Albená Nunes Silva - Academia.edu (original) (raw)

Papers by Albená Nunes Silva

Gazzetta Medica Italiana Archivio per le Scienze Mediche

Frontiers in Physiology

Introduction: Physical exercise can acutely and chronically modulate immunological responses. Wom... more Introduction: Physical exercise can acutely and chronically modulate immunological responses. Women and men have different innate and adaptive immune responses, and in this sense, these two groups may also have different acute immunological responses induced by exercise. In addition, it is essential to understand further whether the effects of physical exercise on the immune system responses depend on sex because limited scientific evidence on this topic is available. This information may allow athletes and coaches to improve the training process, mainly to understand if the physiological impact of given training stimuli in women is similar to that in men.Objective: The present study aimed to investigate the acute effects of continuous submaximal exercise until fatigue on physiological and immunological parameters in amateur female and male runners.Methods: This study included 18 female and 15 male volunteers. Each participant visited the laboratory on four consecutive days. The fir...

Frontiers in endocrinology, 2017

Organotins (OTs) are organometallic pollutants. The OTs are organometallic pollutants that are us... more Organotins (OTs) are organometallic pollutants. The OTs are organometallic pollutants that are used in many industrial, agricultural, and domestic products, and it works as powerful biocidal compound against large types of microorganisms such as fungi and bacteria. In addition, OTs are well known to be endocrine-disrupting chemicals, leading abnormalities an "imposex" phenomenon in the female mollusks. There are some studies showing that OTs' exposure is responsible for neural, endocrine, and reproductive dysfunctionsandmodels. However, OTs' effects over the mammalian immune system are poorly understood, particularly in respiratory diseases. The immune system, as well as their cellular components, performs a pivotal role in the control of the several physiologic functions, and in the maintenance and recovery of homeostasis. Thus, it is becoming important to better understand the association between environmental contaminants, as OTs, and the physiological function ...

Journal of Sports Medicine & Doping Studies, 2013

Intense exercise is a physiological stress capable of inducing damage in the exercised muscle gro... more Intense exercise is a physiological stress capable of inducing damage in the exercised muscle group. As a consequence of this exercise-induced micro lesion, there is an increase in the interaction of leukocytes (neutrophils and monocytes/macrophages) with muscle endothelial cells and their transmigration into skeletal muscle tissue. Mechanisms driving this physiological response are not known, but exercise-induced muscle damage may be associated with a local inflammation involving leukocyte accumulation in damaged muscle tissue. Important recent findings show that this infiltration of leukocytes plays an important role in the skeletal muscle remodeling process by activating angiogenesis and hypertrophic pathways inside the skeletal muscle tissue. There are some speculations suggesting that this inflammatory process should be controlled by anti-inflammation drugs in athletes to accelerate the recovery time. Investigations of the literature in this field up to this point do not offer a sufficient body of evidence confirming the need for an anti-inflammatory drug administration to control the exercise-induced inflammation. On the other hand, anti-inflammatory drug administration as a supplemental diet could inhibit important pathways of tissue adaptative responses and block the benefits of exercise to the skeletal muscle tissue.

Protein and peptide letters, Jul 28, 2017

Many physiological responses of the Renin Angiotensin System (RAS) are associated with two opposi... more Many physiological responses of the Renin Angiotensin System (RAS) are associated with two opposite pathways: (1) a classical one formed by angiotensin-converting enzyme (ACE), Angiotensin II (Ang II) and Angiotensin type 1 (AT1) receptor, which is associated to vasoconstriction, cell proliferation, organ hypertrophy, sodium retention and aldosterone release and (2) a counter-regulatory or vasodilator pathway comprising angiotensin-converting enzyme 2 (ACE2), Angiotensin-(1-7) [Ang-(1-7)] and Mas receptor, which is involved in vasodilation, anti-proliferation, anti-hypertrophy, cardioprotective and renoprotective actions. This review aimed to bring up-to-date on the interactions between physical exercise and the vasodilator axis of the RAS (ACE2-Ang-(1-7)-Mas receptor axis). Herein, we reported studies in animal models and in humans and we also investigated the relation of acute and chronic exercise with blood pressure regulation and components of the vasodilator axis of the RAS. We...

