Andriani Rafael - Academia.edu (original) (raw)
Papers by Andriani Rafael
Jurnal Pembelajaran dan Biologi Nukleus (JPBN), Nov 29, 2023
Marine biota has many benefits for human life. Sponges are a species of marine biota that can be ... more Marine biota has many benefits for human life. Sponges are a species of marine biota that can be used as a producer of antimicrobial compounds. The bacteria found in sponges have an important role in the continuity of life in the sea. The symbiotic lifestyle that occurs in bacteria and sponges has the opportunity to form substitutions for the content of secondary metabolites and enzymes, especially extracellular enzymes (amylase, protease, cellulose and lipase). This study aims to determine how to isolate sponge symbiotic bacteria and identify spongy symbiotic bacteria. The method used is purposive to take sponges. Characterization of bacteria was carried out based on morphology and gram staining. Enzymatic bacterial selection was carried out by testing the activity of the amylase enzyme (soluble starch), lipase enzyme (Teen 80), protease enzyme (skim milk) and cellulose enzyme (carboxyl methyl cellulose). Data analysis was performed in a qualitative descriptive manner by measuring the clear zone in the extracellular enzyme test results. Meanwhile, other research parameters measured in this study were temperature, salinity and pH. The results showed that out of 47 isolated bacteria and 33 of them had extracellular enzymes with 10 bacteria had amylase enzymes, 27 bacteria had lipase enzymes, 2 bacteria had protease enzymes and 1 isolate had cellulose enzymes
Jurnal Penelitian Kehutanan Wallacea, Dec 16, 2023
The Taplel waterfall area which is in the Sisimeni Sanam Education and Training Forest area is on... more The Taplel waterfall area which is in the Sisimeni Sanam Education and Training Forest area is one of the conservation areas in East Nusa Tenggara which has quite high biodiversity, including plants that produce natural colors. However, information on the species diversity of dye plants in the Taplel waterfall area is still lacking. The objective of this study was to uncover the diversity of natural dye plants in the Taplel waterfall area, Sisimeni Sanam Training Forest, Takari District, Kupang Regency. Data collection was carried out by setting sample plots measuring 2 x 2 meters (seedlings), 5 x 5 meters (saplings), 10 x 10 meters (poles), and 20 x 20 meters (trees). Analysis of the importance of species using the Importance Value Index, and species diversity using the Species Diversity Index (Shannon-Wiener Index). Species of natural dye plants found in the
Jambura Edu Biosfer Journal, May 22, 2020
Today's society is vulnerable to various diseases. Everyone needs to be given understanding in ch... more Today's society is vulnerable to various diseases. Everyone needs to be given understanding in choosing healthy foods for the body. β-carotene is one of the good compounds contained in vegetables and fruits. This research was conducted to determine the content of β-carotene compounds in fruit (Arenga pinata). Samples were obtained from Baumata village. This research uses thin layer chromatography (TLC) and spectrophotometry methods. The research data were analyzed based on linear regression equations and relevant reference studies. The results of the study using the TLC method showed that the Rf value for palm fruit was 0.943. Spectrophotometric examination showed the content of β-carotene compounds from palm fruit extract at a wavelength of 470 nm had a concentration of 26.18 ppm. Based on the results of these studies it can be concluded that the palm fruit contains β-carotene. Thus palm fruit can be used as one of the choices of healthy food for the community and also as a choice of sources of vitamin A.
Indigenous Biologi, Aug 18, 2021
Palm oil (Arenga pinnata Merr.) is a plantation plant that has the potential to be cultivated in ... more Palm oil (Arenga pinnata Merr.) is a plantation plant that has the potential to be cultivated in the future, because it has high economic value and is prospective for commercial cultivation considering its various uses. Enau is a plant that has been developed from generation to generation on the island of Timor. The community in Nekmese Village manages enau still in the traditional way. Vitamin C or ascorbic acid is one of the vitamins made from hexose derivatives that are soluble in water and easily oxidized. The purpose of this study was to determine the content of vitamin C in the flesh of palm fruit. This research was carried out in May 2019 at the Kupang State Agricultural Polytechnic TPH Laboratory. Determination of the vitamin C content in the sample was determined by titration using an iodine solution. The content of vitamin C in palm fruit flesh based on the temperature treatment of 5°C 10°C and 15°C showed that storage treatment at 5°C had the highest vitamin C content with an average of 4.14 mg followed by storage at 10°C with an average of 3.33 mg and storage at a temperature of 15°C with an average vitamin C content of 3.02 mg. This study also showed that the longer the palm fruit is stored, the lower the vitamin C content in the palm fruit.
Aacl Bioflux, 2019
The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natura... more The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natural forests and reforestation sites were compared in three areas from Philippines: Central Visayas-Bais, Negros Oriental, Alcantara, Cebu and Pangangan Island, Bohol. Three diseases were identified, namely the Brown Leaf Spot disease (BRS), Black Leaf Spot disease (BLS) and White Leaf Spot disease (WLS). BRS was found in Sonneratia alba and Avicennia marina in natural forests and reforestation sites in all areas, while BLS and WLS were found only in Bais, with BLS occurring in Ceriops decandra and Osbornia octodonta in natural forests and in Rhizophora stylosa in reforested sites. WLS occurred only in A. marina in natural forests. Disease prevalence for BRS was significantly higher in reforested areas (F=8.477; p=0.001). 4 fungi genera were consistently found in infected leaves: Aspergillus, Penicillium, Pseudocercosporella and Rhizopus. The occurrence percentage was higher in natural forests than in reforestation sites for all fungi.
