ADENIYI DANIEL - Academia.edu (original) (raw)

Papers by ADENIYI DANIEL

Research paper thumbnail of Lead induces inflammation and neurodegenerative changes in the rat medial prefrontal cortex

Anatomy

Objectives: Lead (Pb) is a neurotoxicant heavy metal ubiquitously present in the ecosystem. The p... more Objectives: Lead (Pb) is a neurotoxicant heavy metal ubiquitously present in the ecosystem. The precise mechanism by which Pb confers its deleterious effects on the cellular profile of the central nervous system remains unknown. The aim of this study was to investigate the effect of Pb on the medial prefrontal cortex (mPFC) using histological, immunohistological and morphological techniques. Methods: Thirthy juvenile male Wistar rats were used in this study. The rats were randomly assigned into three groups. Group A served as the control group, Group B received 5 mg/kg Pb-nitrate (PbNO3) orally for 21 days, and Group C received 5 mg/kg PbNO3 and left for an additional 21 days to recover. Results: There was a significant decrease in the number of normal neurons in the mPFC of the PbNO3-treated rats. The number of degenerating neurons significantly increased in the PbNO3-treated groups compared with the control group. A marked increase was observed in the number of astrocytic cell count in the PbNO3-treated groups compared with the control. The neuronal cells in the cytoarchitectural profile of the mPFC of the rats receiving PbNO3 showed marked neurodegenerative modification with features of distorted morphology, swollen and vacuolized cytoplasm, and features of either pyknotic or karyorrhectic nuclei. The cytoarchitecture of the mPFC of the rats in the control group preserved the normal histological outline suggestive of a normal and functional mPFC. Conclusion: Exposure to Pb ingestion can result in significant inflammatory responses in the cytoarchitectural profile of the mPFC. Furthermore, 21 days of cessation of exposure to PbNO3 did not halt or reverse the deleterious effects of Pb on the mPFC of the rats, suggesting that Pb persists in the central nervous system of the rats.

Research paper thumbnail of The promise of computational journalism

Computational journalism involves the application of software and technologies to the activities ... more Computational journalism involves the application of software and technologies to the activities of journalism, and it draws from the fields of computer science, the social sciences, and media and communications. New technologies may enhance the traditional aims of journalism, or may initiate greater interaction between journalists and information and communication technology (ICT) specialists. The enhanced use of computing in news production is related in particular to three factors: larger government datasets becoming more widely available; the increasingly sophisticated and ubiquitous nature of software; and the developing digital economy. Drawing upon international examples, this paper argues that computational journalism techniques may provide new foundations for original investigative journalism and increase the scope for new forms of interaction with readers. Computational journalism provides a major opportunity to enhance the production of original investigative journalism, and to attract and retain readers online.

Research paper thumbnail of Evolution of structural and optical properties of rutile TiO 2 thin films synthesized at room temperature by chemical bath deposition method

We have investigated the evolution of structural and optical properties of GaAsN nanostructures s... more We have investigated the evolution of structural and optical properties of GaAsN nanostructures synthesized by N ion implantation into epitaxial GaAs, followed by rapid thermal annealing. Transmission electron microscopy and x-ray diffraction indicate the formation of nanometer-sized crystallites with lattice parameters close to those of pure zincblende GaN. The average crystallite size increases with annealing temperature while the size distribution is self-similar and the volume fraction remains constant, suggesting a coarsening process governed by Ostwald ripening. These GaAsN nanostructures exhibit significant photoluminescence in the near infrared range. The apparent lowering of the fundamental band gap is likely due to the incorporation of a small amount of As in GaN.

Research paper thumbnail of Structural Investigations on Self‐Organized Si/SiGe Islands by Grazing Incidence Small Angle X‐Ray Scattering

… status solidi (b), 2001

... We present a novel technique, based on grazing incidence small angle X-ray scattering (GISAXS... more ... We present a novel technique, based on grazing incidence small angle X-ray scattering (GISAXS), with which information on the shape and the lateral correlation of buried islands can be obtained. ... The GISAXS data show that the islands change their shape after annealing. ...

