Soheir Abd El-Salam - Academia.edu (original) (raw)
Papers by Soheir Abd El-Salam
Journal of Agronomy and Crop Science, 1993
Treatment of clay loam soil with carbaryl (sevin), thiodicarb (larvin) and methomyl (lannate) at ... more Treatment of clay loam soil with carbaryl (sevin), thiodicarb (larvin) and methomyl (lannate) at field and 10 folds the recommended doses showed a variable effect on population of total bacteria, azotobacters and nitrifying bacteria, in addition to the soil content of total soluble salts; the effect differed according to the herbicide and the dose level. The bacterial population was significantly increased in the first five days of treatment, then a decrease was recorded. The increase in the microbial population and total soluble salts were proportion to the levels of the insecticides. Moreover, supplementation of glucose to the soil caused substantial increase over that in the insecticide and/or glucose free soils.
Asian Journal of Medical and Biological Research, 2017
Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporar... more Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporary medicine and has emerged as one of the public health concerns. A total of seventy isolates of bacteria were collected from patients. The selected isolates include 33 (56.9%) from males and 25 (43.1%) from females. All bacteria were susceptible to imipenem followed by amikacin, ceftazidime, ciprofloxacin and tobramycin, respectively. The multi-drug resistant (MDR) bacterial isolates (33 isolates) divided into four groups named Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus were selected to be identified according to the keys of different identification protocols. Escherichia coli found to be the most frequent pathogen within MDR isolates followed by Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia, respectively. The MICs and MBCs of (Imipenem, Amikacin, Ceftazidime and ciprofloxacin) against the most resistant isolates were determined. Generally, P. aeruginosa (Strain No. 55) was found to be the highest resistant bacteria. Plasmid profile of Pseudomonas aeruginosa was carried out, after incubation at 37 o c for 24hr recorded no changes in plasmid and compared with incubation at 43 o c (plasmid curing) which showed complete removal of plasmid bands and the tested bacteria became more sensitive to antibiotics. This study therefore determined the prevalence, antibiotic susceptibility and plasmid patterns of P. aeruginosa strains from clinical specimens obtained from the intensive care units of Zagazig University Hospital in Egypt.
Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporar... more Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporary medicine and has emerged as one of the public health concerns. A total of seventy isolates of bacteria were collected from patients. The selected isolates include 33 (56.9%) from males and 25 (43.1%) from females.All bacteria were susceptible to imipenem followed by amikacin, ceftazidime, ciprofloxacin and tobramycin, respectively.The multi-drug resistant (MDR) bacterial isolates (33 isolates) divided into four groups named Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus were selected to be identified according to the keys of different identification protocols. Escherichia coli found to be the most frequent pathogen within MDR isolates followed by Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiellapneumonia, respectively. The MICs and MBCs of (Imipenem,Amikacin,Ceftazidime and ciprofloxacin) against the most resistant isolates were determined. Generally,P. aeruginosa (Strain No. 55) was found to be the highest resistant bacteria. Plasmid profile of Pseudomonas aeruginosa was carried out, after incubation at 37 o c for 24hr recorded no changes in plasmid and compared with incubation at 43 o c (plasmid curing) which showed complete removal of plasmid bands and the tested bacteria became more sensitive to antibiotics. This study therefore determined the prevalence, antibiotic susceptibility and plasmid patterns of P.aeruginosa strains from clinical specimens obtained from the intensive care units of Zagazig University Hospital in Egypt.
World Journal of Pharmaceutical and life sciences, Apr 8, 2005
Background: Hepatitis C virus (HCV) is considered the most common etiology of chronic liver disea... more Background: Hepatitis C virus (HCV) is considered the most common etiology of chronic liver disease in Egypt, which may progress to cirrhosis and hepatocellular carcinoma (HCC). Previous studies have documented an association between Helicobacter pylori (H. pylori) infection and HCV. Objective: This study aimed to investigate the role of Helicobacter pylori (H. pylori) seropositivity in the treatment of patients with hepatitis C virus (HCV) infection and the effect of this co-infection on response to interferon-α and ribavirin therapy. Methods: The presence of H. pylori was tested using a commercially available enzyme immunoassay in serum samples from 49 patients with chronic hepatitis C. Clinical features, HCV markers and response of HCV to interferon-α and ribavirin therapy were compared between H. pylori seropositivity and H. pylori seronegativity patients. Results: H.pylori antigen was detected in 24(49.0%) of 50 HCV patients. There was no association between H. pylori seropositivity in the liver and age, gender of patients, liver function tests, AFP levels or viral load. The sustained response rate for HCV clearance following interferon-α and ribavirin treatment did not differ between patients with and without anti-H. pylori seropositivity. Conclusion: No correlation between coexistent H.Pylori infection and clinical course of Hepatitis C Virus and suggest an association between this bacterium and progression of liver fibrosis.
Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2021
Contamination and poisoning of food products are major causes of morbidity and mortality in devel... more Contamination and poisoning of food products are major causes of morbidity and mortality in developing countries (Sapkota et al., 2012). Contamination of food products by Gram negative bacteria especially, Salmonella spp., Escherichia coli and Pseudomonas aeruginosa as well as Gram positives especially, Bacillus cereus, clostridium species, Staphylococcus aureus and Listeria monocytogenes is one of the main causes of food poisoning (Silva & Lidon, 2017 and Mostafa et al., 2018).
A big problem in intensive care units (ICU) is caused by antibiotic resistant bacterial nosocomia... more A big problem in intensive care units (ICU) is caused by antibiotic resistant bacterial nosocomial infections. Acinetobacter are among the most challenging bacterial pathogens and to help in formulating antibiotic policy for better management of patients with bacterial diseases. The data showed that 93.3% of bacterial isolates were resistant to ampicillin while 80% and 66.7% of bacterial isolates were resistant to ceftazidime and sulphamethoxazole/trimethoprim, respectively. Identification of multi-resistant isolates according to morphological and biochemical characteristics, Acinetobacter baumannii found to be the most frequent pathogen representing 66.7% followed by Acinetobacter calcoaceticus and Acinetobacter lwoffii with 20% and 13.3% percentages, respectively. The main objective of this study was studying the susceptibility of multi-drug resistant isolates to different ten essential oils derived from different parts of ten medicinal plant species traditionally used in Egyptian...
Egyptian Journal of Microbiology, 2018
The Egyptian Journal of Hospital Medicine, 2018
Background: The green synthesis of cadmium sulfide (CdS) nanoparticles has been regarded as the m... more Background: The green synthesis of cadmium sulfide (CdS) nanoparticles has been regarded as the most promising technique for their prospective applications in biological system. Aim of the work: In this study isolation of different bacterial strains from stool samples of healthy volunteer, selection of the most efficient bacterial strains able to reduce cadmium sulfide metal into nanoparticles. Characterization of biosynthesized metal nanoparticles by standard analytical methods. Mediating the biosynthesized cadmium sulfide nanoparticles in medical applications in comparison to those produced by chemical methods. Materials and methods: Extracellular Escherichia coli E-30 and Klebsiella pneumoniae K-6 isolated from stool samples were the strains used for biosynthesis. Cadmium sulfide nanoparticles were also produced by wet chemical method. The characterizations of cadmium sulfide nanoparticles were done by using UV-Visible Spectroscopy, Transmission electron microscopy (TEM), energy dispersive x-ray (EDX) and Fourier transform infrared spectroscopy (FT-IR). Results: Escherichia coli E-30 has shown to be efficient in synthesizing cadmium sulfide nanoparticles where CdS nanoparticles were with average size ranging from 3.2 to 44.9 nm while average size of CdS nanoparticle was synthesized by Klebsiella pneumoniae K-6 ranging from 8.5 to 44.9 nm. While cadmium sulfide nanoparticles synthesized by wet chemical method, ranging from 8.77 to 16.50 nm. Biosynthesized cadmium sulfide nanoparticles by Escherichia coli E-30 showed highest antimicrobial activity on Aspergillus fumigatus, Geotricum candidum, Bacillus subtilis, Staphylococcus aureus and Escherichia coli than chemical synthesized of CdS nanoparticles. Conclusion: Escherichia coli and Klebsiella pneumoniae isolated from stool samples had the ability to produce cadmium sulfide nanoparticles. This kind of microorganisms can be used for synthesis of nanoparticles and heavy metal absorption for detoxification of environment.
