Amiza Mat Amin - Academia.edu (original) (raw)
Papers by Amiza Mat Amin
Research Journal of Microbiology, 2006
Pyropia vietnamensis is one of the most important and abundant seaweed in Indo-Pacific region. Ho... more Pyropia vietnamensis is one of the most important and abundant seaweed in Indo-Pacific region. However, no study has been reported on the biotechnological utilization of this species. This study aimed to carry out in silico evaluation of P. vietnamensis proteins as potential precursors of bioactive peptides and to determine the most efficient proteolytic enzymes to release the bioactive peptides. In the present study, five main proteins from P. vietnamensis were chosen and analysed via in silico approach using the BIOPEP-UWM database. It was found that dipeptidyl peptidase-IV (DPP-IV) inhibitors and angiotensin-I converting enzyme (ACE) inhibitors were the most potential bioactive peptides released from P. vietnamensis proteins. Seven enzymes (pancreatic elastaste, papain, ficin, leukocyte elastaste, stem bromelain, calpain 2, and pepsin (pH>2)) were then employed for in silico proteolysis to release both dominant bioactivities. Pepsin (pH>2) and calpain 2 were found to be eff...
Journal of Food Science and Technology, 2014
This study aimed to determine the effects of 2-5 wash cycles and the addition of tetrasodium pyro... more This study aimed to determine the effects of 2-5 wash cycles and the addition of tetrasodium pyrophosphate (TSPP) (0 %, 0.05 Surimi% and 0.1 % w/w)-with or without the addition of 0.4 % calcium chloride (CaCl 2)-on the physical properties such as texture, colour, expressible moisture and microstructure of Cobia (Rachycentron canadum) surimi gel. The highest breaking force (484.85 g) was obtained with the addition 0.1 % TSPP alone on the fifth wash. However, a combination of 0.1 and 0.4 % CaCl 2 in surimi gels at wash cycle 5 resulted in the highest degree of whiteness (86.8 %), as well as total expressible moisture (2.785 %) and deformation (17.11 mm). The highest surimi gel strength (6,923 g.mm) was obtained after three wash cycles with the addition of 0.1 % TSPP +0.4 % CaCl 2. The physical properties of Cobia fish surimi gels were affected by the number of wash cycles and treatments with TSPP and CaCl 2 .
Universiti Malaysia Terengganu Journal of Undergraduate Research
This study aimed to determine the physicochemical properties of undulated surf clam (Paphia undul... more This study aimed to determine the physicochemical properties of undulated surf clam (Paphia undulata) hydrolysate as affected by the degree of hydrolysis (DH). Three levels of DH of undulated surf clam hydrolysate were prepared which were DH 36.57% (without any enzymatic hydrolysis), DH 58.25% (0.5% Alcalase®; 5 min; pH 7.5; 60ºC) and DH 91.26% (1% Alcalase®; 30 min; pH 7.5; 60ºC). After protein hydrolysis, the undulated surf clam hydrolysates were centrifuged, and their supernatants were freeze-dried. This study found that the protein hydrolysate with lower DH (DH 36.57%) gave lower protein content and higher ash and fat contents compared to other samples (DH 58.25% and DH 91.26%). However, the carbohydrate content is similar in all samples (16.56-20.04%). This study also found that foaming properties (29.43-67.50%), emulsifying capacity (11.94-110.52%) and peptide solubility (57.61-94.08%) were affected by the DH. As DH increased, the emulsifying capacity decreased, while peptide ...
Malaysian Applied Biology
The present study aimed to perform an in silico evaluation of edible bird’s nest protein as poten... more The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study were chosen as potential precursors to produce bioactive peptides via in silico method using the BIOPEP database. AMCase protein sequences gave the highest number of bioactivities (16 to 18) and nucleobindin-2 protein gave the lowest number of bioactivities (9) among the other protein sequences. It was found that the most potential bioactive peptides from EBN proteins are angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-IV (DPPIV) inhibitors. Furthermore, in silico proteolysis using six selected enzymes was employed to release both dominant bioactivities in EBN proteins, which were ACE and DPP-IV inhibitors. This study shows that a combination of enzymes, chymotryps...
