Andrea von Groll - Academia.edu (original) (raw)
Papers by Andrea von Groll
Vittalle Revista De Ciencias Da Saude, Aug 7, 2009
As micobactérias não-tuberculosas são espécies ambientais com crescente relevância clínica, por s... more As micobactérias não-tuberculosas são espécies ambientais com crescente relevância clínica, por sua relação com diversos tipos de infecções. Geralmente, apresentam maior resistência intrínseca aos antimicobacterianos convencionais, impulsionando assim a busca de novas alternativas farmacológicas, como as obtidas de fontes naturais. Este estudo avaliou a atividade de clássicos antituberculosos, como estreptomicina (SMR), etambutol (EMB), isoniazida (INH) e rifampicina (RIF), bem como extratos vegetais oriundos da Mata Atlântica (Brasil) frente ao Mycobacterium fortuitum e Mycobacterium malmoense. Foram observadas as seguintes concentrações mínimas inibitórias (CMI) para o M. fortuitum: EMB = 16µg/mL; SMR = 4µg/mL; INH e RIF 1µg/mL; extrato bruto Plathymenia foliolosa = 200µg/mL. Já para o M. malmoense, as CMI encontradas foram: SMR = 4µg/mL; INH = 1µg/mL e RIF = 0,25µg/mL. O EMB não inibiu o crescimento bacteriano desta espécie. Os compostos naturais ativos contra o M. malmoense foram os extratos Siparuna arianeae, Cedrela fissilis e Peschiera affinis, que apresentaram CMI de 200µg/mL, enquanto a CMI de Ficus gomelleria foi de 100µg/mL.
Vittalle Revista De Ci Ncias Da Sa De, Aug 7, 2009
Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do triclo... more Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do triclosan é uma enoil-redutase que está presente no Mycobacterium tuberculosis, sendo também o principal alvo da isoniazida (INH), um importante fármaco utilizado para o tratamento da tuberculose. O uso de biocidas que compartilham alvos similares com antimicrobianos de uso humano podem gerar resistência cruzada com estes e devem ter o seu uso criteriosamente avaliado. Neste estudo, determinou-se a concentração mínima inibitória (CMI) do triclosan e INH para 46 isolados clínicos de M. tuberculosis previamente identificados como resistentes a INH, tendo sido observados altos níveis de resistência para INH e sensibilidade ao triclosan para todas as cepas.
Vittalle Revista De Ciencias Da Saude, Jan 11, 2011
A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de ... more A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de casos. Considerando o aumento no número de casos por cepas multidrogarresistente (MDR-TB) e o reduzido arsenal terapêutico, faz-se necessária à busca de novas alternativas farmacológicas para o tratamento de TB. Os extratos vegetais tem sido uma fonte importante para o desenvolvimento de substâncias com atividade antimicrobiana, principalmente, em países ricos em biodiversidade, como é o caso do Brasil. Outra estrategia é a síntese de novas moléculas que podem ser desenhadas a partir de estruturas já conhecidas ou buscando atingir novos alvos. O grupo de pesquisa do Laboratório de Micobactérias/Biologia Molecular da FURG tem avaliado a atividade antibacteriana de extratos vegetais e moléculas sintéticas buscando novas alternativas terapêuticas para TB.
Revista Argentina de Microbiología, 2015
Asymptomatic meningococcus carriers in hospitals is a risk factor for acquiring meningococcal dis... more Asymptomatic meningococcus carriers in hospitals is a risk factor for acquiring meningococcal disease. Meningococcal carrier (MC) frequency was investigated in oropharyngeal swab samples collected from 200 staff members at a teaching hospital from Brazil. MC prevalence was 9% (95% CI 5-13%). Risk factors associated with MC were: mean age of 26.5 years, male gender, bar attendance frequency and number of persons/house. Of 18 isolated meningococcal strains, 14 were non-groupable (NG), 3 corrresponded to serogroup B and 1 to serogroup 29E. The frequency of serotypes and serosubtypes was heterogenous, with a slight predominance of serotypes 4 and 7 and serosubtypes P1.7 and P1.5. Most strains (n=13) were susceptible to the antimicrobials tested. The ctrA gene (PCR) was identified in 9 (64.3%) of the 14 NG strains, suggesting virulence in most of the NG isolated strains. Therefore, a constant surveillance of these asymptomatic carriers is required.
