Željko Mihaljev - Academia.edu (original) (raw)
Papers by Željko Mihaljev
Sustainability
In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater ... more In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater from the slaughterhouse, and in this way integrates a new value chain in the form of purification and use of slaughterhouse wastewater in an aquaculture production system. The negative aspect of such integrated production systems is the concern related to the safety of fish meat produced in these systems. The aim of this research was to determine the activity level of Cs-137, K-40, Ra-226, Th-232, and Na-22 in water, sediment, and carp harvested from a pond that received purified water from a slaughterhouse. All samples were collected in spring and in autumn. The activity concentrations of selected radionuclides were determined by gamma spectrometry (HPG-e detector). The activity of K-40 in water samples ranged from 8.4 to 15.6 Bq L−1. The specific activity concentrations of the Ra-226 in water samples ranged between 1.02 ± 0.11 and 2.76 ± 0.49 Bq kg−1. The results of the activity of nat...
Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg ... more Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples w...
Sustainability
In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater ... more In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater from the slaughterhouse, and in this way integrates a new value chain in the form of purification and use of slaughterhouse wastewater in an aquaculture production system. The negative aspect of such integrated production systems is the concern related to the safety of fish meat produced in these systems. The aim of this research was to determine the activity level of Cs-137, K-40, Ra-226, Th-232, and Na-22 in water, sediment, and carp harvested from a pond that received purified water from a slaughterhouse. All samples were collected in spring and in autumn. The activity concentrations of selected radionuclides were determined by gamma spectrometry (HPG-e detector). The activity of K-40 in water samples ranged from 8.4 to 15.6 Bq L−1. The specific activity concentrations of the Ra-226 in water samples ranged between 1.02 ± 0.11 and 2.76 ± 0.49 Bq kg−1. The results of the activity of nat...
Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg ... more Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples w...
Archives of Veterinary Medicine
Providing healthy and safe food in terms of mycotoxicological safety is an imperative for not onl... more Providing healthy and safe food in terms of mycotoxicological safety is an imperative for not only good and sustainable livestock production, but also for the population that consumes food of both plant and animal origin. Climate change in the temperate regions of southern Europe has led to frequent occurrence of aflatoxins, deoxynivalenol and zearalenone in cereals. In order to reduce harmful effects of these toxins on animal health as well as to avoid large economic losses, various feed additives are increasingly being used. All of them must first of all be safe, and then have certain efficiency in the fight against mycotoxins. Although in vivo experiments are mandatory to assess the efficacy, in vitro test offers the advantage of rapid screening of the efficacy of a large number of food additives. In this paper, the efficiency of two commercial products belonging to the enzyme group for animal nutrition was investigated for degradation of aflatoxin B1, zearalenone and deoxynivale...
ВЕТЕРИНАРСКИ ЖУРНАЛ РЕПУБЛИКЕ СРПСКЕ, 2018
Labudovi su divlje ptice vodenih staništa i pripadaju familiji Anatidae, roduAnseriformes. S obzi... more Labudovi su divlje ptice vodenih staništa i pripadaju familiji Anatidae, roduAnseriformes. S obzirom na stanište, način života i ishranu, labudovi predstavljajuznačajnu bioindikatorsku vrstu za monitoring zagađivača u prirodi. Hrane seuglavnom vodenim biljkama i zelenim algama koje predstavljaju direktan pokazateljzagađenja životne sredine i vodenih staništa. Poznato je da se pasticidi kao i toksičnielementi akumuliraju i perzistiraju u vodenim ekosistemima i vodenim organizmima(alge, ribe, beskičmenjaci itd.) i preko lanca ishrane dospevaju i u kopnene biljke,životinje i čoveka. Cilj ovog rada je bio utvrđivanje stepena ugroženosti labudovakao zaštićene vrste, usled štetnog dejstva pesticida i toksičnih elemenata. Pripremauzoraka za određivanje pesticida je urađena u skladu sa multirezidualnom metodomQuEChERS. Ova metoda pripreme se zasniva na ekstrakciji sa acetonitrilom u prisustvusoli. Za kvantifikaciju rezidua pesticida korišćen je gasni hromatograf sa masenimdetektorom, Agilen...