Indigenous Biologi, Mar 2, 2021
Tumbuhan mangrove merupakan salah satu ekosistem pantai yang mendominasi wilayah pantai suatu dae... more Tumbuhan mangrove merupakan salah satu ekosistem pantai yang mendominasi wilayah pantai suatu daerah dan berpotensi untuk dikembangkan dalam penanggulangan berbagai penyakit baik infeksi pada ikan budidaya maupun sebagai medi pengobatan alami bagi manusia, karena senyawa metaboit sekunder yang terkandung didalamnya mampu berkhasiat sebagai antibakteri. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak kasar kulit batang mangrove A. marina terhadap pertumbuhan bakteri E. coli dan konsentrasi optimum dari estrak kasar kulit batang mangrove A. marina dalam menghambat pertumbuhan bakteri E. coli. Metode yang digunakan dalam penelitian ini metode eksperimen laboratorium dengan 4 perlakuan yaitu konsentrasi 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL dan 1 kontrol tanpa pemberian ekstrak dengan 3 kali ulangan. Hasil pengamatan diameter zona hambat dianalisis secara statistik menggunakan analisis variansi (ANOVA) satu jalur dengan signifikansi 5%. Analisis statistik dilakukan menggunakan program SPSS versi 16.0. Hasil penelitian menunjukkan bahwa ekstrak kasar kulit batang mangrove A. marina berpengaruh terhadap pertumbuhan bakteri E. coli. Hal ini dapat dilihat dar konsentrasi yang digunakan mulai dari 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL memiliki rata-rata zona hambat yang berkisar antara 26 mm, 27.33 mm, 27.66 mm dan 28.66 mm. Dengan demikian semakin tinggi konsentrasi ekstrak yang digunakan, maka semakin besar daya hambat yang terbentuk.
Bioedukasi : Jurnal Pendidikan Biologi FKIP UM Metro, May 31, 2022
Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a... more Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a jackfruit crackers and other food product. Jackfruit seed consist of carbohydrate (36,7 g/100 g) and also as a source minerals. The availability of jackfruit seed was found abundance but the use of it not maximize yet. The flour from jackfruit seed can be used an alternative for making food product. The flour extracted from (40,891) jackfruit seed have longer storage time and easy to turn it to other product.
Indigenous Biologi, Aug 18, 2021
Labu kuning (Cucurbita moschata (Duch.) Poir) merupakan salah satu sayuran yang kaya akan βkarote... more Labu kuning (Cucurbita moschata (Duch.) Poir) merupakan salah satu sayuran yang kaya akan βkaroten sebagai prekursor vitamin A dan memiliki kandungan karbohidrat yang cukup tinggi. Pengolahan labu kuning dapat dilakukan untuk menghasilkan berbagai macam produk salah satunya cake. Cake merupakan adonan panggang dan adonan liquid yang terbuat dari tepung terigu, gula, garam, bahan pengembang, shortening, susu, telur, dan penambah aroma. Penelitian ini bertujuan untuk mengetahui kadar β-karoten yang terdapat pada cake labu kuning dan untuk mengetahui daya terima β-karoten pada cake labu kuning. Penelitian ini merupakan penelitian eksperimen. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 1 kontrol 4 perlakuan dan masing-masing perlakuan diulang sebanyak 3 kali, selain β-Karoten yang diuji 2 kali. Tahap penelitian terdiri dari pembuatan tepung dan pembuatan cake. Metode pengumpulan data meliputi uji organoleptik dan uji β-karoten. Data dianalisis menggunakan analisa varian/ANOVA untuk mengetahui perbedaan kualitas, dan apabila terdapat pengaruh maka dilanjutkan dengan uji Tukey. Hasil penelitian uji organoleptik menunjukan bahwa pada parameter warna tidak memberikan pengaruh yang nyata, sehingga tidak dilanjutkan dengan uji Tukey, dari segi aroma yaitu A1 merupakan perlakuan terbaik dengan nilai 3,56, dari segi tekstur perlakuan terbaik adalah A1 dengan nilai 4,04 dan dari segi rasa perlakuan terbaik adalah A1 dengan nilai 3,80. Untuk uji β-karoten, perlakuan cake labu kuning dengan kadar β-karoten tertinggi terdapat pada A4 dengan rata-rata 0,262 dan kadar terendah terdapat pada A0 dengan rata-rata 0,018. Berdasarkan data hasil penelitian, tepung labu kuning layak dijadikan salah satu sumber tepung alternatif karena cake yang dihasilkan memenuhi standar mutu SNI baik dari segi warna, rasa, aroma, tekstur maupun kadar β-karoten.
