Manabu Tokeshi | Hokkaido University (original) (raw)
Papers by Manabu Tokeshi
ACS Applied Bio Materials, 2021
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RSC Advances, 2021
We developed a three-dimensional and symmetrically assembled microfluidic device named the 3D-iLi... more We developed a three-dimensional and symmetrically assembled microfluidic device named the 3D-iLiNP device. The 3D-iLiNP device allowed the precise size control of sub-100 nm sized lipid nanoparticles by the homogeneous and slow ethanol dilution.
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ACS Omega, 2021
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This paper described a simple preparation method for small-size and monodispersed lipid nanoparti... more This paper described a simple preparation method for small-size and monodispersed lipid nanoparticles (LNPs) by using microfluidic devices. The fundamental role and importance of chaotic micromixer in the microfluidic device was demonstrated. The suitable cycle number of chaotic micromixer was confirmed for precise controlling LNPs size with narrow distribution under the any flow rate conditions. In addition, LNPs containing siRNA was synthesized for evaluation of penetration efficiency via in vivo experiment. The PEGylated LNPs containing siRNA with a diameter of 30 nm could penetrate to the mouse parenchymal liver cells rather than the LNPs with a diameter of 50 nm.
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Analytical Sciences, 2018
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Analytical Sciences, 2017
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ACS Omega, 2019
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Analytical Sciences, 2019
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ACS Omega, 2018
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Review of Scientific Instruments, 2018
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ACS Omega, 2018
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Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed,... more Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques such as electrochemical and optical detection have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.
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PloS one, 2017
Lipid nanoparticles (LNPs) or liposomes are the most widely used drug carriers for nanomedicines.... more Lipid nanoparticles (LNPs) or liposomes are the most widely used drug carriers for nanomedicines. The size of LNPs is one of the essential factors affecting drug delivery efficiency and therapeutic efficiency. Here, we demonstrated the effect of lipid concentration and mixing performance on the LNP size using microfluidic devices with the aim of understanding the LNP formation mechanism and controlling the LNP size precisely. We fabricated microfluidic devices with different depths, 11 μm and 31 μm, of their chaotic micromixer structures. According to the LNP formation behavior results, by using a low concentration of the lipid solution and the microfluidic device equipped with the 31 μm chaotic mixer structures, we were able to produce the smallest-sized LNPs yet with a narrow particle size distribution. We also evaluated the mixing rate of the microfluidic devices using a laser scanning confocal microscopy and we estimated the critical ethanol concentration for controlling the LNP...
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Bunseki kagaku, 2015
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The Analyst, Jan 28, 2016
The development of a competitive immunoassay system for colorimetric detection on microfluidic pa... more The development of a competitive immunoassay system for colorimetric detection on microfluidic paper-based analytical devices (μPADs) is reported. The μPADs were fabricated via photolithography to define hydrophilic flow channels and consisted of three main elements: the control and test zones, where target detection was performed, the sample introduction zone, and the competitive capture zone located between the sample introduction zone and the test zone. The chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) was deposited at the control and test zones. μPAD surface modification was performed at the capture zone first via chitosan activation, then the BSA-conjugated target compound was immobilized. The sample solution consisting of the target compound, the peroxidase-conjugated antibody, and the hydrogen peroxide oxidizing agent was introduced into the device and competition occurred at the capture zone, allowing only the target-bound peroxidase-conjugated antibody ...
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Analytical Sciences, 2016
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Analytical and bioanalytical chemistry, 2016
A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based o... more A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based on the spontaneous capillary action of paper and eliminates unbound antigen and antibody in a sandwich immunoassay is reported. Liquids can flow through a porous medium (such as paper) in the absence of external pressure as a result of capillary action. Uniform results were achieved when washing a paper substrate in a PDMS holder which was integrated with a cartridge absorber acting as a porous medium. Our study demonstrated that applying this washing technique would allow μPADs to become the least expensive microfluidic device platform with high reproducibility and sensitivity. In a model μPAD assay that utilized this novel washing technique, C-reactive protein (CRP) was detected with a limit of detection (LOD) of 5 μg mL(-1). Graphical Abstract A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based on the spontaneous capillary action of paper and...
