Butyrate Regulates Liver Mitochondrial Function, Efficiency, and Dynamics in Insulin-Resistant Obese Mice (original) (raw)

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Pharmacology and Therapeutics| February 21 2017

Maria Pina Mollica;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Giuseppina Mattace Raso;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Gina Cavaliere;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Giovanna Trinchese;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Chiara De Filippo;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Serena Aceto;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Marina Prisco;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Claudio Pirozzi;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Francesca Di Guida;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Adriano Lama;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Marianna Crispino;

1Department of Biology, University of Naples Federico II, Naples, Italy

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Diana Tronino;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Paola Di Vaio;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Roberto Berni Canani;

3Department of Translational Medical Science, University of Naples Federico II, Naples, Italy

4European Laboratory for Investigation of Food Induced Diseases, University of Naples Federico II, Naples, Italy

5CEINGE Advanced Biotechnology, University of Naples Federico II, Naples, Italy

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Antonio Calignano;

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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Rosaria Meli

2Department of Pharmacy, University of Naples Federico II, Naples, Italy

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1

M.P.M. and G.M.R. contributed equally to this work.

Diabetes 2017;66(5):1405–1418

Citation

Maria Pina Mollica, Giuseppina Mattace Raso, Gina Cavaliere, Giovanna Trinchese, Chiara De Filippo, Serena Aceto, Marina Prisco, Claudio Pirozzi, Francesca Di Guida, Adriano Lama, Marianna Crispino, Diana Tronino, Paola Di Vaio, Roberto Berni Canani, Antonio Calignano, Rosaria Meli; Butyrate Regulates Liver Mitochondrial Function, Efficiency, and Dynamics in Insulin-Resistant Obese Mice. _Diabetes 1 May 2017; 66 (5): 1405–1418. https://doi.org/10.2337/db16-0924

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Fatty liver, oxidative stress, and mitochondrial dysfunction are key pathophysiological features of insulin resistance and obesity. Butyrate, produced by fermentation in the large intestine by gut microbiota, and its synthetic derivative, the N-(1-carbamoyl-2-phenyl-ethyl) butyramide, FBA, have been demonstrated to be protective against insulin resistance and fatty liver. Here, hepatic mitochondria were identified as the main target of the beneficial effect of both butyrate-based compounds in reverting insulin resistance and fat accumulation in diet-induced obese mice. In particular, butyrate and FBA improved respiratory capacity and fatty acid oxidation, activated the AMPK–acetyl-CoA carboxylase pathway, and promoted inefficient metabolism, as shown by the increase in proton leak. Both treatments consistently increased utilization of substrates, especially fatty acids, leading to the reduction of intracellular lipid accumulation and oxidative stress. Finally, the shift of the mitochondrial dynamic toward fusion by butyrate and FBA resulted in the improvement not only of mitochondrial cell energy metabolism but also of glucose homeostasis. In conclusion, butyrate and its more palatable synthetic derivative, FBA, modulating mitochondrial function, efficiency, and dynamics, can be considered a new therapeutic strategy to counteract obesity and insulin resistance.

© 2017 by the American Diabetes Association.

2017

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