Oxidative medicine and cellular longevity, 2012

This review offers an overview of the influence of reactive species produced during exercise and ... more This review offers an overview of the influence of reactive species produced during exercise and their effect on exercise adaptation. Reactive species and free radicals are unstable molecules that oxidize other molecules in order to become stable. Although they play important roles in our body, they can also lead to oxidative stress impairing diverse cellular functions. During exercise, reactive species can be produced mainly, but not exclusively, by the following mechanisms: electron leak at the mitochondrial electron transport chain, ischemia/reperfusion and activation of endothelial xanthine oxidase, inflammatory response, and autooxidation of catecholamines. Chronic exercise also leads to the upregulation of the body's antioxidant defence mechanism, which helps minimize the oxidative stress that may occur after an acute bout of exercise. Recent studies show a beneficial role of the reactive species, produced during a bout of exercise, that lead to important training adaptati...

Inflammation Research, 2013

Objective Interleukin-4 (IL-4) is a multifunctional cytokine involved in many diseases such as au... more Objective Interleukin-4 (IL-4) is a multifunctional cytokine involved in many diseases such as autoimmune hepatitis and idiosyncratic drug reactions. However, its role in acetaminophen (APAP)-induced liver injury remains unclear. Our objective was to evaluate the contribution of IL-4 to the pathogenesis of APAP-induced liver injury. Methods Balb/C (WT) and IL-4 knockout (IL-4-/-) mice were orally overdosed with APAP. After 24 h, survival percentage, biochemical and morphological markers of liver injury, and tissue inflammation were assessed. Results IL-4-/mice were protected from APAP toxicity. Intravital confocal microscopy, tissue histology and serum ALT levels showed significantly less liver injury and inflammation than in the WT group, which may explain the increased survival rate of IL-4-/mice. In addition, IL-4-/mice had decreased production of tumor necrosis factor a, CXCL1 and interleukin-1b in the liver, but not in a remote site such as the lungs. Hepatic macrophage activation was markedly reduced in IL-4-deficient mice. In addition, glutathione depletion-a primary cause of APAPmediated injury-was significantly attenuated in IL-4-/mice. Conclusions Taken together, our data demonstrate that IL-4-/mice are protected from APAP-induced liver injury due to reduced depletion of glutathione, which prevented liver damage and tissue inflammation.

PLoS ONE, 2014

Intense exercise is a physiological stress capable of inducing the interaction of neutrophils wit... more Intense exercise is a physiological stress capable of inducing the interaction of neutrophils with muscle endothelial cells and their transmigration into tissue. Mechanisms driving this physiological inflammatory response are not known. Here, we investigate whether production of reactive oxygen species is relevant for neutrophil interaction with endothelial cells and recruitment into the quadriceps muscle in mice subjected to the treadmill fatiguing exercise protocol. Mice exercised until fatigue by running for 56.366.8 min on an electric treadmill. Skeletal muscle was evaluated by intravital microscopy at different time points after exercise, and then removed to assess local oxidative stress and histopathological analysis. We observed an increase in plasma lactate and creatine kinase (CK) concentrations after exercise. The numbers of monocytes, neutrophils, and lymphocytes in blood increased 12 and 24 hours after the exercise. Numbers of rolling and adherent leukocytes increased 3, 6, 12, and 24 hours post-exercise, as assessed by intravital microscopy. Using LysM-eGFP mice and confocal intravital microscopy technology, we show that the number of transmigrating neutrophils increased 12 hours post-exercise. Mutant gp91 phox-/-(non-functional NADPH oxidase) mice and mice treated with apocynin showed diminished neutrophil recruitment. SOD treatment promoted further adhesion and transmigration of leukocytes 12 hours after the exercise. These findings confirm our hypothesis that treadmill exercise increases the recruitment of leukocytes to the postcapillary venules, and NADPH oxidase-induced ROS plays an important role in this process.

Biology

Background: to evaluate the effects of one week of supplementation with curcumin combined with pi... more Background: to evaluate the effects of one week of supplementation with curcumin combined with piperine on physical performance, immune system cell counts, muscle damage, and plasma levels of inflammatory markers after a treadmill running training session. Methods: This study is a double-blind, crossover-balanced clinical trial with a three-week intervention. Sixteen male runners with a mean age of 36 ± 9 years and VO2 max of 60.6 ± 9.03 mL.kg −1 min −1 were recruited and randomly divided into 2 groups: the first group (CPG) was supplemented daily for 7 days with 500 mg of curcumin + 20 mg piperine, and the second group (PG) was supplemented with 540 mg of cellulose. After the 7th day of supplementation, the volunteers participated in the experimental running protocol, where blood samples were collected before, after, and one hour after exercise for analysis of the number of leukocytes, creatine kinase, and cytokine concentration (IL-2, TNF-α, IFN, IL-6, and IL-10) using flow cytome...