Egyptian Journal of Botany, 2017
The main water source is the River Nile in Benha city.The Department of Environment (D.O.E.) moni... more The main water source is the River Nile in Benha city.The Department of Environment (D.O.E.) monitors the river basins for major contaminant sources (Chan, 2004), while the quality of the raw water reservoir is monitored by state water monitoring and controlling authorities (Hasbiyana, 2008). The main source of filtered water for Benha city is New Benha filtered water treatment plant of Atripe area with design capacity (68000 m 3 / day) which produces (56,000 m 3 / day) to pump water into the water networks. Man consumes water to survive. Clean drinking water is always needed for human health. The mortality of people aworld due to water associated diseases exceeds 5 million people per year (UN-Water, 2013). Therefore, water quality control is very important in many parts of the world (WHO, 2011). Determination of physical and chemical (inorganic and organic) parameters is very important to evaluate water quality (Dissmeyer, 2000). Contaminants in the water W ATER quality assessment of drinking water has been studied in Benha city, Qalubia Governorate, Egypt between April 2015 to March 2016 to evaluate the quality of different types of water according to physicochemical, chemical and bacteriological aspects. Twenty four samples were collected during the four seasons from different places in Benha City. Two kinds were analyzed; Benha filtered water and raw water of River Nile. The results indicated that the average values of physicochemical, heavy metals, trihalomethanes and bacteriological parameters in Benha filtered water treatment were within Egyptian standard (2007) and the World Health Organization (WHO) of water quality with comparison to raw water. The dominant bacterial isolates in the raw water samples were Escherichia coli and Enterobacter aerogenes; while the filtered water samples were 100% free from pathogenic bacteria and coliforms.
International Journal of Advanced Research, 2019
Background and Aim: Kombucha Green Tea (KGT) a health promoting fermented beverage traditionally ... more Background and Aim: Kombucha Green Tea (KGT) a health promoting fermented beverage traditionally made by fermenting a sweetened green tea with a symbiotic culture of yeast species and acetic acid bacteria. This drink is composed of some probiotics such as acetic acid bacteria and lactic acid bacteria and also tea polyphenols, sugars, ethanol, water soluble vitamins, a variety of micronutrients produced during fermentation and organic acids as acetic and gluconic acids which are the main metabolites produced. The broth result from fermentation has beneficial effects such as regulation of gastrointestinal and glandular activities, positive influence on the cholesterol level, toxin excretion and blood cleansing, diabetes, aging problems, arteriosclerosis and it has been thought to be a prophylactic and therapeutic beneficial agent to human health from weight loss to curing cancer. And so, this study aims to use kombucha green tea as a natural remedy as result of its antioxidant and antimicrobial activities. Methodsusing:Radical scavenging assays by 2, 2-diphenyl-1 picrylhydrazyl (DPPH), and scavenging hydrogen peroxide assay were used to determine the antioxidant activity of the KGT spectrophotometrically.Evaluation of antimicrobial activity of KGT against different pathogenic bacteria that cause human diseases using disk diffusion method. Results:Data showed increasing of the antioxidant activity of the fermented KGT gradually within fermentation time reach to the maximum increase observed on the 14 th day with slightly decrease at its end.KGT exhibited higher hydrogen peroxide scavenging activity at 14 th day of fermentation by 38.8%.increasing of KGT concentration lead to increasing % of inhibition of DPPH within fermentation time that reach to maximum at 14 th day obtaining IC 50 0.1196± 0.0290 (P<0.05). KGT obtained clearly antibacterial activity at 14 th day of fermentation on klebsiella pneuonimae (K55) and on Escherichi coli (C95). Conclusion:this study revealed that the kombucha green tea(KGT) possess great with antioxidant and antimicrobial activities.These findings provide additional support for the traditional use of Kombucha tea in the treatment of metabolic diseases and different types of cancer.
