Tânia Tavares | Oporto - Academia.edu (original) (raw)
Papers by Tânia Tavares
Hydrobiology, Nov 1, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Life, Jan 9, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Dairy Journal, Dec 1, 2011
El artículo seleccionado no se encuentra disponible por ahora a texto completo por no haber sido ... more El artículo seleccionado no se encuentra disponible por ahora a texto completo por no haber sido facilitado todavía por el investigador a cargo del archivo del mismo.
Molecules
The probiotic features of Lactiplantibacillus (L.) pentosus and L. paraplantarum strains, endogen... more The probiotic features of Lactiplantibacillus (L.) pentosus and L. paraplantarum strains, endogenous in Cobrançosa table olives from northeast Portugal, were assessed in terms of functional properties and health benefits. Fourteen lactic acid bacteria strains were compared with Lacticaseibacillus casei from a commercial brand of probiotic yoghurt and L. pentosus B281 from Greek probiotic table olives, in attempts to select strains with higher probiotic performances than those references. For functional properties, the i53 and i106 strains, respectively, exhibited: 22.2 ± 2.2% and 23.0 ± 2.2% for Caco-2 cell adhesion capacity; 21.6 ± 7.8% and 21.5 ± 1.4% for hydrophobicity; 93.0 ± 3.0% and 88.5 ± 4.5% for autoaggregation ability by 24 h of incubation; and ability to co-aggregate with selected pathogens—from 29 to 40% to Gram+ (e.g., Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212); and from 16 to 44% for Gram− (e.g., Escherichia coli ATCC 25922 and Salmonella en...
Life
Synechocystis salina is a cyanobacterium that has biotechnological potential thanks to its abilit... more Synechocystis salina is a cyanobacterium that has biotechnological potential thanks to its ability to synthesize several bioactive compounds of interest. Therefore, this study aimed to find optimal conditions, in terms of temperature (15–25 °C), pH (6.5–9.5), and NaCl concentration (10–40 g·L−1), using as objective functions the productivities of biomass, total carotenoids, total PBPs, phycocyanin (PC), allophycocyanin (APC), phycoerythrin (PE), and antioxidants (AOXs) capacity of Synechocystis salina (S. salina) strain LEGE 06155, based in factorial design resorting to Box-Behnken. The model predicted higher biomass productivities under a temperature of 25 °C, a pH of 7.5, and low NaCl concentrations (10 g·L−1). Maximum productivities in terms of bioactive compounds were attained at lower NaCl concentrations (10 g·L−1) (except for PE), with the best temperature and pH in terms of carotenoids and total and individual PBPs ranging from 23–25 °C to 7.5–9.5, respectively. PE was the on...
Life
Microalgae are known producers of antioxidant and anti-inflammatory compounds, making them natura... more Microalgae are known producers of antioxidant and anti-inflammatory compounds, making them natural alternatives to be used as food and feed functional ingredients. This study aimed to valorise biomass and exploit new applications and commercial value for four commercially available microalgae: Isochrysis galbana, Nannochloropsis sp., Tetraselmis sp., and Phaeodactylum tricornutum. For that, five extracts were obtained: acetone (A), ethanol (E), water (W), ethanol:water (EW). The antioxidant capacity (ABTS•+/DPPH•/•NO/O2•−/ORAC-FL) and anti-inflammatory capacity (HBRC/COX-2) of the extracts were screened. The general biochemical composition (carbohydrates, soluble proteins, and lipids) and the main groups of bioactive compounds (carotenoids, phenolic compounds, and peptides) of extracts were quantified. The results of antioxidant assays revealed the potential of some microalgae extracts: in ABTS•+, Nannochloropsis sp. E and Tetraselmis sp. A, E, and P; in DPPH•, Tetraselmis sp. A and...