Sport Sciences for Health, 2021

Purpose We investigated lower limb strength training (LLST)’s effect on the levels of renin angio... more Purpose We investigated lower limb strength training (LLST)’s effect on the levels of renin angiotensin system (RAS) molecules in urine and serum. Methods Twelve male volunteers with 23.4 ± 2.7 years old performed our LLST protocol for 10 weeks. The effect of LLST was evaluated based on urine and serum levels of RAS molecules, including Angiotensin II, Ang-(1–7), angiotensin converting enzyme ACE 1 and ACE 2, before and immediately after the first (S1) and the last (S30) training sessions. Results The training protocol significantly reduced the percentage of fat and increased the total lean mass. Serum and urinary levels of Ang II were higher after the last session in comparison to levels before this session as well as if compared to values after the first exercise session. Levels of Ang-(1–7) significantly increased in serum and urine after first and last sessions and in the comparison to values before and after last session to those before and after first session, respectively. Levels of ACE1 and ACE2 did not modify in any condition. In addition, LLST seems to chronically shift the balance between both RAS axes toward the alternative axis based on the analysis of Ang-(1–7)/Ang II ratio. Conclusion LLST acutely and chronically modified serum and urinary concentrations of Ang II and Ang-(1–7).

Medicina, 2020

Background and objective: Post-exercise hypotension, the reduction of blood pressure after a bout... more Background and objective: Post-exercise hypotension, the reduction of blood pressure after a bout of exercise, is of great clinical relevance. Resistance exercise training is considered an important contribution to exercise training programs for hypertensive individuals and athletes. In this context, post-exercise hypotension could be clinically relevant because it would maintain blood pressure of hypertensive individuals transiently at lower levels during day-time intervals, when blood pressure is typically at its highest levels. The aim of this study was to compare the post-exercise cardiovascular effects on Paralympic powerlifting athletes of two typical high-intensity resistance-training sessions, using either five sets of five bench press repetitions at 90% 1 repetition maximum (1RM) or five sets of three bench press repetitions at 95% 1RM. Materials and Methods: Ten national-level Paralympic weightlifting athletes (age: 26.1 ± 6.9 years; body mass: 76.8 ± 17.4 kg) completed th...

International Journal of Environmental Research and Public Health, 2019

Obesity is a highly prevalent chronic metabolic disease, with an increasing incidence, and is cur... more Obesity is a highly prevalent chronic metabolic disease, with an increasing incidence, and is currently approaching epidemic proportions in developing countries. Ouraim was to evaluate the activity levels, quality of life (QoL), clinical parameters, laboratory parameters, and cardiometabolic risk factors afterbariatric surgery (BS). We classified78 patients who underwentBS into four groups, as follows: Those evaluated 1–2 years after BS (BS2), 2–4 years after BS (BS4), 4–6 years after BS (BS6), and 6–10 years after BS (BS+6). Body weight (BW), body mass index (BMI), comorbidities associated with obesity (ACRO), physical activity level, and QoL were evaluated. Patients exhibited improvements in BW, BMI, cardiometabolic risk, hypertension, dyslipidemia, and diabetes and significant changes in lipid profiles in the first postoperative yearafter BS.The physical activity level inthe BS2, BS4, and BS6 groups was increased, compared with that in the first postoperative year, with a decreas...

