Alona Biketova | University of Haifa (original) (raw)

Uploads

Conference Presentations by Alona Biketova

Papers by Alona Biketova

Cloning genes and operons encoding heterologous functions in bacterial hosts is almost exclusivel... more Cloning genes and operons encoding heterologous functions in bacterial hosts is almost exclusively carried out today using plasmid vectors. This has multiple drawbacks, including the need for constant selection and the variation in copy numbers. Chromosomal integration of transgenes has always offered a viable alternative, however, to date it has been of limited use due to its tedious nature and to being limited often to a single copy. We introduce here a strategy that uses bacterial insertion sequences, the simplest autonomous transposable elements to insert and amplify genetic cargo into a bacterial chromosome. Transgene insertion can take place either as transposition or homologous recombination, and copy-number amplification is achieved using controlled copy-paste transposition. We display successful use of IS1 and IS3 for this purpose in Escherichia coli cells, using various selection markers. We demonstrate the insertion of selectable genes, an unselectable gene, and a five-ge...

Journal of Fungi, 2022

The boletoid genera Butyriboletus and Exsudoporus have recently been suggested by some researcher... more The boletoid genera Butyriboletus and Exsudoporus have recently been suggested by some researchers to constitute a single genus, and Exsudoporus was merged into Butyriboletus as a later synonym. However, no convincing arguments have yet provided significant evidence for this congeneric placement. In this study, we analyze material from Exsudoporus species and closely related taxa to assess taxonomic and phylogenetic boundaries between these genera and to clarify species delimitation within Exsudoporus. Outcomes from a multilocus phylogenetic analysis (ITS, nrLSU, tef1-α and rpb2) clearly resolve Exsudoporus as a monophyletic, homogenous and independent genus that is sister to Butyriboletus. An accurate morphological description, comprehensive sampling, type studies, line drawings and a historical overview on the nomenclatural issues of the type species E. permagnificus are provided. Furthermore, this species is documented for the first time from Israel in association with Quercus ca...

Mikologiya I Fitopatologiya

The paper provides information on the first record of the rare and remarkable boletoid fungus Rub... more The paper provides information on the first record of the rare and remarkable boletoid fungus Rubinoboletus rubinus (W.G. Sm.) Pilát et Dermek in Ukraine (Vinnitsa). A short diagnosis, distribution, ecology and photographs of the spores and basidiomata, and also GeneBank accession number of ITS sequence of this sample are provided. The potential conservation status of this species is briefly discussed.

Persoonia : Molecular Phylogeny and Evolution of Fungi, 2019

Novel species of fungi described in this study include those from various countries as follows: A... more Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernam...

Some of the authors of this publication are also working on these related projects: Herbario TFC ... more Some of the authors of this publication are also working on these related projects: Herbario TFC View project DNA barcoding for plant-pathogens diagnostic and monitoring. Forest diseases and turbo-taxonomy in Tympanidaceae as a case of study View project

IMA fungus, 2018

Nomenclatural type definitions are one of the most important concepts in biological nomenclature.... more Nomenclatural type definitions are one of the most important concepts in biological nomenclature. Being physical objects that can be re-studied by other researchers, types permanently link taxonomy (an artificial agreement to classify biological diversity) with nomenclature (an artificial agreement to name biological diversity). Two proposals to amend the International Code of Nomenclature for algae, fungi, and plants (ICN), allowing DNA sequences alone (of any region and extent) to serve as types of taxon names for voucherless fungi (mainly putative taxa from environmental DNA sequences), have been submitted to be voted on at the 11 International Mycological Congress (Puerto Rico, July 2018). We consider various genetic processes affecting the distribution of alleles among taxa and find that alleles may not consistently and uniquely represent the species within which they are contained. Should the proposals be accepted, the meaning of nomenclatural types would change in a fundament...

The paper provides information on the first record of the rare and remarkable boletoid fungus Rub... more The paper provides information on the first record of the rare and remarkable boletoid fungus Rubinoboletus rubinus (W.G. Sm.) Pilát et Dermek in Ukraine (Vinnitsa). A short diagnosis, distribution, ecology and photographs of the spores and basidiomata, and also GeneBank accession number of ITS sequence of this sample are provided. The potential conservation status of this species is briefly discussed.

This paper presents information about Boletus poikilochromus, B. pulchrotinctus, and B. pulverule... more This paper presents information about Boletus poikilochromus, B. pulchrotinctus, and B. pulverulentus in Israel. These species are reported for the first time in Israel, and also B. pulchrotinctus and B. poikilochromus are reported for the first time in Asia. Furthermore, these three species are reported for the first time in association with Quercus calliprinos. Macro- and microscopical characteristics, distribution, ecology, and illustrations related to morphology and anatomy of the species are provided.