Acta Aquatica, Mar 25, 2021
Mangrove didefinisikan sebagai pohon dan semak yang memiliki kemanpuan untuk tumbuh di pesisir pa... more Mangrove didefinisikan sebagai pohon dan semak yang memiliki kemanpuan untuk tumbuh di pesisir pantai. Mangrove tumbuh di lingkungan dengan kondisi lingkungan ekstrim seperti daerah yang berlumpur mendapat hempasan ombak pasang dan surut lingkungan dengan kadar garam yang tinggi. Oleh karena kemampuan adapatsinya yang unik sehingga mangrove memiliki banyak senyawa bioaktif yang memiliki manfaat secara ekologis, farmakologis, dan toksikologis. (Tong et al., 2006). Di Filipina, hutan mangrove memberikan banyak manfaat bagi kehidupan masyarakat yang hidup di pesisir pantai. Secara tradisional, mangrove digunakan sebagai
Jurnal Ilmu Pertanian Indonesia
Indonesia is a tropical country that has high plant biodiversity. One of the plant groups that ar... more Indonesia is a tropical country that has high plant biodiversity. One of the plant groups that are rich in species are ferns. Ferns still exist until now and estimated at 10.000 species, which 3.000 species are grown out in Indonesia. Watumbolo forest is located in South West Sumba, with the average temperature ranges from 21-34°C, therefore the varians types of plants grow well in this forest, including ferns. The aim of this study was to identify the types of ferns found in the Watumbolo forest. Observations were also made on environmental conditions which included: air temperature, light intensity, soil moisture, and soil pH. This research was conducted using descriptive exploration method. The results obtained 18 species of ferns, namely: Asplenium nidus, Gleichenia linearis, Blechnum orientale, Davallia denticulata, Nephrolepis biserrata, Drynaria quercifolia, Drymoglossum piloselloides, Drynaria parishii, Nephrolepis hirsutula, Angiopteris evecta, Cyclosorus interruptus, Chris...
BIOEDUKASI (Jurnal Pendidikan Biologi)
Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a... more Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a jackfruit crackers and other food product. Jackfruit seed consist of carbohydrate (36,7 g/100 g) and also as a source minerals. The availability of jackfruit seed was found abundance but the use of it not maximize yet. The flour from jackfruit seed can be used an alternative for making food product. The flour extracted from (40,891) jackfruit seed have longer storage time and easy to turn it to other product. The aim of this research was to measure water content, protein content and potassium level from jackfruit flour. The method used during the research was experimental research which is to compare the treatment between boiled and fresh jackfruit seed. The findings showed that water content of boiled jackfruit seed are higher (43,844 unit) than the flour of the fresh jackfruit flour (40,891 unit).The protein content are lowest of flour of boiled jackfruit seed with the value of 7,51 ...
The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natura... more The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natural forests and reforestation sites were compared in three areas from Philippines: Central VisayasBais, Negros Oriental, Alcantara, Cebu and Pangangan Island, Bohol. Three diseases were identified, namely the Brown Leaf Spot disease (BRS), Black Leaf Spot disease (BLS) and White Leaf Spot disease (WLS). BRS was found in Sonneratia alba and Avicennia marina in natural forests and reforestation sites in all areas, while BLS and WLS were found only in Bais, with BLS occurring in Ceriops decandra and Osbornia octodonta in natural forests and in Rhizophora stylosa in reforested sites. WLS occurred only in A. marina in natural forests. Disease prevalence for BRS was significantly higher in reforested areas (F=8.477; p=0.001). 4 fungi genera were consistently found in infected leaves: Aspergillus, Penicillium, Pseudocercosporella and Rhizopus. The occurrence percentage was higher in natural fores...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi, 2021
ABSTRACTPalm trees (Arenga pinnata (wurmb.) Merr.) Are plantation crops that have the potential t... more ABSTRACTPalm trees (Arenga pinnata (wurmb.) Merr.) Are plantation crops that have the potential to be cultivated in the future, because they have high economic value and are prospective for commercial development given the diverse uses. Enau is a plant that was developed from generation to generation on Timor Island. Communities in the village of Nekmese still manage traditional palm trees. Vitamin C or ascorbic acid is one of the vitamins made from hexose derivatives which is water soluble and easily oxidized. The purpose of this study was to determine the vitamin C content of palm meat (A. pinnata). This research was conducted in May 2019 at the TPH Laboratory of the State Polytechnic of Kupang. Determination of vitamin C content in the sample is determined by titration using a solution of iodine. From the results of the study showed that the content of vitamin C in the flesh of the most palm fruit is found at a temperature of 5 ° C and 10 ° C while the content of vitamin C which ...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi, 2021
ABSTRAK Ekosistem lamun merupakan salah satu ekosistem di laut dangkal yang mempuny... more ABSTRAK Ekosistem lamun merupakan salah satu ekosistem di laut dangkal yang mempunyai peranan penting dalam kehidupan biota laut dan merupakan salah satu ekosistem bahari yang paling produktif, sehingga mampu mendukung potensi sumber daya yang tinggi pula. Fungsi ekologis ekosistem lamun adalah sebagai produsen, pendaur unsur hara, penstabil substrat, penangkap sedimen, habitat dan makanan serta tempat berlindung organisme laut lainnya. Tujuan dari penelitian ini adalah, untuk mengidentifikasi pigmen dan mengetahui kandungan pigmen yang terdeteksi pada lamun Enhalus acoroides (Linnaeus f.) di Perairan Pantai Amadoke Desa Akle Kecamatan Semau Selatan Kabupaten Kupang. Metode yang digunakan dalam penelitian ini adalah metode Survei, Kromotografi Lapis Tipis, dan metode Spektrofotometer. Lamun ini mengandung pigmen terlihat pada hasil analisis spektrofotometer pada ekstrak pigmen total lamun dan pemisahan pigmen lewat uji Kromotografi Lapis Tipis (KLT). Dalam proses ekstr...
