Ala Habibian - Academia.edu (original) (raw)
Papers by Ala Habibian
European Journal of Pharmacology
PubMed, Aug 1, 2022
Exosomes are extracellular endosomal nanoparticles, which are formed under complex processes duri... more Exosomes are extracellular endosomal nanoparticles, which are formed under complex processes during the formation of multivesicular bodies. They are also achieved from conditioned media of a variety of cell types, especially mesenchymal stem cells (MSCs). Exosomes can modulate intracellular physiological actions via signaling molecules on the surface or secretion of components to the extracellular spaces. Furthermore, they are potentially used as crucial agents for cell-free therapy; however, their isolation and characterization can be challenging. In the current study, two methods of exosome isolation have been characterized and compared using a culture media of adipose-derived mesenchymal stem cells, namely ultracentrifugation and a commercial kit; moreover, the efficiency of these two methods was highlighted in this study. Two different isolation methods of exosomes from MSCs were used to compare the efficiency of exosomes. For both isolation methods, transmission electron microscopy, dynamic light scattering (DLS), and bicinchoninic acid (BCA) assay have been performed. The electron microscopy and DLS indicated the presence of exosomes. Moreover, the kit and ultracentrifugation isolates contained approximately comparable amounts of protein measured by the BCA. Overall, the two isolation methods had similar performances. Although ultracentrifugation is used as a gold standard for exosome isolation, the commercial kit has some advantages and can be applied alternatively according to its cost-effectiveness and time-saving properties.
Medical laboratory sciences, 2019
Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs)... more Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs) and their therapeutic application. Several factors such as being cost-benefit, efficiency, safety, yield and amount of produced cells are determinant for choosing the appropriate enzyme. Collagenase is a conventional enzyme commonly used for enzymatic digestion. However, other enzymes like trypsin and even combination of these enzymes can be used as an alternative strategy in different situations. Materials and Methods: Abdominal subcutaneous adipose tissue was obtained from male BALB/c mice and digested under three different enzymatic processes: collagenase and collagenase/trypsin and trypsin. Cell culture process was performed under standard condition and MSCs at 3rd passage were used for further characterization by flow cytometry. Results: In this study, two different enzymatic methods for digestion of adipose-derived MSCs (ADSCs) of BALB/c mice were investigated. The morphology of cells was pretty different and was more homogenous in collagenase group. Also the yield of cells was varied among groups. Furthermore, the obtained data from flow cytometry revealed that ADSCs were positive for CD90 (70%), CD29 (98%), CD105 (52%) and negative for CD45 (<2%).Conclusion: Application of different enzymes depends on various conditions. Altogether, these data indicate that although use of trypsin in isolation protocol is cost-benefit, it can be used as an alternative method whenever limited number of cells will be needed. However, collagenase as a well-known and conventional method can be used for isolation of larger quantity of cells with several applications.
Medical laboratory sciences, 2019
Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs)... more Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs) and their therapeutic application. Several factors such as being cost-benefit, efficiency, safety, yield and amount of produced cells are determinant for choosing the appropriate enzyme. Collagenase is a conventional enzyme commonly used for enzymatic digestion. However, other enzymes like trypsin and even combination of these enzymes can be used as an alternative strategy in different situations. Materials and Methods: Abdominal subcutaneous adipose tissue was obtained from male BALB/c mice and digested under three different enzymatic processes: collagenase and collagenase/trypsin and trypsin. Cell culture process was performed under standard condition and MSCs at 3rd passage were used for further characterization by flow cytometry. Results: In this study, two different enzymatic methods for digestion of adipose-derived MSCs (ADSCs) of BALB/c mice were investigated. The morphology of cells was pretty different and was more homogenous in collagenase group. Also the yield of cells was varied among groups. Furthermore, the obtained data from flow cytometry revealed that ADSCs were positive for CD90 (70%), CD29 (98%), CD105 (52%) and negative for CD45 (<2%).Conclusion: Application of different enzymes depends on various conditions. Altogether, these data indicate that although use of trypsin in isolation protocol is cost-benefit, it can be used as an alternative method whenever limited number of cells will be needed. However, collagenase as a well-known and conventional method can be used for isolation of larger quantity of cells with several applications.