Penerbit Universiti Kebangsaan Malaysia, Jun 1, 2019
This study reported on the optimization of enzymatic hydrolysis condition of edible bird's nest (... more This study reported on the optimization of enzymatic hydrolysis condition of edible bird's nest (EBN) using Protamex® to obtain maximum degree of hydrolysis degree (DH). Besides, the proximate analyses of soaked cleaned raw EBN and its lyophilized hydrolysate powder prepared under optimum condition (suggested in this study) were also compared. Response surface methodology (RSM) was employed using a threelevel face-centered Central Composite Design (CCD) at four different parameters, namely temperature (40-60 o C), concentration of Protamex® (0.5-1.5%), pH (5.5-9.5) and hydrolysis time (2-6 hr). It was found that a quadratic fit could explain the effect of these four variables on the DH of EBN. The optimum condition was obtained at temperature 59.9°C, pH of 6.3, Protamex ® concentration of 2% and hydrolysis time of 5.4 hr. The DH achieved under this optimum condition (33.88%) was close to the predicted DH (34.11%). It was found that the lyophilized EBN hydrolysate powder prepared under the optimum condition gave similar protein and carbohydrate content, but lower fat content and higher ash content as compared to cleaned raw EBN.
Malaysian Applied Biology, 2020
The main objective of this study was to determine effect of different heat pretreatments on chemi... more The main objective of this study was to determine effect of different heat pretreatments on chemical and antioxidant properties of Melon Manis Terengganu (MMT) seed oil. The seeds were treated with four different heat pretreatment which were untreated (control), roasting, steaming and microwaving. Then the MMT seed oil was extracted from each treated seed using conventional extraction method. The chemical tests determined on the MMT seed oil were free fatty acid (FFA), iodine value (IV), saponification value and fatty acid composition. The antioxidant properties for MMT seed oil evaluated was total phenolic content (TPC), 1,1- diphenyl-2-picrylhdrazyl (DPPH) scavenging capacity and Ferric reducing antioxidant power (FRAP). It was found that different heat pretreatments of MMT seed significantly (p<0.05) affected the oil yield (11.10–15.40%.), free fatty acid value (3.55–5.04%), DPPH radical scavenging activity (43.06–56.08%), total phenolic content (0.088–0.142 mg GAE/g) and FRAP...
Optimisation is commonly employed to find the conditions that produce the best possible response,... more Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud crab meat using RSM by employing a face-centered Central Composite Design (CCD) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity. Screening of commercial food grade proteinases was carried out using Alcalase®, Protamex®, Neutrase® and papain shows that Protamex® gave the highest ACEI activity. The enzymatic hydrolysis conditions used in the optimisation were temperature (45-65oC), pH (pH 5.5-7.5), hydrolysis time (1-4 hours) and Protamex® enzyme to substrate (E/S) ratio (1-3%). It was found that quadratic model was able to explain the relationship between the hydrolysis conditions and ACEI activity. The optimum conditions were obtained at 65oC, pH 5.6, 1% E/S rati...
In this study, optimization of enzymatic protein hydrolysis conditions of flower crab meat (FCM) ... more In this study, optimization of enzymatic protein hydrolysis conditions of flower crab meat (FCM) to yield maximum angiotensin converting enzyme (ACE) inhibitory activity was carried out. First, screening of commercial food grade enzymes (Alcalase®, Neutrase®, Protamex® and papain) was carried out to select the most suitable proteinase to yield ACE inhibitory activity. A 3-level face-centered central composite design (CCD) was employed to optimize four hydrolysis conditions including temperature (45-55°C), hydrolysis time (1-3 hr), pH (6-8) and enzyme to substrate ratio (E/S) (1-3%). Half maximal inhibitory concentration (IC50) of FCM hydrolysate prepared at optimum condition was also determined. It was found that the highest ACE inhibitory activity (85.52%) was given by Neutrase® after 2 hr hydrolysis. Hence, Neutrase® was used in the optimization study. It was found that the enzymatic hydrolysis condition of FCM towards ACE inhibitory activity could be predicted by a quadratic mode...