Acta Aquatica: Aquatic Sciences Journal, 2021
Abstrak Penelitian ini adalah dari melaksanakan Februari hingga April 2017. menyalak dari tujuh ... more Abstrak Penelitian ini adalah dari melaksanakan Februari hingga April 2017. menyalak dari tujuh spesies bakau yaitu ,. Ceriops decandra (Griff.) Ding Hou; Ceriops tagal ( Perr.) CB Rob .; dan Bruguiera cylindrica ; Bruguiera gymnorrhiza (kiri) Lam; Rhizophora stylosa Griff .; Rhizophora mucronata Lam .; dan Avicennia marina (Forsk.) Vierh. Analisis fitokimia dan profil KLT dari ekstrak kasar kulit kayu yang dibandingkan. Analisis kualitatif menunjukkan bahwa Gonggongan Ceriops decandra (Griff.) Ding Hou, Ceriops tagal ( Perr.) CB Rob.,Bruguiera cylindrica, Bru guiera gymnorrhiza ( Kiri ) Lam, Rhizophora stylosa Griff. , Rhizophora mucronata Lam ., Dan Avicennia marina (Forsk.) Vierh. memiliki beberapa metabolit sekunder yang penting, yaitu senyawa Fenol, Tanin, dan Flavonoid. Flavonoid hanya ada di Avicennia marina (Forsk.) Vierh. Profil KLT menunjukkan bahwa dibandingkan DENGAN Nilai R f standar , Terdapat doa Titik hati kromatogram Ekstrak kasar Rhizophora stylosa Griff. sama d...
Society is now vulnerable to various diseases. people need to be educated in the selection of foo... more Society is now vulnerable to various diseases. people need to be educated in the selection of foods that are healthy and good for the body. β-carotene is a healthy food that is good for the body. The study was conducted to determine the content of β-carotene compounds in fruit (Arenga pinata). Samples were obtained from Baumata village. The study used Thin Layer Chromatography (TLC) and Spectrophotometry methods. The research data were analyzed according to linear regression equations and relevant reference studies. The results of the study using the TLC method showed that the Rf value for palm fruit was 0.943 and the Rf value for the standard β-carotene was 0.544. Spectrophotometric test showed the content of β-carotene compounds from palm fruit extract at a wavelength of 470 nm had a concentration of 26.18 ppm with absorbance of 0.055. As for the standard β-carotene concentration of 35.83 ppm with an absorbance value of 0.080. Based on the results of the study it can be concluded ...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi, 2021
ABSTRACTMangrove plant is one of the coastal ecosystems that dominates the coastal area and has t... more ABSTRACTMangrove plant is one of the coastal ecosystems that dominates the coastal area and has the potential to be developed in the prevention of various diseases both infections in aquaculture and as a natural treatment medium for humans, because the secondary metabolite compound contained can be efficacious as an anti-bacterial. The purpose of this study was to determine the effect of A. marina mangrove bark extract against the growth of E. coli and the optimum concentration of A. marina mangrove bark extract in inhibiting the growth of E. coli bacteria. The method used in this study is a laboratory experimental method with 4 treatments namely a concentration of 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL and 1 control without administration of extract with 3 replications. Observation result of inhibition zone diameters were statistically analyzed using a one-way analysis was performed using the SPSS program version 16.0. the results showed that the rough extract of A. marina mangrove...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi
ABSTRACTPumpkin (Cucurbita moschata (Duch.) Poir ) is one of the vegetables that is rich in β-car... more ABSTRACTPumpkin (Cucurbita moschata (Duch.) Poir ) is one of the vegetables that is rich in β-carotene as a precursor of vitamin A and has a fairly high carbohydrate content. Pumpkin processing can be done to produce various kinds of products, one of which is cake. Cake is a baked dough and liquid dough made from flour, sugar, salt, ingredients, shortening, milk, eggs, and aroma enhancer. This study aims to determine the levels of β-carotene contained in pumpkin cake and to determine the acceptability of β-carotene in pumpkin cake. This research is an experimental research. The experimental design used was Completely Randomized Design (CRD) with 1 control 4 treatments and each treatment was repeated 3 times. The research phase consisted of making flour and making cake. Data collection methods include organoleptic tests and β-carotene tests. Data were analyzed using analysis of variance / ANOVA to determine differences in quality, and if there is an influence then proceed with the Tu...