Archives of Veterinary Medicine, 2021
Toxic element pollution is an ecological concern in the regions where mining, industry and agricu... more Toxic element pollution is an ecological concern in the regions where mining, industry and agriculture are developing. Anthropogenic impact on the environment results in the reduction of the population of honey bees over the world with varying degrees of morbidity and mortality. Bees exposed to contaminants produce polluted products through various sources, including foraging activities on contaminated plants. Therefore, monitoring of honey bee products in terms of toxic elements is very important for food safety and for preventing potential future ecological problems. The data presented in this review are useful for bee health protection and improving the quality and safety of honey production chain.
Archives of Veterinary Medicine, 2020
The experiment was conducted to evaluate the effect of alternative bedding materials on broiler p... more The experiment was conducted to evaluate the effect of alternative bedding materials on broiler production performances and litter quality in plant production. A 40-day experiment was carried out on 6885 broilers of ROSS 308 provenience. The broilers were reared on bedding material which consisted of cellulose pellets, wood chips, peat moss, and pH stabilizers. Feeding, zoohygienic and zootechnical measures met technological normative for this provenience. During the experiment, health status and mortality of broilers were observed. Litter pH, moisture, nitrogen, potassium and phosphorus content in litter were determined at the end of experiment. Average live weight of broilers at the end of the trial was 2620 g. Mortality of chickens was 1.52%. The results of the study indicated that use of specified bedding material was beneficial for broilers production parameters. The results of investigation of litter quality indicate that it can provide similar benefits to inorganic fertilizer...
Archives of Veterinary Medicine, 2018
In this paper, the electrical conductivity and concentration of free acids (acidity) of various t... more In this paper, the electrical conductivity and concentration of free acids (acidity) of various types of honey collected from the area of Vojvodina was investigated. Physicochemical analyzes of parameters such as electrical conductivity and acidity of honey have an important role in defining the overall properties of honey and assessing the quality of honey. A total of 55 samples of honey were examined. Out of the total number of samples, 5 samples did not meet the requirements of the Republic of Serbia Regulations on quality of honey due to inadequate electrical conductivity. Free acid values measured in tested samples ranged from 1.5 to 30 mEq / kg, and the values of electrical conductivity were in the range between 0.08 and 1.99 mS/cm. Our assumption that there is a correlation between the tested parameters has been confirmed only in meadow and polyfloral honey. Further research on the physicochemical properties of Serbian honey is recommended and important in order to establish ...
Journal of the Hellenic Veterinary Medical Society, 2018
Chromatographic methods are most commonly used for the analysis of amino acids; however, there is... more Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.0...
Archives of Veterinary Medicine, 2015
The color, aroma and fl avor are major sensory characteristics of honey, which are mainly determi... more The color, aroma and fl avor are major sensory characteristics of honey, which are mainly determined by the botanical origin of honey as well as by processing and storage conditions. Increased moisture content above the maximum permitted level could result in honey spoilage, which aff ects its sensory properties. The higher water-in-honey content, the greater possibility of yeast fermentation and thus the change of the fl avor and color ofhoney. Fermentation process results in alcohol formation and, in the presence of oxygen, the alcohol will break down to acetic acid and water, which causes honey to have sour taste. Thus, moisture content of honey is a critical parameter for its quality as it aff ects the stability of honey and its resistance to microbial spoilage during storage. Physicochemical analysis of moisture content and acidity of honey play an important role in determining the overall characteristic of honey and final assessment of its quality. In this study, the investiga...