Research paper thumbnail of Strain and composition distribution in uncapped SiGe islands from x-ray diffraction

Applied Physics …, 2001

... Repeating the procedure while varying the Ge distribution and the island shape until a good c... more ... Repeating the procedure while varying the Ge distribution and the island shape until a good correspondence between experiment and simulation is reached, we are able to establish the distributions of Ge content and strain within the islands. ...

Research paper thumbnail of Preserved Sm-Nd Isotopic Composition as Useful Provenance Indicators in Neoproterozoic Sandstones in the Voltaian Basin, Ghana

The provenance of sandstones derived from the Lower Voltaian Kwahu-Morago Group and the Middle Vo... more The provenance of sandstones derived from the Lower Voltaian Kwahu-Morago Group and the Middle Voltaian Oti-Pendjari Group of the Neoproterozoic Voltaian Basin are discriminated by their Sm-Nd Isotopic compositions. Plots from the Sm-Nd data suggested provenance of the Kwahu-Morago Group to be from the Birimian metasediments and associated "basin type" granitoids. The Sm-Nd studies have further revealed an average T DM model age of whole rock samples in the Kwahu-Morago Group to be 2.2 Ga which shows that this portion of the Voltaian Supergroup represents eroded remnants of "basin type" granitoids. Sm-Nd data from the Oti-Pendjari Group suggested provenance from the Birimian volcanic rocks and probably with contribution from the Pan African rocks. Its average T DM model age of whole rock samples was 2.0 Ga, which generally falls in the range of the model ages for the basement Birimian volcanic rocks as well as the model ages for the granitoid rocks and thus suggests the major source rock of the Oti-Pendjari Group as coming from the volcanic belts. The model ages for both groups seem to indicate clastic supply from an early Proterozoic crustal provenance. This study shows that whole rock isotopic analyses can also be complementary in providing an insight into the origin and development of sedimentary successions.

Research paper thumbnail of Partial wave analysis of the reaction γp→ pω and the search for nucleon resonances

An event-based partial wave analysis (PWA) of the reaction γp → pω has been performed on a high-s... more An event-based partial wave analysis (PWA) of the reaction γp → pω has been performed on a high-statistics dataset obtained using the CLAS at Jefferson Lab for center-of-mass energies from threshold up to 2.4 GeV. This analysis benefits from access to the world's first high-precision spin density matrix element measurements, available to the event-based PWA through the decay distribution of ω → π + π − π 0. The data confirm the dominance of the t-channel π 0 exchange amplitude in the forward direction. The dominant resonance contributions are consistent with the previously identified states F15(1680) and D13(1700) near threshold, as well as the G17(2190) at higher energies. Suggestive evidence for the presence of a J P = 5/2 + state around 2 GeV, a "missing" state, has also been found. Evidence for other states is inconclusive.

Research paper thumbnail of Characterizing canopy gap dynamics using hemispherical photography in emerald ash borer-impacted forests