Jurnal Pembelajaran dan Biologi Nukleus (JPBN), Nov 29, 2023
Marine biota has many benefits for human life. Sponges are a species of marine biota that can be ... more Marine biota has many benefits for human life. Sponges are a species of marine biota that can be used as a producer of antimicrobial compounds. The bacteria found in sponges have an important role in the continuity of life in the sea. The symbiotic lifestyle that occurs in bacteria and sponges has the opportunity to form substitutions for the content of secondary metabolites and enzymes, especially extracellular enzymes (amylase, protease, cellulose and lipase). This study aims to determine how to isolate sponge symbiotic bacteria and identify spongy symbiotic bacteria. The method used is purposive to take sponges. Characterization of bacteria was carried out based on morphology and gram staining. Enzymatic bacterial selection was carried out by testing the activity of the amylase enzyme (soluble starch), lipase enzyme (Teen 80), protease enzyme (skim milk) and cellulose enzyme (carboxyl methyl cellulose). Data analysis was performed in a qualitative descriptive manner by measuring the clear zone in the extracellular enzyme test results. Meanwhile, other research parameters measured in this study were temperature, salinity and pH. The results showed that out of 47 isolated bacteria and 33 of them had extracellular enzymes with 10 bacteria had amylase enzymes, 27 bacteria had lipase enzymes, 2 bacteria had protease enzymes and 1 isolate had cellulose enzymes
Jurnal Penelitian Kehutanan Wallacea, Dec 16, 2023
The Taplel waterfall area which is in the Sisimeni Sanam Education and Training Forest area is on... more The Taplel waterfall area which is in the Sisimeni Sanam Education and Training Forest area is one of the conservation areas in East Nusa Tenggara which has quite high biodiversity, including plants that produce natural colors. However, information on the species diversity of dye plants in the Taplel waterfall area is still lacking. The objective of this study was to uncover the diversity of natural dye plants in the Taplel waterfall area, Sisimeni Sanam Training Forest, Takari District, Kupang Regency. Data collection was carried out by setting sample plots measuring 2 x 2 meters (seedlings), 5 x 5 meters (saplings), 10 x 10 meters (poles), and 20 x 20 meters (trees). Analysis of the importance of species using the Importance Value Index, and species diversity using the Species Diversity Index (Shannon-Wiener Index). Species of natural dye plants found in the
Jambura Edu Biosfer Journal, May 22, 2020
Today's society is vulnerable to various diseases. Everyone needs to be given understanding in ch... more Today's society is vulnerable to various diseases. Everyone needs to be given understanding in choosing healthy foods for the body. β-carotene is one of the good compounds contained in vegetables and fruits. This research was conducted to determine the content of β-carotene compounds in fruit (Arenga pinata). Samples were obtained from Baumata village. This research uses thin layer chromatography (TLC) and spectrophotometry methods. The research data were analyzed based on linear regression equations and relevant reference studies. The results of the study using the TLC method showed that the Rf value for palm fruit was 0.943. Spectrophotometric examination showed the content of β-carotene compounds from palm fruit extract at a wavelength of 470 nm had a concentration of 26.18 ppm. Based on the results of these studies it can be concluded that the palm fruit contains β-carotene. Thus palm fruit can be used as one of the choices of healthy food for the community and also as a choice of sources of vitamin A.
Indigenous Biologi, Aug 18, 2021
Palm oil (Arenga pinnata Merr.) is a plantation plant that has the potential to be cultivated in ... more Palm oil (Arenga pinnata Merr.) is a plantation plant that has the potential to be cultivated in the future, because it has high economic value and is prospective for commercial cultivation considering its various uses. Enau is a plant that has been developed from generation to generation on the island of Timor. The community in Nekmese Village manages enau still in the traditional way. Vitamin C or ascorbic acid is one of the vitamins made from hexose derivatives that are soluble in water and easily oxidized. The purpose of this study was to determine the content of vitamin C in the flesh of palm fruit. This research was carried out in May 2019 at the Kupang State Agricultural Polytechnic TPH Laboratory. Determination of the vitamin C content in the sample was determined by titration using an iodine solution. The content of vitamin C in palm fruit flesh based on the temperature treatment of 5°C 10°C and 15°C showed that storage treatment at 5°C had the highest vitamin C content with an average of 4.14 mg followed by storage at 10°C with an average of 3.33 mg and storage at a temperature of 15°C with an average vitamin C content of 3.02 mg. This study also showed that the longer the palm fruit is stored, the lower the vitamin C content in the palm fruit.
Aacl Bioflux, 2019
The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natura... more The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natural forests and reforestation sites were compared in three areas from Philippines: Central Visayas-Bais, Negros Oriental, Alcantara, Cebu and Pangangan Island, Bohol. Three diseases were identified, namely the Brown Leaf Spot disease (BRS), Black Leaf Spot disease (BLS) and White Leaf Spot disease (WLS). BRS was found in Sonneratia alba and Avicennia marina in natural forests and reforestation sites in all areas, while BLS and WLS were found only in Bais, with BLS occurring in Ceriops decandra and Osbornia octodonta in natural forests and in Rhizophora stylosa in reforested sites. WLS occurred only in A. marina in natural forests. Disease prevalence for BRS was significantly higher in reforested areas (F=8.477; p=0.001). 4 fungi genera were consistently found in infected leaves: Aspergillus, Penicillium, Pseudocercosporella and Rhizopus. The occurrence percentage was higher in natural forests than in reforestation sites for all fungi.
