Ahmed Reda - Academia.edu (original) (raw)
Papers by Ahmed Reda
Frontiers in Endocrinology, 2014
The Egyptian Heart Journal
Background COVID-19 pandemic is associated with high morbidity and mortality. Cardiovascular insu... more Background COVID-19 pandemic is associated with high morbidity and mortality. Cardiovascular insult is a leading cause of in-hospital mortality in COVID-19 patients, especially right ventricular (RV) dysfunction and massive pulmonary embolism. This study aims to assess short-term impact of COVID 19 infection on (RV) functions among hospitalized patients with moderate or severe illness using bed side trans-thoracic echocardiogram. This study was conducted in 3 isolation hospitals in Cairo, spanning over 3 months during the expected pandemic peak in Egypt in 2020. The study recruited 100 consecutive patients with moderate or severe COVID-19 infection. Four patients refused to participate in the study. Patients with pre-existing structural heart diseases were excluded. All patients underwent full history taking and clinical examination. Bed side echocardiography was done emphasizing on (RV), and (RA) dimensions, (LV) functions and pulmonary artery systolic pressure (PSAS). Cardiac biom...
The Journal of urology, Jan 26, 2016
We assessed the effect of tamsulosin as an adjunctive therapy after shock wave lithotripsy for pe... more We assessed the effect of tamsulosin as an adjunctive therapy after shock wave lithotripsy for pediatric single renal pelvic stones. A total of 120 children with a unilateral single renal pelvic stone were included in a prospective randomized, controlled study. All children were randomized to 2 equal groups. Group 1 received tamsulosin (0.01 mg/kg once daily) as adjunctive therapy after shock wave lithotripsy in addition to paracetamol while group 2 received paracetamol only. Stone clearance was defined as no renal stone fragments or fragments less than 3 mm and no pelvicalyceal system dilatation. Our study included 69 boys and 51 girls with a median age of 3.5 years and a median stone size of 1.2 cm. There was no statistically significant difference between groups 1 and 2 in stone or patient criteria. Of the children 99 (82.5%) achieved stone clearance after the first session, including 50 in group 1 and 49 in group 2. All children in each group were cleared of stones after the sec...
Journal of Medical Histology, 2017
Background and Objectives: Sarco(endo) plasmic reticulum Ca 2+-ATPase (SERCA2a) is the ATP-driven... more Background and Objectives: Sarco(endo) plasmic reticulum Ca 2+-ATPase (SERCA2a) is the ATP-driven pump that translocates Ca 2+ from the cytoplasm to the lumen of the sarcoplasmic reticulum. 25% of children and adolescents with type 1 diabetes will develop diastolic dysfunction that results in part from a reduction in the activity of SERCA2a. The present study aimed at investigating and comparing the therapeutic effect of Adipose Mesenchymal Stem Cells (AMSCs) with SERCA2a gene modified AMSCs in diabetes type 1 induced cardiomyopathy of adult male albino rat. Materials and Methods: Twenty-nine adult male albino rats were divided into: Donor group of 2 rats used to obtain AMSCs. Group I (Control group): 6 rats not exposed to diabetes induction. Group II (Diabetic group): 7 rats injected with streptozotocin (STZ) 50mg/Kg once. Group III ((AMSCs group): 7 rats injected with AMSCs following induction of diabetes. Group IV (SERCA2a Modified AMSCs Group): 7 rats injected with SERCA2a modified AMSCs, following induction of diabetes. All rats were sacrificed 8 weeks from start of experiment. Results: Morphological changes, indicating inflammation and degeneration, were found in the cardiac muscle of diabetic rats and regressed remarkably by AMSCs and SERCA2a modified AMSCs. The regression of morphological changes was confirmed by histological, immunohistochemical, morphometric and serological studies. Conclusion: The therapeutic effect of SERCA2a modified AMSCs in diabetes type 1 cardiomyopathy was more remarkable than that of AMSCs.