Foods
Current market trends point at increasing demand for functional foods, namely those carrying prob... more Current market trends point at increasing demand for functional foods, namely those carrying probiotics. In the case of table olives, presence of probiotics would convey a competitive advantage to Mediterranean-based diets, already established for their cultural heritage and gastronomic character. This work assessed the safety and resistance to gastrointestinal digestion of 19 native LAB strains from Cobrançosa table olives. Strains were identified via molecular sequencing (4 fingerprints/10 strains for Lactiplantibacillus pentosus, and 2 fingerprints/9 strains for L. paraplantarum), and exposed to simulated gastrointestinal fluids, as per the INFOGEST in vitro protocol with modifications. None of those strains proved dangerous for human consumption. Survivability to the gastrointestinal resistance test ranged from 29% to 70%, with strain-dependent variability. L. paraplantarum i18, i27, and i102, and L. pentosus i10 and i11 exhibited statistically lower survival rates (29–35%) than...
Applied Sciences
Our major goal was the physicochemical, biochemical and microbiological characterization of Cobra... more Our major goal was the physicochemical, biochemical and microbiological characterization of Cobrançosa table olives, as support for the eventual granting of a PDO status. Seven producers were accordingly sampled throughout eleven months. Brines were analyzed for pH, salinity, acidity, and organic and phenolic compounds. Yeasts and Latic Acid Bacteria (LAB) were enumerated, and the dominant strains duly identified. Despite process variabilities, two stages appear to be shared by all manufacturers: sweetening—the renewal of water to remove bitter compounds; and salting—gradual addition of salt to brine for preservation. Yeasts dominated during sweetening, but LAB tended to be similar in viable counts (7 log CFU/mL) by the end of salting. Lactiplantibacillus (Lpb.) pentosus, Lpb. paraplantarum, Pediococcus parvulus, and Oenococcus kitaharae were the most abundant LAB found, together with an average pH of 4.1 and 6–9% for salt content. All organic acids exhibited an inverted parabolic e...
Bioengineering
Cyanobacteria are microorganisms that are well-adapted to sudden changes in their environment, na... more Cyanobacteria are microorganisms that are well-adapted to sudden changes in their environment, namely to light conditions. This has allowed them to develop mechanisms for photoprotection, which encompass alteration in pigment composition. Therefore, light modulation appears to be a suitable strategy to enhance the synthesis of specific pigments (e.g., phycocyanin) with commercial interest, in addition to conveying a more fundamental perspective on the mechanisms of acclimatization of cyanobacterium species. In this study, Synechocystis salina was accordingly cultivated in two light phase stages: (i) white LED, and (ii) shift to distinct light treatments, including white, green, and red LEDs. The type of LED lighting was combined with two intensities (50 and 150 µmolphotons·m−2·s−1). The effects on biomass production, photosynthetic efficiency, chlorophyll a (chl a) content, total carotenoids (and profile thereof), and phycobiliproteins (including phycocyanin, allophycocyanin, and ph...
![Research paper thumbnail of {"__content__"=>"Viability of dietary substitution of live microalgae with dry in broodstock conditioning of the Pacific oyster ().", "i"=>[{"__content__"=>"Ulva rigida"}, {"__content__"=>"Crassostrea gigas"}]}](https://attachments.academia-assets.com/113005701/thumbnails/1.jpg)
](Biology open, Jan 2, 2018
The current study evaluated the microalgae replacement by dry macroalgae () in the reproductive s... more The current study evaluated the microalgae replacement by dry macroalgae () in the reproductive success and biochemical composition of the Pacific oyster () during broodstock conditioning. Five nutritional regimes were tested: 100% macroalgae (diet 1), 50% macroalgae+50% microalgae (diet 2), 25% macroalgae+75% microalgae (diet 3) and 100% microalgae (diet 4). An unfed group was used as a negative control. The microalgae blend was composed of 33% and 67% diatoms (75% +25% ). Gonadal maturation was reflected in the physiological condition of the individuals. All treatments, except diet 1, showed an increase in condition index and were fully matured at the end of the trial, with the best physiological condition observed in oysters fed diet 3 and diet 4. Protein and total lipid content increased during the conditioning period, whereas glycogen content decreased. Oysters conditioned with diet 3 had higher protein and total lipid content and lower glycogen content than the other treatment...
