Transcriptome Profiling of Human and Murine ESCs Identifies Divergent Paths Required to Maintain the Stem Cell State (original) (raw)
Journal Article
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Genome Institute of Singapore
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Singapore
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National Institute of Ageing; Stem Cell
, Laboratory of Neuroscience, Baltimore, Maryland,
USA
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Genome Institute of Singapore
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Singapore
Department of Biological Sciences, National University of Singapore
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Singapore
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Genome Institute of Singapore
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Singapore
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Genome Institute of Singapore
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Singapore
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Genome Institute of Singapore
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Singapore
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Genome Institute of Singapore
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Singapore
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Genome Institute of Singapore
,
Singapore
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National Institute of Ageing; Stem Cell
, Laboratory of Neuroscience, Baltimore, Maryland,
USA
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Embryo Stem Cell International
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Singapore
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Accepted:
15 September 2004
Published:
01 February 2005
Cite
Chia Lin Wei, Takumi Miura, Paul Robson, Sai‐Kiang Lim, Xiu‐Qin Xu, Mathia Yu‐Chuan Lee, Sanjay Gupta, Lawrence Stanton, Yongquan Luo, Jacqui Schmitt, Scott Thies, Wei Wang, Irina Khrebtukova, Daixing Zhou, Edison T. Liu, Yi Jun Ruan, Mahendra Rao, Bing Lim, Transcriptome Profiling of Human and Murine ESCs Identifies Divergent Paths Required to Maintain the Stem Cell State, Stem Cells, Volume 23, Issue 2, February 2005, Pages 166–185, https://doi.org/10.1634/stemcells.2004-0162
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Abstract
Human embryonic stem cells (hESCs) are an important source of stem cells in regenerative medicine, and much remains unknown about their molecular characteristics. To develop a detailed genomic profile of ESC lines in two different species, we compared transcriptomes of one murine and two different hESC lines by massively parallel signature sequencing (MPSS). Over 2 million signature tags from each line and their differentiating embryoid bodies were sequenced. Major differences and conserved similarities between species identified by MPSS were validated by reverse transcription polymerase chain reaction (RT‐PCR) and microarray. The two hESC lines were similar overall, with differences that are attributable to alleles and propagation. Human–mouse comparisons, however, identified only a small (core) set of conserved genes that included genes known to be important in ESC biology, as well as additional novel genes. Identified were major differences in leukemia inhibitory factor, transforming growth factor‐beta, and Wnt and fibroblast growth factor signaling pathways, as well as the expression of genes encoding metabolic, cytoskeletal, and matrix proteins, many of which were verified by RT‐PCR or by comparing them with published databases. The study reported here underscores the importance of cross‐species comparisons and the versatility and sensitivity of MPSS as a powerful complement to current array technology.
Copyright © 2005 AlphaMed Press
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