Maithilee Kesralikar | Gulbarga University (original) (raw)

Papers by Maithilee Kesralikar

International Journal of Current Microbiology and Applied Sciences, Dec 10, 2021

Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are over 200 types ... more Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are over 200 types of cancers involves uncontrolled cell division and metastasis forming neoplasm and mass of undifferentiated cells that is tumor is a group of cells that have undergone unregulated growth which will often form a mass of tissue, but may be distributed slowly. All tumor cells show some of the common characteristics like continuous growth and division even given contrary signals, absence of programmed cell death, uncontrolled cell

International Journal of Current Microbiology and Applied Sciences, Jun 10, 2017

Onion (Allium cepa L.) (2n =16) is one of the important bulb producing vegetable crops. The knowl... more Onion (Allium cepa L.) (2n =16) is one of the important bulb producing vegetable crops. The knowledge of genetic diversity in a crop species is fundamental to its improvement. However, morphological traits have many limitations, including low polymorphism, low heritability, late expression, and may be controlled by epistatic and pleiotropic gene effects (Cramer and Havey, 1999). Now a day’s very powerful PCR-based techniques have also emerged which are very fast, reliable and require minimal amount of tissue for investigation. The use of molecular markers for the evaluation of genetic diversity is receiving much attention than morphological characterization. The various marker tools are Randomly Amplified Polymorphic DNA (RAPD), Simple Sequence Repeats (SSR), Inter Simple Sequence Repeats (ISSR) and Sequence Related Amplified Polymorphism (SRAP). They have large number of applications like characterization of gene pool, DNA fingerprinting, phylogenetic analysis, molecular dissection of complex traits, and characterization of genome organization. These DNA markers offer several advantages over traditional phenotypic markers, as they provide data that can be analysed objectively. The knowledge acquired through this investigation may play a pivotal role in the application of molecular markers in onion improvement programs.

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four-gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were disc diffusion tested for antibacterial activity. The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were used to evaluate antioxidant activity. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug in vitro. Tests for antioxidant activity were conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to include flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study as were thirty other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments performed in the lab revealed the compounds' potent inhibition activity. Analysis of the GC-MS data revealed the existence of thirty distinct components. MTT assay on the extract revealed anticancer activity. The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022), Dec 13, 2022

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four-gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were disc diffusion tested for antibacterial activity. The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were used to evaluate antioxidant activity. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug in vitro. Tests for antioxidant activity were conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to include flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study as were thirty other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments performed in the lab revealed the compounds' potent inhibition activity. Analysis of the GC-MS data revealed the existence of thirty distinct components. MTT assay on the extract revealed anticancer activity. The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

Preliminary phytochemical screening of Stachytarpheta mutabilis with methanol revealed the presen... more Preliminary phytochemical screening of Stachytarpheta mutabilis with methanol revealed the presence of various phytocomponents such as phenols, flavonoids, steroids, saponins, alkaloids, tannins, terpenoids, etc. In vitro anti-inflammatory activity was assessed using a nitric oxide scavenging test at various concentrations. Indomethacin was used as the standard drug. The results showed that Stachytarphea mutabilis Methanol Extract (SMME) exhibited significant antiinflammatory activity in vitro over a concentration range of 100-500 g/mL. The study aimed to evaluate anti-inflammatory activity in vivo in rats. The results obtained in the present study indicate that methanol extracts from Stachytarphea mutabilis can be a potential source of anti-inflammatory agent. In vitro anti-inflammatory study showed 44.19 % and in vivo studies showed that 42 % activity.

Acta Scientific Microbiology

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. This species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were performed by disc diffusion tested for antibacterial activity .The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were conducted. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug at in vitro. Tests for antioxidant activity was conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) , and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to consist flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study indicated other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments were performed in the lab revealed potent inhibition activity of the compound. Analysis of the GC-MS data has revealed the existence of thirty distinct components. MTT assay on the extract indicated anticancer activities of The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

The experimental material comprised of 16 Genotypes of onion. The 2X CTAB extraction buffer was p... more The experimental material comprised of 16 Genotypes of onion. The 2X CTAB extraction buffer was prepared by using components (2%CTAB, 5M NaCl, 1M Tris, 0.5 M EDTA)