Archives of Veterinary Medicine, 2008
Since vitamin B12 has important metabolic functions, but is unable sufficiently to synthesize int... more Since vitamin B12 has important metabolic functions, but is unable sufficiently to synthesize into organisms of domestic animals (especially non-ruminants), special attention is given to adding B12 into feed. The needs for this vitamin are provided through vitamin premixes, so determining the quantity of B12 in the samples is of utmost importance. When standard chemical methods are applied extraction and purification are required. In this paper is described a method where the content of B12 in premix with high level of different vitamins is determined through an indirect method: measuring the level of cobalt using atomic absorption spectrophotometry. The content of B12 was determined from the content of cobalt. The obtained results show that in the samples of pure B12 vitamin (used as an additive to feed) the measured values were lesser than 2% comparing to the expected values. In vitamin premixes the measured values of group B and vitamin premixes of vitamin B group and other (A, D...
Archives of Veterinary Medicine, 2008
In the period January to June 2006 the samples of feed were collected from feed factories in Sout... more In the period January to June 2006 the samples of feed were collected from feed factories in Southern Baåka and Srem district. The samples of raw milk and full mix were taken from 5 dairy farms. A total of 50 raw milk samples was examined. The samples were examined on the presence of aflatoxin B1 using the method of thin layer chromatography (TLC) and simultaneously, using ELISA tests. Milk samples were examined using immunoenzyme tests for the presence of aflatoxin M1. Aflatoxin content in all the examined feed and mix samples was below LOD (limit of detection) of TLC method, also this content was below MRL according to ELISA method. In total of 50 samples of raw milk, aflatoxin M1 was detected in two samples originating from different farms. Aflatoxin was detected in 7.5 ng/l, i.e. 10 ng/l respectively, what is considerably lower than MRL. Based on the obtained results it is considered that obligatory control of raw milk for the presence of aflatoxin is necessary.
Archives of Veterinary Medicine, 2012
The main food preservatives added to meat products are nitrites and nitrates, and the most import... more The main food preservatives added to meat products are nitrites and nitrates, and the most important antioxidants are phosphates. The content of additives in meat products is prescribed by the Rule Book. The aim of this paper was to monitor the content of nitrites and total phosphorus in meat products of producers from Novi Sad market, and, based on these results, find out whether the additives are used correctly. In one year period the content of additives in the products of four producers that sell on Novi Sad market was monitored. A total of 140 samples was examined. Phosphorus content was highly variable and ranged from 2.34 g/kg-8.45 g/kg. The nitrite level was the highest in the coarse-grained cooked sausages, but was not significantly different from the products of other producers. Only in two samples (1.43%) phosphorus content was higher than the prescribed value, and only in one tested sample it was outside the permissible value (0.71%). It can be concluded that the produce...
Archives of Veterinary Medicine, 2016
Validation of analytical methods ensures the reliability and accuracy of analytical results. To g... more Validation of analytical methods ensures the reliability and accuracy of analytical results. To get a reliable result we performed a validation ofthe method taking into account all factors that may aff ect of the result. In this paper we optimized QuEChERS method for food and feed sample preparation as well as instrumental method using gas chromatography with mass spectrometry (GC-MS), and we obtained a method for successful determination of pesticides with calibration through the matrix. In this way, applying calibration through matrix, we satisfi ed the requirements for precision and reproducibility of the method being less than 20%, the accuracy rate in the range of 70-130% and method linearity throughout the range of interest.
Archives of Veterinary Medicine, 2013
Table salt is the first food additive and its preserving eff ects are well known since long time ... more Table salt is the first food additive and its preserving eff ects are well known since long time ago. Because of its role in everyday diet, table salt is one of the fi rst products designated as “functional food”. However, it was established that excessive salt intake, i.e. sodium intake, is frequently associated with hypertension and development of a range of other diseases. Sodium chloride affects sensory and microbiological properties of meat products; however, it aff ects the human health as well. Since the current Regulation (Offi cial Gazette of the Republic of Serbia No. 31/2012) did not define the sodium chloride content of in meat products as a quality parameter, the aim of our study was to determine levels of sodium chloride in diverse meat products available at the market in Novi Sad. A total of 260 samples of meat products were examined, including: shaped ground (minced) meat, vegetarian spreads, smoked products, fermented dry sausages, fi nely minced boiled sausages, ca...
