Albert Lalduhawma Sajem Betlu - Academia.edu (original) (raw)

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Papers by Albert Lalduhawma Sajem Betlu

Figure 1. Distribution of N. khasiana in Meghalaya and Assam (Dima Hasao district).

This report highlights the phytochemical analysis, antioxidant potential and anticancer activity ... more This report highlights the phytochemical analysis, antioxidant potential and anticancer activity against breast carcinoma of 70% methanolic extract of lichen, Parmotrema reticulatum (PRME). Phytochemical analysis of PRME confirms the presence of various phytoconstituents like alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, tannins, anthraquinones, and ascorbic acid; among which alkaloids, phenols and flavonoids are found in abundant amount. High performance liquid chromatography (HPLC) analysis of PRME revealed the presence of catechin, purpurin, tannic acid and reserpine. Antioxidant activity was evaluated by nine separate methods. PRME showed excellent hydroxyl and hypochlorous radical scavenging as well as moderate DPPH, superoxide, singlet oxygen, nitric oxide and peroxynitrite scavenging activity. Cytotoxicity of PRME was tested against breast carcinoma (MCF-7), lung carcinoma (A549) and normal lung fibroblast (WI-38) using WST-1 method. PRME was found cytotoxic against MCF-7 cells with an IC 50 value 130.0363.11 mg/ml while negligible cytotoxicity was observed on A549 and WI-38 cells. Further flow cytometric study showed that PRME halted the MCF-7 cells in S and G2/M phases and induces apoptosis in dose as well as time dependent manner. Cell cycle arrest was associated with downregulation of cyclin B1, Cdk-2 and Cdc25C as well as slight decrease in the expression of Cdk-1 and cyclin A1 with subsequent upregulation of p53 and p21. Moreover PRME induced Bax and inhibited Bcl-2 expression, which results in increasing Bax/Bcl-2 ratio and activation of caspase cascade. This ultimately leads to PARP degradation and induces apoptosis in MCF-7 cells. It can be hypothesised from the current study that the antioxidant and anticancer potential of the PRME may reside in the phytoconstitutents present in it and therefore, PRME may be used as a possible source of natural antioxidant that may be developed to an anticancer agent.

The study of ethnobotany relating to any tribe is in itself a very intricate or convoluted proces... more The study of ethnobotany relating to any tribe is in itself a very intricate or convoluted process. This paper documents the traditional knowledge of medicinal plants that are in use by the indigenous Jaintia tribes residing in few isolated pockets of northeast India. The present study was done through structured questionnaires in consultations with the tribal practitioners and has resulted in the documentation of 39 medicinal plant species belonging to 27 families and 35 genera. For curing diverse form of ailments, the use of aboveground plant parts was higher (76.59%) than the underground plant parts (23.41%). Of the aboveground plant parts, leaf was used in the majority of cases (23 species), followed by fruit (4). Different underground plant forms such as root, tuber, rhizome, bulb and pseudo-bulb were also found to be in use by the Jaintia tribe as a medicine. Altogether, 30 types of ailments have been reported to be cured by using these 39 medicinal plant species. The study thus underlines the potentials of the ethnobotanical research and the need for the documentation of traditional ecological knowledge pertaining to the medicinal plant utilization for the greater benefit of mankind.

The present study addresses the use of zootherapy in the traditional healthcare system of the Bia... more The present study addresses the use of zootherapy in the traditional healthcare system of the Biate tribe of Dima Hasao district, Assam, India. It sought to identify the different species used for zootherapeutic use with the detailed methods of usages to create awareness and contribute to the conservation and sustainable utilization of the resources.

A total of 47 plants species have been reported herein to be used in the treatment of diseases li... more A total of 47 plants species have been reported herein to be used in the treatment of diseases like urinary disorder, diarrhoea, malaria, etc. Among the plant types, herbs species were the most frequently used. Ferns and cycad also find usage in their traditional healing system. Notable among the plants documented is the use of a threatened species like Gloriosa superba as antihelmintics, Cycas revoluta for urinary problems, Elaeagnus caudata for miscarriage, etc. A complete account of species, parts used, mode of preparation and dosage for curing diseases like diarrhoea, malaria, jaundice, diabetes, high blood pressure, snakebite, etc. have been investigated in detail.

Background: Traditional systems of medicine are often a valuable source of novel antimicrobials. ... more Background: Traditional systems of medicine are often a valuable source of novel antimicrobials. Dima Hasao Hill district is endowed with rich cultures of traditional system of medicine and this study is the first of its kind from the area. The aim of the study is to to assess the antimicrobial efficacy of Trema orientalis Blume (Ulmaceae) on six selected bacterial strains. The minimum inhibitory concentrations were determined with the aqueous extract to validate the application of the plant species in traditional medicine.

Traditional use of plants from the Northeastern part of India has been documented for decades' al... more Traditional use of plants from the Northeastern part of India has been documented for decades' altogether. The ethnic community still depend upon their indigenous knowledge for healing their ailments (both intrinsic and extrinsic). The study documents the usage of 31 medicinal plant species belonging to 26 families and 31 genera by the indigenous Lushai tribes of Northeast India. The study was done through structured questionnaires in consultations with the tribal practitioners. The use of aboveground plant parts was higher (79.06%) than the underground plant parts (20.93%). Leaf was used in the majority of cases (23 species), followed by fruit (4). However, different underground plant forms such as root, tuber, rhizome, bulb and pseudo-bulb were also found to be in use by the Lushai tribe as a medicine. About 41 types of ailments have been reported to be cured by using these 31 medicinal plant species. The study thus emphasizes the need to pursue meticulous ethnobotanical research in finding solutions to major fatal diseases and ensure its application in improving human health and medical care facilities.

