Amy Britton - Academia.edu (original) (raw)
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Centre National de la Recherche Scientifique / French National Centre for Scientific Research
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Papers by Amy Britton
FEBS Open Bio, 2020
Clostridium botulinum neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcho... more Clostridium botulinum neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcholine release from motor neurons; however, at tiny doses, this property is exploited for use as a therapeutic. Each member of the BoNT family of proteins consists of three distinct domains: a binding domain that targets neuronal cell membranes (H C), a translocation domain (H N), and a catalytic domain (LC). Here we present high-resolution crystal structures of the binding domains of BoNT subtypes /A5 (H C /A5) and /A6 (H C /A6). These structures show that the core fold identified in other subtypes is maintained, but with subtle differences at the expected receptor binding sites.
FEBS Open Bio, 2020
Clostridium botulinum neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcho... more Clostridium botulinum neurotoxins (BoNTs) cause flaccid paralysis through inhibition of acetylcholine release from motor neurons; however, at tiny doses, this property is exploited for use as a therapeutic. Each member of the BoNT family of proteins consists of three distinct domains: a binding domain that targets neuronal cell membranes (H C), a translocation domain (H N), and a catalytic domain (LC). Here we present high-resolution crystal structures of the binding domains of BoNT subtypes /A5 (H C /A5) and /A6 (H C /A6). These structures show that the core fold identified in other subtypes is maintained, but with subtle differences at the expected receptor binding sites.