ABSTRACT Background/Question/Methods Ash (Fraxinus species) mortality due to emerald ash borer (E... more ABSTRACT Background/Question/Methods Ash (Fraxinus species) mortality due to emerald ash borer (EAB; Agrilus planipennis) infestation may drastically alter the structure and composition of native forests. Canopy gaps resulting from dead and fallen trees can impact understory microclimates and ecological interactions. This in turn may result in altered successional trajectories, and potentially facilitate the establishment and growth of invasive plant species. From 2008 through 2010 we have taken hemispherical photographs at 5 to 20 points per plot in 129 plots within 7 different Michigan State Parks or Metroparks. Our objectives were to characterize variation among points within a plot, plots within a transect, transects within a park, among parks, and over time. We also compared canopy gap fraction with ash mortality, and understory plant growth, abundance, and composition over time. Results/Conclusions Gap fraction for a subset of plots in 2009 ranged from 0.5 to 39.3%. Photographs taken in 2010 had a median gap fraction of 6.73%; this is a slight decline from the plots in 2009, which had a median of 10.0%. Apparent decrease in median % gap fraction from 2009 to 2010 indicates possible initial stages of canopy closure as trees grow up or outward to fill in the gaps. However, initial closure may be reversed as standing dead ash trees fall, potentially creating even larger canopy gaps. Within-plot variation, analyzed for a subset of plots sampled in 2010, showed a minimum variation of 3.0% gap fraction, while the maximum was 9.4%. The median values among plots ranged from 3.7% to 9.1% gap fraction. Within-transect variation was even smaller, with a maximum variation of 5.0% A preliminary analysis of variance on a subset of the data attributed the majority of variation (75%) to differences among photographs within plots.

Research paper thumbnail of The response of birds to abrupt natural hazards as observed using weather radar

Research paper thumbnail of Absorption of the ω and ϕ Mesons in Nuclei

Physical Review Letters, 2010

Due to their long lifetimes, the ω and φ mesons are the ideal candidates for the study of possibl... more Due to their long lifetimes, the ω and φ mesons are the ideal candidates for the study of possible modifications of the in-medium meson-nucleon interaction through their absorption inside the nucleus. During the E01-112 experiment at the Thomas Jefferson National Accelerator Facility, the mesons were photoproduced from 2 H, C, Ti, Fe, and Pb targets. This paper reports the first measurement of the ratio of nuclear transparencies for the e + e − channel. The ratios indicate larger in-medium widths compared with what have been reported in other reaction channels.

Research paper thumbnail of Observation of the Helium 7 Lambda hypernucleus by the (e, e'K+) reaction

An experiment with a newly developed high-resolution kaon spectrometer (HKS) and a scattered elec... more An experiment with a newly developed high-resolution kaon spectrometer (HKS) and a scattered electron spectrometer with a novel configuration was performed in Hall C at Jefferson Lab (JLab). The ground state of a neutron-rich hypernucleus, 7 Λ He, was observed for the first time with the (e, e ′ K +) reaction with an energy resolution of ∼0.6 MeV. This resolution is the best reported to date for hypernuclear reaction spectroscopy. The 7 Λ He binding energy supplies the last missing information of the A = 7, T = 1 hypernuclear iso-triplet, providing a new input for the charge symmetry breaking (CSB) effect of ΛN potential.

Research paper thumbnail of Microstructural Evolution and Optical Properties of (InGa)(AsN) Nanostructures Synthesized by Ion Implantation

Mixed anion nitride-arsenide compound semiconductor heterostructures are promising for light-emit... more Mixed anion nitride-arsenide compound semiconductor heterostructures are promising for light-emitting devices operating throughout the near infrared range. However, due to the large N-As size difference, a limited miscibility of (InGa)(AsN) on the anion sublattice is predicted. Furthermore, alloy phase separation resulting in the formation of quantum dot-like nanostructures has been reported in GaAsN/GaAs [1] and InGaAsN/GaAs [2] superlattices. We have

Research paper thumbnail of Does a nucleon appears different when inside a nucleus?

Research paper thumbnail of Measurements of the EMC effect in few-body nuclei at large x

Recent data on the EMC effect from Jefferson Lab experiment E03103 suggest that the nuclear depen... more Recent data on the EMC effect from Jefferson Lab experiment E03103 suggest that the nuclear dependence of the high x quark distribution may depend on the local nuclear environment, rather than being a purely bulk effect. We briefly discuss the analysis and results of the E03103 experiment focusing on the light nuclei and high x region. In addition, we also discuss a future experiment at the upgraded 12 GeV Jefferson Lab facility which will further investigate the role of the local nuclear environment and the influence of detailed nuclear structure on the modification of quark distributions.

