Akash Sharma - Academia.edu (original) (raw)

Papers by Akash Sharma

Research paper thumbnail of Isolation and temporal endospermal expression of γ-kafirin gene of grain sorghum ( Sorghum bicolor L. moench) var. M 35-1 for introgression analysis of transgene

Journal of Cereal Science, 2008

A gamma-kafirin gene of Sorghum bicolor L var. M35-1 was isolated using a PCR based approach by d... more A gamma-kafirin gene of Sorghum bicolor L var. M35-1 was isolated using a PCR based approach by designing specific primers. The primers gave highly reproducible amplification. The amplicons were then cloned and sequenced. Nucleotide sequences were subjected to the homology search and a comparative analysis was done with other prolamins. Amplified γ-kafirin gene (accession no. AY566298 and AY566299) showed 99% homology with a γ-kafirin gene of Sorghum vulgare. Compared to Sorghum vulgare, only 3 extra bases were present in Sorghum bicolor at position 40 nucleotide bases downstream of transcription initiation site. These sequences were related with prolamins of other genera, i.e. coix and maize with 84% sequence homology. The deduced protein sequence of γ-kafirin gene of S. bicolor (accession no. AAS73290) gave significant similarity of 99%, 79% and 77% with gamma-kafirin protein of S. vulgare, γ-zein and γ-coixin proteins, respectively. All cysteine residues of γ-kafirin were highly conserved. Probable secondary structure of gamma-kafirin protein was predicted using the online PSIPRED server. Study of temporal expression of the γ-kafirin gene is needed for analysing the expression pattern of introgressed gene(s) driven by its promoter. Temporal expression of gamma-kafirin gene in endosperm studied by semi quantitative RT–PCR with specifically designed primers showed that γ-kafirin expression started at 7 DAP (days after pollination). There was statistically a non-significant increase in expression up to 14 DAP, thereafter expression increased significantly (at the 5% level) and reached a maximum at 21 DAP. Expression of γ-kafirin started decreasing after 21 DAP and a very low expression was detected at 28 DAP.

Research paper thumbnail of Optimizing melting rate and fuel consumption of rotary furnace using NSGA -II

… , 2008. CEC 2008.( …, 2008

Research paper thumbnail of Optimizing melting rate and fuel consumption of rotary furnace using NSGA -II

… , 2008. CEC 2008.( …, 2008

Research paper thumbnail of Design and biological evaluation of biphenyl-4-carboxylic acid hydrazide-hydrazone for antimicrobial activity

Synthesis, 2010

Seven biphenyl-4-carboxylic acid hydrazide-hydrazones have been synthesized. These hydrazone deri... more Seven biphenyl-4-carboxylic acid hydrazide-hydrazones have been synthesized. These hydrazone derivatives were characterized by CHN analysis, IR, and 1 H NMR spectral data. All the compounds were evaluated for their in vitro antimicrobial activity against two Gram negative strains (Escherichia coli and Pseudomonas aeruginosa) and two Gram positive strains (Bacillus subtilis and Staphylococcus aureus) and fungal strain Candida albicans and Aspergillus niger. All newly synthesized compounds exhibited promising results.

Research paper thumbnail of A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute

Euphytica, 2008

Jute is one of the most important fibre crops, which is second only to cotton in providing enviro... more Jute is one of the most important fibre crops, which is second only to cotton in providing environment-friendly (biodegradable and renewable) ligno-cellulose fibre. In order to improve this largely neglected crop, we conducted a preliminary study involving the following: (i) analysis of nature and extent of the genetic variability for fibre yield and four other related traits in a set of 81 genotypes belonging to two commercially cultivated Corchorus species (45 genotypes of C. olitorius + 36 genotypes of C. capsularis), (ii) development and analysis of a set of simple sequence repeat (SSR) markers from C. olitorius, and (iii) use of a sub-set of SSRs for assessment of genetic diversity in the above set of 81 genotypes. The results suggested quantitative nature of fibre yield and other related traits, with a preponderance of dominance component in genetic variance. A sub-set of 45 SSRs derived from C. olitorius, when used for a study of DNA polymorphism and genetic diversity, showed high transferability of these C. olitorius SSRs to C. capsularis. The average number of alleles for individual SSRs was surprisingly low (3.04 for both species, 2.02 for C. capsularis and 2.51 for C. olitorius), and so was the average polymorphic information content (PIC; 0.23 and 0.24 in two species). In the dendrogram obtained using a similarity matrix, the 81 genotypes were grouped into three clusters, which largely corresponded to the two species, Cluster I belonging mainly to C. capsularis and the other two closely related clusters (clusters II and III) belonging to C. olitorius. It was also shown that a minimum of 15 SSRs could give the same information as 41 SSRs, thus making many SSRs redundant. The SSR markers developed during the present study and to be developed in future will prove useful not only for evaluation of genetic diversity, but also for molecular mapping/QTL analysis, and for comparative genome analysis of the two Corchorus species.

