Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice (original) (raw)
Metabolism| October 13 2014
1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
2Mouse Metabolic Phenotyping Center, Vanderbilt University, Nashville, TN
3Division of Cardiovascular and Diabetes Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, U.K.
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4Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University, Nashville, TN
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
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4Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University, Nashville, TN
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5East Carolina Diabetes and Obesity Institute and Departments of Physiology and Kinesiology, East Carolina University, Greenville, NC
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5East Carolina Diabetes and Obesity Institute and Departments of Physiology and Kinesiology, East Carolina University, Greenville, NC
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5East Carolina Diabetes and Obesity Institute and Departments of Physiology and Kinesiology, East Carolina University, Greenville, NC
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5East Carolina Diabetes and Obesity Institute and Departments of Physiology and Kinesiology, East Carolina University, Greenville, NC
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
2Mouse Metabolic Phenotyping Center, Vanderbilt University, Nashville, TN
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1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN
4Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University, Nashville, TN
6Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN
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Diabetes 2014;63(11):3699–3710
Citation
Li Kang, Chunhua Dai, Mary E. Lustig, Jeffrey S. Bonner, Wesley H. Mayes, Shilpa Mokshagundam, Freyja D. James, Courtney S. Thompson, Chien-Te Lin, Christopher G.R. Perry, Ethan J. Anderson, P. Darrell Neufer, David H. Wasserman, Alvin C. Powers; Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice. _Diabetes 1 November 2014; 63 (11): 3699–3710. https://doi.org/10.2337/db13-1845
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Elevated reactive oxygen species (ROS) are linked to insulin resistance and islet dysfunction. Manganese superoxide dismutase (SOD2) is a primary defense against mitochondrial oxidative stress. To test the hypothesis that heterozygous SOD2 deletion impairs glucose-stimulated insulin secretion (GSIS) and insulin action, wild-type (sod2+/+) and heterozygous knockout mice (sod2+/−) were fed a chow or high-fat (HF) diet, which accelerates ROS production. Hyperglycemic (HG) and hyperinsulinemic-euglycemic (HI) clamps were performed to assess GSIS and insulin action in vivo. GSIS during HG clamps was equal in chow-fed sod2+/− and sod2+/+ but was markedly decreased in HF-fed sod2+/−. Remarkably, this impairment was not paralleled by reduced HG glucose infusion rate (GIR). Decreased GSIS in HF-fed sod2+/− was associated with increased ROS, such as superoxide ion. Surprisingly, insulin action determined by HI clamps did not differ between sod2+/− and sod2+/+ of either diet. Since insulin action was unaffected, we hypothesized that the unchanged HG GIR in HF-fed sod2+/− was due to increased glucose effectiveness. Increased GLUT-1, hexokinase II, and phospho-AMPK protein in muscle of HF-fed sod2+/− support this hypothesis. We conclude that heterozygous SOD2 deletion in mice, a model that mimics SOD2 changes observed in diabetic humans, impairs GSIS in HF-fed mice without affecting insulin action.
© 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
2014
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