Egyptian Journal of Microbiology, 2017
B OTH hepatitis C virus and Helicobacter pylori infections are commonly found in Egypt. Correlati... more B OTH hepatitis C virus and Helicobacter pylori infections are commonly found in Egypt. Correlation among Helicobacter pylori and HCV has been assisted. This work aim was to research H. pylori DNA inside the liver tissue of Egyptian suffers with persistent hepatitis C and find the relation among HP invasion and HCV. This prospective study was conducted with 49 participants. Helicobacter pylori Standard Kit (H.pylori) genome is designed for the in vitro H.pylori genomes from liver tissues patients with chronic hepatitis C quantitatively. A TaqMan® principle was used by the primer and probe. During PCR amplification, primers of bidirectional hybridize to the H.pylori DNA. DNA probe was Labeled with a 5`-dye and a 3`-quencher forms the fluorogenic probe. During PCR amplification, the indicator dye and the quencher are separated and the probe is split. The increase in fluorescence can be detected by PCR platforms. The bacterial DNA was existed in the liver specimens approximately 44.9% of patients. The DNA of bacteria in hepatic tissue was highly recurrent in patients with progressed fibrosis (54.5% vs. 45.5 %, P = 0.02). Meanwhile, the infective dose of HCV was higher in sufferers with HP DNA in liver tissue compared to patients with no HP DNA in liver tissue (9.0×105 vs. 0.05×105). There hasn't been a relation among the tested bacteria and some factors as age, sex and (LFT) liver function tests while (AFP) α-fetoprotein levels did not differ between patients in absence or presence H.pylori DNA. Conclusion: There was coexistence of HP infection, HCV infective dose and liver fibrosis.
Egyptian Journal of Microbiology, 2016
ACTERIA are considered the dominant microorganisms, which cause urinary tract infection (UTI) of ... more ACTERIA are considered the dominant microorganisms, which cause urinary tract infection (UTI) of both male and female. The development of multi-drug resistance phenomenon in bacteria leads to the necessity of finding an alternative remedy. Probiotics are suggested as a suitable and appropriate solution. In this study, cell free supernatant CFS of Enterococcus faeciumNM 2 is examined for its inhibitory effect against clinical urogenital bacterial strains and Candida albicans isolated from urinary tract infection infections. CFS obtained from these probiotic bacteriashowed a promising antibacterial and anticandidial results. E. faeciumNM 2 possessed capability to be used as a probiotic against medical bacteria isolated from urogenital infections.
Background: The increased use of antibiotics in recent times has resulted in the development of r... more Background: The increased use of antibiotics in recent times has resulted in the development of resistance to antibiotics. The significant clinical implication of resistance has led to heightened interest in the study of bacterial resistance. Purpose: This study was carried out to evaluate the effects of antibiotic-combinations against some community isolates of multiple antibiotic-resistant Staphylococcus aureus and also to determine if the observed resistance is plasmid or chromosomally-mediated. Methods: Swabs were collected from nostrils of one hundred healthy human subjects, streaked in mannitol-salt agar, and incubated. The isolates were characterized to species level and fifty three (53) of these isolates were identified as S. aureus. Antibiotic sensitivity of the isolates was determined using agar diffusion method. The combined in-vitro antimicrobial activities of gentamicin and other antibiotics (flucloxaccillin, cephalexin, and clindamycin) against the resistant strains (i...
Environmental Science and Pollution Research, 2019
The present study describes the impregnation of coffee extract (CE) into bacterial cellulose synt... more The present study describes the impregnation of coffee extract (CE) into bacterial cellulose synthesized from kombucha tea fungus (KBC) of different cellulose content, incubated for different incubation periods (2, 4, and 10 days), to prepare biocomposites having the potential for wound healing applications. Total polyphenols in hydroalcoholic extracts from ground roasted coffee and its release from the prepared biocomposites were determined as gallic acid equivalent. The polyphenols content was found to be 13.66 mg/g and the minimum inhibitory concentration (MIC) of the CE was determined using colony-forming unit (CFU) method against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus where the growth inhibition was 86 and 97% respectively. Biocomposites (KBC/CE) with the lowest cellulose and CE content showed the highest wet tensile stress (3.35 MPa), absorption of pseudo extracellular fluid (154.32% ± 4.84), and water vapor transmission rate (3184.94 ± 198.07 g/m2/day), whereas it showed the lowest polyphenols’ release (51.85% ± 2.94)when immersed in PBS buffer of pH 7.4. The impregnation of CE into KBC provided biocomposites that can enlarge the range of BC in the biomedical application.
