Uduak Sunday Ukekpe | Federal Polytechnic Damaturu (original) (raw)
Papers by Uduak Sunday Ukekpe
Medicinal Chemistry Research, 2004
Bread is a cereal product which provides essential nutrients to the body. Several fungi have been... more Bread is a cereal product which provides essential nutrients to the body. Several fungi have been associated with bread spoilage yet bread fungi may offer some benefits. The studied aimed to assess the phytochemicals present in bread fungi and the antimicrobial effects of the fungal isolates. The fungi were isolated using the pour plate technique with Potato dextrose agar and Tryptone soya agar as the growth medium. The fungi were characterized and identified based on their morphological characteristics. The fungi, Aspergillus spp, Rhizopus spp, Fuasrium spp, Mucor spp, Alternaria spp, Tricothecium spp, Sporendonema spp Trichothecium spp and Clasdosporium spp. occurred most frequently in the bread sample. The fungal isolates contained phytochemicals such as alkaloids, tanins, saponins, phenolics, flavonoids and steroids, which reflects its ability to produce bioactive compounds which could serve both environmental and medicinal purposes. The trends of zone of bacteria inhibition for E,coli was penicillium spp< Aspergillus spp< Fuasrium spp< Rhizopus spp, for staphyloccus was penicillum spp< Fuasrium spp <Aspergillus spp etc.
Fish is a high quality food that is rich in protein, fat, vitamin, water and minerals (Lands, 200... more Fish is a high quality food that is rich in protein, fat, vitamin, water and minerals (Lands, 2002). Fish also contains 2 4% lipids which comprises of high level of polyunsaturated fatty acid that are prone to oxidation, resulting in off-flavour, changes in colour, taste and texture or even loss of nutrient (Saeed and Howell, 2002). It is an extremely perishable food with most species becoming inedible within twelve hours at tropical temperatures and spoilage begins as soon as the fish dies (George, 2006). The biggest problem with deterioration in the quality of fish oil is rancidity and the main cause of fish rancidity is oxidation.
4 Abstract: Onion is one of the oldest cultivated bulb producing plant species and second most va... more 4 Abstract: Onion is one of the oldest cultivated bulb producing plant species and second most valuable vegetable after tomatoes in the world but is subject to great production loss due to its poor storability. Refractometric analysis was carried on some randomly selected production stock of onion bulbs in Bade district of Yobe state, Nigeria. This is because, soluble solid constitute most of the content of the onion bulb and dehydrator onion cultivars are developed from lines with high dry matter contents. Although dehydration is considered as one of the oldest and important method of onion processing for storage, it is yet to be utilised in this part of the world. The results of the study showed that different cultivars have different amount of soluble solid determined by their refractive index values which varies considerably and that smaller and medium size bulbs have more dry matter content than larger bulbs. The local white and red onion bulb cultivars have the potentials for ...
Journal of Chemical Society of Nigeria, 2019
Khaya senegalensis, a member of the family maliaceae, is a plant commonly used by the local peopl... more Khaya senegalensis, a member of the family maliaceae, is a plant commonly used by the local people of Nigeria for the treatment of dysentery, mucous, diarrhoea, vermifuge, taeniacide, depurative, Syphilis jaundice, dermatoses, scorpion bites, allergies infection of the gum, hookworm, and disinfection of the wounds. This work evaluates the phytochemical constituents of the plant's stem bark and the antioxidant properties of acetone, ethanol and methanol extracts of the plant part. The stem bark extracts of the plant were obtained by cold sequential extraction using the solvents, acetone, ethanol and methanol in that order. The three solvents show ability to extract some components of the plant's part, though and varied quantities. The result for the phytochemical screening shows that saponin, tannin, alkaloid, cardiac glycoside, flavonoids, phlobatannin some of the phytochemicals of the plant's part and could be responsible for its medicinal properties. The investigation of antioxidants properties of the extracts show acetone, ethanol and methanol to exhibit 66.282%, 84.838%, 85.921% scavenging ability respectively at 100µg/cm 3 compared with 97.437% of the standard, ascorbic acid. The extract therefore can be said to have good antioxidant property and hence can be applied in managing oxidative stress.