Indigenous Biologi, Mar 2, 2021
Tumbuhan mangrove merupakan salah satu ekosistem pantai yang mendominasi wilayah pantai suatu dae... more Tumbuhan mangrove merupakan salah satu ekosistem pantai yang mendominasi wilayah pantai suatu daerah dan berpotensi untuk dikembangkan dalam penanggulangan berbagai penyakit baik infeksi pada ikan budidaya maupun sebagai medi pengobatan alami bagi manusia, karena senyawa metaboit sekunder yang terkandung didalamnya mampu berkhasiat sebagai antibakteri. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak kasar kulit batang mangrove A. marina terhadap pertumbuhan bakteri E. coli dan konsentrasi optimum dari estrak kasar kulit batang mangrove A. marina dalam menghambat pertumbuhan bakteri E. coli. Metode yang digunakan dalam penelitian ini metode eksperimen laboratorium dengan 4 perlakuan yaitu konsentrasi 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL dan 1 kontrol tanpa pemberian ekstrak dengan 3 kali ulangan. Hasil pengamatan diameter zona hambat dianalisis secara statistik menggunakan analisis variansi (ANOVA) satu jalur dengan signifikansi 5%. Analisis statistik dilakukan menggunakan program SPSS versi 16.0. Hasil penelitian menunjukkan bahwa ekstrak kasar kulit batang mangrove A. marina berpengaruh terhadap pertumbuhan bakteri E. coli. Hal ini dapat dilihat dar konsentrasi yang digunakan mulai dari 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL memiliki rata-rata zona hambat yang berkisar antara 26 mm, 27.33 mm, 27.66 mm dan 28.66 mm. Dengan demikian semakin tinggi konsentrasi ekstrak yang digunakan, maka semakin besar daya hambat yang terbentuk.
Bioedukasi : Jurnal Pendidikan Biologi FKIP UM Metro, May 31, 2022
Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a... more Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a jackfruit crackers and other food product. Jackfruit seed consist of carbohydrate (36,7 g/100 g) and also as a source minerals. The availability of jackfruit seed was found abundance but the use of it not maximize yet. The flour from jackfruit seed can be used an alternative for making food product. The flour extracted from (40,891) jackfruit seed have longer storage time and easy to turn it to other product.
Indigenous Biologi, Aug 18, 2021
Labu kuning (Cucurbita moschata (Duch.) Poir) merupakan salah satu sayuran yang kaya akan βkarote... more Labu kuning (Cucurbita moschata (Duch.) Poir) merupakan salah satu sayuran yang kaya akan βkaroten sebagai prekursor vitamin A dan memiliki kandungan karbohidrat yang cukup tinggi. Pengolahan labu kuning dapat dilakukan untuk menghasilkan berbagai macam produk salah satunya cake. Cake merupakan adonan panggang dan adonan liquid yang terbuat dari tepung terigu, gula, garam, bahan pengembang, shortening, susu, telur, dan penambah aroma. Penelitian ini bertujuan untuk mengetahui kadar β-karoten yang terdapat pada cake labu kuning dan untuk mengetahui daya terima β-karoten pada cake labu kuning. Penelitian ini merupakan penelitian eksperimen. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 1 kontrol 4 perlakuan dan masing-masing perlakuan diulang sebanyak 3 kali, selain β-Karoten yang diuji 2 kali. Tahap penelitian terdiri dari pembuatan tepung dan pembuatan cake. Metode pengumpulan data meliputi uji organoleptik dan uji β-karoten. Data dianalisis menggunakan analisa varian/ANOVA untuk mengetahui perbedaan kualitas, dan apabila terdapat pengaruh maka dilanjutkan dengan uji Tukey. Hasil penelitian uji organoleptik menunjukan bahwa pada parameter warna tidak memberikan pengaruh yang nyata, sehingga tidak dilanjutkan dengan uji Tukey, dari segi aroma yaitu A1 merupakan perlakuan terbaik dengan nilai 3,56, dari segi tekstur perlakuan terbaik adalah A1 dengan nilai 4,04 dan dari segi rasa perlakuan terbaik adalah A1 dengan nilai 3,80. Untuk uji β-karoten, perlakuan cake labu kuning dengan kadar β-karoten tertinggi terdapat pada A4 dengan rata-rata 0,262 dan kadar terendah terdapat pada A0 dengan rata-rata 0,018. Berdasarkan data hasil penelitian, tepung labu kuning layak dijadikan salah satu sumber tepung alternatif karena cake yang dihasilkan memenuhi standar mutu SNI baik dari segi warna, rasa, aroma, tekstur maupun kadar β-karoten.