Al-Azhar Dental Journal for Girls
Purpose: Platelet rich fibrin (PRF) has been widely and successfully used in periodontal regenera... more Purpose: Platelet rich fibrin (PRF) has been widely and successfully used in periodontal regeneration. Injectable PRF (i-PRF) is a liquid form of PRF containing more leucocyte and growth factors. I-PRF was used with great success in management of gingival recession and implant therapy. So the aim of the study was to evaluate the benefit of adding i-PRF to xenograft in management of periodontal intraosseous defects. Subjects and Methods: Twenty four patients (age range 36-59 years), with stage III periodontitis with at least one intrabony defect, probing pocket depth > 5 mm and radiographic evidence of vertical bone loss, were enrolled in the present study. The patients were equally divided into two groups, the test group (i-PRF and xenograft) and the control group (xenograft only). Probing depth (PD), clinical attachment level (CAL.), bone defect depth and bone density were recorded at baseline and after 6 months. Results: The results showed a statistical significant reduction in PD, gain in CAL, bone fill and change in bone density from preoperative to 6 months in each group separately. There was a statistical significant difference between test and control groups with mean and standard deviation (SD) of PD reduction (4.2±0.4; 3.3±0.7) and CAL gain (3.8±0.4 ; 2.9±0.8) respectively in favor of the test group. Regarding bone fill, there was no statistical significant difference between both groups with mean and SD (2.6±0.7; 2±0.8) respectively. As for the increase in bone density, there was a statistical significant difference between both groups with mean and SD (17.5±4.9 ;10.9±6.8) respectively. Conclusion: We can conclude that adding i-PRF to bone grafting material might have a beneficial impact in treatment of periodontal intraosseous defects.
Journal of Elastomers & Plastics, 2012
Calneuron-1 and-2 are members of the neuronal calcium-binding protein family (nCaBP). They are tr... more Calneuron-1 and-2 are members of the neuronal calcium-binding protein family (nCaBP). They are transmembrane Calmodulin-like EF-hand Ca 2+-sensors, and a function in the control of Golgi-to-plasma membrane vesicle trafficking has been assigned to both proteins. In this paper, we describe the distribution of Calneuron-1 in rat and human brains. We show that Calneuron-1 is ubiquitously expressed in all brain regions examined. The protein is most abundant in Purkinje cells of the cerebellum and principal neurons of the cortex and limbic brain whereas no expression in glial cells is apparent. In addition, we identify two novel splice isoforms of Calneuron-1 with extended N-termini. These isoforms are particular abundant in the cerebellum. Taken together, these data set grounds for a better understanding of the cellular function of Calneurons.
Journal of Medical Histology, 2017
Background: Doxorubicin (DOX), is a highly efficient anti-neoplastic drug used for treatment of s... more Background: Doxorubicin (DOX), is a highly efficient anti-neoplastic drug used for treatment of solid tumors including breast cancer (BC). Resveratrol (RES) is considered an interesting molecule in hormone dependent cancer therapy. Stem cell activation may induce less cardiomyocyte apoptosis, enhanced angiogenesis and increased proliferation of cardiomyocytes. Materials and Methods: Twenty seven adult male albino rats, were divided into: control group including 6 rats, group II (DOX group) including 7 rats that received intraperitoneal (IP) injection with DOX 3 mg /kg 3 times a week for 2 weeks. Group III (RES post-treatment group) included 7 rats that were injected with DOX followed by 50 mg/Kg RES daily orally for another 2 weeks. Group IV (RES pretreatment group) included 7 rats that were injected with DOX and received concomitantly 50 mg/Kg RES daily orally for 2 weeks and left untreated for another 2 weeks. By the end of the 4 th week all animals were sacrificed. Cardiac muscle specimens were subjected to histological, immunohistochemical, morphometric and serological studies. Results: Myocardial injury appeared as obvious congestion, apoptosis, degeneration, increased collagen fibers content, obvious caspase3 expression and minimal proliferating cell nuclear antigen (PCNA) +ve and CD44 +ve expression in DOX group. Regression of the previous changes was more remarkable in group IV than in group III except for PCNA and CD44 expression that was more obvious in both treatment groups. Conclusion: RES proved amelioration of DOX induced myocardial injury that was more noticeable in RES early administration. RES post-treatment might have been more efficient if longer duration was applied.