The International Journal of Tuberculosis and Lung Disease
To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent c... more To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST).
![Research paper thumbnail of The laboratory as a tool to qualify tuberculosis diagnosis [Short Communication]](https://attachments.academia-assets.com/43177314/thumbnails/1.jpg)
](The International Journal of Tuberculosis and Lung Disease
To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent c... more To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST).
Lecture Notes in Computer Science, 2014
ABSTRACT
Lecture Notes in Computer Science, 2014
Efficient transfection of eukaryotic cells is an essential step of optimizing gene expression for... more Efficient transfection of eukaryotic cells is an essential step of optimizing gene expression for genetic therapy and for stimulating the immune response induced by the DNA vaccination. The DNA topology and the vehicle used to deliver it are the two aspects explored in this work. A plasmid expressing the ?-galactosidase enzyme was used to transfect Vero cells in order to evaluate liposome-mediated transfection of circular and linear DNA. The results showed a low efficiency of linear DNA:liposome complexes in transfecting the cells, probably due to an impaired association between the two components. Atomic force microscopy has confirmed the difference in the complex size: circular topology leads to larger complexes than the linear one. Based on an analytic theory, low concentrations of amphiphilic molecules were used to neutralize the linearized plasmid. We were able to obtain an increased transgene expression without the toxicity observed with the usual linear DNA liposome delivery ...
Biotechnology Progress, 2006
A plasmid expressing the -galactosidase enzyme was used to transfect Vero cells in order to evalu... more A plasmid expressing the -galactosidase enzyme was used to transfect Vero cells in order to evaluate the efficiency of a liposome-mediated transfection by circular and linear DNA. The results obtained showed a low rate of transfection by linear DNA:liposome complexes. To explore whether the structure of the complexes was interfering with the transfection, atomic force microscopy (AFM) was used. It has confirmed the difference between the linear and circular condensates: whereas the circular DNA:liposome complexes presented compact spherical or cylindrical structures of about 100-800 nm, the linear DNA showed pearl necklace-like structures, with pearls varying from 250 to 400 nm. On the basis of the theory proposed by , low concentrations of cationic amphihile were used to neutralize or reverse the DNA charge in order to improve the transfection efficiency of the linear DNA. Using this method, we were able to obtain the expression of the transgene without an associated toxicity observed with the linear DNA liposome delivery.
gene
RESUMO Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do... more RESUMO Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do triclosan é uma enoil-redutase que está presente no Mycobacterium tuberculosis, sendo também o principal alvo da isoniazida (INH), um importante fármaco ...
Anais da Academia Brasileira de Ciências, 2006
Different human immunodeficiency virus type 1 (HIV-1) subtypes may have distinct biological, immu... more Different human immunodeficiency virus type 1 (HIV-1) subtypes may have distinct biological, immunological and pathogenic properties. Efficiency of mother-to-child transmission (MTCT) may be among those properties, but few and controversial results have been described so far. In this study, 102 children born from HIV-1-infected mothers between 1998 and 2004 in the city of Rio Grande, Brazil were analyzed for potential risk factors associated with MTCT. That geographic region is characterized by a high proportion of subtype C-infected subjects, and it allowed comparison between subtypes B and C and their influence on MTCT. The analysis also included clinical, obstetric and immunological parameters. Multivariate regression analyses were conducted to evaluate the influence of the parameters on MTCT, and prevalence ratios (PR) and 95% confidence intervals (CI95) were also calculated. A surprisingly high prevalence of subtype C of over 70% was found. Only the HIV viral load and the use of ACTG 076 protocol were predictive of MTCT. HIV subtype and CD4 T-cell counts were not associated with increased risk of transmission. Although a clear expansion of subtype C is evident in southern Brazil, it does not seem to correlate with increased risk of vertical transmission.
European Respiratory Journal, Sep 1, 2012
Number: 93 Publication Number: 3279 Abstract Group: 7.2. Paediatric Asthma and Allergy Keyword 1:... more Number: 93 Publication Number: 3279 Abstract Group: 7.2. Paediatric Asthma and Allergy Keyword 1: Asthma -management Keyword 2: Anti-inflammatory Keyword 3: Bacteria Title: Inhaled corticosteroids and risk of oropharyngeal colonization by streptococcus pneumoniae in children with asthma Dr. Linjie 466 Zhang zhanglinjie63@yahoo.com.br MD 1 , Dr. Sílvio 467 Prietsch Body: Introduction: recent epidemiological studies have raised concerns about possible link between use of inhaled corticosteroids (ICS) and risk of pneumonia in patients with chronic obstructive pulmonary disease.
Fems Immunol Med Microbiol, 2010
RD Rio is a novel Mycobacterium tuberculosis lineage of the Latin American-Mediterranean (LAM) fa... more RD Rio is a novel Mycobacterium tuberculosis lineage of the Latin American-Mediterranean (LAM) family. LAM has been found worldwide but is more predominant in South America. The aim of this study was to assess the presence of the RD Rio lineage and LAM family in the city of Rio Grande, Brazil, and to investigate the fitness of these strains based on determination of their growth rate. Fifty clinical isolates of M. tuberculosis were genotyped and 43 different patterns were found by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats. The predominant genotypes belonged to the LAM family (54% of the strains) followed by clade T (22%) and Haarlem (16%). The RD Rio lineage represented 38% of the total strains and 70.4% of the LAM strains found in this study. Strains belonging to the LAM family showed a fitness advantage when comparing their rate of growth with that of non-LAM strains, but a significant difference between RD Rio and non-RD Rio strains was not confirmed. Ã Significant difference of the rate of growth (P o 0.05) by t-test.
Revista de Epidemiologia e Controle de Infecção, 2016
Revista de Epidemiologia e Controle de Infecção, 2014
Biomedica : revista del Instituto Nacional de Salud, 2015
Reports of Mycobacterium tuberculosis resistant to multiple drugs are increasing globally and lab... more Reports of Mycobacterium tuberculosis resistant to multiple drugs are increasing globally and laboratories are becoming increasingly aware of the need for drug susceptibility testing. In recent years, due to the long time required by conventional drug susceptibility testing, new approaches have been proposed for faster detection of drug resistance, such as the nitrate reductase assay, considered fast and inexpensive, making it a good diagnostic tool for low resource countries. The present study proposed a fast direct colorimetric drug susceptibility testing method in a microplate format using solid medium. The diagnostic accuracy was evaluated by comparing the proportion method with the direct nitrate reductase assay in plates. Frozen sputum samples, known to be positive, were decontaminated and processed by Petroff method. The decontaminated suspension was used to perform direct nitrate reductase assay in 7H11 medium using 1 µ g/ml rifampicin (RIF), 0.2 µ g/ml isoniazid (INH), 2 µ ...
The Journal of Infection in Developing Countries, 2015
Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carc... more Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases. Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR. It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B. No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found. We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.
Background / Purpose: To investigate the relationship between fitness and drug resistance. Main c... more Background / Purpose: To investigate the relationship between fitness and drug resistance. Main conclusion: Heterogeneity in fitness is a function of the drug resistance mutations and the strain genetic background.
Vittalle Revista De Ciencias Da Saude, Aug 7, 2009
As micobactérias não-tuberculosas são espécies ambientais com crescente relevância clínica, por s... more As micobactérias não-tuberculosas são espécies ambientais com crescente relevância clínica, por sua relação com diversos tipos de infecções. Geralmente, apresentam maior resistência intrínseca aos antimicobacterianos convencionais, impulsionando assim a busca de novas alternativas farmacológicas, como as obtidas de fontes naturais. Este estudo avaliou a atividade de clássicos antituberculosos, como estreptomicina (SMR), etambutol (EMB), isoniazida (INH) e rifampicina (RIF), bem como extratos vegetais oriundos da Mata Atlântica (Brasil) frente ao Mycobacterium fortuitum e Mycobacterium malmoense. Foram observadas as seguintes concentrações mínimas inibitórias (CMI) para o M. fortuitum: EMB = 16µg/mL; SMR = 4µg/mL; INH e RIF 1µg/mL; extrato bruto Plathymenia foliolosa = 200µg/mL. Já para o M. malmoense, as CMI encontradas foram: SMR = 4µg/mL; INH = 1µg/mL e RIF = 0,25µg/mL. O EMB não inibiu o crescimento bacteriano desta espécie. Os compostos naturais ativos contra o M. malmoense foram os extratos Siparuna arianeae, Cedrela fissilis e Peschiera affinis, que apresentaram CMI de 200µg/mL, enquanto a CMI de Ficus gomelleria foi de 100µg/mL.
Vittalle Revista De Ci Ncias Da Sa De, Aug 7, 2009
Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do triclo... more Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do triclosan é uma enoil-redutase que está presente no Mycobacterium tuberculosis, sendo também o principal alvo da isoniazida (INH), um importante fármaco utilizado para o tratamento da tuberculose. O uso de biocidas que compartilham alvos similares com antimicrobianos de uso humano podem gerar resistência cruzada com estes e devem ter o seu uso criteriosamente avaliado. Neste estudo, determinou-se a concentração mínima inibitória (CMI) do triclosan e INH para 46 isolados clínicos de M. tuberculosis previamente identificados como resistentes a INH, tendo sido observados altos níveis de resistência para INH e sensibilidade ao triclosan para todas as cepas.
Vittalle Revista De Ciencias Da Saude, Jan 11, 2011
A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de ... more A tuberculose (TB) é uma doença infecciosa com uma incidência mundial estimada de 8,8 milhões de casos. Considerando o aumento no número de casos por cepas multidrogarresistente (MDR-TB) e o reduzido arsenal terapêutico, faz-se necessária à busca de novas alternativas farmacológicas para o tratamento de TB. Os extratos vegetais tem sido uma fonte importante para o desenvolvimento de substâncias com atividade antimicrobiana, principalmente, em países ricos em biodiversidade, como é o caso do Brasil. Outra estrategia é a síntese de novas moléculas que podem ser desenhadas a partir de estruturas já conhecidas ou buscando atingir novos alvos. O grupo de pesquisa do Laboratório de Micobactérias/Biologia Molecular da FURG tem avaliado a atividade antibacteriana de extratos vegetais e moléculas sintéticas buscando novas alternativas terapêuticas para TB.
Revista Argentina de Microbiología, 2015
Asymptomatic meningococcus carriers in hospitals is a risk factor for acquiring meningococcal dis... more Asymptomatic meningococcus carriers in hospitals is a risk factor for acquiring meningococcal disease. Meningococcal carrier (MC) frequency was investigated in oropharyngeal swab samples collected from 200 staff members at a teaching hospital from Brazil. MC prevalence was 9% (95% CI 5-13%). Risk factors associated with MC were: mean age of 26.5 years, male gender, bar attendance frequency and number of persons/house. Of 18 isolated meningococcal strains, 14 were non-groupable (NG), 3 corrresponded to serogroup B and 1 to serogroup 29E. The frequency of serotypes and serosubtypes was heterogenous, with a slight predominance of serotypes 4 and 7 and serosubtypes P1.7 and P1.5. Most strains (n=13) were susceptible to the antimicrobials tested. The ctrA gene (PCR) was identified in 9 (64.3%) of the 14 NG strains, suggesting virulence in most of the NG isolated strains. Therefore, a constant surveillance of these asymptomatic carriers is required.
