Abhijit Mondal - Academia.edu (original) (raw)

Papers by Abhijit Mondal

Research paper thumbnail of ge의 지배구조와 국제조세전략

Korean Accounting Association, Aug 1, 2020

Research paper thumbnail of Cyanobacteria: A Biocatalyst in Microbial Fuel Cell for Sustainable Electricity Generation

Waste to Sustainable Energy, 2019

Research paper thumbnail of Effects of graphene on thermal properties and thermal stability of polycarbonate/graphene nanocomposite

Journal of Thermoplastic Composite Materials, 2021

Polycarbonate (PC) /graphene nanocomposite was prepared using multilayer graphene (MLG) with load... more Polycarbonate (PC) /graphene nanocomposite was prepared using multilayer graphene (MLG) with loadings of 0.5, 1, and 3 wt% via melt mixing process. Morphological, structural, and thermal properties of the PC/MLG nanocomposites are investigated to look into the influence of MLG on the nanocomposite. A significant increase (∼6.4°C) in glass transition temperature is observed upon the addition of 3 wt% of MLG into the polycarbonate matrix. This increase in glass transition temperature may be due to the interaction between the MLG and polycarbonate polymer matrix. The specific heat capacity of pure PC and PC/MLG nanocomposites varies linearly with temperature below their glass transition. Upon the addition of MLGs, the overall thermal stability of PC/MLG nanocomposites increases with MLG loadings. A maximum increase about 29.23°C in T onset of thermal decomposition is observed in PC/MLG nanocomposite having 3 wt% of MLG loading. The activation energy ( Ea) of thermal decomposition is al...

Research paper thumbnail of Federated Adaptive Bitrate Live Streaming over Locality Sensitive Playback Coalitions

Live video broadcasts to particular communities or targeted audiences many-at-a-times indulge a c... more Live video broadcasts to particular communities or targeted audiences many-at-a-times indulge a cluster of localities from where the end-users are interested in participating. In this paper, we leverage this idea to develop a system called Federated Live Streaming over DASH (FLiDASH) which forms end-users coalitions based on the locality of the network connectivity (like a set of end-users behind a common core-network service gateway). In FLiDASH, the coalition members stream the live data collectively based on a federated adaptive bitrate streaming mechanism where the coalition as a whole decides the optimal bitrate for video streaming and distributes the download overhead among its members. We have thoroughly evaluated FLiDASH in an emulated setup, and observe a 40% improvement in the live streaming QoE with a 20% reduction in the network traffic usage.

Research paper thumbnail of Design of Grid Connected H Bridge Multi Level Inverter with PV System

This paper describes the various topologies and controlling strategies used in multi level invert... more This paper describes the various topologies and controlling strategies used in multi level inverter. MLI are used to improve the waveform output of the inverter to be nearly to sinusoidal so that the harmonic injection by that inverter is minimum. In this paper implement a multi level inverter used in photo voltaic grid integration. A cascaded asymmetric multi level inverter is proposed which contains minimum no. of switches and it can be employed in AC application using solar energy. The proposed topology consists of 25 output level using 10 switches with nearly sinusoidal output, thereby reducing gate driver circuit and layout of optimizing circuit. All the simulation are carried out by using MATLAB 2010a.

Research paper thumbnail of Toward cardiac tissue characterization using machine learning and light-scattering spectroscopy

Journal of Biomedical Optics, 2021

Research paper thumbnail of An ns3-based Energy Module of 5G NR User Equipments for Millimeter Wave Networks

IEEE INFOCOM 2021 - IEEE Conference on Computer Communications Workshops (INFOCOM WKSHPS), 2021

This poster presents the design, development and test results of an energy consumption analysis m... more This poster presents the design, development and test results of an energy consumption analysis module developed over ns3 Millimeter Wave (mmWave) communication for analyzing power consumption for 5G New Radio (NR) User Equipment (UE) during both continuous and discontinuous packet receptions. This module is important to analyze and explore the energy consumption behavior of the 5G communication protocols under the NR technology. The developed module includes the complete Radio Resource Control (RRC) state machine for 5G NR recommended by 3GPP Specification 38.840. To the best of our knowledge, the designed module is the first of its kind that provides a comprehensive energy analysis for the 5G NR UEs over mmWave communication.

Research paper thumbnail of System call interception for serverless isolation

Proceedings of the SIGCOMM '20 Poster and Demo Sessions, 2020

Research paper thumbnail of Multi-Label Classification of Parrott’s Emotions

Proceedings of the 33rd International Conference on Software Engineering and Knowledge Engineering, 2021

Mining for latent emotions embedded in tweets can offer clues about users' affective state on a b... more Mining for latent emotions embedded in tweets can offer clues about users' affective state on a broad range of topics ranging from their mental health to political opinions. This paper presents a multi-class supervised learning approach to group tweets into six emotions (joy, sadness, anger, fear, love, and surprise) defined according to the Parrott's framework. After extensive pre-processing, linguistic and metadata features extracted from a corpus of tweets are used to train popular machine learning classifiers. The performance of these classifiers is evaluated using accuracy, sensitivity, and specificity computed based on a multi-class confusion matrix approach. Our framework can detect common emotions of joy and sadness with excellent accuracy (> 90%), anger and fear with moderate accuracy (75% − 85%), and love and surprise with lower accuracy (50% − 60%). Overall, the accuracy of our framework still outperforms that of contemporary approaches for all the six emotions. Further analysis of an example multi-class confusion matrix indicates that lower accuracy values for love and surprise may arise because love is often confused with joy, whereas surprise is mixed up with the positive emotion of joy and the negative emotion of fear. Moreover, this confusion could be attributed to an under-representation of these emotions in the data. This highlights the need for building high-quality, balanced benchmark data sets for training multi-label emotion classifiers.

