Early Alterations in Cytokine Expression in Adult Compared to Developing Lung in Mice after Radiation Exposure (original) (raw)
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10 February 2010 Early Alterations in Cytokine Expression in Adult Compared to Developing Lung in Mice after Radiation Exposure
Carl J. Johnston,Eric Hernady,Christina Reed,Sally W. Thurston,Jacob N. Finkelstein,Jacqueline P. Williams
Author Affiliations +
Carl J. Johnston,1,2 Eric Hernady,3 Christina Reed,1 Sally W. Thurston,4 Jacob N. Finkelstein,1,2,3 Jacqueline P. Williams3
1aDepartments of Pediatrics and Neonatology, University of Rochester Medical Center, Rochester, New Y
2bDepartments of Environmental Medicine, University of Rochester Medical Center, Rochester, New York
3cDepartments of Radiation Oncology and, University of Rochester Medical Center, Rochester, New York
4dDepartments of Biostatistics and Computational Biology, University of Rochester Medical Center, Roc
Abstract
To assess early changes in the lung after low-dose radiation exposure that may serve as targets for mitigation of lung injury in the aftermath of a terrorist event, we analyzed cytokine expression after irradiation. Adult mice were studied after whole-lung or total-body irradiation. Mouse pups of different ages were also investigated after total-body irradiation. mRNA abundance was analyzed in tissue and plasma, and pathological changes were assessed. In lung tissue, dose-related changes were seen in IL1B, IL1R2 and CXCR2 mRNA expression at 1 and 6 h after irradiation, concurrent with increases in plasma protein levels of KC/CXCL1 and IL6. However, in the pups, changes in IL1 abundance were not detected until 28 days of age, coincident with the end of postnatal lung growth, although apoptosis was detected at all ages. In conclusion, although cytokines were expressed after low doses of radiation, their role in the progression of tissue response is yet to be determined. They may be candidates for use in marker-based biodosimetry. However, the lack of cytokine induction in early life suggests that different end points (and mitigating treatments) may be required for children.
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Carl J. Johnston, Eric Hernady, Christina Reed, Sally W. Thurston, Jacob N. Finkelstein, and Jacqueline P. Williams "Early Alterations in Cytokine Expression in Adult Compared to Developing Lung in Mice after Radiation Exposure," Radiation Research 173(4), 522-535, (10 February 2010). https://doi.org/10.1667/RR1882.1
Received: 3 June 2009; Accepted: 1 September 2009; Published: 10 February 2010
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