The aim of this study was to determine the effects of turmeric powder, chilli powder and sodium a... more The aim of this study was to determine the effects of turmeric powder, chilli powder and sodium acetate on the shelf life of refrigerated tilapia fillets at 5oC. Six treatments were applied to the tilapia fillets which were salt (S), salt and turmeric (ST), salt and chilli (SC), salt, turmeric and sodium acetate (STNaA) and salt, chilli and sodium acetate (SCNaA) and control. The samples were analyzed in 15 days period for microbiological, chemical and physical analyses. It was found that there were significant differences (p<0.05) in all microbiological analysis of fish treated with STNaA and SCNaA compared to other treatments. Microbiologically, the shelf life of fillets treated with STNaA and SCNaA was 12 days, fillets treated with ST, SC and S were 6 days, and only 3 days for control fillet. In terms of TVB-N and TMA values, ST fillets were still acceptable up to 12 to 15 days of refrigerated storage. As for TBA values, they were in acceptable range for all treatments through...
Food Research, 2020
Although the fried products are delicious with a tenderizing effect on the crust due to the prese... more Although the fried products are delicious with a tenderizing effect on the crust due to the presence of fat, over-consumption of fried products causes health problems, especially coronary diseases. The tendency of proteins in film formation and thermal gelation to reduce the absorption of oil in fried products is emphasized. The purpose of this study was to determine the reduction of oil in deep-fried squid by the incorporation of protein hydrolysate and to discuss its effect. Yellowstripe scad protein hydrolysate was produced using Alcalase® enzyme. Fat content was determined using the Soxhlet method, subsequently substituted into a formulation for oil uptake calculation. The viscosity of batter was determined using a rheometer. The viscosity of the batter and batter pick-up was found to be directly proportional, showing a significantly reduced pattern from 0 to 20%. Incorporating 10% of fish protein hydrolysate successfully decreased oil absorption by 17.35±0.73% with a good water...
Madridge Journal of Food Technology, 2017
Journal of Biological Sciences, 2002
... 9): 591-595, 2002 ISSN 1608-4127 A Single Step Purification of Gastricsin-Like Proteinase fro... more ... 9): 591-595, 2002 ISSN 1608-4127 A Single Step Purification of Gastricsin-Like Proteinase from Atlantic Cod (Gadus morhua) &#x27;MA Amiza and RK Owusu Apenten 1 Department of Food Science, Faculty of Science and Technology, Kolej Universiti Sains dan Teknologi Malaysia ...
Journal of Biological Sciences, 2004
Journal of the Science of Food and Agriculture, 1994
ABSTRACT The heat inactivation of a mixture of alkaline proteinases from North Sea cod (Gadus mor... more ABSTRACT The heat inactivation of a mixture of alkaline proteinases from North Sea cod (Gadus morhua) viscera and bovine α-chymotrypsin were compared at 40–60°C, pH 8.0. Fish digestive proteinases were at least 10-fold less heat resistant than chymotrypsin mainly as a result of a lower apparent activation enthalpy (ΔH#) for the thermoinactivation of the former proteinases.
Journal of Food Biochemistry, 1997
i'ke heat inactivation of cod (Gadus morhua) trypsin was examined at 40-55C by nonlinear regressi... more i'ke heat inactivation of cod (Gadus morhua) trypsin was examined at 40-55C by nonlinear regression (NU) analysis according to 1 st-order, n-th order, or consecutive reaction kinetics. At 40C Cod trypsin was inactivated by a 2nd-order reaction; n = 2 (fO.014) with a pseudo rate constant (k') of 6.46 (20.04) X (s-'). The order of reaction decreased with increasing temperature; n = 1.54 (f0.003) at45C, n = 0.8 (*0.005) at50Corn = 0.64 (f0.014) at5SC. At 45-55C the consecutive model also indicated 1st-order inactivation kinetics. SDS-PAGE analysis showed that with progressive heating the concentration of cod trypsin decreased and there was an appearance of lower molecular weight polypeptides. The results are consistent with a heat inactivation process involving trypsin autolysis. A mechanisrti for cod trypsin heat inactivation is proposed which accounts for the observed 1 st-order or 2nd-order reaction kinetics as limiting cases.