Research paper thumbnail of The locus of origin of augmenting and reducing of visual evoked potentials in rat brain

Physiology & behavior, 1996

Humans who are high sensation seekers and cats who demonstrate comparable behavioral traits show ... more Humans who are high sensation seekers and cats who demonstrate comparable behavioral traits show increasing amplitudes of the early components of the cortical visual evoked potential (VEP) to increasing intensities of light flash; low sensation seekers show VEP reducing. Roman high-avoidance (RHA) and Roman low-avoidance (RLA) rats have behavioral traits comparable to human and cat high and low sensation seekers, respectively. Previously, we showed that RHA and RLA rats are cortical VEP augmenters and reducers, respectively. The goal of this study was to determine if augmenting-reducing is in fact a property of the visual cortex or if it originates at the lateral geniculate nucleus and is merely reflected in recordings from the cortex. VEPs to five flash intensities were recorded from the visual cortex and dorsal lateral geniculate of RHA and RLA rats. As in the previous study, the slope of the first cortical component as a function of flash intensity was greater in the RHA than in the RLA rats. The amplitude of the geniculate component that has a latency shorter than the first cortical component was no different in the two lines of rats. The finding from the cortex confirms the earlier finding of augmenting and reducing in RHA and RLA rats, respectively. The major new finding is that the augmenting-reducing difference recorded at the cortex does not occur at the thalamus, indicating that it is truly a cortical phenomenon.

Research paper thumbnail of Isolation and temporal endospermal expression of γ-kafirin gene of grain sorghum ( Sorghum bicolor L. moench) var. M 35-1 for introgression analysis of transgene

Journal of Cereal Science, 2008

A gamma-kafirin gene of Sorghum bicolor L var. M35-1 was isolated using a PCR based approach by d... more A gamma-kafirin gene of Sorghum bicolor L var. M35-1 was isolated using a PCR based approach by designing specific primers. The primers gave highly reproducible amplification. The amplicons were then cloned and sequenced. Nucleotide sequences were subjected to the homology search and a comparative analysis was done with other prolamins. Amplified γ-kafirin gene (accession no. AY566298 and AY566299) showed 99% homology with a γ-kafirin gene of Sorghum vulgare. Compared to Sorghum vulgare, only 3 extra bases were present in Sorghum bicolor at position 40 nucleotide bases downstream of transcription initiation site. These sequences were related with prolamins of other genera, i.e. coix and maize with 84% sequence homology. The deduced protein sequence of γ-kafirin gene of S. bicolor (accession no. AAS73290) gave significant similarity of 99%, 79% and 77% with gamma-kafirin protein of S. vulgare, γ-zein and γ-coixin proteins, respectively. All cysteine residues of γ-kafirin were highly conserved. Probable secondary structure of gamma-kafirin protein was predicted using the online PSIPRED server. Study of temporal expression of the γ-kafirin gene is needed for analysing the expression pattern of introgressed gene(s) driven by its promoter. Temporal expression of gamma-kafirin gene in endosperm studied by semi quantitative RT–PCR with specifically designed primers showed that γ-kafirin expression started at 7 DAP (days after pollination). There was statistically a non-significant increase in expression up to 14 DAP, thereafter expression increased significantly (at the 5% level) and reached a maximum at 21 DAP. Expression of γ-kafirin started decreasing after 21 DAP and a very low expression was detected at 28 DAP.

