Angelo Biânchi - Academia.edu (original) (raw)

Papers by Angelo Biânchi

Research paper thumbnail of BACK MATTER

Maize Genetics and Breeding in the 20th Century, 1999

Research paper thumbnail of FRONT MATTER

Maize Genetics and Breeding in the 20th Century, 1999

Research paper thumbnail of Maize Genetics and Breeding in the 20th Century

This book provides the biographies, and a related summary, of geneticists and breeders of maize w... more This book provides the biographies, and a related summary, of geneticists and breeders of maize who have contributed to the major discoveries in the 20th century. Their relationships to one another, as well as the general developments in maize genetics and breeding growth, are ...

Research paper thumbnail of Maize Genetics and Breeding in the 20th Century

This book provides the biographies, and a related summary, of geneticists and breeders of maize w... more This book provides the biographies, and a related summary, of geneticists and breeders of maize who have contributed to the major discoveries in the 20th century. Their relationships to one another, as well as the general developments in maize genetics and breeding growth, are ...

Research paper thumbnail of Screening for hemostasis during hemorrhagic emergencies

International Journal of Clinical & Laboratory Research, 1989

We propose a laboratory screening scheme for the hemostatic system to be adopted during hemorrhag... more We propose a laboratory screening scheme for the hemostatic system to be adopted during hemorrhagic emergencies in hospital patients bleeding excessively to the extent of requiring massive blood transfusions. The aim of the screening scheme is to establish whether excessive bleeding is due to alterations of the hemostatic system or to other causes. Seven tests were chosen on the basis of their simplicity, rapidity and comprehensiveness in the evaluation of the hemostatic system: the platelet count, the prothrombin and activated partial thromboplastin times, the thrombin and reptilase times and the assays of plasma fibrinogen and fibrin(ogen) degradation products. We then attempted to validate the choice of these tests with 172 emergency cases due to excessive bleeding which led to massive blood replacement. The high frequency of abnormalities of one or more tests found in this series (93%) indicates the excellent diagnostic sensitivity of the screening scheme in detecting hemostatic abnormalities. The screening scheme was also useful in the diagnostic work-up of the bleeding disorders most frequently encountered during hemorrhagic emergencies (disseminated intravascular coagulation, liver disease, unsuspected heparinization and the hemostatic defect associated with massive blood transfusion).

Research paper thumbnail of The genetic base of congenital thrombotic thrombocytopenic purpura (TTP)

Research paper thumbnail of Correcting the Genetically Determined Sterility of * Male Mice

Research paper thumbnail of The first deletion mutation in the TSP1-6 repeat domain of ADAMTS13 in a family with inherited thrombotic thrombocytopenic purpura

haematologica | 2008; 94(2) | 289 | © F e r r a t a S t o r t i F o u n d a t i o n R. Palla et a... more haematologica | 2008; 94(2) | 289 | © F e r r a t a S t o r t i F o u n d a t i o n R. Palla et al. | 290 | haematologica | 2009; 94(2)

Research paper thumbnail of Missense or splicing mutation? The case of a fibrinogen B-chain mutation causing severe hypofibrinogenemia

Research paper thumbnail of The thrombospondin-1 N700S polymorphism is associated with early myocardial infarction without altering von Willebrand Factor multimer size

Research paper thumbnail of Thrombotic thrombocytopenic purpura and the hemolytic uremic syndrome: much progress and many remaining issues

Haematologica-the Hematology Journal, 2007

Research paper thumbnail of Unusual forms of venous thrombosis and thrombophilia

Pathophysiology of Haemostasis and Thrombosis, 2002

Venous thromboembolism (VTE) results from multiple interactions between inherited and environment... more Venous thromboembolism (VTE) results from multiple interactions between inherited and environmental risk factors. The lower limbs are the most common site of VTE, but more rarely other venous sites can be involved. The role of risk factors for VTE can be differ- ent in the various thrombotic manifestations, and there are specific risk factors for specific sites. Coagulation abnormalities causing

Research paper thumbnail of Epitope mapping of ADAMTS13 autoantibodies in acquired thrombotic thrombocytopenic purpura