Research paper thumbnail of Effect of Pleurotus ostreatus (Jacq.) and Trichoderma harzianum (Rifai) on Meloidogyne incognita (Kofoid White) in tomato (Solanum lycopersicum Mill.)

Acta Scientiarum. Biological Sciences, 2020

Two isolations of fungi from a bank of microorganisms in the Biological Sciences Laboratory at Es... more Two isolations of fungi from a bank of microorganisms in the Biological Sciences Laboratory at Escuela Superior Politécnica de Chimborazo were tested on the galling caused by Meloidogyne incognita in tomato seedlings grown in pots with substrate infested with a suspension of nematodes, with approximately 2000 juvenile stages (J2) from root galls of plants infested with M. incognita, taken from the Nematology laboratory of the Ecuadorian Agricultural Quality Assurance Agency (AGROCALIDAD). Pleurotus ostreatus was a fungus with nematicidal characteristics through production of toxins; while Trichoderma harzianum is a widely known fungus, although it is a plant growth promoter rather than a nematicide. The two fungi were formulated in wheat straw and rabbit manure. A complete randomized design (CRD) with three replications was used, with a chemical control (Fenamiphos) and an absolute control. Five grams of each formulation was applied per plant before the transplant. The number of g...

Research paper thumbnail of Localization of the sinoatrial and atrioventricular nodal region in neonatal and juvenile ovine hearts

PLOS ONE, 2020

Localization of the components of the cardiac conduction system (CCS) is essential for many thera... more Localization of the components of the cardiac conduction system (CCS) is essential for many therapeutic procedures in cardiac surgery and interventional cardiology. While histological studies provided fundamental insights into CCS localization, this information is incomplete and difficult to translate to aid in intraprocedural localization. To advance our understanding of CCS localization, we set out to establish a framework for quantifying nodal region morphology. Using this framework, we quantitatively analyzed the sinoatrial node (SAN) and atrioventricular node (AVN) in ovine with postmenstrual age ranging from 4.4 to 58.3 months. In particular, we studied the SAN and AVN in relation to the epicardial and endocardial surfaces, respectively. Using anatomical landmarks, we excised the nodes and adjacent tissues, sectioned those at a thickness of 4 μm at 100 μm intervals, and applied Masson's trichrome stain to the sections. These sections were then imaged, segmented to identify nodal tissue, and analyzed to quantify nodal depth and superficial tissue composition. The minimal SAN depth ranged between 20 and 926 μm. AVN minimal depth ranged between 59 and 1192 μm in the AVN extension region, 49 and 980 μm for the compact node, and 148 and 888 μm for the transition to His Bundle region. Using a logarithmic regression model, we found that minimal depth increased logarithmically with age for the AVN (R 2 = 0.818, P = 0.002). Also, the myocardial overlay of the AVN was heterogeneous within different regions and decreased with increasing age. Age associated alterations of SAN minimal depth were insignificant. Our study presents examples of characteristic tissue patterns superficial to the AVN and within the SAN. We suggest that the presented framework provides quantitative information for CCS localization. Our studies indicate that procedural methods and localization approaches in regions near the AVN should account for the age of patients in cardiac surgery and interventional cardiology.

Research paper thumbnail of Toward detection of conduction tissue during cardiac surgery: Light at the end of the tunnel?

Heart Rhythm, 2020

Postoperative conduction block requiring lifetime pacemaker placement continues to be a considera... more Postoperative conduction block requiring lifetime pacemaker placement continues to be a considerable source of morbidity for patients undergoing repair of congenital heart defects. Damage to the cardiac conduction system (CCS) during surgical procedures is thought to be a major cause of the conduction block. Intraoperative identification and avoidance of the CCS is thus a key strategy to improve surgical outcomes. In order to avoid conduction tissue damage and mitigate morbidity a number of approaches have been developed. Here, we review the historical and contemporary approaches for identification of conduction tissue during cardiac surgery. The established approach for intraoperative identification is based on anatomical landmarks established in extensive histological studies of the normal and diseased heart. We focus on landmarks to identify the sinus and atrioventricular nodes during cardiac surgery. We also review technologies explored for intraoperative tissue identification including electrical impedance measurements and electrocardiography. We describe new optical approaches, in particular, and optical spectroscopy and fiberoptic confocal microscopy (FCM), for identification of CCS regions and working myocardium during surgery. As a template for translation of future technology developments, we describe research and regulatory pathways to translate FCM for cardiac surgery. We suggest that along with more robust approaches to surgeon training including awareness of fundamental anatomical studies, optical approaches such as FCM show promise in aiding surgeons in repairs of heart defects. In particular, for complex defects, these approaches can complement landmarkbased identification of conduction tissue and thus help to avoid injury to the CCS due to surgical procedures.