International Dairy Journal, 2011
The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropep... more The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and a-La, but not from CMP. Maximum DH was 18% and 9%, for WPC and a-La, respectively. 50% ACE-inhibition was produced by 105.4 (total fraction) and 25.6 mg mL À1 (<3 kDa fraction) for WPC, and 47.6 (total fraction) and 22.5 mg mL À1 (<3 kDa fraction) for a-La. Major peptides of fractions exhibiting ACEinhibition were sequenced. The antioxidant activities of WPC and a-La were 0.96 AE 0.08 and 1.12 AE 0.13 mmol trolox equivalent per mg hydrolysed protein, respectively.
Journal of Applied Phycology
Microalgae have shown a great potential as a source of bioactive compounds, such lipidic compound... more Microalgae have shown a great potential as a source of bioactive compounds, such lipidic compounds as fatty acids and carotenoids, with recognized benefit for human health. Their synthesis is directly affected by culturing conditions, but, as photosynthetic organisms, microalgae are greatly influenced by light, particularly light quality and irradiance, responsible for growth regulation and activation of biochemical pathways. Therefore, the aim of this work was to maximize biomass and high added-value compound productivity by Scenedesmus obliquus M2-1, using different light irradiances of two previously selected light sources for the production of antioxidant compounds: monochromatic red light (R) and a mixture of 40% of blue and 60% of red light (BR). Four irradiances (50, 100, 150, and 200 μmolphotons m−2 s−1) were accordingly evaluated for both light sources, upon productivity of biomass, fatty acids, carotenoids, and total antioxidant compounds. Changes in light quality and irradiance influenced these aforementioned productivities. Optimal condition for biomass productivity was 150 μmolphotons m−2 s−1, regardless light quality, for both sources. On the other hand, when the production targets carotenoids, polyunsaturated fatty acids, and/or total antioxidant capacity, the optimal condition was found to be at 200 μmolphotons m−2 s−1 under BR light.
Raw Milk, 2019
Microbiological safety of raw milk is a highly controversial topic; however, consumer interest in... more Microbiological safety of raw milk is a highly controversial topic; however, consumer interest in raw milk products has been on the rise. Although thermal pasteurization affects bioavailability of raw milk nutritional components (and milk structure itself), nutrient deficiencies are hardly incurred in just from using raw instead of pasteurized milk. Some consumers prefer to drink raw milk for perceiving it as more “natural,” and thus possessing superior flavor and texture. In the latest decade, the growing interest in consumption of raw milk and products derived therefrom has motivated studies on alternative, minimal processing technologies that ensure quality and safety of raw milk and associated dairy products. This includes many products, ranging from traditional cheeses with more intense and diverse flavors, to different types of dairy products; note that pre- and probiotic fermented drinks, yoghurt, and ice cream may exhibit new flavors (e.g., fruity or caramel-like), besides i...
International Dairy Journal, 2013
Antinociceptive and anti-inflammatory effects of a peptide concentrate mix (PepC) obtained from w... more Antinociceptive and anti-inflammatory effects of a peptide concentrate mix (PepC) obtained from whey protein, via hydrolysis with cardosins from Cynara cardunculus, was tested in vivo. The antinociceptive effect was assessed using writhing, hot-plate and formalin tests in mice, and the anti-inflammatory effect using the paw oedema test. PepC at 300 mg kg−1 bw conveyed a significant result in the writhing test when co-administered with 1 and 3 mg kg−1 bw indomethacin, similar to administration of higher doses of indomethacin alone. Conversely, no statistically significant differences were observed in the paw oedema test when the same PepC concentration was co-administered with dexamethasone at 3, 10 and 30 mg kg−1 bw. PepC at 1000 mg kg−1 bw did not cause any remarkable outcome in the hot-plate test. PepC appears to possess anti-inflammatory and peripheral antinociceptive activities, so it may be a candidate for nutraceutical ingredient.