International journal of stem cells, Jan 31, 2018
Insulin secretion entirely depends on Ca influx and sequestration into endoplasmic reticulum (ER)... more Insulin secretion entirely depends on Ca influx and sequestration into endoplasmic reticulum (ER) of β-cells, performed by . In diabetes, SERCA2b is decreased in the β-cells leading to impaired intracellular Ca homeostasis and insulin secretion. Adipose mesenchymal stem cells (AMSCs) play a potential role in transplantation in animal models. The present study aimed at investigating and comparing the therapeutic effect of non-transfected AMSCs and gene transfected AMSCs on the pancreas of induced diabetes type 1 in rat. 58 adult male albino rats were divided into: Donor group: 22 rats, 2 for isolation, propagation and characterization of AMSCs and SERCA2b transfected AMSCs, in addition 20 for isolated islet calcium level assessment. Group I (Control Group): 6 rats, Group II (Diabetic Group): 10 rats, 50 mg streptozotocin (STZ) were injected intraperitoneal (IP), Group III (AMSCs Group): 10 rats, 1×10 AMSCs were injected intravenous and Group IV (SERCA2b transfected AMSCs Group): 10 r...
Open Access Macedonian Journal of Medical Sciences
BACKGROUND: Various attempts have been implemented using different materials and techniques to au... more BACKGROUND: Various attempts have been implemented using different materials and techniques to augment the maxillary sinus floor for prospect dental implant positioning.AIM: This contemplate was conducted to assess the osteogenic capability of the maxillary sinus in a two-step sinus membrane elevation using titanium mesh to keep the formed space to place dental implants in atrophic ridges.MATERIALS AND METHODS: Titanium micromesh was customized and positioned into the sinus on one side to preserve the elevated membrane in position. On the other side xenograft was applied. Instant and 6-months postoperative cone beam computed tomography (CBCT) was done to assess the gained bone height and density. Bone core biopsies were obtained during implant placement for histological and histomorphometric evaluation.RESULTS: The average bone height values increased in both groups. Meanwhile the average bone density value was higher at the graft group than the titanium mesh group. Histological and...
Vibrio species are major seafood-borne pathogens that causing gastroenteritis as a serious diseas... more Vibrio species are major seafood-borne pathogens that causing gastroenteritis as a serious disease of human public health due to consumption of raw unprepared or undercooked fish. A total of 80 fish samples (Nile tilapia, Nile perch, Grey mullet and Sea bass) (20 of each) were randomly collected from local market at Zagazig city to investigate the prevalence of Vibrio species in local marketed fish. The results revealed that Vibrio species isolated with a percentage of 13% in Nile tilapia, 80% in Nile perch, 73% in Grey mullet and 47% in Sea bass with an overall percentage of 53% of all examined fish. The isolated Vibrio species were 41% V. parahaemolyticus, 25% V. alginolyticus, 22% V.mimicus, 9% V.vulnificus and 3% V.cholera. The antimicrobial resistance patterns of isolated Vibrio species revealed that most vibrio isolates were resistance to Ampicillin (100%), Nalidexic acid (88.3%), Streptomycin (84.2%), Sulphamethoxazol (70.7%) and Oxytetracycline (64.8%) and the sensitivity to Amikacin (94%), Ciprofloxacin (70.5%), Gentamicin (58.9%). Dipping of tilapia in lemon juice 5% for 30 min reduced Vibrio parahaemolyticus count by 0.31log cfu/g (45.88%). In conclusion, our results confirm contamination of fish by antibiotic resistant vibrio. Dipping of fish in lemon juice is an efficient strategy in reducing Vibrio parahaemolyticus load in fish.