Research paper thumbnail of A flow cell for on-line monitoring of metals in natural waters by voltammetry with a mercury drop electrode

Analytica Chimica Acta, 1997

A cell was developed to enable flow analysis with voltammetric detection using a hanging mercury ... more A cell was developed to enable flow analysis with voltammetric detection using a hanging mercury drop electrode (HMDE). The flow cell has a bore of 0.7 mm in which the mercury drop is centred, and has ports for a reference and a counter electrode, and for the capillary of the ...

Research paper thumbnail of Rapid DNA library construction for functional genomic and metagenomic screening

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomi... more A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/ metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

Research paper thumbnail of The Poetry of Arnaut Daniel

Research paper thumbnail of Identification of marker chromosomes in thirteen patients using FISH probing

American Journal of Medical Genetics, 1994

Research paper thumbnail of Evaluating the activity of the RNA polymerase inhibitor myxopyronin B against Staphylococcus aureus

Myxopyronin B (MyxB) binds to the switch region of RNA polymerase (RNAP) and inhibits transcripti... more Myxopyronin B (MyxB) binds to the switch region of RNA polymerase (RNAP) and inhibits transcriptional initiation. To evaluate the potential development of MyxB as a novel class of antibiotic, we characterized the antimicrobial activity of MyxB against Staphylococcus aureus. Spontaneous MyxB resistance in S. aureus occurred at a frequency of 8 Â 10 À8 , similar to that of rifampin. The MyxBresistant mutants were found to be altered in single amino acid residues in the RNAP subunits that form the MyxB-binding site. In the presence of human serum albumin, the MyxB minimum inhibitory concentration against S. aureus increased drastically (Z128-fold) and 99.5% of MyxB was protein bound. Because of the high serum protein binding and resistance rate, we conclude that MyxB is not a viable starting point for antibiotic development.

Research paper thumbnail of Measurement of the nuclear multiplicity ratio for< span>< img height

Research paper thumbnail of Lead induces inflammation and neurodegenerative changes in the rat medial prefrontal cortex

Anatomy

Objectives: Lead (Pb) is a neurotoxicant heavy metal ubiquitously present in the ecosystem. The p... more Objectives: Lead (Pb) is a neurotoxicant heavy metal ubiquitously present in the ecosystem. The precise mechanism by which Pb confers its deleterious effects on the cellular profile of the central nervous system remains unknown. The aim of this study was to investigate the effect of Pb on the medial prefrontal cortex (mPFC) using histological, immunohistological and morphological techniques. Methods: Thirthy juvenile male Wistar rats were used in this study. The rats were randomly assigned into three groups. Group A served as the control group, Group B received 5 mg/kg Pb-nitrate (PbNO3) orally for 21 days, and Group C received 5 mg/kg PbNO3 and left for an additional 21 days to recover. Results: There was a significant decrease in the number of normal neurons in the mPFC of the PbNO3-treated rats. The number of degenerating neurons significantly increased in the PbNO3-treated groups compared with the control group. A marked increase was observed in the number of astrocytic cell count in the PbNO3-treated groups compared with the control. The neuronal cells in the cytoarchitectural profile of the mPFC of the rats receiving PbNO3 showed marked neurodegenerative modification with features of distorted morphology, swollen and vacuolized cytoplasm, and features of either pyknotic or karyorrhectic nuclei. The cytoarchitecture of the mPFC of the rats in the control group preserved the normal histological outline suggestive of a normal and functional mPFC. Conclusion: Exposure to Pb ingestion can result in significant inflammatory responses in the cytoarchitectural profile of the mPFC. Furthermore, 21 days of cessation of exposure to PbNO3 did not halt or reverse the deleterious effects of Pb on the mPFC of the rats, suggesting that Pb persists in the central nervous system of the rats.