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 2017
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology), 2017
Egyptian Journal of Microbiology, 2016
Journal of Agronomy and Crop Science, 1993
Treatment of clay loam soil with carbaryl (sevin), thiodicarb (larvin) and methomyl (lannate) at ... more Treatment of clay loam soil with carbaryl (sevin), thiodicarb (larvin) and methomyl (lannate) at field and 10 folds the recommended doses showed a variable effect on population of total bacteria, azotobacters and nitrifying bacteria, in addition to the soil content of total soluble salts; the effect differed according to the herbicide and the dose level. The bacterial population was significantly increased in the first five days of treatment, then a decrease was recorded. The increase in the microbial population and total soluble salts were proportion to the levels of the insecticides. Moreover, supplementation of glucose to the soil caused substantial increase over that in the insecticide and/or glucose free soils.
Asian Journal of Medical and Biological Research, 2017
Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporar... more Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporary medicine and has emerged as one of the public health concerns. A total of seventy isolates of bacteria were collected from patients. The selected isolates include 33 (56.9%) from males and 25 (43.1%) from females. All bacteria were susceptible to imipenem followed by amikacin, ceftazidime, ciprofloxacin and tobramycin, respectively. The multi-drug resistant (MDR) bacterial isolates (33 isolates) divided into four groups named Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus were selected to be identified according to the keys of different identification protocols. Escherichia coli found to be the most frequent pathogen within MDR isolates followed by Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia, respectively. The MICs and MBCs of (Imipenem, Amikacin, Ceftazidime and ciprofloxacin) against the most resistant isolates were determined. Generally, P. aeruginosa (Strain No. 55) was found to be the highest resistant bacteria. Plasmid profile of Pseudomonas aeruginosa was carried out, after incubation at 37 o c for 24hr recorded no changes in plasmid and compared with incubation at 43 o c (plasmid curing) which showed complete removal of plasmid bands and the tested bacteria became more sensitive to antibiotics. This study therefore determined the prevalence, antibiotic susceptibility and plasmid patterns of P. aeruginosa strains from clinical specimens obtained from the intensive care units of Zagazig University Hospital in Egypt.
Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporar... more Nosocomial infections and antibiotic resistance are serious and growing phenomenon in contemporary medicine and has emerged as one of the public health concerns. A total of seventy isolates of bacteria were collected from patients. The selected isolates include 33 (56.9%) from males and 25 (43.1%) from females.All bacteria were susceptible to imipenem followed by amikacin, ceftazidime, ciprofloxacin and tobramycin, respectively.The multi-drug resistant (MDR) bacterial isolates (33 isolates) divided into four groups named Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus were selected to be identified according to the keys of different identification protocols. Escherichia coli found to be the most frequent pathogen within MDR isolates followed by Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiellapneumonia, respectively. The MICs and MBCs of (Imipenem,Amikacin,Ceftazidime and ciprofloxacin) against the most resistant isolates were determined. Generally,P. aeruginosa (Strain No. 55) was found to be the highest resistant bacteria. Plasmid profile of Pseudomonas aeruginosa was carried out, after incubation at 37 o c for 24hr recorded no changes in plasmid and compared with incubation at 43 o c (plasmid curing) which showed complete removal of plasmid bands and the tested bacteria became more sensitive to antibiotics. This study therefore determined the prevalence, antibiotic susceptibility and plasmid patterns of P.aeruginosa strains from clinical specimens obtained from the intensive care units of Zagazig University Hospital in Egypt.
World Journal of Pharmaceutical and life sciences, Apr 8, 2005
Background: Hepatitis C virus (HCV) is considered the most common etiology of chronic liver disea... more Background: Hepatitis C virus (HCV) is considered the most common etiology of chronic liver disease in Egypt, which may progress to cirrhosis and hepatocellular carcinoma (HCC). Previous studies have documented an association between Helicobacter pylori (H. pylori) infection and HCV. Objective: This study aimed to investigate the role of Helicobacter pylori (H. pylori) seropositivity in the treatment of patients with hepatitis C virus (HCV) infection and the effect of this co-infection on response to interferon-α and ribavirin therapy. Methods: The presence of H. pylori was tested using a commercially available enzyme immunoassay in serum samples from 49 patients with chronic hepatitis C. Clinical features, HCV markers and response of HCV to interferon-α and ribavirin therapy were compared between H. pylori seropositivity and H. pylori seronegativity patients. Results: H.pylori antigen was detected in 24(49.0%) of 50 HCV patients. There was no association between H. pylori seropositivity in the liver and age, gender of patients, liver function tests, AFP levels or viral load. The sustained response rate for HCV clearance following interferon-α and ribavirin treatment did not differ between patients with and without anti-H. pylori seropositivity. Conclusion: No correlation between coexistent H.Pylori infection and clinical course of Hepatitis C Virus and suggest an association between this bacterium and progression of liver fibrosis.