International Journal of Advanced Research, May 2013
Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used p... more Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used plant gum especially in food and pharmaceutical industries due to its non toxicity and excellent properties as emulsifiers and stabilizers in emulsions. It consists of complex polysaccharides and glycoprotein, and its qualities are defined by the biophysical properties of the gum. Analyses of some biophysical properties using range of techniques were carried out for harvested, untreated gum Arabic from Sudan-savannah ecological zone of Nigeria. The results revealed that it has some qualities which were in agreement with the international standard specified for gum Arabic. The soil type of the study area was found to be clayey-sandy in nature.
The root bark of Massularia acuminata has been used for medicinal purpose in Akwa Ibom State, Nig... more The root bark of Massularia acuminata has been used for medicinal purpose in Akwa Ibom State, Nigeria. The plant part has been established to be rich in some phytochemicals. This work evaluates the free radical scavenging ability and hence, antioxidant property of ethyl acetate, acetone, ethanol and methanol extracts of the plant part by the DPPH free radical scavenging method. Ethyl acetate extract exhibited the least free radical scavenging ability of 49.71±0.17 % at 100μg/ml concentration. But methanol, ethanol and acetone extracts showed appreciable scavenging ability of 67.47±0.30, 65.01±0.15 and 63.64±0.17 % respectively, values which are comparable with that of the common antioxidant standard, ascorbic acid. Therefore the three extracts can be said to possess reasonable antioxidant property. The variation in the free radical scavenging ability of the extracts shows that extracting solvents play a role in the types of phytochemicals extracted and hence antioxidant property. Based on the outcome of this work, the plant part can be said to possess antioxidant phytochemicals which can be used to manage oxidative stress.
Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used p... more Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used plant gum especially in food and pharmaceutical industries due to its non toxicity and excellent properties as emulsifiers and stabilizers in emulsions. It consists of complex polysaccharides and glycoprotein, and its qualities are defined by the biophysical properties of the gum. Analyses of some biophysical properties using range of techniques were carried out for harvested, untreated gum Arabic from Sudan-savannah ecological zone of Nigeria. The results revealed that it has some qualities which were in agreement with the international standard specified for gum Arabic. The soil type of the study area was found to be clayey-sandy in nature.
ABSTRACT A triterpenoid saponin was isolated from the methanol extract of the root bark of Massul... more ABSTRACT
A triterpenoid saponin was isolated from the methanol extract of the root bark of Massularia acuminata spp. The
compound was identified as 3-O – β- D- glucopyranosyl – (1-4)- β- D- glucopyranosyl – (1-4)- β- Dglucopyranosyl-
olean -12-en-28-oic acid on the basis of chemical and spectral evidence of IR and NMR (1H, 13C,
DEPT, 1H-1H COSY, HSQC and HMBC) data. The compound showed good inhibitory activity against the
microorganisms, Salmonella spp, Staphylococcus aureus, Escherichia coli, Aspergillus niger and Candida albicans
indicating that it is a bioactive compound. It was also found to possess antioxidant property by virtue of its high
DPPH free radical scavenging ability of 73.09% at 100μg/ml, property that can make it relevant in stress
management and cosmetic industry.
KEYWORDS: Massularia acuminata, Triterpenoid saponins, Antimicrobial, Antioxidant, Isolation, Glycoside,
Spectroscopy.
Onion is one of the oldest cultivated bulb producing plant species and second most valuable veget... more Onion is one of the oldest cultivated bulb producing plant species and second most valuable vegetable after tomatoes in the world but is subject to great production loss due to its poor storability. Refractometric analysis was carried on some randomly selected production stock of onion bulbs in Bade district of Yobe state, Nigeria. This is because, soluble solid constitute most of the content of the onion bulb and dehydrator onion cultivars are developed from lines with high dry matter contents. Although dehydration is considered as one of the oldest and important method of onion processing for storage, it is yet to be utilised in this part of the world. The results of the study showed that different cultivars have different amount of soluble solid determined by their refractive index values which varies considerably and that smaller and medium size bulbs have more dry matter content than larger bulbs. The local white and red onion bulb cultivars have the potentials for dehydration compare to the other cultivars within the location of the study area.