The plants have tremendous medicinal properties. Some plants are being unnoticed though they have... more The plants have tremendous medicinal properties. Some plants are being unnoticed though they have some significant medicinal advantages. Stachytarpheta mutabilis belongs to verbenaceae family. It has different names changeable velvetberry, coral porterweed, pink snakeweed, red snakeweed. It is native to Mexico, the Caribbean, and South America and in Western parts of Maharashtra, South Karnataka and India.The other species of Stachytarpheta are showing other medicinal properties such as antimicrobial, anti-inflammatory and anticancer properties.(Ololade et al 2014) The phytochemical analysis from leaves of Stachytarpheta mutabilis study investigated presence of secondary metabolites such as Phenols, flavonoids, steroids, tannins, glycosides, terpenoids, saponins and alkaloids. (Irenus et al 2018). The proximate analysis parameters such as total ash (14.92%), acid insoluble ash (3%), water insoluble ash (3%), alcohol extractive index (1.4%), water extractive index (1.24%), moisture content (8%) were evaluated (Cyril ogbiko, Dickson musa et al 2019). Quantitative estimation of total phenols(300µg/mg), total tannins(100µg/mg), total flavonoids(25µg/mg), and total terpenoids(120µg/mg) comparing with gallic acid, tannic acid, quercetin, and linalool equivalent respectively were evaluated (Iwu Irenus Chinonye, Onu Uchenna Lynda 2018) The study recommends for further research analysis on active compounds and other additional medicinal properties

An experimental research was conducted among the 16 garlic genotypes for analysis of genetic dive... more An experimental research was conducted among the 16 garlic genotypes for analysis of genetic diversity. The present investigation on “Genetic diversity analysis among garlic (Allium sativum L.) genotypes through molecular markers” was planned to conduct with objective of DNA fingerprinting through RAPD, ISSR and SSR primers. RAPD ISSR and SSR markers are used for DNA fingerprinting. Among all markers ISSR and SSR markers gave diversified results than RAPD.

International Journal of Current Microbiology and Applied Sciences, 2021

Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are different types... more Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are different types of cancers involves uncontrolled cell division and metastasis forming neoplasm and mass of undifferentiated cells that is tumor is a group of cells that have undergone unregulated growth which will often form a mass of tissue, but may be distributed slowly. Recent studies indicate that, the gut micro biome has emerged as an important mediating factor of health and disease. Interactions between microbes and human cells play very important roles in human metabolism including digestion of biomolecules like carbohydrates, production of essential amino acids, beneficial fatty acid synthesis and vitamin compounds. During cancer progression, these metabolic interactions between microbes and human cells may shift from one that support health to another that threatens it, as microbes begin interacting with cancer cells rather than healthy human cells. Indeed, microbial dysbiosis (disruption to t...

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. This species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were performed by disc diffusion tested for antibacterial activity .The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were conducted. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug at in vitro. Tests for antioxidant activity was conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) , and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to consist flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study indicated other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments were performed in the lab revealed potent inhibition activity of the compound. Analysis of the GC-MS data has revealed the existence of thirty distinct components. MTT assay on the extract indicated anticancer activities of The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

International Journal of Current Microbiology and Applied Sciences, Dec 10, 2021

Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are over 200 types ... more Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are over 200 types of cancers involves uncontrolled cell division and metastasis forming neoplasm and mass of undifferentiated cells that is tumor is a group of cells that have undergone unregulated growth which will often form a mass of tissue, but may be distributed slowly. All tumor cells show some of the common characteristics like continuous growth and division even given contrary signals, absence of programmed cell death, uncontrolled cell

International Journal of Current Microbiology and Applied Sciences, Jun 10, 2017

Onion (Allium cepa L.) (2n =16) is one of the important bulb producing vegetable crops. The knowl... more Onion (Allium cepa L.) (2n =16) is one of the important bulb producing vegetable crops. The knowledge of genetic diversity in a crop species is fundamental to its improvement. However, morphological traits have many limitations, including low polymorphism, low heritability, late expression, and may be controlled by epistatic and pleiotropic gene effects (Cramer and Havey, 1999). Now a day’s very powerful PCR-based techniques have also emerged which are very fast, reliable and require minimal amount of tissue for investigation. The use of molecular markers for the evaluation of genetic diversity is receiving much attention than morphological characterization. The various marker tools are Randomly Amplified Polymorphic DNA (RAPD), Simple Sequence Repeats (SSR), Inter Simple Sequence Repeats (ISSR) and Sequence Related Amplified Polymorphism (SRAP). They have large number of applications like characterization of gene pool, DNA fingerprinting, phylogenetic analysis, molecular dissection of complex traits, and characterization of genome organization. These DNA markers offer several advantages over traditional phenotypic markers, as they provide data that can be analysed objectively. The knowledge acquired through this investigation may play a pivotal role in the application of molecular markers in onion improvement programs.