Archives of Veterinary Medicine, 2019
One hundred thirty-three honey samples of different floral origin from two different production y... more One hundred thirty-three honey samples of different floral origin from two different production years (2017 and 2018) from Serbia were examined in order to determine water content. The water content in honey affects physical, microbiological, sensory properties, and commercial value of honey. The water content in all examined honey samples produced in 2017 (56) was below maximum permissible level set by local regulations for honey. Out of the total of 77 tested honey samples that were produced in 2018, 3 samples (3.9%) did not comply with the provisions of the Regulation. By analysing honey samples originating from 2017, there was a significant difference between the water contents betwen linden honey and honeydew (p = 0.0027). The same result was obtained based on the water content in different honey types from the year 2018 (p = 0.00022). Using the F test, it has been shown that there is no significant difference in the water content between certain types of honey produced in thes...
Archives of Veterinary Medicine, 2019
Vanadium (V) is essential element for poultry nutrition. Relatively low level of V (< 10 μg/kg... more Vanadium (V) is essential element for poultry nutrition. Relatively low level of V (< 10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples ...
Food and Feed Research, 2018
The health safety of honey includes the correctness in terms of the presence of different contami... more The health safety of honey includes the correctness in terms of the presence of different contaminants, which also implies the antibiotic residue. The presence of antibiotics in honey is prohibited, and methods of food analysis are prescribed in order to reliably determine antibiotics in food. In this paper the application of ELISA tests for determination of selected antibiotics and sulfonamides in honey is shown. The possibility of using four ELISA tests for the analysis of tetracyclines, streptomycines, chloramphenicol and sulfonamides was examined. Each test was evaluated after application on honey samples spiked with standard solution of a particular analyte. Samples were prepared according to the instructions of the ELISA test manufacturer referring to honey. Results of investigation of all ELISA tests, except for sulphonamides, have shown satisfactory accuracy (73-111%) and precision (14-16%). Recovery for sulfametoxypyridazin was low (40%), and for low tetracycline concentration was somewhat higher than acceptable (139%). The detection limits were in accordance with the limits given by the ELISA kit manufacturer and are also satisfactory in relation to the requirements of the legislation (0.075-3 µg/kg). The test kits can be used to screen the presence of tetracycline, chloramphenicol and streptomycin in the honey, taking into account the obtained validation parameters.
RAD Conference Proceedings, 2019
The naturally occurring radionuclides are the major source of radiation to which humans are expos... more The naturally occurring radionuclides are the major source of radiation to which humans are exposed to. A total of 21 samples of roe deer (Capreolus capreolus) from different locations in Vojvodina were tested. The samples were prepared for measuring by microwave digestion. The content of potassium in the samples was determined using atomic absorption spectrometry (AAS) The total content of uranium and thorium were determined using inductively coupled plasma mass spectrometry (ICP-MS). The concentration of the activity of the tested radionuclides was calculated based on their specific activities. It can also be concluded that potassium-40 is the predominant natural radionuclide in urine as compared to other radionuclides and it moved in the range of 128-381 Bq/L. The results of urine analysis showed wide ranges in radionuclide activity concentrations: 18.4-231.9 mBq/L for 238 U, 0.94-11.90 mBq/L for 235 U and 1.03-89.02 mBq/L for 232 Th.