Alternative Assessments in Malaysian Higher Education, 2022
Penerbit Universiti Kebangsaan Malaysia, Dec 31, 2020
This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by... more This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by using Alcalase® to obtain maximum angiotensin-I-converting enzyme (ACE) inhibitory activity. Firstly, the seaweed protein was extracted using cellulase, sonication, and ammonium sulphate treatment, before dialysis and lyophilization. The yield of lyophilized seaweed protein extract was 8.75% with a protein content of 66.4%. An optimization study for protein hydrolysis condition was performed by employing a three-level face-centered central composite design (CCD) using Design-Expert software. Four parameters used were pH (6.5-8.5), temperature (50-60°C), hydrolysis time (60-180 min), and Alcalase® to substrate ratio (E/S) (1.25-2.50%). Thirty runs of protein hydrolysis conditions with 6 center points were employed. The supernatant of the resulting protein hydrolysates was then lyophilized and analyzed for ACE inhibitory activity. This study found that the quadratic model could be used to explain the relationship between hydrolysis conditions of G. fisheri protein and ACE inhibitory activity. The optimum condition to obtain maximum ACE inhibitory activity was at pH of 7.5, the temperature of 54.6°C, hydrolysis time of 175 min, and E/S of 1.47%. The half-maximal inhibitory concentration (IC 50) of the seaweed protein hydrolysate at optimum condition was 2.97 ± 0.37 mg/mL.
Malaysian Applied Biology, 2020
This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by... more This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by using Alcalase® to obtain maximum angiotensin-I-converting enzyme (ACE) inhibitory activity. Firstly, the seaweed protein was extracted using cellulase, sonication, and ammonium sulphate treatment, before dialysis and lyophilization. The yield of lyophilized seaweed protein extract was 8.75% with a protein content of 66.4%. An optimization study for protein hydrolysis condition was performed by employing a three-level face-centered central composite design (CCD) using Design-Expert software. Four parameters used were pH (6.5 – 8.5), temperature (50 – 60°C), hydrolysis time (60 – 180 min), and Alcalase® to substrate ratio (E/S) (1.25 – 2.50%). Thirty runs of protein hydrolysis conditions with 6 center points were employed. The supernatant of the resulting protein hydrolysates was then lyophilized and analyzed for ACE inhibitory activity. This study found that the quadratic model could be...
Malaysian Applied Biology
Blood cockle (Anadara granosa) is the most abundant and available bivalves in Malaysia. Blood coc... more Blood cockle (Anadara granosa) is the most abundant and available bivalves in Malaysia. Blood cockles meat has high protein content and has potential to generate bioactive peptides. To date, no study has been reported on purification and identification of angiotensin I converting enzyme (ACE) inhibitory peptides from blood cockle meat. Thus, the objectives of this study were to purify and characterize ACE inhibitory peptide from blood cockle meat hydrolysate. ACE inhibitory peptides from blood cockle meat hydrolysate (CMH) were prepared by enzymatic protein hydrolysis using Protamex®. Crude CMH was characterized for its stability against gastrointestinal proteases, at varying pH (2–11) and temperature (4–90°C). Next, crude CMH was purified by ultrafiltration, ion exchange chromatography and reverse-phase chromatography and its amino acid sequence was identified. It was found that crude CMH was highly stable at low pH and temperature, and was resistant to gastrointestinal proteases (...