Additional file of MSCs loaded with oncolytic reovirus: migration and in vivo virus delivery pote... more Additional file of MSCs loaded with oncolytic reovirus: migration and in vivo virus delivery potential for evaluating anti-cancer effect in tumor-bearing C57BL/6 mice
BACKGROUND: Bladder cancer is a common cancer worldwide whose incidence continues to increase. It... more BACKGROUND: Bladder cancer is a common cancer worldwide whose incidence continues to increase. It is estimated that there are 261,000 cases of bladder cancer resulting in 115,000 deaths worldwide. MATERIALS AND METHODS: A total of 40 Wistar rats were equalized into four groups: blank control, HHcy, folacin intervention, and lutein intervention groups. HHcy rat models were established. The intervention groups were respectively lavaged with folacin and lutein. Oxidative stress states, the levels of nitric oxide (NO) and endothelin-1 (ET-1), as well as the expression of nuclear factor (NF)-κ κB p65 and intercellular adhesion molecule (ICAM)-1 were compared. CONCLUSIONS: Oxidative stress and inflammation are the important mechanisms of HHcymediated AS. In particular, HHcy-induced aggravated oxidative stress may function as the initial AS-mediating mechanism, upregulating the expression of NF-κ κB p65 and ICAM-1 and thereby becoming associated with AS. Lutein noticeably intervenes in and inhibits Hcy-mediated oxidative stress excitement and downregulates the expression of inflammation-associated informational molecules.
Journal of Immunoassay and Immunochemistry
Avicenna Journal of Clinical Microbiology and Infection
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease ... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19) as a pandemic infectious disease which has led to thousands of deaths around the world. The Coronaviridae family is the second cause of the common cold that targets human respiratory tracts. Specific diagnostic laboratory tests in addition to clinical investigations would be helpful in confirming COVID-19 in the early stages for controlling the disease. Upon SARS-CoV-2 infection, antibody responses are produced during the early phase of illness (>7 days), meanwhile, viral nucleic acid real-time reverse-transcription polymerase chain reaction (rRT-PCR) test is applied as the confirmatory assay in the first 5-6 days after the onset of illness. Due to the rise of antibodies, the viral nucleic acid represents a gradual decline. These laboratory tests may be considered valuable for monitoring the patient’s status to prevent the spreading of infections and keep him/her in qu...
archives of razi institute, 2021
Colorectal cancer is the fourth leading cause-related death of cancer which has significantly inc... more Colorectal cancer is the fourth leading cause-related death of cancer which has significantly increased over the past three decades. New therapeutic approaches such as oncolytic viruses become very imperative recently to destruction of cancer cells. Use of mesenchymal stem cells secretome which are produced in response to variant conditions involves different paracrine molecules secretion that they have therapeutic potential in several chronic diseases. Mesenchymal stem cells and their derivative are used as a regenerative medicine, but there is ambiguity in the function of these cells in control of malignancy. In this study, we aim to examine the apoptotic effect of secretome derived from mesenchymal stem cells affected by encompassing oncolytic reoviruses. Mesenchymal stem cells were cultured after separation from abdominal adipose tissue of BALB/c mice. After three passages, the cells infected by reovirus at Multiplicity of Infection (MOI) of 1 Plaque-Forming Unit (PFU) per cell....
Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infec... more Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive...