Research paper thumbnail of The promise of computational journalism

Computational journalism involves the application of software and technologies to the activities ... more Computational journalism involves the application of software and technologies to the activities of journalism, and it draws from the fields of computer science, the social sciences, and media and communications. New technologies may enhance the traditional aims of journalism, or may initiate greater interaction between journalists and information and communication technology (ICT) specialists. The enhanced use of computing in news production is related in particular to three factors: larger government datasets becoming more widely available; the increasingly sophisticated and ubiquitous nature of software; and the developing digital economy. Drawing upon international examples, this paper argues that computational journalism techniques may provide new foundations for original investigative journalism and increase the scope for new forms of interaction with readers. Computational journalism provides a major opportunity to enhance the production of original investigative journalism, and to attract and retain readers online.

Research paper thumbnail of Evolution of structural and optical properties of rutile TiO 2 thin films synthesized at room temperature by chemical bath deposition method

We have investigated the evolution of structural and optical properties of GaAsN nanostructures s... more We have investigated the evolution of structural and optical properties of GaAsN nanostructures synthesized by N ion implantation into epitaxial GaAs, followed by rapid thermal annealing. Transmission electron microscopy and x-ray diffraction indicate the formation of nanometer-sized crystallites with lattice parameters close to those of pure zincblende GaN. The average crystallite size increases with annealing temperature while the size distribution is self-similar and the volume fraction remains constant, suggesting a coarsening process governed by Ostwald ripening. These GaAsN nanostructures exhibit significant photoluminescence in the near infrared range. The apparent lowering of the fundamental band gap is likely due to the incorporation of a small amount of As in GaN.

Research paper thumbnail of Structural Investigations on Self‐Organized Si/SiGe Islands by Grazing Incidence Small Angle X‐Ray Scattering

… status solidi (b), 2001

... We present a novel technique, based on grazing incidence small angle X-ray scattering (GISAXS... more ... We present a novel technique, based on grazing incidence small angle X-ray scattering (GISAXS), with which information on the shape and the lateral correlation of buried islands can be obtained. ... The GISAXS data show that the islands change their shape after annealing. ...

Research paper thumbnail of Strain and composition distribution in uncapped SiGe islands from x-ray diffraction

Applied Physics …, 2001

... Repeating the procedure while varying the Ge distribution and the island shape until a good c... more ... Repeating the procedure while varying the Ge distribution and the island shape until a good correspondence between experiment and simulation is reached, we are able to establish the distributions of Ge content and strain within the islands. ...

Research paper thumbnail of Preserved Sm-Nd Isotopic Composition as Useful Provenance Indicators in Neoproterozoic Sandstones in the Voltaian Basin, Ghana

The provenance of sandstones derived from the Lower Voltaian Kwahu-Morago Group and the Middle Vo... more The provenance of sandstones derived from the Lower Voltaian Kwahu-Morago Group and the Middle Voltaian Oti-Pendjari Group of the Neoproterozoic Voltaian Basin are discriminated by their Sm-Nd Isotopic compositions. Plots from the Sm-Nd data suggested provenance of the Kwahu-Morago Group to be from the Birimian metasediments and associated "basin type" granitoids. The Sm-Nd studies have further revealed an average T DM model age of whole rock samples in the Kwahu-Morago Group to be 2.2 Ga which shows that this portion of the Voltaian Supergroup represents eroded remnants of "basin type" granitoids. Sm-Nd data from the Oti-Pendjari Group suggested provenance from the Birimian volcanic rocks and probably with contribution from the Pan African rocks. Its average T DM model age of whole rock samples was 2.0 Ga, which generally falls in the range of the model ages for the basement Birimian volcanic rocks as well as the model ages for the granitoid rocks and thus suggests the major source rock of the Oti-Pendjari Group as coming from the volcanic belts. The model ages for both groups seem to indicate clastic supply from an early Proterozoic crustal provenance. This study shows that whole rock isotopic analyses can also be complementary in providing an insight into the origin and development of sedimentary successions.