Research paper thumbnail of Optimizing melting rate and fuel consumption of rotary furnace using NSGA -II

… , 2008. CEC 2008.( …, 2008

Research paper thumbnail of Optimizing melting rate and fuel consumption of rotary furnace using NSGA -II

… , 2008. CEC 2008.( …, 2008

Research paper thumbnail of Design and biological evaluation of biphenyl-4-carboxylic acid hydrazide-hydrazone for antimicrobial activity

Synthesis, 2010

Seven biphenyl-4-carboxylic acid hydrazide-hydrazones have been synthesized. These hydrazone deri... more Seven biphenyl-4-carboxylic acid hydrazide-hydrazones have been synthesized. These hydrazone derivatives were characterized by CHN analysis, IR, and 1 H NMR spectral data. All the compounds were evaluated for their in vitro antimicrobial activity against two Gram negative strains (Escherichia coli and Pseudomonas aeruginosa) and two Gram positive strains (Bacillus subtilis and Staphylococcus aureus) and fungal strain Candida albicans and Aspergillus niger. All newly synthesized compounds exhibited promising results.

Research paper thumbnail of A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute

Euphytica, 2008

Jute is one of the most important fibre crops, which is second only to cotton in providing enviro... more Jute is one of the most important fibre crops, which is second only to cotton in providing environment-friendly (biodegradable and renewable) ligno-cellulose fibre. In order to improve this largely neglected crop, we conducted a preliminary study involving the following: (i) analysis of nature and extent of the genetic variability for fibre yield and four other related traits in a set of 81 genotypes belonging to two commercially cultivated Corchorus species (45 genotypes of C. olitorius + 36 genotypes of C. capsularis), (ii) development and analysis of a set of simple sequence repeat (SSR) markers from C. olitorius, and (iii) use of a sub-set of SSRs for assessment of genetic diversity in the above set of 81 genotypes. The results suggested quantitative nature of fibre yield and other related traits, with a preponderance of dominance component in genetic variance. A sub-set of 45 SSRs derived from C. olitorius, when used for a study of DNA polymorphism and genetic diversity, showed high transferability of these C. olitorius SSRs to C. capsularis. The average number of alleles for individual SSRs was surprisingly low (3.04 for both species, 2.02 for C. capsularis and 2.51 for C. olitorius), and so was the average polymorphic information content (PIC; 0.23 and 0.24 in two species). In the dendrogram obtained using a similarity matrix, the 81 genotypes were grouped into three clusters, which largely corresponded to the two species, Cluster I belonging mainly to C. capsularis and the other two closely related clusters (clusters II and III) belonging to C. olitorius. It was also shown that a minimum of 15 SSRs could give the same information as 41 SSRs, thus making many SSRs redundant. The SSR markers developed during the present study and to be developed in future will prove useful not only for evaluation of genetic diversity, but also for molecular mapping/QTL analysis, and for comparative genome analysis of the two Corchorus species.

Research paper thumbnail of The locus of origin of augmenting and reducing of visual evoked potentials in rat brain

Physiology & behavior, 1996

Humans who are high sensation seekers and cats who demonstrate comparable behavioral traits show ... more Humans who are high sensation seekers and cats who demonstrate comparable behavioral traits show increasing amplitudes of the early components of the cortical visual evoked potential (VEP) to increasing intensities of light flash; low sensation seekers show VEP reducing. Roman high-avoidance (RHA) and Roman low-avoidance (RLA) rats have behavioral traits comparable to human and cat high and low sensation seekers, respectively. Previously, we showed that RHA and RLA rats are cortical VEP augmenters and reducers, respectively. The goal of this study was to determine if augmenting-reducing is in fact a property of the visual cortex or if it originates at the lateral geniculate nucleus and is merely reflected in recordings from the cortex. VEPs to five flash intensities were recorded from the visual cortex and dorsal lateral geniculate of RHA and RLA rats. As in the previous study, the slope of the first cortical component as a function of flash intensity was greater in the RHA than in the RLA rats. The amplitude of the geniculate component that has a latency shorter than the first cortical component was no different in the two lines of rats. The finding from the cortex confirms the earlier finding of augmenting and reducing in RHA and RLA rats, respectively. The major new finding is that the augmenting-reducing difference recorded at the cortex does not occur at the thalamus, indicating that it is truly a cortical phenomenon.