Schistosoma japonicum extracellular vesicle miRNA cargo regulates host macrophage functions facilitating parasitism (original) (raw)
Fig 7
SjEV miR-125b and bantam influence macrophage proliferation.
(A) RAW264.7 cells transfected with SjEV miR-125b and bantam exhibit increased macrophage proliferation. At the indicated time of post treatment, RAW264.7 cells were collected and assayed. Luciferase activities correspond to levels of cell proliferation. (B) Representative results of flow cytometry analysis of cell proliferation in RAW264.7 cells transfected with SjEV miR-125b and bantam mimics. (C) Quantitation of flow cytometry analysis of cell proliferation in RAW264.7 cells transfected with SjEV miR-125b and bantam mimics. The result shows the mean and standard errors from an experiment carried out in triplicate. (D) Injection of SjEVs into mice tail veins leads increases the population of peripheral blood monocytes in mice as determined by flow cytometry analysis. Each experiment shows representative results and illustrates the mean and standard errors derived from 6 mice. (E) The population of peripheral blood monocytes isolated from mice infected with S. japonicum was increased compared to uninfected mice. Each experiment shows representative results and illustrates the mean and standard errors derived from 10–15 mice. (F) TNF-α levels are increased in sera from mice infected with S. japonicum. Each experiment shows representative results and illustrates the mean and standard errors derived from 6 mice. For panel A, C, D, E and F, *P ≤ 0.05 and ** P ≤ 0.01.