The characteristics of the expression of heat shock proteins and cox-2 in the liver of hamsters infected with Clonorchis sinensis, and the change of endocrine hormones and cytokines (original) (raw)

Folia Parasitologica 59[4] 255-263 (2012) | DOI: 10.14411/fp.2012.036

Department of Medical Zoology, Kyung Hee University School of Medicine, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 130-701, Republic of Korea

The liver fluke Clonorchis sinensis (Digenea) is a high-risk parasite that causes serious diseases such as cirrhosis, carcinogenic liver damage and clonorchiasis in East Asia. This study was conducted to evaluate the relationship between stress/endocrine hormones and inflammation induced by infection as well as the expression of heat shock proteins (hsp-27, hsp-90), cox-2 and cytokines in the livers of hamsters infected with C. sinensis. The average body weight of infected hamsters decreased up to 25% compared with that of the control group, and bile duct hyperplasia with inflammation, liver fibrosis and hepatic necrosis were observed in _C. sinensis_-infected livers. The expression of hsp-27, hsp-90, and cox-2 was significantly increased in the livers of _C. sinensis_-infected hamsters compared with the control group. Moreover, the expression levels of inflammatory cytokines (IL-1β, IL-2, TGF-β2 and IFN-α1) were markedly increased in the livers of the infected group compared with those of the control group. Consistently, plasma IL-3 and IL-6 levels gradually increased during the infection period, and the concentration levels of testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), corticosterone, and adrenocorticotropic hormone (ACTH) in _C. sinensis_-infected hamsters increased over 25%, compared with those of the uninfected normal group. These results demonstrate that C. sinensis infection may increase the expression of hsp27, hsp90 and cox-2 as well as it may cause periductal fibrosis, chronic inflammation and hepatic necrosis in the liver. Furthermore, the results indicate that C. sinensis infection induces not only stress-induced hormone imbalance but also the sustained secretion of inflammatory cytokines through chronic stress/stimuli.

Keywords: liver fluke, Digenea, cytokines, cox-2, heat shock proteins, inflammation

Received: January 9, 2012; Accepted: August 14, 2012; Published: December 1, 2012 Show citation

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