Egyptian Academic Journal of Biological Sciences, G. Microbiology, 2021
Contamination and poisoning of food products are major causes of morbidity and mortality in devel... more Contamination and poisoning of food products are major causes of morbidity and mortality in developing countries (Sapkota et al., 2012). Contamination of food products by Gram negative bacteria especially, Salmonella spp., Escherichia coli and Pseudomonas aeruginosa as well as Gram positives especially, Bacillus cereus, clostridium species, Staphylococcus aureus and Listeria monocytogenes is one of the main causes of food poisoning (Silva & Lidon, 2017 and Mostafa et al., 2018).
A big problem in intensive care units (ICU) is caused by antibiotic resistant bacterial nosocomia... more A big problem in intensive care units (ICU) is caused by antibiotic resistant bacterial nosocomial infections. Acinetobacter are among the most challenging bacterial pathogens and to help in formulating antibiotic policy for better management of patients with bacterial diseases. The data showed that 93.3% of bacterial isolates were resistant to ampicillin while 80% and 66.7% of bacterial isolates were resistant to ceftazidime and sulphamethoxazole/trimethoprim, respectively. Identification of multi-resistant isolates according to morphological and biochemical characteristics, Acinetobacter baumannii found to be the most frequent pathogen representing 66.7% followed by Acinetobacter calcoaceticus and Acinetobacter lwoffii with 20% and 13.3% percentages, respectively. The main objective of this study was studying the susceptibility of multi-drug resistant isolates to different ten essential oils derived from different parts of ten medicinal plant species traditionally used in Egyptian...
Egyptian Journal of Microbiology, 2018
The Egyptian Journal of Hospital Medicine, 2018
Background: The green synthesis of cadmium sulfide (CdS) nanoparticles has been regarded as the m... more Background: The green synthesis of cadmium sulfide (CdS) nanoparticles has been regarded as the most promising technique for their prospective applications in biological system. Aim of the work: In this study isolation of different bacterial strains from stool samples of healthy volunteer, selection of the most efficient bacterial strains able to reduce cadmium sulfide metal into nanoparticles. Characterization of biosynthesized metal nanoparticles by standard analytical methods. Mediating the biosynthesized cadmium sulfide nanoparticles in medical applications in comparison to those produced by chemical methods. Materials and methods: Extracellular Escherichia coli E-30 and Klebsiella pneumoniae K-6 isolated from stool samples were the strains used for biosynthesis. Cadmium sulfide nanoparticles were also produced by wet chemical method. The characterizations of cadmium sulfide nanoparticles were done by using UV-Visible Spectroscopy, Transmission electron microscopy (TEM), energy dispersive x-ray (EDX) and Fourier transform infrared spectroscopy (FT-IR). Results: Escherichia coli E-30 has shown to be efficient in synthesizing cadmium sulfide nanoparticles where CdS nanoparticles were with average size ranging from 3.2 to 44.9 nm while average size of CdS nanoparticle was synthesized by Klebsiella pneumoniae K-6 ranging from 8.5 to 44.9 nm. While cadmium sulfide nanoparticles synthesized by wet chemical method, ranging from 8.77 to 16.50 nm. Biosynthesized cadmium sulfide nanoparticles by Escherichia coli E-30 showed highest antimicrobial activity on Aspergillus fumigatus, Geotricum candidum, Bacillus subtilis, Staphylococcus aureus and Escherichia coli than chemical synthesized of CdS nanoparticles. Conclusion: Escherichia coli and Klebsiella pneumoniae isolated from stool samples had the ability to produce cadmium sulfide nanoparticles. This kind of microorganisms can be used for synthesis of nanoparticles and heavy metal absorption for detoxification of environment.