Background: Epstein-Barr Virus (EBV) is a DNA virus which belongs to the Herpesviridae family and... more Background: Epstein-Barr Virus (EBV) is a DNA virus which belongs to the Herpesviridae family and in γ-herpesvirus subfamily, has been infected about 95% of the world's population. EBV is transmitted through saliva and associated with different disease such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitts lymphoma, Hodgkin and Non-Hodgkins lymphoma. The study proposed to determine the frequency of Epstein-Barr Virus expression in histological tissue of Non-Hodgkin's lymphoma in Ahvaz, Iran. Materials and methods: In this study 29 samples of Non- Hodgkins lymphoma were examined from Ahvaz Imam Khomeini Hospital, using Nested-PCR technique on EBNA-1 region in order to determine the presence of EBV genome in tumoral tissues. Results: Among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. Out of 14 samples of Non- Hodgkins lymphoma, 4(28.57%) were female and 10 (71.42%) were male. A number of 8 (57%) cases belonged to the adults age gr...
Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, w... more Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, which are prevalent worldwide. Chronic HBV may lead to cirrhosis and hepatocellular carcinoma. Several epidemiological studies have indicated that hepatitis B virus is involved in B-cell Hodgkin and Non-Hodgkin Lymphoma (NHL). Objectives: The aim of this study was to evaluate the association between hepatitis B infection and Hodgkin and non-Hodgkin Lymphoma. Materials and Methods: Paraffin embedded of 41 block samples including 12 (29.26%) Hodgkin and 29 (70.73%) non-Hodgkin patients were collected. Next, DNA extraction was carried out for all the samples followed by HBV DNA detection by the nested polymerase chain reaction (PCR). The positive HBV DNA samples were sequenced, and HBV genotypes and HBV subtypes were determined. Results: Three out of 12 (25%) Hodgkin samples and seven out of 29 (24.13%) non-Hodgkin showed positive HBV DNA results. The results of sequencing revealed that the ...
Cancer Cell International
Background and aims Several oncolytic viruses applications have been approved in the clinic or in... more Background and aims Several oncolytic viruses applications have been approved in the clinic or in different phases of clinical trials. However, these methods have some rudimentary problems. Therefore, to enhance the delivery and quality of treatment, considering the advantage of cell carrier-based methods such as Mesenchymal Stem Cells (MSC) have been proposed. This study was designed to evaluate the performance and quality of cancer treatment based on MSCs loaded by oncolytic reovirus in the cancerous C57BL/6 mouse model. Also, we evaluated MSCs migration potency in vitro and in vivo following the oncolytic reovirus infection. Methods C57BL/6 mice were inoculated with TC-1 cell lines and tumors were established in the right flank. Mice were systemically treated with reovirus, MSCs-loaded with reovirus, MSCs, and PBS as a control in separated groups. Effects of infected AD-MSCs with reovirus on tumor growth and penetration in the tumor site were monitored. All groups of mice were mo...
Jundishapur Journal of Microbiology, 2015
Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, w... more Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, which are prevalent worldwide. Chronic HBV may lead to cirrhosis and hepatocellular carcinoma. Several epidemiological studies have indicated that hepatitis B virus is involved in B-cell Hodgkin and Non-Hodgkin Lymphoma (NHL). Objectives: The aim of this study was to evaluate the association between hepatitis B infection and Hodgkin and non-Hodgkin Lymphoma. Materials and Methods: Paraffin embedded of 41 block samples including 12 (29.26%) Hodgkin and 29 (70.73%) non-Hodgkin patients were collected. Next, DNA extraction was carried out for all the samples followed by HBV DNA detection by the nested polymerase chain reaction (PCR). The positive HBV DNA samples were sequenced, and HBV genotypes and HBV subtypes were determined. Results: Three out of 12 (25%) Hodgkin samples and seven out of 29 (24.13%) non-Hodgkin showed positive HBV DNA results. The results of sequencing revealed that the D genotype was predominant among the positive HBV patients. Interestingly an unpredictable amino acid proline was detected in position 88 of the HBs gene, which indicates a new mutation in the "S" region of HBV DNA in patients with Hodgkin and non-Hodgkin lymphoma. Conclusions: A high rate of 25% and 24.13% of HBV DNA was detected among patients with Hodgkin and non-Hodgkin lymphoma, respectively.