Research paper thumbnail of Partial wave analysis of the reaction γp→ pω and the search for nucleon resonances

An event-based partial wave analysis (PWA) of the reaction γp → pω has been performed on a high-s... more An event-based partial wave analysis (PWA) of the reaction γp → pω has been performed on a high-statistics dataset obtained using the CLAS at Jefferson Lab for center-of-mass energies from threshold up to 2.4 GeV. This analysis benefits from access to the world's first high-precision spin density matrix element measurements, available to the event-based PWA through the decay distribution of ω → π + π − π 0. The data confirm the dominance of the t-channel π 0 exchange amplitude in the forward direction. The dominant resonance contributions are consistent with the previously identified states F15(1680) and D13(1700) near threshold, as well as the G17(2190) at higher energies. Suggestive evidence for the presence of a J P = 5/2 + state around 2 GeV, a "missing" state, has also been found. Evidence for other states is inconclusive.

Research paper thumbnail of Characterizing canopy gap dynamics using hemispherical photography in emerald ash borer-impacted forests

ABSTRACT Background/Question/Methods Ash (Fraxinus species) mortality due to emerald ash borer (E... more ABSTRACT Background/Question/Methods Ash (Fraxinus species) mortality due to emerald ash borer (EAB; Agrilus planipennis) infestation may drastically alter the structure and composition of native forests. Canopy gaps resulting from dead and fallen trees can impact understory microclimates and ecological interactions. This in turn may result in altered successional trajectories, and potentially facilitate the establishment and growth of invasive plant species. From 2008 through 2010 we have taken hemispherical photographs at 5 to 20 points per plot in 129 plots within 7 different Michigan State Parks or Metroparks. Our objectives were to characterize variation among points within a plot, plots within a transect, transects within a park, among parks, and over time. We also compared canopy gap fraction with ash mortality, and understory plant growth, abundance, and composition over time. Results/Conclusions Gap fraction for a subset of plots in 2009 ranged from 0.5 to 39.3%. Photographs taken in 2010 had a median gap fraction of 6.73%; this is a slight decline from the plots in 2009, which had a median of 10.0%. Apparent decrease in median % gap fraction from 2009 to 2010 indicates possible initial stages of canopy closure as trees grow up or outward to fill in the gaps. However, initial closure may be reversed as standing dead ash trees fall, potentially creating even larger canopy gaps. Within-plot variation, analyzed for a subset of plots sampled in 2010, showed a minimum variation of 3.0% gap fraction, while the maximum was 9.4%. The median values among plots ranged from 3.7% to 9.1% gap fraction. Within-transect variation was even smaller, with a maximum variation of 5.0% A preliminary analysis of variance on a subset of the data attributed the majority of variation (75%) to differences among photographs within plots.

Research paper thumbnail of The response of birds to abrupt natural hazards as observed using weather radar

Research paper thumbnail of Absorption of the ω and ϕ Mesons in Nuclei

Physical Review Letters, 2010

Due to their long lifetimes, the ω and φ mesons are the ideal candidates for the study of possibl... more Due to their long lifetimes, the ω and φ mesons are the ideal candidates for the study of possible modifications of the in-medium meson-nucleon interaction through their absorption inside the nucleus. During the E01-112 experiment at the Thomas Jefferson National Accelerator Facility, the mesons were photoproduced from 2 H, C, Ti, Fe, and Pb targets. This paper reports the first measurement of the ratio of nuclear transparencies for the e + e − channel. The ratios indicate larger in-medium widths compared with what have been reported in other reaction channels.

