HEXIM1-Tat chimera inhibits HIV-1 replication (original) (raw)
Fig 4
Stable expression of HT1 inhibits HIV reactivation.
A. Stable expression of HT1 inhibits HIV reactivation from 2D10 cells. 3f:HT1 was stably expressed in 2D10 cells (D-HT cells). Cell lysates were submitted to SDS-PAGE and WB using anti-Flag and anti-GAPDH Abs (lower panel). FACS analysis of GFP positive cells showed that HIV reactivation by PMA, SAHA or JQ1 was significantly impaired in D-HT cells when compared to 2D10 cells, as determined by a student t-test. *** represent differences with p < E-03 (p = 4.75E-05, p = 4.35E-05 and p = 7.62E-05 respectively). Error bars represent standard deviation from triplicate FACS analysis. B. Stable expression of HT1 inhibits HIV reactivation from J-Lat 9.2 cells. 3f:HT1 was stably expressed in J-Lat 9.2 cells (L-HT cells). Cell lysates were submitted to SDS-PAGE and WB using anti-Flag and anti-GAPDH Abs (lower panel). FACS analysis of GFP positive cells showed that HIV reactivation by PMA was significantly impaired in L-HT cells when compared to J-Lat 9.2 cells, as determined by a student t-test. *** represent differences with p < E-03 (p = 5.62E-05). Error bars represent standard deviation from triplicate FACS analysis.