Research paper thumbnail of Pediatric Airway Stent Designed to Facilitate Mucus Transport and Atraumatic Removal

IEEE Transactions on Biomedical Engineering, 2020

Research paper thumbnail of Active pelvic tilt is reduced in athletes with groin injury; a case-controlled study

Physical Therapy in Sport, 2018

OBJECTIVE To study if athletes with groin injury had less active pelvic tilt (APT) than non-injur... more OBJECTIVE To study if athletes with groin injury had less active pelvic tilt (APT) than non-injured controls. DESIGN Case-control. SETTING Sports physiotherapy clinics and sports clubs. PARTICIPANTS 17 athletes (Tegner>5, age 25.1(5.2) with groin injury and 27 healthy controls (Tenger>5, age 24.4(3.6)). MAIN OUTCOME MEASURES Active pelvic tilt, defining the ability of an individual to actively tilt the pelvis anteriorly and posteriorly over a frontal axis, and hip range of motion (HROM) parameters. RESULTS Linear regression model associations with generalized estimated equations revealed that APT was lower on injured sides compared to non-injured for total (21.1(7.1) vs. 27.2(8.0), P = .003, effect size (ES) = 0.8) and anterior (10.2(5.9) vs. 13.7(4.8), P = .004, ES = 0.65) APT. Posterior APT (-10.9(3.6) vs. -13.4(5.2), P = .06, ES = 0.56) showed a trend towards being lower in those with groin injury. HROM parameters were not found associated. CONCLUSIONS Total active and anterior pelvic tilt were lower on the injured side in athletes with groin injury when compared to non-injured sides and healthy controls. This may be a relevant factor to consider in rehabilitation. Whether this is a cause or effect cannot be ascertained due to the cross sectional study design.

Research paper thumbnail of Substitution of iron with cobalt in the prosthetic group of bacterial cytochrome P450: Effects on the stability and structure of the protein

Inorganica Chimica Acta, 2018

Abstract The origin of thermostability of the heme monooxygenase from Thermus thermophilus, CYP17... more Abstract The origin of thermostability of the heme monooxygenase from Thermus thermophilus, CYP175A1 has been investigated using multi-wavelength circular dichroism (CD) spectroscopy at different temperatures. The far-UV and visible CD studies were carried out on WTCYP175A1, reduced WTCYP175A1 (i.e. Fe(II)-WTCYP175A1), apoCYP175A1, and the cobalt protoporphyrin (CoP) bound apoprotein (CoPCYP175A1) to determine the thermostability of the secondary and tertiary structure of the variants of the enzyme. Non-linear least squares analyses of the multi-wavelength CD data were used to determine the midpoint unfolding temperature (Tm), and the associated thermodynamic parameters for unfolding of the enzyme. The midpoint temperature (Tm) for thermal unfolding of WTCYP175A1 was much higher than that of the reduced enzyme (Fe(II)-WTCYP175A1), which was higher than that of the CoP bound analogue, while the Tm for the apoprotein was even lower than the CoPCYP175A1. The temperature for maximum stability (TS) also followed the order WTCYP175A1 > Fe(II)-WTCYP175A1 ≈ CoPCYP175A1 > apoCYP175A1 supporting that the binding of the metal prosthetic group indeed have important role on the overall stability of the protein. However, the maximum free energy of unfolding (ΔGS) of the secondary structure of the protein remained almost the same for all variants of CYP175A1. These studies thus show that weak non-bonding interactions such as hydrogen bonding and metal coordination play critical role in imparting high thermostability of the overall structure of the thermostable enzyme, CYP175A1. The thermostability of tertiary structure of the active site of the enzyme was also shown to depend on the nature of binding of the metal cofactor to the protein matrix.

Research paper thumbnail of Characterization, genetic regulation and production of cyanobacterial exopolysaccharides and its applicability for heavy metal removal

Carbohydrate polymers, 2018

Cyanobacteria are uniquely suited for the development of sustainable bioproduction platforms but ... more Cyanobacteria are uniquely suited for the development of sustainable bioproduction platforms but are currently underutilized due to lack of genetic tools. Exopolysaccharide (EPS) is of significant biotechnological importance due to their technological application in various industries. It has been found that most of the research works are focused on isolation and characterization of new exopolysaccharides from microbial sources. The exopolysaccharides from cyanobacteria have been poorly explored despite their original structural features associated with specific biological and physicochemical properties. However, it could increase in a near future through the use of inexpensive cyanobacterial platform as well as available information on the structural data and specific properties of these biopolymers. This review covers genetic regulation for production of exopolysaccharide, analytical strategies for their characterization, evaluation of structure property relationship and design of...

Research paper thumbnail of Isolation, screening and characterization of a novel extracellular xylanase from Aspergillus niger (KP874102.1) and its application in orange peel hydrolysis

International journal of biological macromolecules, Jan 12, 2017

In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was... more In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was isolated through cultural and morphological observations from soil sample of Baramura forest, Tripura west, India. 28S rDNA technique was applied for genomic identification of this fungal strain. The isolated strain was found to be phylogenetically closely related to Aspergillus niger. Kinetic constants such as Km and Vmax for extracellular xylanase were determined using various substrate such as beech wood xylan, oat spelt xylan and CM cellulose through Lineweaver-Burk plot. Km, Vmax and Kcat for beech wood xylan are found to be 2.89mg/ml, 2442U and 426178Umlmg(-1) respectively. Crude enzyme did not show also CM cellulose activity. The relative efficiency of oat spelt xylan was found to be 0.819 with respect to beech wood xylan. After acid hydrolysis, enzyme was able to produce reducing sugar with 17.7, 35.5, 50.8 and 65% (w/w) from orange peel after 15, 30, 45 and 60min incubation wit...