Egyptian Journal of Botany, 2017
The main water source is the River Nile in Benha city.The Department of Environment (D.O.E.) moni... more The main water source is the River Nile in Benha city.The Department of Environment (D.O.E.) monitors the river basins for major contaminant sources (Chan, 2004), while the quality of the raw water reservoir is monitored by state water monitoring and controlling authorities (Hasbiyana, 2008). The main source of filtered water for Benha city is New Benha filtered water treatment plant of Atripe area with design capacity (68000 m 3 / day) which produces (56,000 m 3 / day) to pump water into the water networks. Man consumes water to survive. Clean drinking water is always needed for human health. The mortality of people aworld due to water associated diseases exceeds 5 million people per year (UN-Water, 2013). Therefore, water quality control is very important in many parts of the world (WHO, 2011). Determination of physical and chemical (inorganic and organic) parameters is very important to evaluate water quality (Dissmeyer, 2000). Contaminants in the water W ATER quality assessment of drinking water has been studied in Benha city, Qalubia Governorate, Egypt between April 2015 to March 2016 to evaluate the quality of different types of water according to physicochemical, chemical and bacteriological aspects. Twenty four samples were collected during the four seasons from different places in Benha City. Two kinds were analyzed; Benha filtered water and raw water of River Nile. The results indicated that the average values of physicochemical, heavy metals, trihalomethanes and bacteriological parameters in Benha filtered water treatment were within Egyptian standard (2007) and the World Health Organization (WHO) of water quality with comparison to raw water. The dominant bacterial isolates in the raw water samples were Escherichia coli and Enterobacter aerogenes; while the filtered water samples were 100% free from pathogenic bacteria and coliforms.
International Journal of Advanced Research, 2019
Background and Aim: Kombucha Green Tea (KGT) a health promoting fermented beverage traditionally ... more Background and Aim: Kombucha Green Tea (KGT) a health promoting fermented beverage traditionally made by fermenting a sweetened green tea with a symbiotic culture of yeast species and acetic acid bacteria. This drink is composed of some probiotics such as acetic acid bacteria and lactic acid bacteria and also tea polyphenols, sugars, ethanol, water soluble vitamins, a variety of micronutrients produced during fermentation and organic acids as acetic and gluconic acids which are the main metabolites produced. The broth result from fermentation has beneficial effects such as regulation of gastrointestinal and glandular activities, positive influence on the cholesterol level, toxin excretion and blood cleansing, diabetes, aging problems, arteriosclerosis and it has been thought to be a prophylactic and therapeutic beneficial agent to human health from weight loss to curing cancer. And so, this study aims to use kombucha green tea as a natural remedy as result of its antioxidant and antimicrobial activities. Methodsusing:Radical scavenging assays by 2, 2-diphenyl-1 picrylhydrazyl (DPPH), and scavenging hydrogen peroxide assay were used to determine the antioxidant activity of the KGT spectrophotometrically.Evaluation of antimicrobial activity of KGT against different pathogenic bacteria that cause human diseases using disk diffusion method. Results:Data showed increasing of the antioxidant activity of the fermented KGT gradually within fermentation time reach to the maximum increase observed on the 14 th day with slightly decrease at its end.KGT exhibited higher hydrogen peroxide scavenging activity at 14 th day of fermentation by 38.8%.increasing of KGT concentration lead to increasing % of inhibition of DPPH within fermentation time that reach to maximum at 14 th day obtaining IC 50 0.1196± 0.0290 (P<0.05). KGT obtained clearly antibacterial activity at 14 th day of fermentation on klebsiella pneuonimae (K55) and on Escherichi coli (C95). Conclusion:this study revealed that the kombucha green tea(KGT) possess great with antioxidant and antimicrobial activities.These findings provide additional support for the traditional use of Kombucha tea in the treatment of metabolic diseases and different types of cancer.