Research paper thumbnail of Observation of the Helium 7 Lambda hypernucleus by the (e, e'K+) reaction

An experiment with a newly developed high-resolution kaon spectrometer (HKS) and a scattered elec... more An experiment with a newly developed high-resolution kaon spectrometer (HKS) and a scattered electron spectrometer with a novel configuration was performed in Hall C at Jefferson Lab (JLab). The ground state of a neutron-rich hypernucleus, 7 Λ He, was observed for the first time with the (e, e ′ K +) reaction with an energy resolution of ∼0.6 MeV. This resolution is the best reported to date for hypernuclear reaction spectroscopy. The 7 Λ He binding energy supplies the last missing information of the A = 7, T = 1 hypernuclear iso-triplet, providing a new input for the charge symmetry breaking (CSB) effect of ΛN potential.

Research paper thumbnail of Microstructural Evolution and Optical Properties of (InGa)(AsN) Nanostructures Synthesized by Ion Implantation

Mixed anion nitride-arsenide compound semiconductor heterostructures are promising for light-emit... more Mixed anion nitride-arsenide compound semiconductor heterostructures are promising for light-emitting devices operating throughout the near infrared range. However, due to the large N-As size difference, a limited miscibility of (InGa)(AsN) on the anion sublattice is predicted. Furthermore, alloy phase separation resulting in the formation of quantum dot-like nanostructures has been reported in GaAsN/GaAs [1] and InGaAsN/GaAs [2] superlattices. We have

Research paper thumbnail of Does a nucleon appears different when inside a nucleus?

Research paper thumbnail of Measurements of the EMC effect in few-body nuclei at large x

Recent data on the EMC effect from Jefferson Lab experiment E03103 suggest that the nuclear depen... more Recent data on the EMC effect from Jefferson Lab experiment E03103 suggest that the nuclear dependence of the high x quark distribution may depend on the local nuclear environment, rather than being a purely bulk effect. We briefly discuss the analysis and results of the E03103 experiment focusing on the light nuclei and high x region. In addition, we also discuss a future experiment at the upgraded 12 GeV Jefferson Lab facility which will further investigate the role of the local nuclear environment and the influence of detailed nuclear structure on the modification of quark distributions.

Research paper thumbnail of A flow cell for on-line monitoring of metals in natural waters by voltammetry with a mercury drop electrode

Analytica Chimica Acta, 1997

A cell was developed to enable flow analysis with voltammetric detection using a hanging mercury ... more A cell was developed to enable flow analysis with voltammetric detection using a hanging mercury drop electrode (HMDE). The flow cell has a bore of 0.7 mm in which the mercury drop is centred, and has ports for a reference and a counter electrode, and for the capillary of the ...

Research paper thumbnail of Rapid DNA library construction for functional genomic and metagenomic screening

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomi... more A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/ metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

Research paper thumbnail of The Poetry of Arnaut Daniel

Research paper thumbnail of Identification of marker chromosomes in thirteen patients using FISH probing

American Journal of Medical Genetics, 1994

Research paper thumbnail of Evaluating the activity of the RNA polymerase inhibitor myxopyronin B against Staphylococcus aureus

Myxopyronin B (MyxB) binds to the switch region of RNA polymerase (RNAP) and inhibits transcripti... more Myxopyronin B (MyxB) binds to the switch region of RNA polymerase (RNAP) and inhibits transcriptional initiation. To evaluate the potential development of MyxB as a novel class of antibiotic, we characterized the antimicrobial activity of MyxB against Staphylococcus aureus. Spontaneous MyxB resistance in S. aureus occurred at a frequency of 8 Â 10 À8 , similar to that of rifampin. The MyxBresistant mutants were found to be altered in single amino acid residues in the RNAP subunits that form the MyxB-binding site. In the presence of human serum albumin, the MyxB minimum inhibitory concentration against S. aureus increased drastically (Z128-fold) and 99.5% of MyxB was protein bound. Because of the high serum protein binding and resistance rate, we conclude that MyxB is not a viable starting point for antibiotic development.

Research paper thumbnail of Measurement of the nuclear multiplicity ratio for< span>< img height