Research paper thumbnail of Computational simulations of asymmetric fluxes of large molecules through gap junction channel pores

Journal of Theoretical Biology, 2017

Gap junction channels are formed out of connexin isoforms, which enable molecule and ion selectiv... more Gap junction channels are formed out of connexin isoforms, which enable molecule and ion selective diffusion amongst neighboring cells. HeLa cells expressing distinct connexins (Cx) allow the formation of heterotypic channels, where we observed a molecular charge-independent preferential flux of large fluorescent molecules in the Cx45 to Cx43 direction. We hypothesize that the pore's shape is a significant factor along-side charge and transjunctional voltages for this asymmetric flux. To test this hypothesis, we developed a 3D computational model simulating Brownian diffusion of large molecules in a gap junction channel pore. The basic pore contour was derived from x-ray crystallographic structures of Cx43 and Cx26 and approximated using basic geometric shapes. Lucifer yellow dye molecules and cesium counter-ions were modeled as spheres using their respective Stokes radii. Our simulation results from simple diffusion and constant concentration gradient experiments showed that only charged particles yield asymmetric fluxes in heterotypic pores. While increasing the inner mouth size resulted in a near-quadratic rise in flux, the rise was asymptotic for outer mouth radii increase. Probability maps and average force per particle per pore section explain the asymmetric flux with variation in pore shape. Furthermore, the simulation results are in agreement with our in vitro experimental results with HeLa cells in Cx43-Cx45 heterotypic configurations. The presence of asymmetric fluxes can help us to understand effects of the molecular structure of the pore and predict potential differences in vivo.

Research paper thumbnail of Brownian Permeability Computation Model Predicts That Differences in the Internal Radii of the Pore are Determinant for Unidirectional and Reversal Fluxes through Gap Junction Channels

Biophysical Journal, 2012

trafficking of Kv channels to and from the cell surface. TIRF-based studies indicated that GFP-Kv... more trafficking of Kv channels to and from the cell surface. TIRF-based studies indicated that GFP-Kv2.1 containing vesicles tether directly to and deliver cargo in a discrete fashion to the Kv2.1 surface clusters in both transfected HEK and cultured hippocampal neurons. Qdot-based single molecule experiments indicated that the delivery and surface retrieval of Kv2.1 occurs at the perimeter of the surface clusters. Overall, 8558.4% of newly synthesized channels in HEK cells and 84.9510.4% in hippocampal neurons were inserted at the cluster perimeter even though the Kv2.1 clusters represent only 21.4 53.8% of the basal cell surface. When 132 continuously recycling Kv2.1 channels in HEK cells were examined, 96.2% were also inserted at the cluster perimeter. The actin depolymerizing agents swinholide A and cytochalasin D reduced the cluster localized insertion to 5 and 0% of control, respectively. Unlike Kv2.1, the Kv1.4 Kþ channel has a homogeneous cell-surface expression in transfected cells. Demonstrating that Kv2.1 clusters represent cell-surface platforms for more than just Kv2.1 insertion and retrieval, the non-clustering Kv1.4 Kþ channel also inserted into the HEK cell plasma membrane at the Kv2.1 cluster perimeter. Kv1.4 endocytosis also occurred at this region. Together, these results indicate that a non-conducting function of Kv2.1 is to form specialized cell-surface microdomains which are involved in ion channel trafficking. These domains may also be involved in additional cellular processes.

Research paper thumbnail of Heteromultimeric Gap-Junction Channel Permeance: Directional Fluxes Simulated Using a Brownian Dynamics Model

Biophysical Journal, 2010

20% in nominally zero Ca o (n=5). This Ca 2þ-dependent uncoupling was demonstrated to be CaM-depe... more 20% in nominally zero Ca o (n=5). This Ca 2þ-dependent uncoupling was demonstrated to be CaM-dependent by acute (10-15 min) pretreatment with 2 mM calmidazolium, wherein Cx43 g j declined by < 10% within 10 min (n=4). To directly test for the involvement of the Cx43 amino acid residue #136-158 domain in this Ca 2þ /CaM-dependent gap junction uncoupling process, 1 mM peptides were added to both whole cell patch pipettes and the 1 mM ionomycin/1.8 mM Ca o perfusion experiments were repeated. The Cx43 #136-158 sequence mimetic peptide (K d (CaM) = 860 nM) effectively prevented the Cx43 g j decline (< 3%, n=4) whereas a scrambled sequence peptide control failed to prevent the Ca 2þ-induced rundown of Cx43 g j (<90%, n=3). These data unequivocally demonstrate that influx of external Ca 2þ induces closure of Cx43 gap junctions in a CaM-dependent process involving the Cx43 residue #136-158 CL domain. This process has significant implications for the modulation of cardiac g j by Ca i and the ''healing-over'' of infarcted myocardium.