The root bark extracts of Massularia acuminata from Akwa Ibom State, Nigeria, (locally known as "... more The root bark extracts of Massularia acuminata from Akwa Ibom State, Nigeria, (locally known as " okok edi ") have been investigated for its phytochemical constituents and antimicrobial activities. Air dried pulverized root bark of the plant part was sequentially solvent extracted with hexane, chloroform, ethyl acetate, acetone, ethanol and methanol. The phytochemical screening of the extracts show the presence of alkaloids, anthraquinones, saponins, phlobatannins, cardiac glycosides, tannins, terpenoids, steroids, flavonoids and resins, but not across all the extracts. Ethyl acetate, acetone, ethanol and methanol extracts were assayed for their antimicrobial activities and all were found to be active against the clinical isolates, Salmonella spp. Staphylococcus aureus, Eschurichia coli, Aspergillus nigger and Candida albicans but at varied level. The presence of some phytochemicals with known pharmacological effects in the plant part extracts and their antimicrobial activities justify the traditional use of the plant part for medicinal purpose.
ABSTRACT Fish contains fats/oils as part of their nutrients which undergo rancidity just as other... more ABSTRACT
Fish contains fats/oils as part of their nutrients which undergo rancidity just as
other oils. The rancidity of oils within fishes denotes fish spoilage. In this work,
time course monitoring of oil rancidity in five fish species; tilapia, mud, cat,
elephant snout and butter fishes harvested from Hadejia-Nguru wetland was carried
out by the thiobarbituric acid (TBA) value method to know their keeping quality. It
was found that, at six hour keeping, elephant snout, Mud and butter fishes were no
longer fresh based on their respective TBA values of 7.40, 7.10 and 6.50
mgMA/kg. The TBA values of 9.50, 11.0 and 8.50 mgMA/kg, respectively for
butter, elephant snout, and mud fishes at twelve hours of keeping indicated that
they have spoiled, while Tilapia and cat fishes with TBA values of 6.60 and
8,00mgMA/kg, respectively at the same twelve hours of keeping show the fishes to
have better keeping quality than the other three species. Generally, preservative
measures are required for cat, tilapia and mud fishes within nine hours of storage
and elephant snout and butter fishes need preservative measures immediately after
harvest for their long keeping.
Medicinal Chemistry Research, 2004
Bread is a cereal product which provides essential nutrients to the body. Several fungi have been... more Bread is a cereal product which provides essential nutrients to the body. Several fungi have been associated with bread spoilage yet bread fungi may offer some benefits. The studied aimed to assess the phytochemicals present in bread fungi and the antimicrobial effects of the fungal isolates. The fungi were isolated using the pour plate technique with Potato dextrose agar and Tryptone soya agar as the growth medium. The fungi were characterized and identified based on their morphological characteristics. The fungi, Aspergillus spp, Rhizopus spp, Fuasrium spp, Mucor spp, Alternaria spp, Tricothecium spp, Sporendonema spp Trichothecium spp and Clasdosporium spp. occurred most frequently in the bread sample. The fungal isolates contained phytochemicals such as alkaloids, tanins, saponins, phenolics, flavonoids and steroids, which reflects its ability to produce bioactive compounds which could serve both environmental and medicinal purposes. The trends of zone of bacteria inhibition for E,coli was penicillium spp< Aspergillus spp< Fuasrium spp< Rhizopus spp, for staphyloccus was penicillum spp< Fuasrium spp <Aspergillus spp etc.
Fish is a high quality food that is rich in protein, fat, vitamin, water and minerals (Lands, 200... more Fish is a high quality food that is rich in protein, fat, vitamin, water and minerals (Lands, 2002). Fish also contains 2 4% lipids which comprises of high level of polyunsaturated fatty acid that are prone to oxidation, resulting in off-flavour, changes in colour, taste and texture or even loss of nutrient (Saeed and Howell, 2002). It is an extremely perishable food with most species becoming inedible within twelve hours at tropical temperatures and spoilage begins as soon as the fish dies (George, 2006). The biggest problem with deterioration in the quality of fish oil is rancidity and the main cause of fish rancidity is oxidation.