Research Journal of Microbiology, 2006
Pyropia vietnamensis is one of the most important and abundant seaweed in Indo-Pacific region. Ho... more Pyropia vietnamensis is one of the most important and abundant seaweed in Indo-Pacific region. However, no study has been reported on the biotechnological utilization of this species. This study aimed to carry out in silico evaluation of P. vietnamensis proteins as potential precursors of bioactive peptides and to determine the most efficient proteolytic enzymes to release the bioactive peptides. In the present study, five main proteins from P. vietnamensis were chosen and analysed via in silico approach using the BIOPEP-UWM database. It was found that dipeptidyl peptidase-IV (DPP-IV) inhibitors and angiotensin-I converting enzyme (ACE) inhibitors were the most potential bioactive peptides released from P. vietnamensis proteins. Seven enzymes (pancreatic elastaste, papain, ficin, leukocyte elastaste, stem bromelain, calpain 2, and pepsin (pH>2)) were then employed for in silico proteolysis to release both dominant bioactivities. Pepsin (pH>2) and calpain 2 were found to be eff...
Journal of Food Science and Technology, 2014
This study aimed to determine the effects of 2-5 wash cycles and the addition of tetrasodium pyro... more This study aimed to determine the effects of 2-5 wash cycles and the addition of tetrasodium pyrophosphate (TSPP) (0 %, 0.05 Surimi% and 0.1 % w/w)-with or without the addition of 0.4 % calcium chloride (CaCl 2)-on the physical properties such as texture, colour, expressible moisture and microstructure of Cobia (Rachycentron canadum) surimi gel. The highest breaking force (484.85 g) was obtained with the addition 0.1 % TSPP alone on the fifth wash. However, a combination of 0.1 and 0.4 % CaCl 2 in surimi gels at wash cycle 5 resulted in the highest degree of whiteness (86.8 %), as well as total expressible moisture (2.785 %) and deformation (17.11 mm). The highest surimi gel strength (6,923 g.mm) was obtained after three wash cycles with the addition of 0.1 % TSPP +0.4 % CaCl 2. The physical properties of Cobia fish surimi gels were affected by the number of wash cycles and treatments with TSPP and CaCl 2 .
Universiti Malaysia Terengganu Journal of Undergraduate Research
This study aimed to determine the physicochemical properties of undulated surf clam (Paphia undul... more This study aimed to determine the physicochemical properties of undulated surf clam (Paphia undulata) hydrolysate as affected by the degree of hydrolysis (DH). Three levels of DH of undulated surf clam hydrolysate were prepared which were DH 36.57% (without any enzymatic hydrolysis), DH 58.25% (0.5% Alcalase®; 5 min; pH 7.5; 60ºC) and DH 91.26% (1% Alcalase®; 30 min; pH 7.5; 60ºC). After protein hydrolysis, the undulated surf clam hydrolysates were centrifuged, and their supernatants were freeze-dried. This study found that the protein hydrolysate with lower DH (DH 36.57%) gave lower protein content and higher ash and fat contents compared to other samples (DH 58.25% and DH 91.26%). However, the carbohydrate content is similar in all samples (16.56-20.04%). This study also found that foaming properties (29.43-67.50%), emulsifying capacity (11.94-110.52%) and peptide solubility (57.61-94.08%) were affected by the DH. As DH increased, the emulsifying capacity decreased, while peptide ...
Malaysian Applied Biology
The present study aimed to perform an in silico evaluation of edible bird’s nest protein as poten... more The present study aimed to perform an in silico evaluation of edible bird’s nest protein as potential precursors of bioactive peptides, as well as to determine whether such peptides can be released by selected proteolytic enzymes. Six edible bird’s nest (EBN) protein sequences from a previous study were chosen as potential precursors to produce bioactive peptides via in silico method using the BIOPEP database. AMCase protein sequences gave the highest number of bioactivities (16 to 18) and nucleobindin-2 protein gave the lowest number of bioactivities (9) among the other protein sequences. It was found that the most potential bioactive peptides from EBN proteins are angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-IV (DPPIV) inhibitors. Furthermore, in silico proteolysis using six selected enzymes was employed to release both dominant bioactivities in EBN proteins, which were ACE and DPP-IV inhibitors. This study shows that a combination of enzymes, chymotryps...