Frontiers in Endocrinology, 2014
The Egyptian Heart Journal
Background COVID-19 pandemic is associated with high morbidity and mortality. Cardiovascular insu... more Background COVID-19 pandemic is associated with high morbidity and mortality. Cardiovascular insult is a leading cause of in-hospital mortality in COVID-19 patients, especially right ventricular (RV) dysfunction and massive pulmonary embolism. This study aims to assess short-term impact of COVID 19 infection on (RV) functions among hospitalized patients with moderate or severe illness using bed side trans-thoracic echocardiogram. This study was conducted in 3 isolation hospitals in Cairo, spanning over 3 months during the expected pandemic peak in Egypt in 2020. The study recruited 100 consecutive patients with moderate or severe COVID-19 infection. Four patients refused to participate in the study. Patients with pre-existing structural heart diseases were excluded. All patients underwent full history taking and clinical examination. Bed side echocardiography was done emphasizing on (RV), and (RA) dimensions, (LV) functions and pulmonary artery systolic pressure (PSAS). Cardiac biom...
The Journal of urology, Jan 26, 2016
We assessed the effect of tamsulosin as an adjunctive therapy after shock wave lithotripsy for pe... more We assessed the effect of tamsulosin as an adjunctive therapy after shock wave lithotripsy for pediatric single renal pelvic stones. A total of 120 children with a unilateral single renal pelvic stone were included in a prospective randomized, controlled study. All children were randomized to 2 equal groups. Group 1 received tamsulosin (0.01 mg/kg once daily) as adjunctive therapy after shock wave lithotripsy in addition to paracetamol while group 2 received paracetamol only. Stone clearance was defined as no renal stone fragments or fragments less than 3 mm and no pelvicalyceal system dilatation. Our study included 69 boys and 51 girls with a median age of 3.5 years and a median stone size of 1.2 cm. There was no statistically significant difference between groups 1 and 2 in stone or patient criteria. Of the children 99 (82.5%) achieved stone clearance after the first session, including 50 in group 1 and 49 in group 2. All children in each group were cleared of stones after the sec...
Journal of Medical Histology, 2017
Background and Objectives: Sarco(endo) plasmic reticulum Ca 2+-ATPase (SERCA2a) is the ATP-driven... more Background and Objectives: Sarco(endo) plasmic reticulum Ca 2+-ATPase (SERCA2a) is the ATP-driven pump that translocates Ca 2+ from the cytoplasm to the lumen of the sarcoplasmic reticulum. 25% of children and adolescents with type 1 diabetes will develop diastolic dysfunction that results in part from a reduction in the activity of SERCA2a. The present study aimed at investigating and comparing the therapeutic effect of Adipose Mesenchymal Stem Cells (AMSCs) with SERCA2a gene modified AMSCs in diabetes type 1 induced cardiomyopathy of adult male albino rat. Materials and Methods: Twenty-nine adult male albino rats were divided into: Donor group of 2 rats used to obtain AMSCs. Group I (Control group): 6 rats not exposed to diabetes induction. Group II (Diabetic group): 7 rats injected with streptozotocin (STZ) 50mg/Kg once. Group III ((AMSCs group): 7 rats injected with AMSCs following induction of diabetes. Group IV (SERCA2a Modified AMSCs Group): 7 rats injected with SERCA2a modified AMSCs, following induction of diabetes. All rats were sacrificed 8 weeks from start of experiment. Results: Morphological changes, indicating inflammation and degeneration, were found in the cardiac muscle of diabetic rats and regressed remarkably by AMSCs and SERCA2a modified AMSCs. The regression of morphological changes was confirmed by histological, immunohistochemical, morphometric and serological studies. Conclusion: The therapeutic effect of SERCA2a modified AMSCs in diabetes type 1 cardiomyopathy was more remarkable than that of AMSCs.