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four-gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were disc diffusion tested for antibacterial activity. The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were used to evaluate antioxidant activity. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug in vitro. Tests for antioxidant activity were conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to include flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study as were thirty other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments performed in the lab revealed the compounds' potent inhibition activity. Analysis of the GC-MS data revealed the existence of thirty distinct components. MTT assay on the extract revealed anticancer activity. The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022), Dec 13, 2022

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four-gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were disc diffusion tested for antibacterial activity. The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were used to evaluate antioxidant activity. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug in vitro. Tests for antioxidant activity were conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to include flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study as were thirty other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments performed in the lab revealed the compounds' potent inhibition activity. Analysis of the GC-MS data revealed the existence of thirty distinct components. MTT assay on the extract revealed anticancer activity. The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

Preliminary phytochemical screening of Stachytarpheta mutabilis with methanol revealed the presen... more Preliminary phytochemical screening of Stachytarpheta mutabilis with methanol revealed the presence of various phytocomponents such as phenols, flavonoids, steroids, saponins, alkaloids, tannins, terpenoids, etc. In vitro anti-inflammatory activity was assessed using a nitric oxide scavenging test at various concentrations. Indomethacin was used as the standard drug. The results showed that Stachytarphea mutabilis Methanol Extract (SMME) exhibited significant antiinflammatory activity in vitro over a concentration range of 100-500 g/mL. The study aimed to evaluate anti-inflammatory activity in vivo in rats. The results obtained in the present study indicate that methanol extracts from Stachytarphea mutabilis can be a potential source of anti-inflammatory agent. In vitro anti-inflammatory study showed 44.19 % and in vivo studies showed that 42 % activity.

Acta Scientific Microbiology

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. This species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were performed by disc diffusion tested for antibacterial activity .The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were conducted. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug at in vitro. Tests for antioxidant activity was conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) , and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to consist flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study indicated other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments were performed in the lab revealed potent inhibition activity of the compound. Analysis of the GC-MS data has revealed the existence of thirty distinct components. MTT assay on the extract indicated anticancer activities of The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.

The experimental material comprised of 16 Genotypes of onion. The 2X CTAB extraction buffer was p... more The experimental material comprised of 16 Genotypes of onion. The 2X CTAB extraction buffer was prepared by using components (2%CTAB, 5M NaCl, 1M Tris, 0.5 M EDTA)

The plants have tremendous medicinal properties. Some plants are being unnoticed though they have... more The plants have tremendous medicinal properties. Some plants are being unnoticed though they have some significant medicinal advantages. Stachytarpheta mutabilis belongs to verbenaceae family. It has different names changeable velvetberry, coral porterweed, pink snakeweed, red snakeweed. It is native to Mexico, the Caribbean, and South America and in Western parts of Maharashtra, South Karnataka and India.The other species of Stachytarpheta are showing other medicinal properties such as antimicrobial, anti-inflammatory and anticancer properties.(Ololade et al 2014) The phytochemical analysis from leaves of Stachytarpheta mutabilis study investigated presence of secondary metabolites such as Phenols, flavonoids, steroids, tannins, glycosides, terpenoids, saponins and alkaloids. (Irenus et al 2018). The proximate analysis parameters such as total ash (14.92%), acid insoluble ash (3%), water insoluble ash (3%), alcohol extractive index (1.4%), water extractive index (1.24%), moisture content (8%) were evaluated (Cyril ogbiko, Dickson musa et al 2019). Quantitative estimation of total phenols(300µg/mg), total tannins(100µg/mg), total flavonoids(25µg/mg), and total terpenoids(120µg/mg) comparing with gallic acid, tannic acid, quercetin, and linalool equivalent respectively were evaluated (Iwu Irenus Chinonye, Onu Uchenna Lynda 2018) The study recommends for further research analysis on active compounds and other additional medicinal properties

An experimental research was conducted among the 16 garlic genotypes for analysis of genetic dive... more An experimental research was conducted among the 16 garlic genotypes for analysis of genetic diversity. The present investigation on “Genetic diversity analysis among garlic (Allium sativum L.) genotypes through molecular markers” was planned to conduct with objective of DNA fingerprinting through RAPD, ISSR and SSR primers. RAPD ISSR and SSR markers are used for DNA fingerprinting. Among all markers ISSR and SSR markers gave diversified results than RAPD.

International Journal of Current Microbiology and Applied Sciences, 2021

Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are different types... more Cancer is the uncontrolled growth of abnormal cells anywhere in a body. There are different types of cancers involves uncontrolled cell division and metastasis forming neoplasm and mass of undifferentiated cells that is tumor is a group of cells that have undergone unregulated growth which will often form a mass of tissue, but may be distributed slowly. Recent studies indicate that, the gut micro biome has emerged as an important mediating factor of health and disease. Interactions between microbes and human cells play very important roles in human metabolism including digestion of biomolecules like carbohydrates, production of essential amino acids, beneficial fatty acid synthesis and vitamin compounds. During cancer progression, these metabolic interactions between microbes and human cells may shift from one that support health to another that threatens it, as microbes begin interacting with cancer cells rather than healthy human cells. Indeed, microbial dysbiosis (disruption to t...

In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervai... more In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. This species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were performed by disc diffusion tested for antibacterial activity .The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were conducted. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug at in vitro. Tests for antioxidant activity was conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) , and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to consist flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study indicated other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments were performed in the lab revealed potent inhibition activity of the compound. Analysis of the GC-MS data has revealed the existence of thirty distinct components. MTT assay on the extract indicated anticancer activities of The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.