Bioavailability of orange juice (poly)phenols: β-glucan-rich oat bran decreases urinary excretion of flavanone phase II metabolites and enhances excretion of microbiota-derived phenolic catabolites (original) (raw)

Pereira-Caro, Gema, Almutairi, Tahani M., Cáceres-Jiménez, Salud, Moreno-Rojas, José Manuel, Malkova, Dalia ORCID: https://orcid.org/0000-0002-8085-0528, Garcia, Ada L. ORCID: https://orcid.org/0000-0002-3526-2380 and Crozier, Alan(2023) Bioavailability of orange juice (poly)phenols: β-glucan-rich oat bran decreases urinary excretion of flavanone phase II metabolites and enhances excretion of microbiota-derived phenolic catabolites.Free Radical Biology and Medicine, 199, pp. 34-43. (doi: 10.1016/j.freeradbiomed.2023.02.002) (PMID:36764628)

Abstract

The impact of β-glucan-rich oat bran on the bioavailability of orange juice (OJ) flavanones was investigated. Volunteers consumed 500 mL of OJ with and without 22 g of oat bran containing 6 g of β-glucan (OB-6). Urine collected 12 h prior to and over a 0–24 h period post-supplementation was analysed by UHPLC-HRMS. Sixteen flavanone metabolites and thirty-nine colon-derived phenolic catabolites were identified and quantified. The major compounds were hesperetin-3′-glucuronide, along with hippuric acids and the C6–C3 phenolic acids 3-(3′-hydroxy-4′-methoxyphenyl)hydracrylic acid and 3-(4′-hydroxy-3′-methoxyphenyl)propanoic acid. A marked reduction in the 0–24 h excretion of flavanone metabolites from 29.7 μmol (9.3% recovery) to 9.3 μmol (2.9% recovery), occurred following consumption of OB-6 compared to OJ. This appeared not to be an effect of fiber on the rate of transport in the upper gut. After consumption of OJ there was a 163 ± 15 μmol excretion of colon-derived phenolic catabolites, equivalent to 43% of (poly)phenol intake and following OB-6 intake there was a further significant 30% increase. The β-oat bran in OB-6 contained 5.8 μmol of free and 52 μmol of bound phenolic derivatives compared to 371 μmol of OJ (poly)phenols. The elevated excretion of phenolics after OB-6 consumption appears not to be due to bound phenolics in the bran, rather it is consequence, principally, of a bran-mediated increase in the quantities of flavanones passing from the upper to the lower bowel where they were subjected to microbiota-mediated catabolism.

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