Annals of Research in Sport and Physical Activity, 2018

A navegação consulta e descarregamento dos títulos inseridos nas Bibliotecas Digitais UC Digitali... more A navegação consulta e descarregamento dos títulos inseridos nas Bibliotecas Digitais UC Digitalis, UC Pombalina e UC Impactum, pressupõem a aceitação plena e sem reservas dos Termos e Condições de Uso destas Bibliotecas Digitais, disponíveis em https://digitalis.uc.pt/pt-pt/termos. Conforme exposto nos referidos Termos e Condições de Uso, o descarregamento de títulos de acesso restrito requer uma licença válida de autorização devendo o utilizador aceder ao(s) documento(s) a partir de um endereço de IP da instituição detentora da supramencionada licença. Ao utilizador é apenas permitido o descarregamento para uso pessoal, pelo que o emprego do(s) título(s) descarregado(s) para outro fim, designadamente comercial, carece de autorização do respetivo autor ou editor da obra. Na medida em que todas as obras da UC Digitalis se encontram protegidas pelo Código do Direito de Autor e Direitos Conexos e demais legislação aplicável, toda a cópia, parcial ou total, deste documento, nos casos em que é legalmente admitida, deverá conter ou fazer-se acompanhar por este aviso.

African Journal of Microbiology Research, 2012

Physical exercise is a known factor that can promote oxidative stress and may result in cellular ... more Physical exercise is a known factor that can promote oxidative stress and may result in cellular damage if not neutralized by antioxidant mechanisms. This study aimed to determine if the level of hepatic oxidative stress resulting from high-intensity interval training (HIIT) is affected by the frequency pattern (consecutive vs. non-consecutive) of the training sessions. Thirty-two Wistar rats were divided equally into four groups: two control groups, (CS1) and (CS2), remained sedentary throughout the experiment, and two test groups, (CT1) and (CT2), and were submitted to HIIT for 12 consecutive and non-consecutive (12 sessions, 3 times/wk over four weeks) days, respectively. There were no significant differences in markers of oxidative damage measured in hepatic tissue (TBARS) and markers of antioxidant activity (Sulfhydryl, FRAP), as well as markers of hepatic damage (AST and ALT) and antioxidant defense (Uric Acid) measured in plasma of both HIIT groups after training compared to ...

Frontiers in Cell and Developmental Biology, 2021

Acute exercise increases the amount of circulating inflammatory cells and cytokines to maintain p... more Acute exercise increases the amount of circulating inflammatory cells and cytokines to maintain physiological homeostasis. However, it remains unclear how physical training regulates exercise-induced inflammation and performance. Here, we demonstrate that acute high intensity exercise promotes an inflammatory profile characterized by increased blood IL-6 levels, neutrophil migratory capacity, and leukocyte recruitment to skeletal muscle vessels. Moreover, we found that physical training amplified leukocyte–endothelial cell interaction induced by acute exercise in skeletal muscle vessels and diminished exercise-induced inflammation in skeletal muscle tissue. Furthermore, we verified that disruption of the gp-91 subunit of NADPH-oxidase inhibited exercise-induced leukocyte recruitment on skeletal muscle after training with enhanced exercise time until fatigue. In conclusion, the training was related to physical improvement and immune adaptations. Moreover, reactive oxygen species (ROS...

Na medida em que todas as obras da UC Digitalis se encontram protegidas pelo Código do Direito de... more Na medida em que todas as obras da UC Digitalis se encontram protegidas pelo Código do Direito de Autor e Direitos Conexos e demais legislação aplicável, toda a cópia, parcial ou total, deste documento, nos casos em que é legalmente admitida, deverá conter ou fazer-se acompanhar por este aviso. The role of inflammation in physical training induced skeletal muscle remodeling: the reactive oxygen species (ROS) participation

Movimento (Porto Alegre)

Monitorar e analisar a execução das metas pactuadas no Planejamento Estratégico da Escola de Educ... more Monitorar e analisar a execução das metas pactuadas no Planejamento Estratégico da Escola de Educação Física da Universidade Federal de Ouro Preto, nos 18 primeiros meses de implementação. Realizaram-se 15 entrevistas semiestruturadas com 12 mentores e três discentes que ocupam representações estudantis. Utilizou-se análise de conteúdo, possibilitando delineamento de quatro categorias: a) resultados preliminares obtidos; b) potencialidades do instrumento; c) barreiras e enfrentamentos; d) propostas de aperfeiçoamento. Observou-se que a maioria das metas teve ações norteadoras iniciadas, mas nenhuma concluída. Ressalta-se que o contexto da pandemia mundial (covid-19) exerceu significativo impacto, interferindo diretamente nos resultados. O estudo apresenta caráter de monitoramento em relação à ferramenta de gestão, tendo em vista a relevância de implementar mecanismos para avaliação sistematizada dos indicadores de desempenho institucionais. Espera-se que as potencialidades percebida...