Journal of Applied Microbiology
Journal of Applied Phycology, 2021
A cyanobacterium of the genus Chroococcidiopsis has been recently unveiled to go to Mars, owing t... more A cyanobacterium of the genus Chroococcidiopsis has been recently unveiled to go to Mars, owing to its unique ability to stand harsh environmental conditions. Nevertheless, it has been little characterized in terms of other biotechnological applications. The aim of this study was thus to explore the biotechnological potential of Chroococcidiopsis sp. LEGE 06174, using GRAS (generally recognized as safe) solvents for the combined extraction of bioactive compounds. Due to its mucilaginous sheath, sulfuric acid, NaOH, PBS, DMSO 20%, DMSO 100%, and acetone were tested as preliminary treatment—in attempts to enhance pigment extraction. Extracts were then obtained by several series of successive extractions: phosphate-buffered saline (PBS) (serie A), ethanol-PBS (serie B), acetone-PBS (serie C), methanol-PBS (serie D), and acetone-methanol-PBS (serie E). Such extracts were screened for antioxidant capacity, cytotoxicity, and physicochemical characterization in terms of polysaccharides, pi...
Journal of Applied Phycology
Few studies have focused on the biotechnological potential of the marine cyanobacterium Synechocy... more Few studies have focused on the biotechnological potential of the marine cyanobacterium Synechocystis salina, in particular its bioactive metabolites. Hence, an opportunity exists to explore various solvent combinations in attempts to improve extraction of cellular contents of metabolites with antioxidant features as a part of a structured attempt to determine whether S. salina LEGE 06,155 holds a promise for industrial exploitation. Extracts were obtained by single extractions with four GRAS solvents: hexane (H), acetone (A), ethanol (E), and buffer phosphate (PBS) (series A) and successive extractions: H-E-PBS (series B), H-A-PBS (series C), and H-A-E-PBS (series D). The yields, antioxidant capacity, and cytotoxicity of the extracts, as well their content in pigments, lipids, and phenolic compounds were assessed. The combination of solvents of series B produced the highest yield of extraction, of 33.56%. Aqueous extracts for single (series A) and successive extractions (series B and C) presented the highest antioxidant capacity, for ABTS•+ and NO•−, respectively, and had a high content of phycobiliproteins (PBPs). The acetonic (series A) and ethanolic (series B) extracts also showed high antioxidant capacity, in particular for DPPH• and O2•−, respectively. Overall, organic extracts did not show signs of cytotoxicity. Series B extraction enhanced general pigment content, yet single extraction with ethanol (series A) produced the highest content in carotenoids, in particular echinenone and β-carotene, and PBS (series A) improved PBPs content. The lipid content was enhanced in series A (ethanol), while the total phenolic content was improved in series B. Synechocystis has accordingly demonstrated potential for eventual exploitation as biotechnological resource, in terms value-added compounds with antioxidant features.
Marine Drugs
Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and caroten... more Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and carotenoids, can avoid or treat oxidation-associated conditions and diseases like inflammation or cancer. This study aimed to assess the bioactive potential of lipidic extracts obtained from Gloeothece sp.–using Generally Recognized as Safe (GRAS) solvents like ethanol, acetone, hexane:isopropanol (3:2) (HI) and ethyl lactate. The bioactive potential of extracts was assessed in terms of antioxidant (ABTS•+, DPPH•, •NO and O2•assays), anti-inflammatory (HRBC membrane stabilization and Cox-2 screening assay), and antitumor capacity (death by TUNEL, and anti-proliferative by BrdU incorporation assay in AGS cancer cells); while its composition was characterized in terms of carotenoids and fatty acids, by HPLC-DAD and GC-FID methods, respectively. Results revealed a chemopreventive potential of the HI extract owing to its ability to: (I) scavenge -NO• radical (IC50, 1258 ± 0.353 µg·mL−1); (II) inhib...
Global Cheesemaking Technology
Molecules
One of the main goals of Mankind is to ensure food system sustainability—including management of ... more One of the main goals of Mankind is to ensure food system sustainability—including management of land, soil, water, and biodiversity. Microalgae accordingly appear as an innovative and scalable alternative source in view of the richness of their chemical profiles. In what concerns lipids in particular, microalgae can synthesize and accumulate significant amounts of fatty acids, a great fraction of which are polyunsaturated; this makes them excellent candidates within the framework of production and exploitation of lipids by various industrial and health sectors, either as bulk products or fine chemicals. Conventional lipid extraction methodologies require previous dehydration of microalgal biomass, which hampers economic feasibility due to the high energy demands thereof. Therefore, extraction of lipids directly from wet biomass would be a plus in this endeavor. Supporting processes and methodologies are still limited, and most approaches are empirical in nature—so a deeper mechanis...