Sustainability
In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater ... more In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater from the slaughterhouse, and in this way integrates a new value chain in the form of purification and use of slaughterhouse wastewater in an aquaculture production system. The negative aspect of such integrated production systems is the concern related to the safety of fish meat produced in these systems. The aim of this research was to determine the activity level of Cs-137, K-40, Ra-226, Th-232, and Na-22 in water, sediment, and carp harvested from a pond that received purified water from a slaughterhouse. All samples were collected in spring and in autumn. The activity concentrations of selected radionuclides were determined by gamma spectrometry (HPG-e detector). The activity of K-40 in water samples ranged from 8.4 to 15.6 Bq L−1. The specific activity concentrations of the Ra-226 in water samples ranged between 1.02 ± 0.11 and 2.76 ± 0.49 Bq kg−1. The results of the activity of nat...
Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg ... more Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples w...
Sustainability
In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater ... more In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater from the slaughterhouse, and in this way integrates a new value chain in the form of purification and use of slaughterhouse wastewater in an aquaculture production system. The negative aspect of such integrated production systems is the concern related to the safety of fish meat produced in these systems. The aim of this research was to determine the activity level of Cs-137, K-40, Ra-226, Th-232, and Na-22 in water, sediment, and carp harvested from a pond that received purified water from a slaughterhouse. All samples were collected in spring and in autumn. The activity concentrations of selected radionuclides were determined by gamma spectrometry (HPG-e detector). The activity of K-40 in water samples ranged from 8.4 to 15.6 Bq L−1. The specific activity concentrations of the Ra-226 in water samples ranged between 1.02 ± 0.11 and 2.76 ± 0.49 Bq kg−1. The results of the activity of nat...
Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg ... more Vanadium (V) is essential element for poultry nutrition. Relatively low level of V ( lt 10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples w...
Archives of Veterinary Medicine
Providing healthy and safe food in terms of mycotoxicological safety is an imperative for not onl... more Providing healthy and safe food in terms of mycotoxicological safety is an imperative for not only good and sustainable livestock production, but also for the population that consumes food of both plant and animal origin. Climate change in the temperate regions of southern Europe has led to frequent occurrence of aflatoxins, deoxynivalenol and zearalenone in cereals. In order to reduce harmful effects of these toxins on animal health as well as to avoid large economic losses, various feed additives are increasingly being used. All of them must first of all be safe, and then have certain efficiency in the fight against mycotoxins. Although in vivo experiments are mandatory to assess the efficacy, in vitro test offers the advantage of rapid screening of the efficacy of a large number of food additives. In this paper, the efficiency of two commercial products belonging to the enzyme group for animal nutrition was investigated for degradation of aflatoxin B1, zearalenone and deoxynivale...
ВЕТЕРИНАРСКИ ЖУРНАЛ РЕПУБЛИКЕ СРПСКЕ, 2018
Labudovi su divlje ptice vodenih staništa i pripadaju familiji Anatidae, roduAnseriformes. S obzi... more Labudovi su divlje ptice vodenih staništa i pripadaju familiji Anatidae, roduAnseriformes. S obzirom na stanište, način života i ishranu, labudovi predstavljajuznačajnu bioindikatorsku vrstu za monitoring zagađivača u prirodi. Hrane seuglavnom vodenim biljkama i zelenim algama koje predstavljaju direktan pokazateljzagađenja životne sredine i vodenih staništa. Poznato je da se pasticidi kao i toksičnielementi akumuliraju i perzistiraju u vodenim ekosistemima i vodenim organizmima(alge, ribe, beskičmenjaci itd.) i preko lanca ishrane dospevaju i u kopnene biljke,životinje i čoveka. Cilj ovog rada je bio utvrđivanje stepena ugroženosti labudovakao zaštićene vrste, usled štetnog dejstva pesticida i toksičnih elemenata. Pripremauzoraka za određivanje pesticida je urađena u skladu sa multirezidualnom metodomQuEChERS. Ova metoda pripreme se zasniva na ekstrakciji sa acetonitrilom u prisustvusoli. Za kvantifikaciju rezidua pesticida korišćen je gasni hromatograf sa masenimdetektorom, Agilen...