European Journal of Pharmacology
PubMed, Aug 1, 2022
Exosomes are extracellular endosomal nanoparticles, which are formed under complex processes duri... more Exosomes are extracellular endosomal nanoparticles, which are formed under complex processes during the formation of multivesicular bodies. They are also achieved from conditioned media of a variety of cell types, especially mesenchymal stem cells (MSCs). Exosomes can modulate intracellular physiological actions via signaling molecules on the surface or secretion of components to the extracellular spaces. Furthermore, they are potentially used as crucial agents for cell-free therapy; however, their isolation and characterization can be challenging. In the current study, two methods of exosome isolation have been characterized and compared using a culture media of adipose-derived mesenchymal stem cells, namely ultracentrifugation and a commercial kit; moreover, the efficiency of these two methods was highlighted in this study. Two different isolation methods of exosomes from MSCs were used to compare the efficiency of exosomes. For both isolation methods, transmission electron microscopy, dynamic light scattering (DLS), and bicinchoninic acid (BCA) assay have been performed. The electron microscopy and DLS indicated the presence of exosomes. Moreover, the kit and ultracentrifugation isolates contained approximately comparable amounts of protein measured by the BCA. Overall, the two isolation methods had similar performances. Although ultracentrifugation is used as a gold standard for exosome isolation, the commercial kit has some advantages and can be applied alternatively according to its cost-effectiveness and time-saving properties.
Medical laboratory sciences, 2019
Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs)... more Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs) and their therapeutic application. Several factors such as being cost-benefit, efficiency, safety, yield and amount of produced cells are determinant for choosing the appropriate enzyme. Collagenase is a conventional enzyme commonly used for enzymatic digestion. However, other enzymes like trypsin and even combination of these enzymes can be used as an alternative strategy in different situations. Materials and Methods: Abdominal subcutaneous adipose tissue was obtained from male BALB/c mice and digested under three different enzymatic processes: collagenase and collagenase/trypsin and trypsin. Cell culture process was performed under standard condition and MSCs at 3rd passage were used for further characterization by flow cytometry. Results: In this study, two different enzymatic methods for digestion of adipose-derived MSCs (ADSCs) of BALB/c mice were investigated. The morphology of cells was pretty different and was more homogenous in collagenase group. Also the yield of cells was varied among groups. Furthermore, the obtained data from flow cytometry revealed that ADSCs were positive for CD90 (70%), CD29 (98%), CD105 (52%) and negative for CD45 (<2%).Conclusion: Application of different enzymes depends on various conditions. Altogether, these data indicate that although use of trypsin in isolation protocol is cost-benefit, it can be used as an alternative method whenever limited number of cells will be needed. However, collagenase as a well-known and conventional method can be used for isolation of larger quantity of cells with several applications.
Medical laboratory sciences, 2019
Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs)... more Background: Enzymatic digestion is an essential stage for culturing Mesenchymal Stem Cells (MSCs) and their therapeutic application. Several factors such as being cost-benefit, efficiency, safety, yield and amount of produced cells are determinant for choosing the appropriate enzyme. Collagenase is a conventional enzyme commonly used for enzymatic digestion. However, other enzymes like trypsin and even combination of these enzymes can be used as an alternative strategy in different situations. Materials and Methods: Abdominal subcutaneous adipose tissue was obtained from male BALB/c mice and digested under three different enzymatic processes: collagenase and collagenase/trypsin and trypsin. Cell culture process was performed under standard condition and MSCs at 3rd passage were used for further characterization by flow cytometry. Results: In this study, two different enzymatic methods for digestion of adipose-derived MSCs (ADSCs) of BALB/c mice were investigated. The morphology of cells was pretty different and was more homogenous in collagenase group. Also the yield of cells was varied among groups. Furthermore, the obtained data from flow cytometry revealed that ADSCs were positive for CD90 (70%), CD29 (98%), CD105 (52%) and negative for CD45 (<2%).Conclusion: Application of different enzymes depends on various conditions. Altogether, these data indicate that although use of trypsin in isolation protocol is cost-benefit, it can be used as an alternative method whenever limited number of cells will be needed. However, collagenase as a well-known and conventional method can be used for isolation of larger quantity of cells with several applications.