Hydrobiology, Nov 1, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Life, Jan 9, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
International Dairy Journal, Dec 1, 2011
El artículo seleccionado no se encuentra disponible por ahora a texto completo por no haber sido ... more El artículo seleccionado no se encuentra disponible por ahora a texto completo por no haber sido facilitado todavía por el investigador a cargo del archivo del mismo.
Molecules
The probiotic features of Lactiplantibacillus (L.) pentosus and L. paraplantarum strains, endogen... more The probiotic features of Lactiplantibacillus (L.) pentosus and L. paraplantarum strains, endogenous in Cobrançosa table olives from northeast Portugal, were assessed in terms of functional properties and health benefits. Fourteen lactic acid bacteria strains were compared with Lacticaseibacillus casei from a commercial brand of probiotic yoghurt and L. pentosus B281 from Greek probiotic table olives, in attempts to select strains with higher probiotic performances than those references. For functional properties, the i53 and i106 strains, respectively, exhibited: 22.2 ± 2.2% and 23.0 ± 2.2% for Caco-2 cell adhesion capacity; 21.6 ± 7.8% and 21.5 ± 1.4% for hydrophobicity; 93.0 ± 3.0% and 88.5 ± 4.5% for autoaggregation ability by 24 h of incubation; and ability to co-aggregate with selected pathogens—from 29 to 40% to Gram+ (e.g., Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212); and from 16 to 44% for Gram− (e.g., Escherichia coli ATCC 25922 and Salmonella en...
Life
Synechocystis salina is a cyanobacterium that has biotechnological potential thanks to its abilit... more Synechocystis salina is a cyanobacterium that has biotechnological potential thanks to its ability to synthesize several bioactive compounds of interest. Therefore, this study aimed to find optimal conditions, in terms of temperature (15–25 °C), pH (6.5–9.5), and NaCl concentration (10–40 g·L−1), using as objective functions the productivities of biomass, total carotenoids, total PBPs, phycocyanin (PC), allophycocyanin (APC), phycoerythrin (PE), and antioxidants (AOXs) capacity of Synechocystis salina (S. salina) strain LEGE 06155, based in factorial design resorting to Box-Behnken. The model predicted higher biomass productivities under a temperature of 25 °C, a pH of 7.5, and low NaCl concentrations (10 g·L−1). Maximum productivities in terms of bioactive compounds were attained at lower NaCl concentrations (10 g·L−1) (except for PE), with the best temperature and pH in terms of carotenoids and total and individual PBPs ranging from 23–25 °C to 7.5–9.5, respectively. PE was the on...
Life
Microalgae are known producers of antioxidant and anti-inflammatory compounds, making them natura... more Microalgae are known producers of antioxidant and anti-inflammatory compounds, making them natural alternatives to be used as food and feed functional ingredients. This study aimed to valorise biomass and exploit new applications and commercial value for four commercially available microalgae: Isochrysis galbana, Nannochloropsis sp., Tetraselmis sp., and Phaeodactylum tricornutum. For that, five extracts were obtained: acetone (A), ethanol (E), water (W), ethanol:water (EW). The antioxidant capacity (ABTS•+/DPPH•/•NO/O2•−/ORAC-FL) and anti-inflammatory capacity (HBRC/COX-2) of the extracts were screened. The general biochemical composition (carbohydrates, soluble proteins, and lipids) and the main groups of bioactive compounds (carotenoids, phenolic compounds, and peptides) of extracts were quantified. The results of antioxidant assays revealed the potential of some microalgae extracts: in ABTS•+, Nannochloropsis sp. E and Tetraselmis sp. A, E, and P; in DPPH•, Tetraselmis sp. A and...
