Transcriptome Profiling of Human and Murine ESCs Identifies Divergent Paths Required to Maintain the Stem Cell State (original) (raw)

Journal Article

,

Genome Institute of Singapore

,

Singapore

Search for other works by this author on:

,

National Institute of Ageing; Stem Cell

, Laboratory of Neuroscience, Baltimore, Maryland,

USA

Search for other works by this author on:

,

Genome Institute of Singapore

,

Singapore

Department of Biological Sciences, National University of Singapore

,

Singapore

Search for other works by this author on:

,

Genome Institute of Singapore

,

Singapore

Search for other works by this author on:

,

Genome Institute of Singapore

,

Singapore

Search for other works by this author on:

,

Genome Institute of Singapore

,

Singapore

Search for other works by this author on:

,

Genome Institute of Singapore

,

Singapore

Search for other works by this author on:

,

Genome Institute of Singapore

,

Singapore

Search for other works by this author on:

,

National Institute of Ageing; Stem Cell

, Laboratory of Neuroscience, Baltimore, Maryland,

USA

Search for other works by this author on:

,

Embryo Stem Cell International

,

Singapore

Search for other works by this author on:

... Show more

Accepted:

15 September 2004

Published:

01 February 2005

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Chia Lin Wei, Takumi Miura, Paul Robson, Sai‐Kiang Lim, Xiu‐Qin Xu, Mathia Yu‐Chuan Lee, Sanjay Gupta, Lawrence Stanton, Yongquan Luo, Jacqui Schmitt, Scott Thies, Wei Wang, Irina Khrebtukova, Daixing Zhou, Edison T. Liu, Yi Jun Ruan, Mahendra Rao, Bing Lim, Transcriptome Profiling of Human and Murine ESCs Identifies Divergent Paths Required to Maintain the Stem Cell State, Stem Cells, Volume 23, Issue 2, February 2005, Pages 166–185, https://doi.org/10.1634/stemcells.2004-0162
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

Human embryonic stem cells (hESCs) are an important source of stem cells in regenerative medicine, and much remains unknown about their molecular characteristics. To develop a detailed genomic profile of ESC lines in two different species, we compared transcriptomes of one murine and two different hESC lines by massively parallel signature sequencing (MPSS). Over 2 million signature tags from each line and their differentiating embryoid bodies were sequenced. Major differences and conserved similarities between species identified by MPSS were validated by reverse transcription polymerase chain reaction (RT‐PCR) and microarray. The two hESC lines were similar overall, with differences that are attributable to alleles and propagation. Human–mouse comparisons, however, identified only a small (core) set of conserved genes that included genes known to be important in ESC biology, as well as additional novel genes. Identified were major differences in leukemia inhibitory factor, transforming growth factor‐beta, and Wnt and fibroblast growth factor signaling pathways, as well as the expression of genes encoding metabolic, cytoskeletal, and matrix proteins, many of which were verified by RT‐PCR or by comparing them with published databases. The study reported here underscores the importance of cross‐species comparisons and the versatility and sensitivity of MPSS as a powerful complement to current array technology.

Copyright © 2005 AlphaMed Press

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Transcriptome Profiling of Human and Murine ESCs Identifies Divergent Paths Required to Maintain the Stem Cell State - 24 Hours access

EUR €51.00

GBP £44.00

USD $55.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 834

705 Pageviews

129 PDF Downloads

Since 1/1/2022

Citations

159 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic