André Hazout - Academia.edu (original) (raw)
Papers by André Hazout
Genesis, 1995
Peer reviewe
Human Reproduction, 1988
LHRH agonists are being increasingly used in ovulation stimulation protocols in IVF programmes. W... more LHRH agonists are being increasingly used in ovulation stimulation protocols in IVF programmes. We have compared the results of two methods of utilization of LHRH agonists. In the long protocol, gonadotrophin stimulation was only commenced after a preliminary period of pituitary desensitization with LHRH agonist. In the short protocol, exogenous gonadotrophins were administered shortly after the start of LHRH agonist therapy, benefiting from the gonadotrophin flare-up effect. One-hundred-and-eighty-six patients were divided equally between the two treatments. There was no difference in the ovarian response on the day of HCG or the number of mature oocytes recovered. The cleavage rate of mature oocytes was higher in the short protocol (70, versus 56, P less than 0.01). The ongoing pregnancy rate per treatment cycle was similar in both groups (18, in the long protocol and 16, in the short protocol). Analysis of the luteal phases revealed a trend for higher progesterone values in the long protocol although this was only significant on the second day following oocyte retrieval. As the clinical results were similar other factors should be taken into account when deciding therapy. These include patient convenience, cost and side-effects. Other schedules of ovulation stimulation using LHRH agonists are discussed.
The present invention pertains to the use of DNase to treat female infertility and the use of cfD... more The present invention pertains to the use of DNase to treat female infertility and the use of cfDNA as a marker of female infertility.
Gynécologie Obstétrique & Fertilité, 2006
Gynécologie Obstétrique & Fertilité, 2007
Gynécologie, obstétrique & fertilité, 2008
Numerous recent studies involve DNA damages associated with poor fertilization rates, early embry... more Numerous recent studies involve DNA damages associated with poor fertilization rates, early embryo development defect, and poor quality of conceptus following Assisted Reproductive Technologies (ART). The authors denounce a particularly high rate of miscarriages and childhood cancer or dominant genetic mutations such as achondroplasia, Apert syndrome or aberrant gene imprinting such as Angelman and Beckwith Wiedeman syndromes. Gametes DNA defects have numerous origins which are difficult to determine; they are known to involve hypomethylation, oxydative stress and environmental factors.(adducts formation). DNA defect is also linked to a more or less delayed apoptotic phenomenon. Exposure to radiations or radiofrequency electromagnetic emissions can also induce DNA alterations into the spermatozoa of infertile men. Although the underlying mechanisms are unclear, these DNA defects have obvious consequences on reproduction of mammalian species. Detection of such anomalies before ART, a...
Presse médicale (Paris, France : 1983), Jan 2, 1996
Management of male infertility has been totally changed with the development of intracytoplasmic ... more Management of male infertility has been totally changed with the development of intracytoplasmic sperm injection (ICSI). This technique, used for in vitro fertilization, consists in injecting a single spermatozoa into the ovum. Intracytoplasmic sperm injection can be proposed in case of severe male sterility (oligoasthenospermia) or after repeated failure of in vitro fertilization. Satisfactory rates of fecondation and pregnancy have been obtained. Results have also been obtained with sperm taken from the epididymis or testis. The risks involved concern the transmission of poorly understood genetic defects leading to the infertility. The rates of reported malformations and chromosome anomalies are quite low. Intracytoplasmic sperm injection can be useful in managing most cases of male infertility which to date have had to rely on donor sperm. Long-term results have not yet been obtained.
Contraception, fertilité, sexualité (1992), 1993
Results of french multiple collaborative studies are reported to evaluate the possible benefits o... more Results of french multiple collaborative studies are reported to evaluate the possible benefits of coculture in human IVF program. Prospective randomized study of IVF with transfer on day two versus IVF with transfer on day 6 shows that coculture does not improve overall results but leads to a reduction of triple or quadruple pregnancies. When applied to patients with more than 4 failures of embryo transfers on day 2, delayed transfer of blastocyst results in a high rate of clinical pregnancy (39%). Transfers of frozen-thawed blastocysts result in 17.8% ongoing pregnancies. Coculture is also a powerful tool for understanding early embryo development: when fertilization arises from sperm with poor characteristics or from frozen sperm of donor, embryo development appears to be altered: in these situations, rate of normally developing embryo is reduced and early embryo development becomes independent of fertilization rate.
Gynécologie Obstétrique & Fertilité, 2001
Physiologie, pathologie et thérapie de la reproduction chez l’humain, 2011
Depuis quelques annees, plusieurs publications ont mis en exergue l’avantage de l’examen du sperm... more Depuis quelques annees, plusieurs publications ont mis en exergue l’avantage de l’examen du sperme au fort grossissement (× 6 600), permettant l’evaluation de la morphologie du noyau spermatique et la densite de sa chromatine en temps reel avant micro-injection ovocytaire. Ce procede est communement appele motile sperm organelle morphology examination (MSOME). La technique d’ICSI est appelee intracytoplasmic morphologically selected sperm injection (IMSI). En reproduction, nous deplorons helas plus d’echecs que de succes. Les causes des echecs sont multiples, d’origine maternelle mais aussi paternelle. En effet, un sperme inadequat peut conduire au pire a un defaut de fecondation ou de developpement embryonnaire precoce et au mieux a une implantation embryonnaire suivie d’une fausse couche.
Male Sterility and Motility Disorders, 1999
Since the conception of in vitro fertilization (IVF), it has been gradually accepted that artific... more Since the conception of in vitro fertilization (IVF), it has been gradually accepted that artificial reproductive technology does not imply a higher risk of congenital malformation.
Reproductive BioMedicine Online, 2009
His clinical practice is focused on endometriosis, reproductive surgery and assisted reproductive... more His clinical practice is focused on endometriosis, reproductive surgery and assisted reproductive technology. His research interest is focused on reproductive toxicology, heavy metals and trace elements.
Maturitas, 2014
Women's fertility potential is declining with age because of multiples intrinsic and extrinsic fa... more Women's fertility potential is declining with age because of multiples intrinsic and extrinsic factors such as life style, oxidative stress and/or endocrine disruptors and is affecting the ability of these women to conceive naturally. This declining fertility potential and the late age of motherhood is increasing significantly the number of patients consulting infertility specialists. Different strategies of investigation and management are proposed to patients over 40 in order to overcome their infertility and improve the live birth rate in these patients. Intra Uterine Insemination (IUI) in women over 40 is associated with a low rate of ongoing pregnancy and IUI should not therefore be offered always as the first line of treatment. When the predictive factors are positive IVF/ICSI seem to be good alternatives until 43 years of age. Customized ovarian stimulation and flexible laboratory methods such as in vitro maturation (IVM), preimplantation genetic diagnosis (PGD), embryo vitrification and transfer after thawing in subsequent natural or artificial cycles can improve the success rate of ART in patients over 40. Meanwhile, oocyte and embryos donation remain good options for patient over 40 with a bad prognosis and can lead to successful ongoing pregnancies until 45 years of age. Ovarian tissue cryopreservation, oocyte vitrification at the germinal vesicle (GV) stage or metaphase II stage present a breakthrough for fertility preservation but the ideal age for starting fertility preservation is still debated as well as the minimum number of oocytes to be vitrified in order to optimize the chances of pregnancy when needed at an older age. This manuscript reports the results of our own experience from patients older than 40 in the light of the published data and discusses the different therapeutic alternatives which can be proposed to patients over 40 consulting ART centres.
Journal of In Vitro Fertilization and Embryo Transfer, 1985
Twenty women, suffering from infertility due to histologically proved genital tuberculosis, were ... more Twenty women, suffering from infertility due to histologically proved genital tuberculosis, were managed by in vitro fertilization (IVF) in 49 attempts. The main histopathological lesions were tuberculous salpingitis in 12 women, tuberculous endometritis in 5, and pelvic peritoneal tuberculosis in 3. A preliminary evaluation including, particularly, a laparoscopy and a hysteroscopy was performed. Five deliveries and one ongoing pregnancy were obtained. The results suggest that IVF nowadays represents the only treatment for tuberculous infertility.
Fertility and Sterility, 1998
Preliminary results of the study have been presented at the 8th World Congress on In Vitro Fertil... more Preliminary results of the study have been presented at the 8th World Congress on In Vitro Fertilization and Alternate Assisted Reproduction, Kyoto, Japan, September 12–15, 1993. ... Received 11 January 1994; received in revised form 22 July 1994; accepted 22 July 1994.
Fertility and Sterility, 2004
Fertility and Sterility, 2003
Objective: It is well known that FSH is the key hormone of the follicular recruitment and growth ... more Objective: It is well known that FSH is the key hormone of the follicular recruitment and growth and that LH has a major role in the late follicular phase. Recombinant LH has recently become available and could be given separately from FSH. This study aims to assess the potential benefit of using rec-hFSH alone in the first part of the follicular phase followed with rec-hLH alone in the late follicular phase to mimic the physiologic cycle. Design: Prospective descriptive study in patients having normal basal hormonal status and undergoing ART. Materials and Methods: Patients received luteal phase GnRH agonist long protocol. After the down regulation confirmation by US, they received rec-hFSH at a starting dose of 100 to 300 IU depending on the patient profile.When at least one follicle with a diameter greater than 14 mm was observed, rec-hFSH was discontinued. The patient was given rec-hLH alone at a fixed daily dose of 150 IU until day hCG. Ovulation was triggered with hCG when the leading follicle reached a size of 18 mm in diameter. Results: Nineteen patients completed the treatment. Demographic characteristics were as follows: mean age of 35.1 Ϯ 3.7, basal FSH of 6.5 Ϯ 1.2 IU/l, basal LH of 5.4 Ϯ 2.1, basal inhibin B of 72 Ϯ 13 ng/ml, and MIS of 1.9 Ϯ 1.4. The mean attempt range was 2.2. Patients received a mean starting dose of 215 IU of rec-hFSH and a mean total dose of rec-hFSH of 1995 Ϯ 530 IU. An average of 283 IU of rec-hLH (Luveris(r)) was given. The mean stimulation duration was 12.0 Ϯ 1.1 days. The mean number of oocytes retrieved was 10.9 Ϯ 6.1 with a percentage of meta II oocytes of 80.4%; 6.3 Ϯ 3.9 embryos were obtained, 2.6 embryos were frozen. The mean number of embryos transferred was 2.4 resulting in a clinical pregnancy rate per OPU of 42% with an implantation rate per embryo transferred of 20.6%. This study is still ongoing. The final results will be presented at ASRM. Conclusion: This new stimulation protocol showed that rec-hLH alone at a daily dose of 150 IU in late follicular phase is able to maintain the follicular development and maturation, without any deleterious effect of clinical pregnancy rate.
Fertility and Sterility, 2003
Genesis, 1995
Peer reviewe
Human Reproduction, 1988
LHRH agonists are being increasingly used in ovulation stimulation protocols in IVF programmes. W... more LHRH agonists are being increasingly used in ovulation stimulation protocols in IVF programmes. We have compared the results of two methods of utilization of LHRH agonists. In the long protocol, gonadotrophin stimulation was only commenced after a preliminary period of pituitary desensitization with LHRH agonist. In the short protocol, exogenous gonadotrophins were administered shortly after the start of LHRH agonist therapy, benefiting from the gonadotrophin flare-up effect. One-hundred-and-eighty-six patients were divided equally between the two treatments. There was no difference in the ovarian response on the day of HCG or the number of mature oocytes recovered. The cleavage rate of mature oocytes was higher in the short protocol (70, versus 56, P less than 0.01). The ongoing pregnancy rate per treatment cycle was similar in both groups (18, in the long protocol and 16, in the short protocol). Analysis of the luteal phases revealed a trend for higher progesterone values in the long protocol although this was only significant on the second day following oocyte retrieval. As the clinical results were similar other factors should be taken into account when deciding therapy. These include patient convenience, cost and side-effects. Other schedules of ovulation stimulation using LHRH agonists are discussed.
The present invention pertains to the use of DNase to treat female infertility and the use of cfD... more The present invention pertains to the use of DNase to treat female infertility and the use of cfDNA as a marker of female infertility.
Gynécologie Obstétrique & Fertilité, 2006
Gynécologie Obstétrique & Fertilité, 2007
Gynécologie, obstétrique & fertilité, 2008
Numerous recent studies involve DNA damages associated with poor fertilization rates, early embry... more Numerous recent studies involve DNA damages associated with poor fertilization rates, early embryo development defect, and poor quality of conceptus following Assisted Reproductive Technologies (ART). The authors denounce a particularly high rate of miscarriages and childhood cancer or dominant genetic mutations such as achondroplasia, Apert syndrome or aberrant gene imprinting such as Angelman and Beckwith Wiedeman syndromes. Gametes DNA defects have numerous origins which are difficult to determine; they are known to involve hypomethylation, oxydative stress and environmental factors.(adducts formation). DNA defect is also linked to a more or less delayed apoptotic phenomenon. Exposure to radiations or radiofrequency electromagnetic emissions can also induce DNA alterations into the spermatozoa of infertile men. Although the underlying mechanisms are unclear, these DNA defects have obvious consequences on reproduction of mammalian species. Detection of such anomalies before ART, a...
Presse médicale (Paris, France : 1983), Jan 2, 1996
Management of male infertility has been totally changed with the development of intracytoplasmic ... more Management of male infertility has been totally changed with the development of intracytoplasmic sperm injection (ICSI). This technique, used for in vitro fertilization, consists in injecting a single spermatozoa into the ovum. Intracytoplasmic sperm injection can be proposed in case of severe male sterility (oligoasthenospermia) or after repeated failure of in vitro fertilization. Satisfactory rates of fecondation and pregnancy have been obtained. Results have also been obtained with sperm taken from the epididymis or testis. The risks involved concern the transmission of poorly understood genetic defects leading to the infertility. The rates of reported malformations and chromosome anomalies are quite low. Intracytoplasmic sperm injection can be useful in managing most cases of male infertility which to date have had to rely on donor sperm. Long-term results have not yet been obtained.
Contraception, fertilité, sexualité (1992), 1993
Results of french multiple collaborative studies are reported to evaluate the possible benefits o... more Results of french multiple collaborative studies are reported to evaluate the possible benefits of coculture in human IVF program. Prospective randomized study of IVF with transfer on day two versus IVF with transfer on day 6 shows that coculture does not improve overall results but leads to a reduction of triple or quadruple pregnancies. When applied to patients with more than 4 failures of embryo transfers on day 2, delayed transfer of blastocyst results in a high rate of clinical pregnancy (39%). Transfers of frozen-thawed blastocysts result in 17.8% ongoing pregnancies. Coculture is also a powerful tool for understanding early embryo development: when fertilization arises from sperm with poor characteristics or from frozen sperm of donor, embryo development appears to be altered: in these situations, rate of normally developing embryo is reduced and early embryo development becomes independent of fertilization rate.
Gynécologie Obstétrique & Fertilité, 2001
Physiologie, pathologie et thérapie de la reproduction chez l’humain, 2011
Depuis quelques annees, plusieurs publications ont mis en exergue l’avantage de l’examen du sperm... more Depuis quelques annees, plusieurs publications ont mis en exergue l’avantage de l’examen du sperme au fort grossissement (× 6 600), permettant l’evaluation de la morphologie du noyau spermatique et la densite de sa chromatine en temps reel avant micro-injection ovocytaire. Ce procede est communement appele motile sperm organelle morphology examination (MSOME). La technique d’ICSI est appelee intracytoplasmic morphologically selected sperm injection (IMSI). En reproduction, nous deplorons helas plus d’echecs que de succes. Les causes des echecs sont multiples, d’origine maternelle mais aussi paternelle. En effet, un sperme inadequat peut conduire au pire a un defaut de fecondation ou de developpement embryonnaire precoce et au mieux a une implantation embryonnaire suivie d’une fausse couche.
Male Sterility and Motility Disorders, 1999
Since the conception of in vitro fertilization (IVF), it has been gradually accepted that artific... more Since the conception of in vitro fertilization (IVF), it has been gradually accepted that artificial reproductive technology does not imply a higher risk of congenital malformation.
Reproductive BioMedicine Online, 2009
His clinical practice is focused on endometriosis, reproductive surgery and assisted reproductive... more His clinical practice is focused on endometriosis, reproductive surgery and assisted reproductive technology. His research interest is focused on reproductive toxicology, heavy metals and trace elements.
Maturitas, 2014
Women's fertility potential is declining with age because of multiples intrinsic and extrinsic fa... more Women's fertility potential is declining with age because of multiples intrinsic and extrinsic factors such as life style, oxidative stress and/or endocrine disruptors and is affecting the ability of these women to conceive naturally. This declining fertility potential and the late age of motherhood is increasing significantly the number of patients consulting infertility specialists. Different strategies of investigation and management are proposed to patients over 40 in order to overcome their infertility and improve the live birth rate in these patients. Intra Uterine Insemination (IUI) in women over 40 is associated with a low rate of ongoing pregnancy and IUI should not therefore be offered always as the first line of treatment. When the predictive factors are positive IVF/ICSI seem to be good alternatives until 43 years of age. Customized ovarian stimulation and flexible laboratory methods such as in vitro maturation (IVM), preimplantation genetic diagnosis (PGD), embryo vitrification and transfer after thawing in subsequent natural or artificial cycles can improve the success rate of ART in patients over 40. Meanwhile, oocyte and embryos donation remain good options for patient over 40 with a bad prognosis and can lead to successful ongoing pregnancies until 45 years of age. Ovarian tissue cryopreservation, oocyte vitrification at the germinal vesicle (GV) stage or metaphase II stage present a breakthrough for fertility preservation but the ideal age for starting fertility preservation is still debated as well as the minimum number of oocytes to be vitrified in order to optimize the chances of pregnancy when needed at an older age. This manuscript reports the results of our own experience from patients older than 40 in the light of the published data and discusses the different therapeutic alternatives which can be proposed to patients over 40 consulting ART centres.
Journal of In Vitro Fertilization and Embryo Transfer, 1985
Twenty women, suffering from infertility due to histologically proved genital tuberculosis, were ... more Twenty women, suffering from infertility due to histologically proved genital tuberculosis, were managed by in vitro fertilization (IVF) in 49 attempts. The main histopathological lesions were tuberculous salpingitis in 12 women, tuberculous endometritis in 5, and pelvic peritoneal tuberculosis in 3. A preliminary evaluation including, particularly, a laparoscopy and a hysteroscopy was performed. Five deliveries and one ongoing pregnancy were obtained. The results suggest that IVF nowadays represents the only treatment for tuberculous infertility.
Fertility and Sterility, 1998
Preliminary results of the study have been presented at the 8th World Congress on In Vitro Fertil... more Preliminary results of the study have been presented at the 8th World Congress on In Vitro Fertilization and Alternate Assisted Reproduction, Kyoto, Japan, September 12–15, 1993. ... Received 11 January 1994; received in revised form 22 July 1994; accepted 22 July 1994.
Fertility and Sterility, 2004
Fertility and Sterility, 2003
Objective: It is well known that FSH is the key hormone of the follicular recruitment and growth ... more Objective: It is well known that FSH is the key hormone of the follicular recruitment and growth and that LH has a major role in the late follicular phase. Recombinant LH has recently become available and could be given separately from FSH. This study aims to assess the potential benefit of using rec-hFSH alone in the first part of the follicular phase followed with rec-hLH alone in the late follicular phase to mimic the physiologic cycle. Design: Prospective descriptive study in patients having normal basal hormonal status and undergoing ART. Materials and Methods: Patients received luteal phase GnRH agonist long protocol. After the down regulation confirmation by US, they received rec-hFSH at a starting dose of 100 to 300 IU depending on the patient profile.When at least one follicle with a diameter greater than 14 mm was observed, rec-hFSH was discontinued. The patient was given rec-hLH alone at a fixed daily dose of 150 IU until day hCG. Ovulation was triggered with hCG when the leading follicle reached a size of 18 mm in diameter. Results: Nineteen patients completed the treatment. Demographic characteristics were as follows: mean age of 35.1 Ϯ 3.7, basal FSH of 6.5 Ϯ 1.2 IU/l, basal LH of 5.4 Ϯ 2.1, basal inhibin B of 72 Ϯ 13 ng/ml, and MIS of 1.9 Ϯ 1.4. The mean attempt range was 2.2. Patients received a mean starting dose of 215 IU of rec-hFSH and a mean total dose of rec-hFSH of 1995 Ϯ 530 IU. An average of 283 IU of rec-hLH (Luveris(r)) was given. The mean stimulation duration was 12.0 Ϯ 1.1 days. The mean number of oocytes retrieved was 10.9 Ϯ 6.1 with a percentage of meta II oocytes of 80.4%; 6.3 Ϯ 3.9 embryos were obtained, 2.6 embryos were frozen. The mean number of embryos transferred was 2.4 resulting in a clinical pregnancy rate per OPU of 42% with an implantation rate per embryo transferred of 20.6%. This study is still ongoing. The final results will be presented at ASRM. Conclusion: This new stimulation protocol showed that rec-hLH alone at a daily dose of 150 IU in late follicular phase is able to maintain the follicular development and maturation, without any deleterious effect of clinical pregnancy rate.
Fertility and Sterility, 2003