Egyptian Journal of Microbiology, 2017
B OTH hepatitis C virus and Helicobacter pylori infections are commonly found in Egypt. Correlati... more B OTH hepatitis C virus and Helicobacter pylori infections are commonly found in Egypt. Correlation among Helicobacter pylori and HCV has been assisted. This work aim was to research H. pylori DNA inside the liver tissue of Egyptian suffers with persistent hepatitis C and find the relation among HP invasion and HCV. This prospective study was conducted with 49 participants. Helicobacter pylori Standard Kit (H.pylori) genome is designed for the in vitro H.pylori genomes from liver tissues patients with chronic hepatitis C quantitatively. A TaqMan® principle was used by the primer and probe. During PCR amplification, primers of bidirectional hybridize to the H.pylori DNA. DNA probe was Labeled with a 5`-dye and a 3`-quencher forms the fluorogenic probe. During PCR amplification, the indicator dye and the quencher are separated and the probe is split. The increase in fluorescence can be detected by PCR platforms. The bacterial DNA was existed in the liver specimens approximately 44.9% of patients. The DNA of bacteria in hepatic tissue was highly recurrent in patients with progressed fibrosis (54.5% vs. 45.5 %, P = 0.02). Meanwhile, the infective dose of HCV was higher in sufferers with HP DNA in liver tissue compared to patients with no HP DNA in liver tissue (9.0×105 vs. 0.05×105). There hasn't been a relation among the tested bacteria and some factors as age, sex and (LFT) liver function tests while (AFP) α-fetoprotein levels did not differ between patients in absence or presence H.pylori DNA. Conclusion: There was coexistence of HP infection, HCV infective dose and liver fibrosis.
Egyptian Journal of Microbiology, 2016
ACTERIA are considered the dominant microorganisms, which cause urinary tract infection (UTI) of ... more ACTERIA are considered the dominant microorganisms, which cause urinary tract infection (UTI) of both male and female. The development of multi-drug resistance phenomenon in bacteria leads to the necessity of finding an alternative remedy. Probiotics are suggested as a suitable and appropriate solution. In this study, cell free supernatant CFS of Enterococcus faeciumNM 2 is examined for its inhibitory effect against clinical urogenital bacterial strains and Candida albicans isolated from urinary tract infection infections. CFS obtained from these probiotic bacteriashowed a promising antibacterial and anticandidial results. E. faeciumNM 2 possessed capability to be used as a probiotic against medical bacteria isolated from urogenital infections.
Background: The increased use of antibiotics in recent times has resulted in the development of r... more Background: The increased use of antibiotics in recent times has resulted in the development of resistance to antibiotics. The significant clinical implication of resistance has led to heightened interest in the study of bacterial resistance. Purpose: This study was carried out to evaluate the effects of antibiotic-combinations against some community isolates of multiple antibiotic-resistant Staphylococcus aureus and also to determine if the observed resistance is plasmid or chromosomally-mediated. Methods: Swabs were collected from nostrils of one hundred healthy human subjects, streaked in mannitol-salt agar, and incubated. The isolates were characterized to species level and fifty three (53) of these isolates were identified as S. aureus. Antibiotic sensitivity of the isolates was determined using agar diffusion method. The combined in-vitro antimicrobial activities of gentamicin and other antibiotics (flucloxaccillin, cephalexin, and clindamycin) against the resistant strains (i...
Environmental Science and Pollution Research, 2019
The present study describes the impregnation of coffee extract (CE) into bacterial cellulose synt... more The present study describes the impregnation of coffee extract (CE) into bacterial cellulose synthesized from kombucha tea fungus (KBC) of different cellulose content, incubated for different incubation periods (2, 4, and 10 days), to prepare biocomposites having the potential for wound healing applications. Total polyphenols in hydroalcoholic extracts from ground roasted coffee and its release from the prepared biocomposites were determined as gallic acid equivalent. The polyphenols content was found to be 13.66 mg/g and the minimum inhibitory concentration (MIC) of the CE was determined using colony-forming unit (CFU) method against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus where the growth inhibition was 86 and 97% respectively. Biocomposites (KBC/CE) with the lowest cellulose and CE content showed the highest wet tensile stress (3.35 MPa), absorption of pseudo extracellular fluid (154.32% ± 4.84), and water vapor transmission rate (3184.94 ± 198.07 g/m2/day), whereas it showed the lowest polyphenols’ release (51.85% ± 2.94)when immersed in PBS buffer of pH 7.4. The impregnation of CE into KBC provided biocomposites that can enlarge the range of BC in the biomedical application.
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 2017
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology), 2017
Egyptian Journal of Microbiology, 2016