Severe deficiency of the von Willebrand factor (VWF)-cleaving protease ADAMTS13 can lead to throm... more Severe deficiency of the von Willebrand factor (VWF)-cleaving protease ADAMTS13 can lead to thrombotic thrombocytopenic purpura (TTP), a disease associated with the widespread formation of platelet-rich thrombi in many organs. Autoantibodies that inactivate ADAMTS13 are the most frequent cause of acquired TTP. Little is known about epitope specificity and reactivity of anti-ADAMTS13 antibodies. In this study, a series of ADAMTS13 domains were expressed in Escherichia coli, and the reactivity of purified recombinant fragments with anti-ADAMTS13 auto-antibodies from 25 patients with severe ADAMTS13 deficiency was evaluated in vitro. All TTP plasmas contained antibodies directed against the cysteine-rich spacer (cys-rich/spacer) domain of ADAMTS13. In the plasma of 3 patients, antibodies were detected that reacted exclusively with the cys-rich/spacer domain, underscoring the importance of this region for functional activity of ADAMTS13. In 64% of the plasmas, antibodies reacted with the 2 CUB domains, and in 56% they reacted with the isolated first thrombospondin type 1 (TSP-1) repeat and with the compound fragment consisting of the catalytic, the disintegrin-like, and the TSP1-1 domain. Less frequently, in 28% of the plasmas, antibodies reacted with the TSP1 repeats 2 to 8. Unexpectedly, antibodies reacted with the propeptide region in 20% of the plasmas. In conclusion, this study shows that even though anti-ADAMTS13 autoantibodies react with multiple domains of the protease, the cys-rich/spacer domain is consistently involved in antibody reactivity.

Research paper thumbnail of Running head: MARTINELLI et al. Hyperhomocysteinemia in cerebral vein thrombosis

Research paper thumbnail of Letter Regarding Article by Martinelli et al, "Risk Factors and Recurrence Rate of Primary Deep Vein Thrombosis of the Upper Extremities" * Response

Research paper thumbnail of ADAMTS13 and anti-ADAMTS13 antibodies as markers for recurrence of acquired thrombotic thrombocytopenic purpura during remission

From 20 to 50% of patients who survive an acute episode of the acquired form of thrombotic thromb... more From 20 to 50% of patients who survive an acute episode of the acquired form of thrombotic thrombocytopenic purpura relapse but clinical and laboratory markers of recurrence are not well established.

Research paper thumbnail of Nonsense-mediated mRNA decay in the ADAMTS13 gene caused b y a 29-nucleotide deletion

In mammalian cells a regulatory mechanism, known as nonsense-mediated mRNA decay, degrades mRNA h... more In mammalian cells a regulatory mechanism, known as nonsense-mediated mRNA decay, degrades mRNA harboring premature termination codons. This mechanism is intron-dependent and functions as a quality control mechanism to eliminate abnormal transcripts and modulates the levels of a variety of naturally occurring transcripts.

Research paper thumbnail of An excellent material for cytogenetic studies from maize-teosinte derivatives

Genetica, 1959

Although numerous and sometime superb cytogenetical studies have been performed with maize, as fa... more Although numerous and sometime superb cytogenetical studies have been performed with maize, as far as I know not very many strains have been demonstratively indicated as imparting excellent spreading qualities to pachitene smears from pollen mother cells, which are the ...

Research paper thumbnail of Guía de estilo

Research paper thumbnail of BACK MATTER

Maize Genetics and Breeding in the 20th Century, 1999

Research paper thumbnail of FRONT MATTER

Maize Genetics and Breeding in the 20th Century, 1999

Research paper thumbnail of Maize Genetics and Breeding in the 20th Century

This book provides the biographies, and a related summary, of geneticists and breeders of maize w... more This book provides the biographies, and a related summary, of geneticists and breeders of maize who have contributed to the major discoveries in the 20th century. Their relationships to one another, as well as the general developments in maize genetics and breeding growth, are ...

Research paper thumbnail of Maize Genetics and Breeding in the 20th Century

This book provides the biographies, and a related summary, of geneticists and breeders of maize w... more This book provides the biographies, and a related summary, of geneticists and breeders of maize who have contributed to the major discoveries in the 20th century. Their relationships to one another, as well as the general developments in maize genetics and breeding growth, are ...

Research paper thumbnail of Screening for hemostasis during hemorrhagic emergencies

International Journal of Clinical & Laboratory Research, 1989

We propose a laboratory screening scheme for the hemostatic system to be adopted during hemorrhag... more We propose a laboratory screening scheme for the hemostatic system to be adopted during hemorrhagic emergencies in hospital patients bleeding excessively to the extent of requiring massive blood transfusions. The aim of the screening scheme is to establish whether excessive bleeding is due to alterations of the hemostatic system or to other causes. Seven tests were chosen on the basis of their simplicity, rapidity and comprehensiveness in the evaluation of the hemostatic system: the platelet count, the prothrombin and activated partial thromboplastin times, the thrombin and reptilase times and the assays of plasma fibrinogen and fibrin(ogen) degradation products. We then attempted to validate the choice of these tests with 172 emergency cases due to excessive bleeding which led to massive blood replacement. The high frequency of abnormalities of one or more tests found in this series (93%) indicates the excellent diagnostic sensitivity of the screening scheme in detecting hemostatic abnormalities. The screening scheme was also useful in the diagnostic work-up of the bleeding disorders most frequently encountered during hemorrhagic emergencies (disseminated intravascular coagulation, liver disease, unsuspected heparinization and the hemostatic defect associated with massive blood transfusion).