Foods
Current market trends point at increasing demand for functional foods, namely those carrying prob... more Current market trends point at increasing demand for functional foods, namely those carrying probiotics. In the case of table olives, presence of probiotics would convey a competitive advantage to Mediterranean-based diets, already established for their cultural heritage and gastronomic character. This work assessed the safety and resistance to gastrointestinal digestion of 19 native LAB strains from Cobrançosa table olives. Strains were identified via molecular sequencing (4 fingerprints/10 strains for Lactiplantibacillus pentosus, and 2 fingerprints/9 strains for L. paraplantarum), and exposed to simulated gastrointestinal fluids, as per the INFOGEST in vitro protocol with modifications. None of those strains proved dangerous for human consumption. Survivability to the gastrointestinal resistance test ranged from 29% to 70%, with strain-dependent variability. L. paraplantarum i18, i27, and i102, and L. pentosus i10 and i11 exhibited statistically lower survival rates (29–35%) than...
Applied Sciences
Our major goal was the physicochemical, biochemical and microbiological characterization of Cobra... more Our major goal was the physicochemical, biochemical and microbiological characterization of Cobrançosa table olives, as support for the eventual granting of a PDO status. Seven producers were accordingly sampled throughout eleven months. Brines were analyzed for pH, salinity, acidity, and organic and phenolic compounds. Yeasts and Latic Acid Bacteria (LAB) were enumerated, and the dominant strains duly identified. Despite process variabilities, two stages appear to be shared by all manufacturers: sweetening—the renewal of water to remove bitter compounds; and salting—gradual addition of salt to brine for preservation. Yeasts dominated during sweetening, but LAB tended to be similar in viable counts (7 log CFU/mL) by the end of salting. Lactiplantibacillus (Lpb.) pentosus, Lpb. paraplantarum, Pediococcus parvulus, and Oenococcus kitaharae were the most abundant LAB found, together with an average pH of 4.1 and 6–9% for salt content. All organic acids exhibited an inverted parabolic e...
Bioengineering
Cyanobacteria are microorganisms that are well-adapted to sudden changes in their environment, na... more Cyanobacteria are microorganisms that are well-adapted to sudden changes in their environment, namely to light conditions. This has allowed them to develop mechanisms for photoprotection, which encompass alteration in pigment composition. Therefore, light modulation appears to be a suitable strategy to enhance the synthesis of specific pigments (e.g., phycocyanin) with commercial interest, in addition to conveying a more fundamental perspective on the mechanisms of acclimatization of cyanobacterium species. In this study, Synechocystis salina was accordingly cultivated in two light phase stages: (i) white LED, and (ii) shift to distinct light treatments, including white, green, and red LEDs. The type of LED lighting was combined with two intensities (50 and 150 µmolphotons·m−2·s−1). The effects on biomass production, photosynthetic efficiency, chlorophyll a (chl a) content, total carotenoids (and profile thereof), and phycobiliproteins (including phycocyanin, allophycocyanin, and ph...
Biology open, Jan 2, 2018
The current study evaluated the microalgae replacement by dry macroalgae () in the reproductive s... more The current study evaluated the microalgae replacement by dry macroalgae () in the reproductive success and biochemical composition of the Pacific oyster () during broodstock conditioning. Five nutritional regimes were tested: 100% macroalgae (diet 1), 50% macroalgae+50% microalgae (diet 2), 25% macroalgae+75% microalgae (diet 3) and 100% microalgae (diet 4). An unfed group was used as a negative control. The microalgae blend was composed of 33% and 67% diatoms (75% +25% ). Gonadal maturation was reflected in the physiological condition of the individuals. All treatments, except diet 1, showed an increase in condition index and were fully matured at the end of the trial, with the best physiological condition observed in oysters fed diet 3 and diet 4. Protein and total lipid content increased during the conditioning period, whereas glycogen content decreased. Oysters conditioned with diet 3 had higher protein and total lipid content and lower glycogen content than the other treatment...
International Dairy Journal, 2011
The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropep... more The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and a-La, but not from CMP. Maximum DH was 18% and 9%, for WPC and a-La, respectively. 50% ACE-inhibition was produced by 105.4 (total fraction) and 25.6 mg mL À1 (<3 kDa fraction) for WPC, and 47.6 (total fraction) and 22.5 mg mL À1 (<3 kDa fraction) for a-La. Major peptides of fractions exhibiting ACEinhibition were sequenced. The antioxidant activities of WPC and a-La were 0.96 AE 0.08 and 1.12 AE 0.13 mmol trolox equivalent per mg hydrolysed protein, respectively.