Archives of Veterinary Medicine, 2021
Toxic element pollution is an ecological concern in the regions where mining, industry and agricu... more Toxic element pollution is an ecological concern in the regions where mining, industry and agriculture are developing. Anthropogenic impact on the environment results in the reduction of the population of honey bees over the world with varying degrees of morbidity and mortality. Bees exposed to contaminants produce polluted products through various sources, including foraging activities on contaminated plants. Therefore, monitoring of honey bee products in terms of toxic elements is very important for food safety and for preventing potential future ecological problems. The data presented in this review are useful for bee health protection and improving the quality and safety of honey production chain.
Archives of Veterinary Medicine, 2020
The experiment was conducted to evaluate the effect of alternative bedding materials on broiler p... more The experiment was conducted to evaluate the effect of alternative bedding materials on broiler production performances and litter quality in plant production. A 40-day experiment was carried out on 6885 broilers of ROSS 308 provenience. The broilers were reared on bedding material which consisted of cellulose pellets, wood chips, peat moss, and pH stabilizers. Feeding, zoohygienic and zootechnical measures met technological normative for this provenience. During the experiment, health status and mortality of broilers were observed. Litter pH, moisture, nitrogen, potassium and phosphorus content in litter were determined at the end of experiment. Average live weight of broilers at the end of the trial was 2620 g. Mortality of chickens was 1.52%. The results of the study indicated that use of specified bedding material was beneficial for broilers production parameters. The results of investigation of litter quality indicate that it can provide similar benefits to inorganic fertilizer...
Archives of Veterinary Medicine, 2018
In this paper, the electrical conductivity and concentration of free acids (acidity) of various t... more In this paper, the electrical conductivity and concentration of free acids (acidity) of various types of honey collected from the area of Vojvodina was investigated. Physicochemical analyzes of parameters such as electrical conductivity and acidity of honey have an important role in defining the overall properties of honey and assessing the quality of honey. A total of 55 samples of honey were examined. Out of the total number of samples, 5 samples did not meet the requirements of the Republic of Serbia Regulations on quality of honey due to inadequate electrical conductivity. Free acid values measured in tested samples ranged from 1.5 to 30 mEq / kg, and the values of electrical conductivity were in the range between 0.08 and 1.99 mS/cm. Our assumption that there is a correlation between the tested parameters has been confirmed only in meadow and polyfloral honey. Further research on the physicochemical properties of Serbian honey is recommended and important in order to establish ...
Journal of the Hellenic Veterinary Medical Society, 2018
Chromatographic methods are most commonly used for the analysis of amino acids; however, there is... more Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.0...
Archives of Veterinary Medicine, 2015
The color, aroma and fl avor are major sensory characteristics of honey, which are mainly determi... more The color, aroma and fl avor are major sensory characteristics of honey, which are mainly determined by the botanical origin of honey as well as by processing and storage conditions. Increased moisture content above the maximum permitted level could result in honey spoilage, which aff ects its sensory properties. The higher water-in-honey content, the greater possibility of yeast fermentation and thus the change of the fl avor and color ofhoney. Fermentation process results in alcohol formation and, in the presence of oxygen, the alcohol will break down to acetic acid and water, which causes honey to have sour taste. Thus, moisture content of honey is a critical parameter for its quality as it aff ects the stability of honey and its resistance to microbial spoilage during storage. Physicochemical analysis of moisture content and acidity of honey play an important role in determining the overall characteristic of honey and final assessment of its quality. In this study, the investiga...
Archives of Veterinary Medicine, 2008
Since vitamin B12 has important metabolic functions, but is unable sufficiently to synthesize int... more Since vitamin B12 has important metabolic functions, but is unable sufficiently to synthesize into organisms of domestic animals (especially non-ruminants), special attention is given to adding B12 into feed. The needs for this vitamin are provided through vitamin premixes, so determining the quantity of B12 in the samples is of utmost importance. When standard chemical methods are applied extraction and purification are required. In this paper is described a method where the content of B12 in premix with high level of different vitamins is determined through an indirect method: measuring the level of cobalt using atomic absorption spectrophotometry. The content of B12 was determined from the content of cobalt. The obtained results show that in the samples of pure B12 vitamin (used as an additive to feed) the measured values were lesser than 2% comparing to the expected values. In vitamin premixes the measured values of group B and vitamin premixes of vitamin B group and other (A, D...