Research paper thumbnail of ge의 지배구조와 국제조세전략

Korean Accounting Association, Aug 1, 2020

Research paper thumbnail of Cyanobacteria: A Biocatalyst in Microbial Fuel Cell for Sustainable Electricity Generation

Waste to Sustainable Energy, 2019

Research paper thumbnail of Effects of graphene on thermal properties and thermal stability of polycarbonate/graphene nanocomposite

Journal of Thermoplastic Composite Materials, 2021

Polycarbonate (PC) /graphene nanocomposite was prepared using multilayer graphene (MLG) with load... more Polycarbonate (PC) /graphene nanocomposite was prepared using multilayer graphene (MLG) with loadings of 0.5, 1, and 3 wt% via melt mixing process. Morphological, structural, and thermal properties of the PC/MLG nanocomposites are investigated to look into the influence of MLG on the nanocomposite. A significant increase (∼6.4°C) in glass transition temperature is observed upon the addition of 3 wt% of MLG into the polycarbonate matrix. This increase in glass transition temperature may be due to the interaction between the MLG and polycarbonate polymer matrix. The specific heat capacity of pure PC and PC/MLG nanocomposites varies linearly with temperature below their glass transition. Upon the addition of MLGs, the overall thermal stability of PC/MLG nanocomposites increases with MLG loadings. A maximum increase about 29.23°C in T onset of thermal decomposition is observed in PC/MLG nanocomposite having 3 wt% of MLG loading. The activation energy ( Ea) of thermal decomposition is al...

Research paper thumbnail of Federated Adaptive Bitrate Live Streaming over Locality Sensitive Playback Coalitions

Live video broadcasts to particular communities or targeted audiences many-at-a-times indulge a c... more Live video broadcasts to particular communities or targeted audiences many-at-a-times indulge a cluster of localities from where the end-users are interested in participating. In this paper, we leverage this idea to develop a system called Federated Live Streaming over DASH (FLiDASH) which forms end-users coalitions based on the locality of the network connectivity (like a set of end-users behind a common core-network service gateway). In FLiDASH, the coalition members stream the live data collectively based on a federated adaptive bitrate streaming mechanism where the coalition as a whole decides the optimal bitrate for video streaming and distributes the download overhead among its members. We have thoroughly evaluated FLiDASH in an emulated setup, and observe a 40% improvement in the live streaming QoE with a 20% reduction in the network traffic usage.

Research paper thumbnail of Design of Grid Connected H Bridge Multi Level Inverter with PV System

This paper describes the various topologies and controlling strategies used in multi level invert... more This paper describes the various topologies and controlling strategies used in multi level inverter. MLI are used to improve the waveform output of the inverter to be nearly to sinusoidal so that the harmonic injection by that inverter is minimum. In this paper implement a multi level inverter used in photo voltaic grid integration. A cascaded asymmetric multi level inverter is proposed which contains minimum no. of switches and it can be employed in AC application using solar energy. The proposed topology consists of 25 output level using 10 switches with nearly sinusoidal output, thereby reducing gate driver circuit and layout of optimizing circuit. All the simulation are carried out by using MATLAB 2010a.

Research paper thumbnail of Toward cardiac tissue characterization using machine learning and light-scattering spectroscopy

Journal of Biomedical Optics, 2021

Research paper thumbnail of An ns3-based Energy Module of 5G NR User Equipments for Millimeter Wave Networks

IEEE INFOCOM 2021 - IEEE Conference on Computer Communications Workshops (INFOCOM WKSHPS), 2021

This poster presents the design, development and test results of an energy consumption analysis m... more This poster presents the design, development and test results of an energy consumption analysis module developed over ns3 Millimeter Wave (mmWave) communication for analyzing power consumption for 5G New Radio (NR) User Equipment (UE) during both continuous and discontinuous packet receptions. This module is important to analyze and explore the energy consumption behavior of the 5G communication protocols under the NR technology. The developed module includes the complete Radio Resource Control (RRC) state machine for 5G NR recommended by 3GPP Specification 38.840. To the best of our knowledge, the designed module is the first of its kind that provides a comprehensive energy analysis for the 5G NR UEs over mmWave communication.

Research paper thumbnail of System call interception for serverless isolation

Proceedings of the SIGCOMM '20 Poster and Demo Sessions, 2020

Research paper thumbnail of Multi-Label Classification of Parrott’s Emotions

Proceedings of the 33rd International Conference on Software Engineering and Knowledge Engineering, 2021

Mining for latent emotions embedded in tweets can offer clues about users' affective state on a b... more Mining for latent emotions embedded in tweets can offer clues about users' affective state on a broad range of topics ranging from their mental health to political opinions. This paper presents a multi-class supervised learning approach to group tweets into six emotions (joy, sadness, anger, fear, love, and surprise) defined according to the Parrott's framework. After extensive pre-processing, linguistic and metadata features extracted from a corpus of tweets are used to train popular machine learning classifiers. The performance of these classifiers is evaluated using accuracy, sensitivity, and specificity computed based on a multi-class confusion matrix approach. Our framework can detect common emotions of joy and sadness with excellent accuracy (> 90%), anger and fear with moderate accuracy (75% − 85%), and love and surprise with lower accuracy (50% − 60%). Overall, the accuracy of our framework still outperforms that of contemporary approaches for all the six emotions. Further analysis of an example multi-class confusion matrix indicates that lower accuracy values for love and surprise may arise because love is often confused with joy, whereas surprise is mixed up with the positive emotion of joy and the negative emotion of fear. Moreover, this confusion could be attributed to an under-representation of these emotions in the data. This highlights the need for building high-quality, balanced benchmark data sets for training multi-label emotion classifiers.