Acta Aquatica, Mar 25, 2021
Mangrove didefinisikan sebagai pohon dan semak yang memiliki kemanpuan untuk tumbuh di pesisir pa... more Mangrove didefinisikan sebagai pohon dan semak yang memiliki kemanpuan untuk tumbuh di pesisir pantai. Mangrove tumbuh di lingkungan dengan kondisi lingkungan ekstrim seperti daerah yang berlumpur mendapat hempasan ombak pasang dan surut lingkungan dengan kadar garam yang tinggi. Oleh karena kemampuan adapatsinya yang unik sehingga mangrove memiliki banyak senyawa bioaktif yang memiliki manfaat secara ekologis, farmakologis, dan toksikologis. (Tong et al., 2006). Di Filipina, hutan mangrove memberikan banyak manfaat bagi kehidupan masyarakat yang hidup di pesisir pantai. Secara tradisional, mangrove digunakan sebagai
Jurnal Ilmu Pertanian Indonesia
Indonesia is a tropical country that has high plant biodiversity. One of the plant groups that ar... more Indonesia is a tropical country that has high plant biodiversity. One of the plant groups that are rich in species are ferns. Ferns still exist until now and estimated at 10.000 species, which 3.000 species are grown out in Indonesia. Watumbolo forest is located in South West Sumba, with the average temperature ranges from 21-34°C, therefore the varians types of plants grow well in this forest, including ferns. The aim of this study was to identify the types of ferns found in the Watumbolo forest. Observations were also made on environmental conditions which included: air temperature, light intensity, soil moisture, and soil pH. This research was conducted using descriptive exploration method. The results obtained 18 species of ferns, namely: Asplenium nidus, Gleichenia linearis, Blechnum orientale, Davallia denticulata, Nephrolepis biserrata, Drynaria quercifolia, Drymoglossum piloselloides, Drynaria parishii, Nephrolepis hirsutula, Angiopteris evecta, Cyclosorus interruptus, Chris...
BIOEDUKASI (Jurnal Pendidikan Biologi)
Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a... more Jackfruit seed is usually thrown away after being consumed as fresh fruit or being processed as a jackfruit crackers and other food product. Jackfruit seed consist of carbohydrate (36,7 g/100 g) and also as a source minerals. The availability of jackfruit seed was found abundance but the use of it not maximize yet. The flour from jackfruit seed can be used an alternative for making food product. The flour extracted from (40,891) jackfruit seed have longer storage time and easy to turn it to other product. The aim of this research was to measure water content, protein content and potassium level from jackfruit flour. The method used during the research was experimental research which is to compare the treatment between boiled and fresh jackfruit seed. The findings showed that water content of boiled jackfruit seed are higher (43,844 unit) than the flour of the fresh jackfruit flour (40,891 unit).The protein content are lowest of flour of boiled jackfruit seed with the value of 7,51 ...
The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natura... more The prevalence of fungal infections and the occurrence of fungi in mangrove leaves from in natural forests and reforestation sites were compared in three areas from Philippines: Central VisayasBais, Negros Oriental, Alcantara, Cebu and Pangangan Island, Bohol. Three diseases were identified, namely the Brown Leaf Spot disease (BRS), Black Leaf Spot disease (BLS) and White Leaf Spot disease (WLS). BRS was found in Sonneratia alba and Avicennia marina in natural forests and reforestation sites in all areas, while BLS and WLS were found only in Bais, with BLS occurring in Ceriops decandra and Osbornia octodonta in natural forests and in Rhizophora stylosa in reforested sites. WLS occurred only in A. marina in natural forests. Disease prevalence for BRS was significantly higher in reforested areas (F=8.477; p=0.001). 4 fungi genera were consistently found in infected leaves: Aspergillus, Penicillium, Pseudocercosporella and Rhizopus. The occurrence percentage was higher in natural fores...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi, 2021
ABSTRACTPalm trees (Arenga pinnata (wurmb.) Merr.) Are plantation crops that have the potential t... more ABSTRACTPalm trees (Arenga pinnata (wurmb.) Merr.) Are plantation crops that have the potential to be cultivated in the future, because they have high economic value and are prospective for commercial development given the diverse uses. Enau is a plant that was developed from generation to generation on Timor Island. Communities in the village of Nekmese still manage traditional palm trees. Vitamin C or ascorbic acid is one of the vitamins made from hexose derivatives which is water soluble and easily oxidized. The purpose of this study was to determine the vitamin C content of palm meat (A. pinnata). This research was conducted in May 2019 at the TPH Laboratory of the State Polytechnic of Kupang. Determination of vitamin C content in the sample is determined by titration using a solution of iodine. From the results of the study showed that the content of vitamin C in the flesh of the most palm fruit is found at a temperature of 5 ° C and 10 ° C while the content of vitamin C which ...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi, 2021
ABSTRAK Ekosistem lamun merupakan salah satu ekosistem di laut dangkal yang mempuny... more ABSTRAK Ekosistem lamun merupakan salah satu ekosistem di laut dangkal yang mempunyai peranan penting dalam kehidupan biota laut dan merupakan salah satu ekosistem bahari yang paling produktif, sehingga mampu mendukung potensi sumber daya yang tinggi pula. Fungsi ekologis ekosistem lamun adalah sebagai produsen, pendaur unsur hara, penstabil substrat, penangkap sedimen, habitat dan makanan serta tempat berlindung organisme laut lainnya. Tujuan dari penelitian ini adalah, untuk mengidentifikasi pigmen dan mengetahui kandungan pigmen yang terdeteksi pada lamun Enhalus acoroides (Linnaeus f.) di Perairan Pantai Amadoke Desa Akle Kecamatan Semau Selatan Kabupaten Kupang. Metode yang digunakan dalam penelitian ini adalah metode Survei, Kromotografi Lapis Tipis, dan metode Spektrofotometer. Lamun ini mengandung pigmen terlihat pada hasil analisis spektrofotometer pada ekstrak pigmen total lamun dan pemisahan pigmen lewat uji Kromotografi Lapis Tipis (KLT). Dalam proses ekstr...