Archives of Veterinary Medicine, 2008
In the period January to June 2006 the samples of feed were collected from feed factories in Sout... more In the period January to June 2006 the samples of feed were collected from feed factories in Southern Baåka and Srem district. The samples of raw milk and full mix were taken from 5 dairy farms. A total of 50 raw milk samples was examined. The samples were examined on the presence of aflatoxin B1 using the method of thin layer chromatography (TLC) and simultaneously, using ELISA tests. Milk samples were examined using immunoenzyme tests for the presence of aflatoxin M1. Aflatoxin content in all the examined feed and mix samples was below LOD (limit of detection) of TLC method, also this content was below MRL according to ELISA method. In total of 50 samples of raw milk, aflatoxin M1 was detected in two samples originating from different farms. Aflatoxin was detected in 7.5 ng/l, i.e. 10 ng/l respectively, what is considerably lower than MRL. Based on the obtained results it is considered that obligatory control of raw milk for the presence of aflatoxin is necessary.
Archives of Veterinary Medicine, 2012
The main food preservatives added to meat products are nitrites and nitrates, and the most import... more The main food preservatives added to meat products are nitrites and nitrates, and the most important antioxidants are phosphates. The content of additives in meat products is prescribed by the Rule Book. The aim of this paper was to monitor the content of nitrites and total phosphorus in meat products of producers from Novi Sad market, and, based on these results, find out whether the additives are used correctly. In one year period the content of additives in the products of four producers that sell on Novi Sad market was monitored. A total of 140 samples was examined. Phosphorus content was highly variable and ranged from 2.34 g/kg-8.45 g/kg. The nitrite level was the highest in the coarse-grained cooked sausages, but was not significantly different from the products of other producers. Only in two samples (1.43%) phosphorus content was higher than the prescribed value, and only in one tested sample it was outside the permissible value (0.71%). It can be concluded that the produce...
Archives of Veterinary Medicine, 2016
Validation of analytical methods ensures the reliability and accuracy of analytical results. To g... more Validation of analytical methods ensures the reliability and accuracy of analytical results. To get a reliable result we performed a validation ofthe method taking into account all factors that may aff ect of the result. In this paper we optimized QuEChERS method for food and feed sample preparation as well as instrumental method using gas chromatography with mass spectrometry (GC-MS), and we obtained a method for successful determination of pesticides with calibration through the matrix. In this way, applying calibration through matrix, we satisfi ed the requirements for precision and reproducibility of the method being less than 20%, the accuracy rate in the range of 70-130% and method linearity throughout the range of interest.
Archives of Veterinary Medicine, 2013
Table salt is the first food additive and its preserving eff ects are well known since long time ... more Table salt is the first food additive and its preserving eff ects are well known since long time ago. Because of its role in everyday diet, table salt is one of the fi rst products designated as “functional food”. However, it was established that excessive salt intake, i.e. sodium intake, is frequently associated with hypertension and development of a range of other diseases. Sodium chloride affects sensory and microbiological properties of meat products; however, it aff ects the human health as well. Since the current Regulation (Offi cial Gazette of the Republic of Serbia No. 31/2012) did not define the sodium chloride content of in meat products as a quality parameter, the aim of our study was to determine levels of sodium chloride in diverse meat products available at the market in Novi Sad. A total of 260 samples of meat products were examined, including: shaped ground (minced) meat, vegetarian spreads, smoked products, fermented dry sausages, fi nely minced boiled sausages, ca...