Research paper thumbnail of Effect of Pleurotus ostreatus (Jacq.) and Trichoderma harzianum (Rifai) on Meloidogyne incognita (Kofoid White) in tomato (Solanum lycopersicum Mill.)

Acta Scientiarum. Biological Sciences, 2020

Two isolations of fungi from a bank of microorganisms in the Biological Sciences Laboratory at Es... more Two isolations of fungi from a bank of microorganisms in the Biological Sciences Laboratory at Escuela Superior Politécnica de Chimborazo were tested on the galling caused by Meloidogyne incognita in tomato seedlings grown in pots with substrate infested with a suspension of nematodes, with approximately 2000 juvenile stages (J2) from root galls of plants infested with M. incognita, taken from the Nematology laboratory of the Ecuadorian Agricultural Quality Assurance Agency (AGROCALIDAD). Pleurotus ostreatus was a fungus with nematicidal characteristics through production of toxins; while Trichoderma harzianum is a widely known fungus, although it is a plant growth promoter rather than a nematicide. The two fungi were formulated in wheat straw and rabbit manure. A complete randomized design (CRD) with three replications was used, with a chemical control (Fenamiphos) and an absolute control. Five grams of each formulation was applied per plant before the transplant. The number of g...

Research paper thumbnail of Localization of the sinoatrial and atrioventricular nodal region in neonatal and juvenile ovine hearts

PLOS ONE, 2020

Localization of the components of the cardiac conduction system (CCS) is essential for many thera... more Localization of the components of the cardiac conduction system (CCS) is essential for many therapeutic procedures in cardiac surgery and interventional cardiology. While histological studies provided fundamental insights into CCS localization, this information is incomplete and difficult to translate to aid in intraprocedural localization. To advance our understanding of CCS localization, we set out to establish a framework for quantifying nodal region morphology. Using this framework, we quantitatively analyzed the sinoatrial node (SAN) and atrioventricular node (AVN) in ovine with postmenstrual age ranging from 4.4 to 58.3 months. In particular, we studied the SAN and AVN in relation to the epicardial and endocardial surfaces, respectively. Using anatomical landmarks, we excised the nodes and adjacent tissues, sectioned those at a thickness of 4 μm at 100 μm intervals, and applied Masson's trichrome stain to the sections. These sections were then imaged, segmented to identify nodal tissue, and analyzed to quantify nodal depth and superficial tissue composition. The minimal SAN depth ranged between 20 and 926 μm. AVN minimal depth ranged between 59 and 1192 μm in the AVN extension region, 49 and 980 μm for the compact node, and 148 and 888 μm for the transition to His Bundle region. Using a logarithmic regression model, we found that minimal depth increased logarithmically with age for the AVN (R 2 = 0.818, P = 0.002). Also, the myocardial overlay of the AVN was heterogeneous within different regions and decreased with increasing age. Age associated alterations of SAN minimal depth were insignificant. Our study presents examples of characteristic tissue patterns superficial to the AVN and within the SAN. We suggest that the presented framework provides quantitative information for CCS localization. Our studies indicate that procedural methods and localization approaches in regions near the AVN should account for the age of patients in cardiac surgery and interventional cardiology.

Research paper thumbnail of Toward detection of conduction tissue during cardiac surgery: Light at the end of the tunnel?

Heart Rhythm, 2020

Postoperative conduction block requiring lifetime pacemaker placement continues to be a considera... more Postoperative conduction block requiring lifetime pacemaker placement continues to be a considerable source of morbidity for patients undergoing repair of congenital heart defects. Damage to the cardiac conduction system (CCS) during surgical procedures is thought to be a major cause of the conduction block. Intraoperative identification and avoidance of the CCS is thus a key strategy to improve surgical outcomes. In order to avoid conduction tissue damage and mitigate morbidity a number of approaches have been developed. Here, we review the historical and contemporary approaches for identification of conduction tissue during cardiac surgery. The established approach for intraoperative identification is based on anatomical landmarks established in extensive histological studies of the normal and diseased heart. We focus on landmarks to identify the sinus and atrioventricular nodes during cardiac surgery. We also review technologies explored for intraoperative tissue identification including electrical impedance measurements and electrocardiography. We describe new optical approaches, in particular, and optical spectroscopy and fiberoptic confocal microscopy (FCM), for identification of CCS regions and working myocardium during surgery. As a template for translation of future technology developments, we describe research and regulatory pathways to translate FCM for cardiac surgery. We suggest that along with more robust approaches to surgeon training including awareness of fundamental anatomical studies, optical approaches such as FCM show promise in aiding surgeons in repairs of heart defects. In particular, for complex defects, these approaches can complement landmarkbased identification of conduction tissue and thus help to avoid injury to the CCS due to surgical procedures.