Penerbit Universiti Kebangsaan Malaysia, Jun 1, 2019
This study reported on the optimization of enzymatic hydrolysis condition of edible bird's nest (... more This study reported on the optimization of enzymatic hydrolysis condition of edible bird's nest (EBN) using Protamex® to obtain maximum degree of hydrolysis degree (DH). Besides, the proximate analyses of soaked cleaned raw EBN and its lyophilized hydrolysate powder prepared under optimum condition (suggested in this study) were also compared. Response surface methodology (RSM) was employed using a threelevel face-centered Central Composite Design (CCD) at four different parameters, namely temperature (40-60 o C), concentration of Protamex® (0.5-1.5%), pH (5.5-9.5) and hydrolysis time (2-6 hr). It was found that a quadratic fit could explain the effect of these four variables on the DH of EBN. The optimum condition was obtained at temperature 59.9°C, pH of 6.3, Protamex ® concentration of 2% and hydrolysis time of 5.4 hr. The DH achieved under this optimum condition (33.88%) was close to the predicted DH (34.11%). It was found that the lyophilized EBN hydrolysate powder prepared under the optimum condition gave similar protein and carbohydrate content, but lower fat content and higher ash content as compared to cleaned raw EBN.
Malaysian Applied Biology, 2020
The main objective of this study was to determine effect of different heat pretreatments on chemi... more The main objective of this study was to determine effect of different heat pretreatments on chemical and antioxidant properties of Melon Manis Terengganu (MMT) seed oil. The seeds were treated with four different heat pretreatment which were untreated (control), roasting, steaming and microwaving. Then the MMT seed oil was extracted from each treated seed using conventional extraction method. The chemical tests determined on the MMT seed oil were free fatty acid (FFA), iodine value (IV), saponification value and fatty acid composition. The antioxidant properties for MMT seed oil evaluated was total phenolic content (TPC), 1,1- diphenyl-2-picrylhdrazyl (DPPH) scavenging capacity and Ferric reducing antioxidant power (FRAP). It was found that different heat pretreatments of MMT seed significantly (p<0.05) affected the oil yield (11.10–15.40%.), free fatty acid value (3.55–5.04%), DPPH radical scavenging activity (43.06–56.08%), total phenolic content (0.088–0.142 mg GAE/g) and FRAP...
Optimisation is commonly employed to find the conditions that produce the best possible response,... more Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud crab meat using RSM by employing a face-centered Central Composite Design (CCD) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity. Screening of commercial food grade proteinases was carried out using Alcalase®, Protamex®, Neutrase® and papain shows that Protamex® gave the highest ACEI activity. The enzymatic hydrolysis conditions used in the optimisation were temperature (45-65oC), pH (pH 5.5-7.5), hydrolysis time (1-4 hours) and Protamex® enzyme to substrate (E/S) ratio (1-3%). It was found that quadratic model was able to explain the relationship between the hydrolysis conditions and ACEI activity. The optimum conditions were obtained at 65oC, pH 5.6, 1% E/S rati...
In this study, optimization of enzymatic protein hydrolysis conditions of flower crab meat (FCM) ... more In this study, optimization of enzymatic protein hydrolysis conditions of flower crab meat (FCM) to yield maximum angiotensin converting enzyme (ACE) inhibitory activity was carried out. First, screening of commercial food grade enzymes (Alcalase®, Neutrase®, Protamex® and papain) was carried out to select the most suitable proteinase to yield ACE inhibitory activity. A 3-level face-centered central composite design (CCD) was employed to optimize four hydrolysis conditions including temperature (45-55°C), hydrolysis time (1-3 hr), pH (6-8) and enzyme to substrate ratio (E/S) (1-3%). Half maximal inhibitory concentration (IC50) of FCM hydrolysate prepared at optimum condition was also determined. It was found that the highest ACE inhibitory activity (85.52%) was given by Neutrase® after 2 hr hydrolysis. Hence, Neutrase® was used in the optimization study. It was found that the enzymatic hydrolysis condition of FCM towards ACE inhibitory activity could be predicted by a quadratic mode...
