A VP1 mutation acquired during an enterovirus 71 disseminated infection confers heparan sulfate binding ability and modulates ex vivo tropism (original) (raw)
Fig 4
Replication of EV71-VP1 97R167G , EV71-VP1 97L167E and EV71-VP1 97R/L167G/E in reconstituted airway epithelia developed from the upper (A) and lower (B) respiratory tract and HS/SCARB2 expression in the same tissues (C -D). (A-B) Viral loads were measured by RT-qPCR 5 dpi in the apically collected washes of the apically or basally inoculated tissues and are expressed as the mean ±SEM (N≥2). ***P<0.001, **P<0.01, *P<0.05. Frequency plots are shown on the right (see Fig 3 for details) (C-D) Tissue sections were colored with hematoxylin and eosin (upper panel) or labeled by immunofluorescence. SCARB2 is stained in green, HS in red and cell nuclei in blue. Scale bar = 20 μm.