Research paper thumbnail of Pediatric Airway Stent Designed to Facilitate Mucus Transport and Atraumatic Removal

IEEE Transactions on Biomedical Engineering, 2020

Research paper thumbnail of Active pelvic tilt is reduced in athletes with groin injury; a case-controlled study

Physical Therapy in Sport, 2018

OBJECTIVE To study if athletes with groin injury had less active pelvic tilt (APT) than non-injur... more OBJECTIVE To study if athletes with groin injury had less active pelvic tilt (APT) than non-injured controls. DESIGN Case-control. SETTING Sports physiotherapy clinics and sports clubs. PARTICIPANTS 17 athletes (Tegner>5, age 25.1(5.2) with groin injury and 27 healthy controls (Tenger>5, age 24.4(3.6)). MAIN OUTCOME MEASURES Active pelvic tilt, defining the ability of an individual to actively tilt the pelvis anteriorly and posteriorly over a frontal axis, and hip range of motion (HROM) parameters. RESULTS Linear regression model associations with generalized estimated equations revealed that APT was lower on injured sides compared to non-injured for total (21.1(7.1) vs. 27.2(8.0), P = .003, effect size (ES) = 0.8) and anterior (10.2(5.9) vs. 13.7(4.8), P = .004, ES = 0.65) APT. Posterior APT (-10.9(3.6) vs. -13.4(5.2), P = .06, ES = 0.56) showed a trend towards being lower in those with groin injury. HROM parameters were not found associated. CONCLUSIONS Total active and anterior pelvic tilt were lower on the injured side in athletes with groin injury when compared to non-injured sides and healthy controls. This may be a relevant factor to consider in rehabilitation. Whether this is a cause or effect cannot be ascertained due to the cross sectional study design.

Research paper thumbnail of Substitution of iron with cobalt in the prosthetic group of bacterial cytochrome P450: Effects on the stability and structure of the protein

Inorganica Chimica Acta, 2018

Abstract The origin of thermostability of the heme monooxygenase from Thermus thermophilus, CYP17... more Abstract The origin of thermostability of the heme monooxygenase from Thermus thermophilus, CYP175A1 has been investigated using multi-wavelength circular dichroism (CD) spectroscopy at different temperatures. The far-UV and visible CD studies were carried out on WTCYP175A1, reduced WTCYP175A1 (i.e. Fe(II)-WTCYP175A1), apoCYP175A1, and the cobalt protoporphyrin (CoP) bound apoprotein (CoPCYP175A1) to determine the thermostability of the secondary and tertiary structure of the variants of the enzyme. Non-linear least squares analyses of the multi-wavelength CD data were used to determine the midpoint unfolding temperature (Tm), and the associated thermodynamic parameters for unfolding of the enzyme. The midpoint temperature (Tm) for thermal unfolding of WTCYP175A1 was much higher than that of the reduced enzyme (Fe(II)-WTCYP175A1), which was higher than that of the CoP bound analogue, while the Tm for the apoprotein was even lower than the CoPCYP175A1. The temperature for maximum stability (TS) also followed the order WTCYP175A1 > Fe(II)-WTCYP175A1 ≈ CoPCYP175A1 > apoCYP175A1 supporting that the binding of the metal prosthetic group indeed have important role on the overall stability of the protein. However, the maximum free energy of unfolding (ΔGS) of the secondary structure of the protein remained almost the same for all variants of CYP175A1. These studies thus show that weak non-bonding interactions such as hydrogen bonding and metal coordination play critical role in imparting high thermostability of the overall structure of the thermostable enzyme, CYP175A1. The thermostability of tertiary structure of the active site of the enzyme was also shown to depend on the nature of binding of the metal cofactor to the protein matrix.

Research paper thumbnail of Characterization, genetic regulation and production of cyanobacterial exopolysaccharides and its applicability for heavy metal removal

Carbohydrate polymers, 2018

Cyanobacteria are uniquely suited for the development of sustainable bioproduction platforms but ... more Cyanobacteria are uniquely suited for the development of sustainable bioproduction platforms but are currently underutilized due to lack of genetic tools. Exopolysaccharide (EPS) is of significant biotechnological importance due to their technological application in various industries. It has been found that most of the research works are focused on isolation and characterization of new exopolysaccharides from microbial sources. The exopolysaccharides from cyanobacteria have been poorly explored despite their original structural features associated with specific biological and physicochemical properties. However, it could increase in a near future through the use of inexpensive cyanobacterial platform as well as available information on the structural data and specific properties of these biopolymers. This review covers genetic regulation for production of exopolysaccharide, analytical strategies for their characterization, evaluation of structure property relationship and design of...

Research paper thumbnail of Isolation, screening and characterization of a novel extracellular xylanase from Aspergillus niger (KP874102.1) and its application in orange peel hydrolysis

International journal of biological macromolecules, Jan 12, 2017

In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was... more In the present work, a potent xylanase producing fungal strain Aspergillus niger (KP874102.1) was isolated through cultural and morphological observations from soil sample of Baramura forest, Tripura west, India. 28S rDNA technique was applied for genomic identification of this fungal strain. The isolated strain was found to be phylogenetically closely related to Aspergillus niger. Kinetic constants such as Km and Vmax for extracellular xylanase were determined using various substrate such as beech wood xylan, oat spelt xylan and CM cellulose through Lineweaver-Burk plot. Km, Vmax and Kcat for beech wood xylan are found to be 2.89mg/ml, 2442U and 426178Umlmg(-1) respectively. Crude enzyme did not show also CM cellulose activity. The relative efficiency of oat spelt xylan was found to be 0.819 with respect to beech wood xylan. After acid hydrolysis, enzyme was able to produce reducing sugar with 17.7, 35.5, 50.8 and 65% (w/w) from orange peel after 15, 30, 45 and 60min incubation wit...