Additional file of MSCs loaded with oncolytic reovirus: migration and in vivo virus delivery pote... more Additional file of MSCs loaded with oncolytic reovirus: migration and in vivo virus delivery potential for evaluating anti-cancer effect in tumor-bearing C57BL/6 mice
BACKGROUND: Bladder cancer is a common cancer worldwide whose incidence continues to increase. It... more BACKGROUND: Bladder cancer is a common cancer worldwide whose incidence continues to increase. It is estimated that there are 261,000 cases of bladder cancer resulting in 115,000 deaths worldwide. MATERIALS AND METHODS: A total of 40 Wistar rats were equalized into four groups: blank control, HHcy, folacin intervention, and lutein intervention groups. HHcy rat models were established. The intervention groups were respectively lavaged with folacin and lutein. Oxidative stress states, the levels of nitric oxide (NO) and endothelin-1 (ET-1), as well as the expression of nuclear factor (NF)-κ κB p65 and intercellular adhesion molecule (ICAM)-1 were compared. CONCLUSIONS: Oxidative stress and inflammation are the important mechanisms of HHcymediated AS. In particular, HHcy-induced aggravated oxidative stress may function as the initial AS-mediating mechanism, upregulating the expression of NF-κ κB p65 and ICAM-1 and thereby becoming associated with AS. Lutein noticeably intervenes in and inhibits Hcy-mediated oxidative stress excitement and downregulates the expression of inflammation-associated informational molecules.
Journal of Immunoassay and Immunochemistry
Avicenna Journal of Clinical Microbiology and Infection
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease ... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19) as a pandemic infectious disease which has led to thousands of deaths around the world. The Coronaviridae family is the second cause of the common cold that targets human respiratory tracts. Specific diagnostic laboratory tests in addition to clinical investigations would be helpful in confirming COVID-19 in the early stages for controlling the disease. Upon SARS-CoV-2 infection, antibody responses are produced during the early phase of illness (>7 days), meanwhile, viral nucleic acid real-time reverse-transcription polymerase chain reaction (rRT-PCR) test is applied as the confirmatory assay in the first 5-6 days after the onset of illness. Due to the rise of antibodies, the viral nucleic acid represents a gradual decline. These laboratory tests may be considered valuable for monitoring the patient’s status to prevent the spreading of infections and keep him/her in qu...
archives of razi institute, 2021
Colorectal cancer is the fourth leading cause-related death of cancer which has significantly inc... more Colorectal cancer is the fourth leading cause-related death of cancer which has significantly increased over the past three decades. New therapeutic approaches such as oncolytic viruses become very imperative recently to destruction of cancer cells. Use of mesenchymal stem cells secretome which are produced in response to variant conditions involves different paracrine molecules secretion that they have therapeutic potential in several chronic diseases. Mesenchymal stem cells and their derivative are used as a regenerative medicine, but there is ambiguity in the function of these cells in control of malignancy. In this study, we aim to examine the apoptotic effect of secretome derived from mesenchymal stem cells affected by encompassing oncolytic reoviruses. Mesenchymal stem cells were cultured after separation from abdominal adipose tissue of BALB/c mice. After three passages, the cells infected by reovirus at Multiplicity of Infection (MOI) of 1 Plaque-Forming Unit (PFU) per cell....
Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infec... more Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive...