The International Journal of Tuberculosis and Lung Disease
To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent c... more To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST).
The International Journal of Tuberculosis and Lung Disease
To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent c... more To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST).
Lecture Notes in Computer Science, 2014
ABSTRACT
Lecture Notes in Computer Science, 2014
Efficient transfection of eukaryotic cells is an essential step of optimizing gene expression for... more Efficient transfection of eukaryotic cells is an essential step of optimizing gene expression for genetic therapy and for stimulating the immune response induced by the DNA vaccination. The DNA topology and the vehicle used to deliver it are the two aspects explored in this work. A plasmid expressing the ?-galactosidase enzyme was used to transfect Vero cells in order to evaluate liposome-mediated transfection of circular and linear DNA. The results showed a low efficiency of linear DNA:liposome complexes in transfecting the cells, probably due to an impaired association between the two components. Atomic force microscopy has confirmed the difference in the complex size: circular topology leads to larger complexes than the linear one. Based on an analytic theory, low concentrations of amphiphilic molecules were used to neutralize the linearized plasmid. We were able to obtain an increased transgene expression without the toxicity observed with the usual linear DNA liposome delivery ...
Biotechnology Progress, 2006
A plasmid expressing the -galactosidase enzyme was used to transfect Vero cells in order to evalu... more A plasmid expressing the -galactosidase enzyme was used to transfect Vero cells in order to evaluate the efficiency of a liposome-mediated transfection by circular and linear DNA. The results obtained showed a low rate of transfection by linear DNA:liposome complexes. To explore whether the structure of the complexes was interfering with the transfection, atomic force microscopy (AFM) was used. It has confirmed the difference between the linear and circular condensates: whereas the circular DNA:liposome complexes presented compact spherical or cylindrical structures of about 100-800 nm, the linear DNA showed pearl necklace-like structures, with pearls varying from 250 to 400 nm. On the basis of the theory proposed by , low concentrations of cationic amphihile were used to neutralize or reverse the DNA charge in order to improve the transfection efficiency of the linear DNA. Using this method, we were able to obtain the expression of the transgene without an associated toxicity observed with the linear DNA liposome delivery.
gene
RESUMO Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do... more RESUMO Triclosan é um biocida amplamente utilizado na comunidade e nos hospitais. Um dos alvos do triclosan é uma enoil-redutase que está presente no Mycobacterium tuberculosis, sendo também o principal alvo da isoniazida (INH), um importante fármaco ...
Anais da Academia Brasileira de Ciências, 2006
Different human immunodeficiency virus type 1 (HIV-1) subtypes may have distinct biological, immu... more Different human immunodeficiency virus type 1 (HIV-1) subtypes may have distinct biological, immunological and pathogenic properties. Efficiency of mother-to-child transmission (MTCT) may be among those properties, but few and controversial results have been described so far. In this study, 102 children born from HIV-1-infected mothers between 1998 and 2004 in the city of Rio Grande, Brazil were analyzed for potential risk factors associated with MTCT. That geographic region is characterized by a high proportion of subtype C-infected subjects, and it allowed comparison between subtypes B and C and their influence on MTCT. The analysis also included clinical, obstetric and immunological parameters. Multivariate regression analyses were conducted to evaluate the influence of the parameters on MTCT, and prevalence ratios (PR) and 95% confidence intervals (CI95) were also calculated. A surprisingly high prevalence of subtype C of over 70% was found. Only the HIV viral load and the use of ACTG 076 protocol were predictive of MTCT. HIV subtype and CD4 T-cell counts were not associated with increased risk of transmission. Although a clear expansion of subtype C is evident in southern Brazil, it does not seem to correlate with increased risk of vertical transmission.