Research paper thumbnail of Computational simulations of asymmetric fluxes of large molecules through gap junction channel pores

Journal of Theoretical Biology, 2017

Gap junction channels are formed out of connexin isoforms, which enable molecule and ion selectiv... more Gap junction channels are formed out of connexin isoforms, which enable molecule and ion selective diffusion amongst neighboring cells. HeLa cells expressing distinct connexins (Cx) allow the formation of heterotypic channels, where we observed a molecular charge-independent preferential flux of large fluorescent molecules in the Cx45 to Cx43 direction. We hypothesize that the pore&amp;amp;amp;amp;amp;amp;#39;s shape is a significant factor along-side charge and transjunctional voltages for this asymmetric flux. To test this hypothesis, we developed a 3D computational model simulating Brownian diffusion of large molecules in a gap junction channel pore. The basic pore contour was derived from x-ray crystallographic structures of Cx43 and Cx26 and approximated using basic geometric shapes. Lucifer yellow dye molecules and cesium counter-ions were modeled as spheres using their respective Stokes radii. Our simulation results from simple diffusion and constant concentration gradient experiments showed that only charged particles yield asymmetric fluxes in heterotypic pores. While increasing the inner mouth size resulted in a near-quadratic rise in flux, the rise was asymptotic for outer mouth radii increase. Probability maps and average force per particle per pore section explain the asymmetric flux with variation in pore shape. Furthermore, the simulation results are in agreement with our in vitro experimental results with HeLa cells in Cx43-Cx45 heterotypic configurations. The presence of asymmetric fluxes can help us to understand effects of the molecular structure of the pore and predict potential differences in vivo.

Research paper thumbnail of Brownian Permeability Computation Model Predicts That Differences in the Internal Radii of the Pore are Determinant for Unidirectional and Reversal Fluxes through Gap Junction Channels

Biophysical Journal, 2012

trafficking of Kv channels to and from the cell surface. TIRF-based studies indicated that GFP-Kv... more trafficking of Kv channels to and from the cell surface. TIRF-based studies indicated that GFP-Kv2.1 containing vesicles tether directly to and deliver cargo in a discrete fashion to the Kv2.1 surface clusters in both transfected HEK and cultured hippocampal neurons. Qdot-based single molecule experiments indicated that the delivery and surface retrieval of Kv2.1 occurs at the perimeter of the surface clusters. Overall, 8558.4% of newly synthesized channels in HEK cells and 84.9510.4% in hippocampal neurons were inserted at the cluster perimeter even though the Kv2.1 clusters represent only 21.4 53.8% of the basal cell surface. When 132 continuously recycling Kv2.1 channels in HEK cells were examined, 96.2% were also inserted at the cluster perimeter. The actin depolymerizing agents swinholide A and cytochalasin D reduced the cluster localized insertion to 5 and 0% of control, respectively. Unlike Kv2.1, the Kv1.4 Kþ channel has a homogeneous cell-surface expression in transfected cells. Demonstrating that Kv2.1 clusters represent cell-surface platforms for more than just Kv2.1 insertion and retrieval, the non-clustering Kv1.4 Kþ channel also inserted into the HEK cell plasma membrane at the Kv2.1 cluster perimeter. Kv1.4 endocytosis also occurred at this region. Together, these results indicate that a non-conducting function of Kv2.1 is to form specialized cell-surface microdomains which are involved in ion channel trafficking. These domains may also be involved in additional cellular processes.

Research paper thumbnail of Heteromultimeric Gap-Junction Channel Permeance: Directional Fluxes Simulated Using a Brownian Dynamics Model

Biophysical Journal, 2010

20% in nominally zero Ca o (n=5). This Ca 2þ-dependent uncoupling was demonstrated to be CaM-depe... more 20% in nominally zero Ca o (n=5). This Ca 2þ-dependent uncoupling was demonstrated to be CaM-dependent by acute (10-15 min) pretreatment with 2 mM calmidazolium, wherein Cx43 g j declined by < 10% within 10 min (n=4). To directly test for the involvement of the Cx43 amino acid residue #136-158 domain in this Ca 2þ /CaM-dependent gap junction uncoupling process, 1 mM peptides were added to both whole cell patch pipettes and the 1 mM ionomycin/1.8 mM Ca o perfusion experiments were repeated. The Cx43 #136-158 sequence mimetic peptide (K d (CaM) = 860 nM) effectively prevented the Cx43 g j decline (< 3%, n=4) whereas a scrambled sequence peptide control failed to prevent the Ca 2þ-induced rundown of Cx43 g j (<90%, n=3). These data unequivocally demonstrate that influx of external Ca 2þ induces closure of Cx43 gap junctions in a CaM-dependent process involving the Cx43 residue #136-158 CL domain. This process has significant implications for the modulation of cardiac g j by Ca i and the ''healing-over'' of infarcted myocardium.