4 Abstract: Onion is one of the oldest cultivated bulb producing plant species and second most va... more 4 Abstract: Onion is one of the oldest cultivated bulb producing plant species and second most valuable vegetable after tomatoes in the world but is subject to great production loss due to its poor storability. Refractometric analysis was carried on some randomly selected production stock of onion bulbs in Bade district of Yobe state, Nigeria. This is because, soluble solid constitute most of the content of the onion bulb and dehydrator onion cultivars are developed from lines with high dry matter contents. Although dehydration is considered as one of the oldest and important method of onion processing for storage, it is yet to be utilised in this part of the world. The results of the study showed that different cultivars have different amount of soluble solid determined by their refractive index values which varies considerably and that smaller and medium size bulbs have more dry matter content than larger bulbs. The local white and red onion bulb cultivars have the potentials for ...
Journal of Chemical Society of Nigeria, 2019
Khaya senegalensis, a member of the family maliaceae, is a plant commonly used by the local peopl... more Khaya senegalensis, a member of the family maliaceae, is a plant commonly used by the local people of Nigeria for the treatment of dysentery, mucous, diarrhoea, vermifuge, taeniacide, depurative, Syphilis jaundice, dermatoses, scorpion bites, allergies infection of the gum, hookworm, and disinfection of the wounds. This work evaluates the phytochemical constituents of the plant's stem bark and the antioxidant properties of acetone, ethanol and methanol extracts of the plant part. The stem bark extracts of the plant were obtained by cold sequential extraction using the solvents, acetone, ethanol and methanol in that order. The three solvents show ability to extract some components of the plant's part, though and varied quantities. The result for the phytochemical screening shows that saponin, tannin, alkaloid, cardiac glycoside, flavonoids, phlobatannin some of the phytochemicals of the plant's part and could be responsible for its medicinal properties. The investigation of antioxidants properties of the extracts show acetone, ethanol and methanol to exhibit 66.282%, 84.838%, 85.921% scavenging ability respectively at 100µg/cm 3 compared with 97.437% of the standard, ascorbic acid. The extract therefore can be said to have good antioxidant property and hence can be applied in managing oxidative stress.
International Journal of Advanced Research, May 2013
Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used p... more Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used plant gum especially in food and pharmaceutical industries due to its non toxicity and excellent properties as emulsifiers and stabilizers in emulsions. It consists of complex polysaccharides and glycoprotein, and its qualities are defined by the biophysical properties of the gum. Analyses of some biophysical properties using range of techniques were carried out for harvested, untreated gum Arabic from Sudan-savannah ecological zone of Nigeria. The results revealed that it has some qualities which were in agreement with the international standard specified for gum Arabic. The soil type of the study area was found to be clayey-sandy in nature.
The root bark of Massularia acuminata has been used for medicinal purpose in Akwa Ibom State, Nig... more The root bark of Massularia acuminata has been used for medicinal purpose in Akwa Ibom State, Nigeria. The plant part has been established to be rich in some phytochemicals. This work evaluates the free radical scavenging ability and hence, antioxidant property of ethyl acetate, acetone, ethanol and methanol extracts of the plant part by the DPPH free radical scavenging method. Ethyl acetate extract exhibited the least free radical scavenging ability of 49.71±0.17 % at 100μg/ml concentration. But methanol, ethanol and acetone extracts showed appreciable scavenging ability of 67.47±0.30, 65.01±0.15 and 63.64±0.17 % respectively, values which are comparable with that of the common antioxidant standard, ascorbic acid. Therefore the three extracts can be said to possess reasonable antioxidant property. The variation in the free radical scavenging ability of the extracts shows that extracting solvents play a role in the types of phytochemicals extracted and hence antioxidant property. Based on the outcome of this work, the plant part can be said to possess antioxidant phytochemicals which can be used to manage oxidative stress.
Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used p... more Plant gum exudate harvested from Acacia senegal (Acacia gum/gum Arabic) is the most widely used plant gum especially in food and pharmaceutical industries due to its non toxicity and excellent properties as emulsifiers and stabilizers in emulsions. It consists of complex polysaccharides and glycoprotein, and its qualities are defined by the biophysical properties of the gum. Analyses of some biophysical properties using range of techniques were carried out for harvested, untreated gum Arabic from Sudan-savannah ecological zone of Nigeria. The results revealed that it has some qualities which were in agreement with the international standard specified for gum Arabic. The soil type of the study area was found to be clayey-sandy in nature.