Background: Epstein-Barr Virus (EBV) is a DNA virus which belongs to the Herpesviridae family and... more Background: Epstein-Barr Virus (EBV) is a DNA virus which belongs to the Herpesviridae family and in γ-herpesvirus subfamily, has been infected about 95% of the world's population. EBV is transmitted through saliva and associated with different disease such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitts lymphoma, Hodgkin and Non-Hodgkins lymphoma. The study proposed to determine the frequency of Epstein-Barr Virus expression in histological tissue of Non-Hodgkin's lymphoma in Ahvaz, Iran. Materials and methods: In this study 29 samples of Non- Hodgkins lymphoma were examined from Ahvaz Imam Khomeini Hospital, using Nested-PCR technique on EBNA-1 region in order to determine the presence of EBV genome in tumoral tissues. Results: Among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. Out of 14 samples of Non- Hodgkins lymphoma, 4(28.57%) were female and 10 (71.42%) were male. A number of 8 (57%) cases belonged to the adults age gr...
Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, w... more Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, which are prevalent worldwide. Chronic HBV may lead to cirrhosis and hepatocellular carcinoma. Several epidemiological studies have indicated that hepatitis B virus is involved in B-cell Hodgkin and Non-Hodgkin Lymphoma (NHL). Objectives: The aim of this study was to evaluate the association between hepatitis B infection and Hodgkin and non-Hodgkin Lymphoma. Materials and Methods: Paraffin embedded of 41 block samples including 12 (29.26%) Hodgkin and 29 (70.73%) non-Hodgkin patients were collected. Next, DNA extraction was carried out for all the samples followed by HBV DNA detection by the nested polymerase chain reaction (PCR). The positive HBV DNA samples were sequenced, and HBV genotypes and HBV subtypes were determined. Results: Three out of 12 (25%) Hodgkin samples and seven out of 29 (24.13%) non-Hodgkin showed positive HBV DNA results. The results of sequencing revealed that the ...
Cancer Cell International
Background and aims Several oncolytic viruses applications have been approved in the clinic or in... more Background and aims Several oncolytic viruses applications have been approved in the clinic or in different phases of clinical trials. However, these methods have some rudimentary problems. Therefore, to enhance the delivery and quality of treatment, considering the advantage of cell carrier-based methods such as Mesenchymal Stem Cells (MSC) have been proposed. This study was designed to evaluate the performance and quality of cancer treatment based on MSCs loaded by oncolytic reovirus in the cancerous C57BL/6 mouse model. Also, we evaluated MSCs migration potency in vitro and in vivo following the oncolytic reovirus infection. Methods C57BL/6 mice were inoculated with TC-1 cell lines and tumors were established in the right flank. Mice were systemically treated with reovirus, MSCs-loaded with reovirus, MSCs, and PBS as a control in separated groups. Effects of infected AD-MSCs with reovirus on tumor growth and penetration in the tumor site were monitored. All groups of mice were mo...
Jundishapur Journal of Microbiology, 2015
Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, w... more Background: Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, which are prevalent worldwide. Chronic HBV may lead to cirrhosis and hepatocellular carcinoma. Several epidemiological studies have indicated that hepatitis B virus is involved in B-cell Hodgkin and Non-Hodgkin Lymphoma (NHL). Objectives: The aim of this study was to evaluate the association between hepatitis B infection and Hodgkin and non-Hodgkin Lymphoma. Materials and Methods: Paraffin embedded of 41 block samples including 12 (29.26%) Hodgkin and 29 (70.73%) non-Hodgkin patients were collected. Next, DNA extraction was carried out for all the samples followed by HBV DNA detection by the nested polymerase chain reaction (PCR). The positive HBV DNA samples were sequenced, and HBV genotypes and HBV subtypes were determined. Results: Three out of 12 (25%) Hodgkin samples and seven out of 29 (24.13%) non-Hodgkin showed positive HBV DNA results. The results of sequencing revealed that the D genotype was predominant among the positive HBV patients. Interestingly an unpredictable amino acid proline was detected in position 88 of the HBs gene, which indicates a new mutation in the "S" region of HBV DNA in patients with Hodgkin and non-Hodgkin lymphoma. Conclusions: A high rate of 25% and 24.13% of HBV DNA was detected among patients with Hodgkin and non-Hodgkin lymphoma, respectively.