Effects of Low-Dose Radiation on the Immune System of Mice after Total-Body Irradiation (original) (raw)
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23 July 2010 Effects of Low-Dose Radiation on the Immune System of Mice after Total-Body Irradiation
Enikő Noémi Bogdándi,Andrea Balogh,Nikolett Felgyinszki,Tünde Szatmári,Eszter Persa,Guido Hildebrandt,Géza Sáfrány,Katalin Lumniczky
Author Affiliations +
Enikő Noémi Bogdándi,1 Andrea Balogh,2 Nikolett Felgyinszki,2 Tünde Szatmári,2 Eszter Persa,2 Guido Hildebrandt,3 Géza Sáfrány,1 Katalin Lumniczky2,*
1aDepartment of Molecular and Tumor Radiobiology, Frédéric Joliot-Curie National Research Institute f
2bDepartment of Cellular and Immune Radiobiology, Frédéric Joliot-Curie National Research Institute f
3cDepartment of Radiotherapy, University of Rostock, Medical Faculty, Rostock, Germany
*Address for correspondence to: Department of Cellular and Immune Radiobiology, Frédéric Joliot-Curie National Research Institute for Radiobiology and Radiohygiene, 1221 Budapest, Anna u. 5, Hungary; e-mail: lumniczky.katalin@osski.hu.
Abstract
The effects of acute exposure to low- and high-dose radiation on the quantitative and functional parameters of the immune system were analyzed. C57BL/6 mice were irradiated with different doses of γ radiation (0.01, 0.05, 0.1, 0.5 and 2 Gy) and splenocytes were isolated at various times. Alterations in the distribution and surviving fraction of splenocyte subsets such as CD4 and CD8 T lymphocytes, regulatory T cells (Treg), natural killer (NK) cells, dendritic cells (DCs) and B lymphocytes were analyzed by flow cytometry. Apoptosis frequency was quantified by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method 4 h after irradiation. Cytokine expression was investigated by real-time reverse transcription-polymerase chain reaction (RT-PCR). Low doses decreased apoptosis in the splenocyte subpopulations studied most prominently in NK cells and DCs. Exposure to 2 Gy increased apoptosis in all splenocyte subpopulations; B cells were the most sensitive and NK cells and DCs the least sensitive. The lowest cell numbers were measured 3 days after irradiation, with minor changes by day 7. CD8 and B cells were rather resistant to low doses but were very sensitive to 2 Gy, while NK cells, DCs and Treg cells were much more resistant to high doses. Expression of the T-helper 1 (Th1)- and helper 2 (Th2)-type cytokines decreased after low doses and increased after high doses. Interleukin 6 (IL-6) reacted at early times and IL-10 at later times. IL-5 levels were consistently elevated. These data highlight the differences in the responses of different splenocyte subpopulations to low- and high-dose radiation.
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Enikő Noémi Bogdándi, Andrea Balogh, Nikolett Felgyinszki, Tünde Szatmári, Eszter Persa, Guido Hildebrandt, Géza Sáfrány, and Katalin Lumniczky "Effects of Low-Dose Radiation on the Immune System of Mice after Total-Body Irradiation," Radiation Research 174(4), 480-489, (23 July 2010). https://doi.org/10.1667/RR2160.1
Received: 29 January 2010; Accepted: 1 May 2010; Published: 23 July 2010
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