ABSTRACT A triterpenoid saponin was isolated from the methanol extract of the root bark of Massul... more ABSTRACT
A triterpenoid saponin was isolated from the methanol extract of the root bark of Massularia acuminata spp. The
compound was identified as 3-O – β- D- glucopyranosyl – (1-4)- β- D- glucopyranosyl – (1-4)- β- Dglucopyranosyl-
olean -12-en-28-oic acid on the basis of chemical and spectral evidence of IR and NMR (1H, 13C,
DEPT, 1H-1H COSY, HSQC and HMBC) data. The compound showed good inhibitory activity against the
microorganisms, Salmonella spp, Staphylococcus aureus, Escherichia coli, Aspergillus niger and Candida albicans
indicating that it is a bioactive compound. It was also found to possess antioxidant property by virtue of its high
DPPH free radical scavenging ability of 73.09% at 100μg/ml, property that can make it relevant in stress
management and cosmetic industry.
KEYWORDS: Massularia acuminata, Triterpenoid saponins, Antimicrobial, Antioxidant, Isolation, Glycoside,
Spectroscopy.
Onion is one of the oldest cultivated bulb producing plant species and second most valuable veget... more Onion is one of the oldest cultivated bulb producing plant species and second most valuable vegetable after tomatoes in the world but is subject to great production loss due to its poor storability. Refractometric analysis was carried on some randomly selected production stock of onion bulbs in Bade district of Yobe state, Nigeria. This is because, soluble solid constitute most of the content of the onion bulb and dehydrator onion cultivars are developed from lines with high dry matter contents. Although dehydration is considered as one of the oldest and important method of onion processing for storage, it is yet to be utilised in this part of the world. The results of the study showed that different cultivars have different amount of soluble solid determined by their refractive index values which varies considerably and that smaller and medium size bulbs have more dry matter content than larger bulbs. The local white and red onion bulb cultivars have the potentials for dehydration compare to the other cultivars within the location of the study area.
The root bark extracts of Massularia acuminata from Akwa Ibom State, Nigeria, (locally known as "... more The root bark extracts of Massularia acuminata from Akwa Ibom State, Nigeria, (locally known as " okok edi ") have been investigated for its phytochemical constituents and antimicrobial activities. Air dried pulverized root bark of the plant part was sequentially solvent extracted with hexane, chloroform, ethyl acetate, acetone, ethanol and methanol. The phytochemical screening of the extracts show the presence of alkaloids, anthraquinones, saponins, phlobatannins, cardiac glycosides, tannins, terpenoids, steroids, flavonoids and resins, but not across all the extracts. Ethyl acetate, acetone, ethanol and methanol extracts were assayed for their antimicrobial activities and all were found to be active against the clinical isolates, Salmonella spp. Staphylococcus aureus, Eschurichia coli, Aspergillus nigger and Candida albicans but at varied level. The presence of some phytochemicals with known pharmacological effects in the plant part extracts and their antimicrobial activities justify the traditional use of the plant part for medicinal purpose.
ABSTRACT Fish contains fats/oils as part of their nutrients which undergo rancidity just as other... more ABSTRACT
Fish contains fats/oils as part of their nutrients which undergo rancidity just as
other oils. The rancidity of oils within fishes denotes fish spoilage. In this work,
time course monitoring of oil rancidity in five fish species; tilapia, mud, cat,
elephant snout and butter fishes harvested from Hadejia-Nguru wetland was carried
out by the thiobarbituric acid (TBA) value method to know their keeping quality. It
was found that, at six hour keeping, elephant snout, Mud and butter fishes were no
longer fresh based on their respective TBA values of 7.40, 7.10 and 6.50
mgMA/kg. The TBA values of 9.50, 11.0 and 8.50 mgMA/kg, respectively for
butter, elephant snout, and mud fishes at twelve hours of keeping indicated that
they have spoiled, while Tilapia and cat fishes with TBA values of 6.60 and
8,00mgMA/kg, respectively at the same twelve hours of keeping show the fishes to
have better keeping quality than the other three species. Generally, preservative
measures are required for cat, tilapia and mud fishes within nine hours of storage
and elephant snout and butter fishes need preservative measures immediately after
harvest for their long keeping.