European Respiratory Journal, Sep 1, 2012
Number: 93 Publication Number: 3279 Abstract Group: 7.2. Paediatric Asthma and Allergy Keyword 1:... more Number: 93 Publication Number: 3279 Abstract Group: 7.2. Paediatric Asthma and Allergy Keyword 1: Asthma -management Keyword 2: Anti-inflammatory Keyword 3: Bacteria Title: Inhaled corticosteroids and risk of oropharyngeal colonization by streptococcus pneumoniae in children with asthma Dr. Linjie 466 Zhang zhanglinjie63@yahoo.com.br MD 1 , Dr. Sílvio 467 Prietsch Body: Introduction: recent epidemiological studies have raised concerns about possible link between use of inhaled corticosteroids (ICS) and risk of pneumonia in patients with chronic obstructive pulmonary disease.
Fems Immunol Med Microbiol, 2010
RD Rio is a novel Mycobacterium tuberculosis lineage of the Latin American-Mediterranean (LAM) fa... more RD Rio is a novel Mycobacterium tuberculosis lineage of the Latin American-Mediterranean (LAM) family. LAM has been found worldwide but is more predominant in South America. The aim of this study was to assess the presence of the RD Rio lineage and LAM family in the city of Rio Grande, Brazil, and to investigate the fitness of these strains based on determination of their growth rate. Fifty clinical isolates of M. tuberculosis were genotyped and 43 different patterns were found by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats. The predominant genotypes belonged to the LAM family (54% of the strains) followed by clade T (22%) and Haarlem (16%). The RD Rio lineage represented 38% of the total strains and 70.4% of the LAM strains found in this study. Strains belonging to the LAM family showed a fitness advantage when comparing their rate of growth with that of non-LAM strains, but a significant difference between RD Rio and non-RD Rio strains was not confirmed. Ã Significant difference of the rate of growth (P o 0.05) by t-test.
Revista de Epidemiologia e Controle de Infecção, 2016
Revista de Epidemiologia e Controle de Infecção, 2014
Biomedica : revista del Instituto Nacional de Salud, 2015
Reports of Mycobacterium tuberculosis resistant to multiple drugs are increasing globally and lab... more Reports of Mycobacterium tuberculosis resistant to multiple drugs are increasing globally and laboratories are becoming increasingly aware of the need for drug susceptibility testing. In recent years, due to the long time required by conventional drug susceptibility testing, new approaches have been proposed for faster detection of drug resistance, such as the nitrate reductase assay, considered fast and inexpensive, making it a good diagnostic tool for low resource countries. The present study proposed a fast direct colorimetric drug susceptibility testing method in a microplate format using solid medium. The diagnostic accuracy was evaluated by comparing the proportion method with the direct nitrate reductase assay in plates. Frozen sputum samples, known to be positive, were decontaminated and processed by Petroff method. The decontaminated suspension was used to perform direct nitrate reductase assay in 7H11 medium using 1 µ g/ml rifampicin (RIF), 0.2 µ g/ml isoniazid (INH), 2 µ ...
The Journal of Infection in Developing Countries, 2015
Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carc... more Helicobacter pylori infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the IL-1B and IL-1RN genes and their associations with H. pylori infection, cagA gene of H. pylori, and gastroduodenal diseases. Gastric biopsy samples from 151 patients infected with H. pylori and 76 uninfected individuals were analyzed. H. pylori infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the IL-1B gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the IL-1RN gene was performed by PCR. It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with H. pylori infection and with an increased risk of gastritis in H. pylori-positive patients. Additionally, strains from patients H. pylori-positive carrying the cagA gene was significantly related with the T/T genotype at position -511 of IL-1B. No association of polymorphisms at position +3954 of IL-1B and in the IL-1RN with H. pylori infection and with risk of severe gastric diseases was found. We demonstrated that polymorphisms in the promoter region of the IL-1B gene (at positions -511 and -31) are associated with an enhanced risk of H. pylori infection as well as gastritis in H. pylori-positive patients.
Background / Purpose: To investigate the relationship between fitness and drug resistance. Main c... more Background / Purpose: To investigate the relationship between fitness and drug resistance. Main conclusion: Heterogeneity in fitness is a function of the drug resistance mutations and the strain genetic background.