An Autogeneic Feeder Cell System That Efficiently Supports Growth of Undifferentiated Human Embryonic Stem Cells (original) (raw)
Journal Article
Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
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Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
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Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
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School of Biological and Biomedical Sciences, University of Durham
, Durham,
UK
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Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
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Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
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Newcastle Fertility Centre at Life, Newcastle Health Service
, Newcastle upon Tyne,
UK
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Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
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Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle
, Newcastle upon Tyne,
UK
Correspondence: M. Stojkovic, Ph.D.Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Central Parkway, Newcastle upon Tyne, NE1 3BZ, UK. Telephone: 44‐191‐241‐8638; Fax: 44‐191‐219‐4747;e‐mail: [email protected]
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Accepted:
01 September 2004
Cite
Petra Stojkovic, Majlinda Lako, Rebecca Stewart, Stefan Przyborski, Lyle Armstrong, Jerome Evans, Alison Murdoch, Tom Strachan, Miodrag Stojkovic, An Autogeneic Feeder Cell System That Efficiently Supports Growth of Undifferentiated Human Embryonic Stem Cells, Stem Cells, Volume 23, Issue 3, March 2005, Pages 306–314, https://doi.org/10.1634/stemcells.2004-0137
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Abstract
Human embryonic stem cells (hESCs) have great potential as a source of cells for therapeutic uses, but their culture requires the support of mouse or human cells, either directly as a feeder cell layer or indirectly as a source of conditioned medium in feeder‐free culture systems. Unfortunately, the risks of cross‐transfer of pathogens from xenogeneic or allogeneic feeders or cell by‐products limit their medical applications. In addition, not all human feeders support the growth of hESCs equally well, and ethical concerns have been raised regarding the derivation of feeder cells from aborted human fetuses.
We report here the culture of hESCs on a novel feeder cell system, comprising fibroblast‐like cells derived from the spontaneous differentiation of hESCs. Isogenicity of the hESCs and hESC‐derived fibroblasts was confirmed by micro satellite analysis. The nature of the hESC‐derived fibroblasts was identified by the expression of specific markers. This feeder system permits continuous growth of undifferentiated and pluripotent hESCs, as demonstrated by the expression of specific hESC markers, by the formation of teratomas after injection of hESCs into severely combined immunodeficient mice, and by in vitro differentiation of hESCs into differentiated cells of ectodermal, endodermal, and mesodermal origin. Feeder cells derived from hESCs offers a potentially more secure autogeneic and genotypically homogenous system for the growth of undifferentiated hESCs.
Copyright © 2005 AlphaMed Press
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