The aim of this study was to determine the effects of turmeric powder, chilli powder and sodium a... more The aim of this study was to determine the effects of turmeric powder, chilli powder and sodium acetate on the shelf life of refrigerated tilapia fillets at 5oC. Six treatments were applied to the tilapia fillets which were salt (S), salt and turmeric (ST), salt and chilli (SC), salt, turmeric and sodium acetate (STNaA) and salt, chilli and sodium acetate (SCNaA) and control. The samples were analyzed in 15 days period for microbiological, chemical and physical analyses. It was found that there were significant differences (p<0.05) in all microbiological analysis of fish treated with STNaA and SCNaA compared to other treatments. Microbiologically, the shelf life of fillets treated with STNaA and SCNaA was 12 days, fillets treated with ST, SC and S were 6 days, and only 3 days for control fillet. In terms of TVB-N and TMA values, ST fillets were still acceptable up to 12 to 15 days of refrigerated storage. As for TBA values, they were in acceptable range for all treatments through...
Food Research, 2020
Although the fried products are delicious with a tenderizing effect on the crust due to the prese... more Although the fried products are delicious with a tenderizing effect on the crust due to the presence of fat, over-consumption of fried products causes health problems, especially coronary diseases. The tendency of proteins in film formation and thermal gelation to reduce the absorption of oil in fried products is emphasized. The purpose of this study was to determine the reduction of oil in deep-fried squid by the incorporation of protein hydrolysate and to discuss its effect. Yellowstripe scad protein hydrolysate was produced using Alcalase® enzyme. Fat content was determined using the Soxhlet method, subsequently substituted into a formulation for oil uptake calculation. The viscosity of batter was determined using a rheometer. The viscosity of the batter and batter pick-up was found to be directly proportional, showing a significantly reduced pattern from 0 to 20%. Incorporating 10% of fish protein hydrolysate successfully decreased oil absorption by 17.35±0.73% with a good water...
Madridge Journal of Food Technology, 2017
Journal of Biological Sciences, 2002
... 9): 591-595, 2002 ISSN 1608-4127 A Single Step Purification of Gastricsin-Like Proteinase fro... more ... 9): 591-595, 2002 ISSN 1608-4127 A Single Step Purification of Gastricsin-Like Proteinase from Atlantic Cod (Gadus morhua) &#x27;MA Amiza and RK Owusu Apenten 1 Department of Food Science, Faculty of Science and Technology, Kolej Universiti Sains dan Teknologi Malaysia ...
Journal of Biological Sciences, 2004
Journal of the Science of Food and Agriculture, 1994
ABSTRACT The heat inactivation of a mixture of alkaline proteinases from North Sea cod (Gadus mor... more ABSTRACT The heat inactivation of a mixture of alkaline proteinases from North Sea cod (Gadus morhua) viscera and bovine α-chymotrypsin were compared at 40–60°C, pH 8.0. Fish digestive proteinases were at least 10-fold less heat resistant than chymotrypsin mainly as a result of a lower apparent activation enthalpy (ΔH#) for the thermoinactivation of the former proteinases.
Journal of Food Biochemistry, 1997
i'ke heat inactivation of cod (Gadus morhua) trypsin was examined at 40-55C by nonlinear regressi... more i'ke heat inactivation of cod (Gadus morhua) trypsin was examined at 40-55C by nonlinear regression (NU) analysis according to 1 st-order, n-th order, or consecutive reaction kinetics. At 40C Cod trypsin was inactivated by a 2nd-order reaction; n = 2 (fO.014) with a pseudo rate constant (k') of 6.46 (20.04) X (s-'). The order of reaction decreased with increasing temperature; n = 1.54 (f0.003) at45C, n = 0.8 (*0.005) at50Corn = 0.64 (f0.014) at5SC. At 45-55C the consecutive model also indicated 1st-order inactivation kinetics. SDS-PAGE analysis showed that with progressive heating the concentration of cod trypsin decreased and there was an appearance of lower molecular weight polypeptides. The results are consistent with a heat inactivation process involving trypsin autolysis. A mechanisrti for cod trypsin heat inactivation is proposed which accounts for the observed 1 st-order or 2nd-order reaction kinetics as limiting cases.