Figure 1. Distribution of N. khasiana in Meghalaya and Assam (Dima Hasao district).

This report highlights the phytochemical analysis, antioxidant potential and anticancer activity ... more This report highlights the phytochemical analysis, antioxidant potential and anticancer activity against breast carcinoma of 70% methanolic extract of lichen, Parmotrema reticulatum (PRME). Phytochemical analysis of PRME confirms the presence of various phytoconstituents like alkaloids, carbohydrates, flavonoids, glycosides, phenols, saponins, tannins, anthraquinones, and ascorbic acid; among which alkaloids, phenols and flavonoids are found in abundant amount. High performance liquid chromatography (HPLC) analysis of PRME revealed the presence of catechin, purpurin, tannic acid and reserpine. Antioxidant activity was evaluated by nine separate methods. PRME showed excellent hydroxyl and hypochlorous radical scavenging as well as moderate DPPH, superoxide, singlet oxygen, nitric oxide and peroxynitrite scavenging activity. Cytotoxicity of PRME was tested against breast carcinoma (MCF-7), lung carcinoma (A549) and normal lung fibroblast (WI-38) using WST-1 method. PRME was found cytotoxic against MCF-7 cells with an IC 50 value 130.0363.11 mg/ml while negligible cytotoxicity was observed on A549 and WI-38 cells. Further flow cytometric study showed that PRME halted the MCF-7 cells in S and G2/M phases and induces apoptosis in dose as well as time dependent manner. Cell cycle arrest was associated with downregulation of cyclin B1, Cdk-2 and Cdc25C as well as slight decrease in the expression of Cdk-1 and cyclin A1 with subsequent upregulation of p53 and p21. Moreover PRME induced Bax and inhibited Bcl-2 expression, which results in increasing Bax/Bcl-2 ratio and activation of caspase cascade. This ultimately leads to PARP degradation and induces apoptosis in MCF-7 cells. It can be hypothesised from the current study that the antioxidant and anticancer potential of the PRME may reside in the phytoconstitutents present in it and therefore, PRME may be used as a possible source of natural antioxidant that may be developed to an anticancer agent.

The study of ethnobotany relating to any tribe is in itself a very intricate or convoluted proces... more The study of ethnobotany relating to any tribe is in itself a very intricate or convoluted process. This paper documents the traditional knowledge of medicinal plants that are in use by the indigenous Jaintia tribes residing in few isolated pockets of northeast India. The present study was done through structured questionnaires in consultations with the tribal practitioners and has resulted in the documentation of 39 medicinal plant species belonging to 27 families and 35 genera. For curing diverse form of ailments, the use of aboveground plant parts was higher (76.59%) than the underground plant parts (23.41%). Of the aboveground plant parts, leaf was used in the majority of cases (23 species), followed by fruit (4). Different underground plant forms such as root, tuber, rhizome, bulb and pseudo-bulb were also found to be in use by the Jaintia tribe as a medicine. Altogether, 30 types of ailments have been reported to be cured by using these 39 medicinal plant species. The study thus underlines the potentials of the ethnobotanical research and the need for the documentation of traditional ecological knowledge pertaining to the medicinal plant utilization for the greater benefit of mankind.

The present study addresses the use of zootherapy in the traditional healthcare system of the Bia... more The present study addresses the use of zootherapy in the traditional healthcare system of the Biate tribe of Dima Hasao district, Assam, India. It sought to identify the different species used for zootherapeutic use with the detailed methods of usages to create awareness and contribute to the conservation and sustainable utilization of the resources.

A total of 47 plants species have been reported herein to be used in the treatment of diseases li... more A total of 47 plants species have been reported herein to be used in the treatment of diseases like urinary disorder, diarrhoea, malaria, etc. Among the plant types, herbs species were the most frequently used. Ferns and cycad also find usage in their traditional healing system. Notable among the plants documented is the use of a threatened species like Gloriosa superba as antihelmintics, Cycas revoluta for urinary problems, Elaeagnus caudata for miscarriage, etc. A complete account of species, parts used, mode of preparation and dosage for curing diseases like diarrhoea, malaria, jaundice, diabetes, high blood pressure, snakebite, etc. have been investigated in detail.

Background: Traditional systems of medicine are often a valuable source of novel antimicrobials. ... more Background: Traditional systems of medicine are often a valuable source of novel antimicrobials. Dima Hasao Hill district is endowed with rich cultures of traditional system of medicine and this study is the first of its kind from the area. The aim of the study is to to assess the antimicrobial efficacy of Trema orientalis Blume (Ulmaceae) on six selected bacterial strains. The minimum inhibitory concentrations were determined with the aqueous extract to validate the application of the plant species in traditional medicine.

Traditional use of plants from the Northeastern part of India has been documented for decades' al... more Traditional use of plants from the Northeastern part of India has been documented for decades' altogether. The ethnic community still depend upon their indigenous knowledge for healing their ailments (both intrinsic and extrinsic). The study documents the usage of 31 medicinal plant species belonging to 26 families and 31 genera by the indigenous Lushai tribes of Northeast India. The study was done through structured questionnaires in consultations with the tribal practitioners. The use of aboveground plant parts was higher (79.06%) than the underground plant parts (20.93%). Leaf was used in the majority of cases (23 species), followed by fruit (4). However, different underground plant forms such as root, tuber, rhizome, bulb and pseudo-bulb were also found to be in use by the Lushai tribe as a medicine. About 41 types of ailments have been reported to be cured by using these 31 medicinal plant species. The study thus emphasizes the need to pursue meticulous ethnobotanical research in finding solutions to major fatal diseases and ensure its application in improving human health and medical care facilities.