Al-Azhar Dental Journal for Girls
Purpose: Platelet rich fibrin (PRF) has been widely and successfully used in periodontal regenera... more Purpose: Platelet rich fibrin (PRF) has been widely and successfully used in periodontal regeneration. Injectable PRF (i-PRF) is a liquid form of PRF containing more leucocyte and growth factors. I-PRF was used with great success in management of gingival recession and implant therapy. So the aim of the study was to evaluate the benefit of adding i-PRF to xenograft in management of periodontal intraosseous defects. Subjects and Methods: Twenty four patients (age range 36-59 years), with stage III periodontitis with at least one intrabony defect, probing pocket depth > 5 mm and radiographic evidence of vertical bone loss, were enrolled in the present study. The patients were equally divided into two groups, the test group (i-PRF and xenograft) and the control group (xenograft only). Probing depth (PD), clinical attachment level (CAL.), bone defect depth and bone density were recorded at baseline and after 6 months. Results: The results showed a statistical significant reduction in PD, gain in CAL, bone fill and change in bone density from preoperative to 6 months in each group separately. There was a statistical significant difference between test and control groups with mean and standard deviation (SD) of PD reduction (4.2±0.4; 3.3±0.7) and CAL gain (3.8±0.4 ; 2.9±0.8) respectively in favor of the test group. Regarding bone fill, there was no statistical significant difference between both groups with mean and SD (2.6±0.7; 2±0.8) respectively. As for the increase in bone density, there was a statistical significant difference between both groups with mean and SD (17.5±4.9 ;10.9±6.8) respectively. Conclusion: We can conclude that adding i-PRF to bone grafting material might have a beneficial impact in treatment of periodontal intraosseous defects.
Journal of Elastomers & Plastics, 2012
Calneuron-1 and-2 are members of the neuronal calcium-binding protein family (nCaBP). They are tr... more Calneuron-1 and-2 are members of the neuronal calcium-binding protein family (nCaBP). They are transmembrane Calmodulin-like EF-hand Ca 2+-sensors, and a function in the control of Golgi-to-plasma membrane vesicle trafficking has been assigned to both proteins. In this paper, we describe the distribution of Calneuron-1 in rat and human brains. We show that Calneuron-1 is ubiquitously expressed in all brain regions examined. The protein is most abundant in Purkinje cells of the cerebellum and principal neurons of the cortex and limbic brain whereas no expression in glial cells is apparent. In addition, we identify two novel splice isoforms of Calneuron-1 with extended N-termini. These isoforms are particular abundant in the cerebellum. Taken together, these data set grounds for a better understanding of the cellular function of Calneurons.
Journal of Medical Histology, 2017
Background: Doxorubicin (DOX), is a highly efficient anti-neoplastic drug used for treatment of s... more Background: Doxorubicin (DOX), is a highly efficient anti-neoplastic drug used for treatment of solid tumors including breast cancer (BC). Resveratrol (RES) is considered an interesting molecule in hormone dependent cancer therapy. Stem cell activation may induce less cardiomyocyte apoptosis, enhanced angiogenesis and increased proliferation of cardiomyocytes. Materials and Methods: Twenty seven adult male albino rats, were divided into: control group including 6 rats, group II (DOX group) including 7 rats that received intraperitoneal (IP) injection with DOX 3 mg /kg 3 times a week for 2 weeks. Group III (RES post-treatment group) included 7 rats that were injected with DOX followed by 50 mg/Kg RES daily orally for another 2 weeks. Group IV (RES pretreatment group) included 7 rats that were injected with DOX and received concomitantly 50 mg/Kg RES daily orally for 2 weeks and left untreated for another 2 weeks. By the end of the 4 th week all animals were sacrificed. Cardiac muscle specimens were subjected to histological, immunohistochemical, morphometric and serological studies. Results: Myocardial injury appeared as obvious congestion, apoptosis, degeneration, increased collagen fibers content, obvious caspase3 expression and minimal proliferating cell nuclear antigen (PCNA) +ve and CD44 +ve expression in DOX group. Regression of the previous changes was more remarkable in group IV than in group III except for PCNA and CD44 expression that was more obvious in both treatment groups. Conclusion: RES proved amelioration of DOX induced myocardial injury that was more noticeable in RES early administration. RES post-treatment might have been more efficient if longer duration was applied.