Journal of Applied Phycology
Microalgae have shown a great potential as a source of bioactive compounds, such lipidic compound... more Microalgae have shown a great potential as a source of bioactive compounds, such lipidic compounds as fatty acids and carotenoids, with recognized benefit for human health. Their synthesis is directly affected by culturing conditions, but, as photosynthetic organisms, microalgae are greatly influenced by light, particularly light quality and irradiance, responsible for growth regulation and activation of biochemical pathways. Therefore, the aim of this work was to maximize biomass and high added-value compound productivity by Scenedesmus obliquus M2-1, using different light irradiances of two previously selected light sources for the production of antioxidant compounds: monochromatic red light (R) and a mixture of 40% of blue and 60% of red light (BR). Four irradiances (50, 100, 150, and 200 μmolphotons m−2 s−1) were accordingly evaluated for both light sources, upon productivity of biomass, fatty acids, carotenoids, and total antioxidant compounds. Changes in light quality and irradiance influenced these aforementioned productivities. Optimal condition for biomass productivity was 150 μmolphotons m−2 s−1, regardless light quality, for both sources. On the other hand, when the production targets carotenoids, polyunsaturated fatty acids, and/or total antioxidant capacity, the optimal condition was found to be at 200 μmolphotons m−2 s−1 under BR light.
Raw Milk, 2019
Microbiological safety of raw milk is a highly controversial topic; however, consumer interest in... more Microbiological safety of raw milk is a highly controversial topic; however, consumer interest in raw milk products has been on the rise. Although thermal pasteurization affects bioavailability of raw milk nutritional components (and milk structure itself), nutrient deficiencies are hardly incurred in just from using raw instead of pasteurized milk. Some consumers prefer to drink raw milk for perceiving it as more “natural,” and thus possessing superior flavor and texture. In the latest decade, the growing interest in consumption of raw milk and products derived therefrom has motivated studies on alternative, minimal processing technologies that ensure quality and safety of raw milk and associated dairy products. This includes many products, ranging from traditional cheeses with more intense and diverse flavors, to different types of dairy products; note that pre- and probiotic fermented drinks, yoghurt, and ice cream may exhibit new flavors (e.g., fruity or caramel-like), besides i...
International Dairy Journal, 2013
Antinociceptive and anti-inflammatory effects of a peptide concentrate mix (PepC) obtained from w... more Antinociceptive and anti-inflammatory effects of a peptide concentrate mix (PepC) obtained from whey protein, via hydrolysis with cardosins from Cynara cardunculus, was tested in vivo. The antinociceptive effect was assessed using writhing, hot-plate and formalin tests in mice, and the anti-inflammatory effect using the paw oedema test. PepC at 300 mg kg−1 bw conveyed a significant result in the writhing test when co-administered with 1 and 3 mg kg−1 bw indomethacin, similar to administration of higher doses of indomethacin alone. Conversely, no statistically significant differences were observed in the paw oedema test when the same PepC concentration was co-administered with dexamethasone at 3, 10 and 30 mg kg−1 bw. PepC at 1000 mg kg−1 bw did not cause any remarkable outcome in the hot-plate test. PepC appears to possess anti-inflammatory and peripheral antinociceptive activities, so it may be a candidate for nutraceutical ingredient.
Journal of Applied Microbiology
Journal of Applied Phycology, 2021
A cyanobacterium of the genus Chroococcidiopsis has been recently unveiled to go to Mars, owing t... more A cyanobacterium of the genus Chroococcidiopsis has been recently unveiled to go to Mars, owing to its unique ability to stand harsh environmental conditions. Nevertheless, it has been little characterized in terms of other biotechnological applications. The aim of this study was thus to explore the biotechnological potential of Chroococcidiopsis sp. LEGE 06174, using GRAS (generally recognized as safe) solvents for the combined extraction of bioactive compounds. Due to its mucilaginous sheath, sulfuric acid, NaOH, PBS, DMSO 20%, DMSO 100%, and acetone were tested as preliminary treatment—in attempts to enhance pigment extraction. Extracts were then obtained by several series of successive extractions: phosphate-buffered saline (PBS) (serie A), ethanol-PBS (serie B), acetone-PBS (serie C), methanol-PBS (serie D), and acetone-methanol-PBS (serie E). Such extracts were screened for antioxidant capacity, cytotoxicity, and physicochemical characterization in terms of polysaccharides, pi...