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Analytical Sciences, 2016
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ACS Applied Bio Materials, 2021
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RSC Advances, 2021
We developed a three-dimensional and symmetrically assembled microfluidic device named the 3D-iLi... more We developed a three-dimensional and symmetrically assembled microfluidic device named the 3D-iLiNP device. The 3D-iLiNP device allowed the precise size control of sub-100 nm sized lipid nanoparticles by the homogeneous and slow ethanol dilution.
Bookmarks Related papers MentionsView impact
ACS Omega, 2021
Bookmarks Related papers MentionsView impact
This paper described a simple preparation method for small-size and monodispersed lipid nanoparti... more This paper described a simple preparation method for small-size and monodispersed lipid nanoparticles (LNPs) by using microfluidic devices. The fundamental role and importance of chaotic micromixer in the microfluidic device was demonstrated. The suitable cycle number of chaotic micromixer was confirmed for precise controlling LNPs size with narrow distribution under the any flow rate conditions. In addition, LNPs containing siRNA was synthesized for evaluation of penetration efficiency via in vivo experiment. The PEGylated LNPs containing siRNA with a diameter of 30 nm could penetrate to the mouse parenchymal liver cells rather than the LNPs with a diameter of 50 nm.
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Analytical Sciences, 2018
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Analytical Sciences, 2017
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ACS Omega, 2019
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Analytical Sciences, 2019
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ACS Omega, 2018
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Review of Scientific Instruments, 2018
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ACS Omega, 2018
Bookmarks Related papers MentionsView impact
Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed,... more Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques such as electrochemical and optical detection have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.
Bookmarks Related papers MentionsView impact
PloS one, 2017
Lipid nanoparticles (LNPs) or liposomes are the most widely used drug carriers for nanomedicines.... more Lipid nanoparticles (LNPs) or liposomes are the most widely used drug carriers for nanomedicines. The size of LNPs is one of the essential factors affecting drug delivery efficiency and therapeutic efficiency. Here, we demonstrated the effect of lipid concentration and mixing performance on the LNP size using microfluidic devices with the aim of understanding the LNP formation mechanism and controlling the LNP size precisely. We fabricated microfluidic devices with different depths, 11 μm and 31 μm, of their chaotic micromixer structures. According to the LNP formation behavior results, by using a low concentration of the lipid solution and the microfluidic device equipped with the 31 μm chaotic mixer structures, we were able to produce the smallest-sized LNPs yet with a narrow particle size distribution. We also evaluated the mixing rate of the microfluidic devices using a laser scanning confocal microscopy and we estimated the critical ethanol concentration for controlling the LNP...
Bookmarks Related papers MentionsView impact
Bunseki kagaku, 2015
Bookmarks Related papers MentionsView impact
The Analyst, Jan 28, 2016
The development of a competitive immunoassay system for colorimetric detection on microfluidic pa... more The development of a competitive immunoassay system for colorimetric detection on microfluidic paper-based analytical devices (μPADs) is reported. The μPADs were fabricated via photolithography to define hydrophilic flow channels and consisted of three main elements: the control and test zones, where target detection was performed, the sample introduction zone, and the competitive capture zone located between the sample introduction zone and the test zone. The chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) was deposited at the control and test zones. μPAD surface modification was performed at the capture zone first via chitosan activation, then the BSA-conjugated target compound was immobilized. The sample solution consisting of the target compound, the peroxidase-conjugated antibody, and the hydrogen peroxide oxidizing agent was introduced into the device and competition occurred at the capture zone, allowing only the target-bound peroxidase-conjugated antibody ...
Bookmarks Related papers MentionsView impact
Analytical Sciences, 2016
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Analytical and bioanalytical chemistry, 2016
A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based o... more A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based on the spontaneous capillary action of paper and eliminates unbound antigen and antibody in a sandwich immunoassay is reported. Liquids can flow through a porous medium (such as paper) in the absence of external pressure as a result of capillary action. Uniform results were achieved when washing a paper substrate in a PDMS holder which was integrated with a cartridge absorber acting as a porous medium. Our study demonstrated that applying this washing technique would allow μPADs to become the least expensive microfluidic device platform with high reproducibility and sensitivity. In a model μPAD assay that utilized this novel washing technique, C-reactive protein (CRP) was detected with a limit of detection (LOD) of 5 μg mL(-1). Graphical Abstract A novel washing technique for microfluidic paper-based analytical devices (μPADs) that is based on the spontaneous capillary action of paper and...
Bookmarks Related papers MentionsView impact
Analytical Sciences, 2016
Bookmarks Related papers MentionsView impact