Cloning genes and operons encoding heterologous functions in bacterial hosts is almost exclusivel... more Cloning genes and operons encoding heterologous functions in bacterial hosts is almost exclusively carried out today using plasmid vectors. This has multiple drawbacks, including the need for constant selection and the variation in copy numbers. Chromosomal integration of transgenes has always offered a viable alternative, however, to date it has been of limited use due to its tedious nature and to being limited often to a single copy. We introduce here a strategy that uses bacterial insertion sequences, the simplest autonomous transposable elements to insert and amplify genetic cargo into a bacterial chromosome. Transgene insertion can take place either as transposition or homologous recombination, and copy-number amplification is achieved using controlled copy-paste transposition. We display successful use of IS1 and IS3 for this purpose in Escherichia coli cells, using various selection markers. We demonstrate the insertion of selectable genes, an unselectable gene, and a five-ge...

Journal of Fungi, 2022

The boletoid genera Butyriboletus and Exsudoporus have recently been suggested by some researcher... more The boletoid genera Butyriboletus and Exsudoporus have recently been suggested by some researchers to constitute a single genus, and Exsudoporus was merged into Butyriboletus as a later synonym. However, no convincing arguments have yet provided significant evidence for this congeneric placement. In this study, we analyze material from Exsudoporus species and closely related taxa to assess taxonomic and phylogenetic boundaries between these genera and to clarify species delimitation within Exsudoporus. Outcomes from a multilocus phylogenetic analysis (ITS, nrLSU, tef1-α and rpb2) clearly resolve Exsudoporus as a monophyletic, homogenous and independent genus that is sister to Butyriboletus. An accurate morphological description, comprehensive sampling, type studies, line drawings and a historical overview on the nomenclatural issues of the type species E. permagnificus are provided. Furthermore, this species is documented for the first time from Israel in association with Quercus ca...

Mikologiya I Fitopatologiya

The paper provides information on the first record of the rare and remarkable boletoid fungus Rub... more The paper provides information on the first record of the rare and remarkable boletoid fungus Rubinoboletus rubinus (W.G. Sm.) Pilát et Dermek in Ukraine (Vinnitsa). A short diagnosis, distribution, ecology and photographs of the spores and basidiomata, and also GeneBank accession number of ITS sequence of this sample are provided. The potential conservation status of this species is briefly discussed.

Persoonia : Molecular Phylogeny and Evolution of Fungi, 2019

Novel species of fungi described in this study include those from various countries as follows: A... more Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernam...

Some of the authors of this publication are also working on these related projects: Herbario TFC ... more Some of the authors of this publication are also working on these related projects: Herbario TFC View project DNA barcoding for plant-pathogens diagnostic and monitoring. Forest diseases and turbo-taxonomy in Tympanidaceae as a case of study View project

IMA fungus, 2018

Nomenclatural type definitions are one of the most important concepts in biological nomenclature.... more Nomenclatural type definitions are one of the most important concepts in biological nomenclature. Being physical objects that can be re-studied by other researchers, types permanently link taxonomy (an artificial agreement to classify biological diversity) with nomenclature (an artificial agreement to name biological diversity). Two proposals to amend the International Code of Nomenclature for algae, fungi, and plants (ICN), allowing DNA sequences alone (of any region and extent) to serve as types of taxon names for voucherless fungi (mainly putative taxa from environmental DNA sequences), have been submitted to be voted on at the 11 International Mycological Congress (Puerto Rico, July 2018). We consider various genetic processes affecting the distribution of alleles among taxa and find that alleles may not consistently and uniquely represent the species within which they are contained. Should the proposals be accepted, the meaning of nomenclatural types would change in a fundament...

The paper provides information on the first record of the rare and remarkable boletoid fungus Rub... more The paper provides information on the first record of the rare and remarkable boletoid fungus Rubinoboletus rubinus (W.G. Sm.) Pilát et Dermek in Ukraine (Vinnitsa). A short diagnosis, distribution, ecology and photographs of the spores and basidiomata, and also GeneBank accession number of ITS sequence of this sample are provided. The potential conservation status of this species is briefly discussed.

This paper presents information about Boletus poikilochromus, B. pulchrotinctus, and B. pulverule... more This paper presents information about Boletus poikilochromus, B. pulchrotinctus, and B. pulverulentus in Israel. These species are reported for the first time in Israel, and also B. pulchrotinctus and B. poikilochromus are reported for the first time in Asia. Furthermore, these three species are reported for the first time in association with Quercus calliprinos. Macro- and microscopical characteristics, distribution, ecology, and illustrations related to morphology and anatomy of the species are provided.