International journal of stem cells, Jan 31, 2018
Insulin secretion entirely depends on Ca influx and sequestration into endoplasmic reticulum (ER)... more Insulin secretion entirely depends on Ca influx and sequestration into endoplasmic reticulum (ER) of β-cells, performed by . In diabetes, SERCA2b is decreased in the β-cells leading to impaired intracellular Ca homeostasis and insulin secretion. Adipose mesenchymal stem cells (AMSCs) play a potential role in transplantation in animal models. The present study aimed at investigating and comparing the therapeutic effect of non-transfected AMSCs and gene transfected AMSCs on the pancreas of induced diabetes type 1 in rat. 58 adult male albino rats were divided into: Donor group: 22 rats, 2 for isolation, propagation and characterization of AMSCs and SERCA2b transfected AMSCs, in addition 20 for isolated islet calcium level assessment. Group I (Control Group): 6 rats, Group II (Diabetic Group): 10 rats, 50 mg streptozotocin (STZ) were injected intraperitoneal (IP), Group III (AMSCs Group): 10 rats, 1×10 AMSCs were injected intravenous and Group IV (SERCA2b transfected AMSCs Group): 10 r...
Open Access Macedonian Journal of Medical Sciences
BACKGROUND: Various attempts have been implemented using different materials and techniques to au... more BACKGROUND: Various attempts have been implemented using different materials and techniques to augment the maxillary sinus floor for prospect dental implant positioning.AIM: This contemplate was conducted to assess the osteogenic capability of the maxillary sinus in a two-step sinus membrane elevation using titanium mesh to keep the formed space to place dental implants in atrophic ridges.MATERIALS AND METHODS: Titanium micromesh was customized and positioned into the sinus on one side to preserve the elevated membrane in position. On the other side xenograft was applied. Instant and 6-months postoperative cone beam computed tomography (CBCT) was done to assess the gained bone height and density. Bone core biopsies were obtained during implant placement for histological and histomorphometric evaluation.RESULTS: The average bone height values increased in both groups. Meanwhile the average bone density value was higher at the graft group than the titanium mesh group. Histological and...
Vibrio species are major seafood-borne pathogens that causing gastroenteritis as a serious diseas... more Vibrio species are major seafood-borne pathogens that causing gastroenteritis as a serious disease of human public health due to consumption of raw unprepared or undercooked fish. A total of 80 fish samples (Nile tilapia, Nile perch, Grey mullet and Sea bass) (20 of each) were randomly collected from local market at Zagazig city to investigate the prevalence of Vibrio species in local marketed fish. The results revealed that Vibrio species isolated with a percentage of 13% in Nile tilapia, 80% in Nile perch, 73% in Grey mullet and 47% in Sea bass with an overall percentage of 53% of all examined fish. The isolated Vibrio species were 41% V. parahaemolyticus, 25% V. alginolyticus, 22% V.mimicus, 9% V.vulnificus and 3% V.cholera. The antimicrobial resistance patterns of isolated Vibrio species revealed that most vibrio isolates were resistance to Ampicillin (100%), Nalidexic acid (88.3%), Streptomycin (84.2%), Sulphamethoxazol (70.7%) and Oxytetracycline (64.8%) and the sensitivity to Amikacin (94%), Ciprofloxacin (70.5%), Gentamicin (58.9%). Dipping of tilapia in lemon juice 5% for 30 min reduced Vibrio parahaemolyticus count by 0.31log cfu/g (45.88%). In conclusion, our results confirm contamination of fish by antibiotic resistant vibrio. Dipping of fish in lemon juice is an efficient strategy in reducing Vibrio parahaemolyticus load in fish.