Alternative Assessments in Malaysian Higher Education, 2022
Penerbit Universiti Kebangsaan Malaysia, Dec 31, 2020
This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by... more This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by using Alcalase® to obtain maximum angiotensin-I-converting enzyme (ACE) inhibitory activity. Firstly, the seaweed protein was extracted using cellulase, sonication, and ammonium sulphate treatment, before dialysis and lyophilization. The yield of lyophilized seaweed protein extract was 8.75% with a protein content of 66.4%. An optimization study for protein hydrolysis condition was performed by employing a three-level face-centered central composite design (CCD) using Design-Expert software. Four parameters used were pH (6.5-8.5), temperature (50-60°C), hydrolysis time (60-180 min), and Alcalase® to substrate ratio (E/S) (1.25-2.50%). Thirty runs of protein hydrolysis conditions with 6 center points were employed. The supernatant of the resulting protein hydrolysates was then lyophilized and analyzed for ACE inhibitory activity. This study found that the quadratic model could be used to explain the relationship between hydrolysis conditions of G. fisheri protein and ACE inhibitory activity. The optimum condition to obtain maximum ACE inhibitory activity was at pH of 7.5, the temperature of 54.6°C, hydrolysis time of 175 min, and E/S of 1.47%. The half-maximal inhibitory concentration (IC 50) of the seaweed protein hydrolysate at optimum condition was 2.97 ± 0.37 mg/mL.
Malaysian Applied Biology, 2020
This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by... more This study aimed to optimize the enzymatic hydrolysis conditions of Gracilaria fisheri protein by using Alcalase® to obtain maximum angiotensin-I-converting enzyme (ACE) inhibitory activity. Firstly, the seaweed protein was extracted using cellulase, sonication, and ammonium sulphate treatment, before dialysis and lyophilization. The yield of lyophilized seaweed protein extract was 8.75% with a protein content of 66.4%. An optimization study for protein hydrolysis condition was performed by employing a three-level face-centered central composite design (CCD) using Design-Expert software. Four parameters used were pH (6.5 – 8.5), temperature (50 – 60°C), hydrolysis time (60 – 180 min), and Alcalase® to substrate ratio (E/S) (1.25 – 2.50%). Thirty runs of protein hydrolysis conditions with 6 center points were employed. The supernatant of the resulting protein hydrolysates was then lyophilized and analyzed for ACE inhibitory activity. This study found that the quadratic model could be...
Malaysian Applied Biology
Blood cockle (Anadara granosa) is the most abundant and available bivalves in Malaysia. Blood coc... more Blood cockle (Anadara granosa) is the most abundant and available bivalves in Malaysia. Blood cockles meat has high protein content and has potential to generate bioactive peptides. To date, no study has been reported on purification and identification of angiotensin I converting enzyme (ACE) inhibitory peptides from blood cockle meat. Thus, the objectives of this study were to purify and characterize ACE inhibitory peptide from blood cockle meat hydrolysate. ACE inhibitory peptides from blood cockle meat hydrolysate (CMH) were prepared by enzymatic protein hydrolysis using Protamex®. Crude CMH was characterized for its stability against gastrointestinal proteases, at varying pH (2–11) and temperature (4–90°C). Next, crude CMH was purified by ultrafiltration, ion exchange chromatography and reverse-phase chromatography and its amino acid sequence was identified. It was found that crude CMH was highly stable at low pH and temperature, and was resistant to gastrointestinal proteases (...