Archives of Veterinary Medicine, 2019
One hundred thirty-three honey samples of different floral origin from two different production y... more One hundred thirty-three honey samples of different floral origin from two different production years (2017 and 2018) from Serbia were examined in order to determine water content. The water content in honey affects physical, microbiological, sensory properties, and commercial value of honey. The water content in all examined honey samples produced in 2017 (56) was below maximum permissible level set by local regulations for honey. Out of the total of 77 tested honey samples that were produced in 2018, 3 samples (3.9%) did not comply with the provisions of the Regulation. By analysing honey samples originating from 2017, there was a significant difference between the water contents betwen linden honey and honeydew (p = 0.0027). The same result was obtained based on the water content in different honey types from the year 2018 (p = 0.00022). Using the F test, it has been shown that there is no significant difference in the water content between certain types of honey produced in thes...
Archives of Veterinary Medicine, 2019
Vanadium (V) is essential element for poultry nutrition. Relatively low level of V (< 10 μg/kg... more Vanadium (V) is essential element for poultry nutrition. Relatively low level of V (< 10 μg/kg of feed) is known to reduce both growth in chicks and Haugh unit value of eggs. The National Research Council (NRC) recommends the presence of very low levels of V in poultry diets, with the maximum tolerance level (MTL) being 10 mg/kg. Excessive vanadium in poultry diets has been shown to be detrimental to egg production, interior quality of eggs (albumen height), body weight and feed consumption. There is little information on the content of V in feedstuffs. Phosphates are known to be the cause of excessive V in various types of poultry diets. The objective of this study was to obtain information about the content of vanadium in phosphates and poultry feed. The samples were prepared by microwave wet digestion. Content of V was determined by the method of coupled plasma with mass spectrometry on the Agilent ICP-MS 7700. The concentrations of vanadium determined in the examined samples ...
Food and Feed Research, 2018
The health safety of honey includes the correctness in terms of the presence of different contami... more The health safety of honey includes the correctness in terms of the presence of different contaminants, which also implies the antibiotic residue. The presence of antibiotics in honey is prohibited, and methods of food analysis are prescribed in order to reliably determine antibiotics in food. In this paper the application of ELISA tests for determination of selected antibiotics and sulfonamides in honey is shown. The possibility of using four ELISA tests for the analysis of tetracyclines, streptomycines, chloramphenicol and sulfonamides was examined. Each test was evaluated after application on honey samples spiked with standard solution of a particular analyte. Samples were prepared according to the instructions of the ELISA test manufacturer referring to honey. Results of investigation of all ELISA tests, except for sulphonamides, have shown satisfactory accuracy (73-111%) and precision (14-16%). Recovery for sulfametoxypyridazin was low (40%), and for low tetracycline concentration was somewhat higher than acceptable (139%). The detection limits were in accordance with the limits given by the ELISA kit manufacturer and are also satisfactory in relation to the requirements of the legislation (0.075-3 µg/kg). The test kits can be used to screen the presence of tetracycline, chloramphenicol and streptomycin in the honey, taking into account the obtained validation parameters.
RAD Conference Proceedings, 2019
The naturally occurring radionuclides are the major source of radiation to which humans are expos... more The naturally occurring radionuclides are the major source of radiation to which humans are exposed to. A total of 21 samples of roe deer (Capreolus capreolus) from different locations in Vojvodina were tested. The samples were prepared for measuring by microwave digestion. The content of potassium in the samples was determined using atomic absorption spectrometry (AAS) The total content of uranium and thorium were determined using inductively coupled plasma mass spectrometry (ICP-MS). The concentration of the activity of the tested radionuclides was calculated based on their specific activities. It can also be concluded that potassium-40 is the predominant natural radionuclide in urine as compared to other radionuclides and it moved in the range of 128-381 Bq/L. The results of urine analysis showed wide ranges in radionuclide activity concentrations: 18.4-231.9 mBq/L for 238 U, 0.94-11.90 mBq/L for 235 U and 1.03-89.02 mBq/L for 232 Th.