Akihito Yamano - Profile on Academia.edu (original) (raw)

Hirokazu Kouguchi related author profile picture

Teiya Kijimoto related author profile picture

Pilar Garcia related author profile picture

Ken Inui related author profile picture

Bal R Singh related author profile picture

Ken Inui related author profile picture

Kazuaki Inoue related author profile picture

Abbas Hajizade related author profile picture

Martha Hale related author profile picture

F. Lebeda related author profile picture

Uploads

Papers by Akihito Yamano

Research paper thumbnail of Characterization of Toxin Complex Produced by a Unique Strain of Clostridium botulinum Serotype D 4947

The Protein Journal, 2000

A unique strain of Clostridium botulinum, serotype D 4947 (D-4947), produces a considerable amoun... more A unique strain of Clostridium botulinum, serotype D 4947 (D-4947), produces a considerable amount of a 650 kDa toxin complex (L-TC) and a small amount of a 280 kDa M-TC, a 540 kDa TC, and a 610 kDa TC. The complexes are composed of only un-nicked components, including neurotoxin (NT), nontoxic nonhemagglutinin (NTNHA) and hemagglutinin subcomponents (HA-70, HA-33 and HA-17). Unlike other NTs from all serotype strains, separation of D-4947 NT from L-TC, except for M-TC, during chromatography required highly alkaline conditions around pH 8.8. The separated NT and NTNHA/HAs complex can be reconstituted to L-TC that is indistinguishable from the parent L-TC with respect to toxicity, hemagglutination activity and gel filtration profile. The isoelectric points of NT and NTNHA/HAs were close together depending on the number of HA-33/17 molecules. We have established a new method to separate the unique D-4947 NT from the complex, which will yield valuable information on structure of botulinum toxin.

Research paper thumbnail of Characterization of Toxin Complex Produced by a Unique Strain of Clostridium botulinum Serotype D 4947

The Protein Journal, 2000

A unique strain of Clostridium botulinum, serotype D 4947 (D-4947), produces a considerable amoun... more A unique strain of Clostridium botulinum, serotype D 4947 (D-4947), produces a considerable amount of a 650 kDa toxin complex (L-TC) and a small amount of a 280 kDa M-TC, a 540 kDa TC, and a 610 kDa TC. The complexes are composed of only un-nicked components, including neurotoxin (NT), nontoxic nonhemagglutinin (NTNHA) and hemagglutinin subcomponents (HA-70, HA-33 and HA-17). Unlike other NTs from all serotype strains, separation of D-4947 NT from L-TC, except for M-TC, during chromatography required highly alkaline conditions around pH 8.8. The separated NT and NTNHA/HAs complex can be reconstituted to L-TC that is indistinguishable from the parent L-TC with respect to toxicity, hemagglutination activity and gel filtration profile. The isoelectric points of NT and NTNHA/HAs were close together depending on the number of HA-33/17 molecules. We have established a new method to separate the unique D-4947 NT from the complex, which will yield valuable information on structure of botulinum toxin.

Log In