Journal of Applied Phycology
Few studies have focused on the biotechnological potential of the marine cyanobacterium Synechocy... more Few studies have focused on the biotechnological potential of the marine cyanobacterium Synechocystis salina, in particular its bioactive metabolites. Hence, an opportunity exists to explore various solvent combinations in attempts to improve extraction of cellular contents of metabolites with antioxidant features as a part of a structured attempt to determine whether S. salina LEGE 06,155 holds a promise for industrial exploitation. Extracts were obtained by single extractions with four GRAS solvents: hexane (H), acetone (A), ethanol (E), and buffer phosphate (PBS) (series A) and successive extractions: H-E-PBS (series B), H-A-PBS (series C), and H-A-E-PBS (series D). The yields, antioxidant capacity, and cytotoxicity of the extracts, as well their content in pigments, lipids, and phenolic compounds were assessed. The combination of solvents of series B produced the highest yield of extraction, of 33.56%. Aqueous extracts for single (series A) and successive extractions (series B and C) presented the highest antioxidant capacity, for ABTS•+ and NO•−, respectively, and had a high content of phycobiliproteins (PBPs). The acetonic (series A) and ethanolic (series B) extracts also showed high antioxidant capacity, in particular for DPPH• and O2•−, respectively. Overall, organic extracts did not show signs of cytotoxicity. Series B extraction enhanced general pigment content, yet single extraction with ethanol (series A) produced the highest content in carotenoids, in particular echinenone and β-carotene, and PBS (series A) improved PBPs content. The lipid content was enhanced in series A (ethanol), while the total phenolic content was improved in series B. Synechocystis has accordingly demonstrated potential for eventual exploitation as biotechnological resource, in terms value-added compounds with antioxidant features.
Marine Drugs
Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and caroten... more Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and carotenoids, can avoid or treat oxidation-associated conditions and diseases like inflammation or cancer. This study aimed to assess the bioactive potential of lipidic extracts obtained from Gloeothece sp.–using Generally Recognized as Safe (GRAS) solvents like ethanol, acetone, hexane:isopropanol (3:2) (HI) and ethyl lactate. The bioactive potential of extracts was assessed in terms of antioxidant (ABTS•+, DPPH•, •NO and O2•assays), anti-inflammatory (HRBC membrane stabilization and Cox-2 screening assay), and antitumor capacity (death by TUNEL, and anti-proliferative by BrdU incorporation assay in AGS cancer cells); while its composition was characterized in terms of carotenoids and fatty acids, by HPLC-DAD and GC-FID methods, respectively. Results revealed a chemopreventive potential of the HI extract owing to its ability to: (I) scavenge -NO• radical (IC50, 1258 ± 0.353 µg·mL−1); (II) inhib...
Global Cheesemaking Technology
Molecules
One of the main goals of Mankind is to ensure food system sustainability—including management of ... more One of the main goals of Mankind is to ensure food system sustainability—including management of land, soil, water, and biodiversity. Microalgae accordingly appear as an innovative and scalable alternative source in view of the richness of their chemical profiles. In what concerns lipids in particular, microalgae can synthesize and accumulate significant amounts of fatty acids, a great fraction of which are polyunsaturated; this makes them excellent candidates within the framework of production and exploitation of lipids by various industrial and health sectors, either as bulk products or fine chemicals. Conventional lipid extraction methodologies require previous dehydration of microalgal biomass, which hampers economic feasibility due to the high energy demands thereof. Therefore, extraction of lipids directly from wet biomass would be a plus in this endeavor. Supporting processes and methodologies are still limited, and most approaches are empirical in nature—so a deeper mechanis...