Acta Aquatica: Aquatic Sciences Journal, 2021
Abstrak Penelitian ini adalah dari melaksanakan Februari hingga April 2017. menyalak dari tujuh ... more Abstrak Penelitian ini adalah dari melaksanakan Februari hingga April 2017. menyalak dari tujuh spesies bakau yaitu ,. Ceriops decandra (Griff.) Ding Hou; Ceriops tagal ( Perr.) CB Rob .; dan Bruguiera cylindrica ; Bruguiera gymnorrhiza (kiri) Lam; Rhizophora stylosa Griff .; Rhizophora mucronata Lam .; dan Avicennia marina (Forsk.) Vierh. Analisis fitokimia dan profil KLT dari ekstrak kasar kulit kayu yang dibandingkan. Analisis kualitatif menunjukkan bahwa Gonggongan Ceriops decandra (Griff.) Ding Hou, Ceriops tagal ( Perr.) CB Rob.,Bruguiera cylindrica, Bru guiera gymnorrhiza ( Kiri ) Lam, Rhizophora stylosa Griff. , Rhizophora mucronata Lam ., Dan Avicennia marina (Forsk.) Vierh. memiliki beberapa metabolit sekunder yang penting, yaitu senyawa Fenol, Tanin, dan Flavonoid. Flavonoid hanya ada di Avicennia marina (Forsk.) Vierh. Profil KLT menunjukkan bahwa dibandingkan DENGAN Nilai R f standar , Terdapat doa Titik hati kromatogram Ekstrak kasar Rhizophora stylosa Griff. sama d...
Society is now vulnerable to various diseases. people need to be educated in the selection of foo... more Society is now vulnerable to various diseases. people need to be educated in the selection of foods that are healthy and good for the body. β-carotene is a healthy food that is good for the body. The study was conducted to determine the content of β-carotene compounds in fruit (Arenga pinata). Samples were obtained from Baumata village. The study used Thin Layer Chromatography (TLC) and Spectrophotometry methods. The research data were analyzed according to linear regression equations and relevant reference studies. The results of the study using the TLC method showed that the Rf value for palm fruit was 0.943 and the Rf value for the standard β-carotene was 0.544. Spectrophotometric test showed the content of β-carotene compounds from palm fruit extract at a wavelength of 470 nm had a concentration of 26.18 ppm with absorbance of 0.055. As for the standard β-carotene concentration of 35.83 ppm with an absorbance value of 0.080. Based on the results of the study it can be concluded ...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi, 2021
ABSTRACTMangrove plant is one of the coastal ecosystems that dominates the coastal area and has t... more ABSTRACTMangrove plant is one of the coastal ecosystems that dominates the coastal area and has the potential to be developed in the prevention of various diseases both infections in aquaculture and as a natural treatment medium for humans, because the secondary metabolite compound contained can be efficacious as an anti-bacterial. The purpose of this study was to determine the effect of A. marina mangrove bark extract against the growth of E. coli and the optimum concentration of A. marina mangrove bark extract in inhibiting the growth of E. coli bacteria. The method used in this study is a laboratory experimental method with 4 treatments namely a concentration of 55 mg/mL, 60 mg/mL, 65 mg/mL, 70 mg/mL and 1 control without administration of extract with 3 replications. Observation result of inhibition zone diameters were statistically analyzed using a one-way analysis was performed using the SPSS program version 16.0. the results showed that the rough extract of A. marina mangrove...
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi
ABSTRACTPumpkin (Cucurbita moschata (Duch.) Poir ) is one of the vegetables that is rich in β-car... more ABSTRACTPumpkin (Cucurbita moschata (Duch.) Poir ) is one of the vegetables that is rich in β-carotene as a precursor of vitamin A and has a fairly high carbohydrate content. Pumpkin processing can be done to produce various kinds of products, one of which is cake. Cake is a baked dough and liquid dough made from flour, sugar, salt, ingredients, shortening, milk, eggs, and aroma enhancer. This study aims to determine the levels of β-carotene contained in pumpkin cake and to determine the acceptability of β-carotene in pumpkin cake. This research is an experimental research. The experimental design used was Completely Randomized Design (CRD) with 1 control 4 treatments and each treatment was repeated 3 times. The research phase consisted of making flour and making cake. Data collection methods include organoleptic tests and β-carotene tests. Data were analyzed using analysis of variance / ANOVA to determine differences in quality, and if there is an influence then proceed with the Tu...