreated (“Mock”) cells. See also S2, S3, S4, and S5 Tables.