Research paper thumbnail of The genetic base of congenital thrombotic thrombocytopenic purpura (TTP)

Research paper thumbnail of Correcting the Genetically Determined Sterility of * Male Mice

Research paper thumbnail of The first deletion mutation in the TSP1-6 repeat domain of ADAMTS13 in a family with inherited thrombotic thrombocytopenic purpura

haematologica | 2008; 94(2) | 289 | © F e r r a t a S t o r t i F o u n d a t i o n R. Palla et a... more haematologica | 2008; 94(2) | 289 | © F e r r a t a S t o r t i F o u n d a t i o n R. Palla et al. | 290 | haematologica | 2009; 94(2)

Research paper thumbnail of Missense or splicing mutation? The case of a fibrinogen B-chain mutation causing severe hypofibrinogenemia

Research paper thumbnail of The thrombospondin-1 N700S polymorphism is associated with early myocardial infarction without altering von Willebrand Factor multimer size

Research paper thumbnail of Thrombotic thrombocytopenic purpura and the hemolytic uremic syndrome: much progress and many remaining issues

Haematologica-the Hematology Journal, 2007

Research paper thumbnail of Unusual forms of venous thrombosis and thrombophilia

Pathophysiology of Haemostasis and Thrombosis, 2002

Venous thromboembolism (VTE) results from multiple interactions between inherited and environment... more Venous thromboembolism (VTE) results from multiple interactions between inherited and environmental risk factors. The lower limbs are the most common site of VTE, but more rarely other venous sites can be involved. The role of risk factors for VTE can be differ- ent in the various thrombotic manifestations, and there are specific risk factors for specific sites. Coagulation abnormalities causing

Research paper thumbnail of Epitope mapping of ADAMTS13 autoantibodies in acquired thrombotic thrombocytopenic purpura

Severe deficiency of the von Willebrand factor (VWF)-cleaving protease ADAMTS13 can lead to throm... more Severe deficiency of the von Willebrand factor (VWF)-cleaving protease ADAMTS13 can lead to thrombotic thrombocytopenic purpura (TTP), a disease associated with the widespread formation of platelet-rich thrombi in many organs. Autoantibodies that inactivate ADAMTS13 are the most frequent cause of acquired TTP. Little is known about epitope specificity and reactivity of anti-ADAMTS13 antibodies. In this study, a series of ADAMTS13 domains were expressed in Escherichia coli, and the reactivity of purified recombinant fragments with anti-ADAMTS13 auto-antibodies from 25 patients with severe ADAMTS13 deficiency was evaluated in vitro. All TTP plasmas contained antibodies directed against the cysteine-rich spacer (cys-rich/spacer) domain of ADAMTS13. In the plasma of 3 patients, antibodies were detected that reacted exclusively with the cys-rich/spacer domain, underscoring the importance of this region for functional activity of ADAMTS13. In 64% of the plasmas, antibodies reacted with the 2 CUB domains, and in 56% they reacted with the isolated first thrombospondin type 1 (TSP-1) repeat and with the compound fragment consisting of the catalytic, the disintegrin-like, and the TSP1-1 domain. Less frequently, in 28% of the plasmas, antibodies reacted with the TSP1 repeats 2 to 8. Unexpectedly, antibodies reacted with the propeptide region in 20% of the plasmas. In conclusion, this study shows that even though anti-ADAMTS13 autoantibodies react with multiple domains of the protease, the cys-rich/spacer domain is consistently involved in antibody reactivity.

Research paper thumbnail of Running head: MARTINELLI et al. Hyperhomocysteinemia in cerebral vein thrombosis

Research paper thumbnail of Letter Regarding Article by Martinelli et al, "Risk Factors and Recurrence Rate of Primary Deep Vein Thrombosis of the Upper Extremities" * Response

Research paper thumbnail of ADAMTS13 and anti-ADAMTS13 antibodies as markers for recurrence of acquired thrombotic thrombocytopenic purpura during remission

From 20 to 50% of patients who survive an acute episode of the acquired form of thrombotic thromb... more From 20 to 50% of patients who survive an acute episode of the acquired form of thrombotic thrombocytopenic purpura relapse but clinical and laboratory markers of recurrence are not well established.

Research paper thumbnail of Nonsense-mediated mRNA decay in the ADAMTS13 gene caused b y a 29-nucleotide deletion

In mammalian cells a regulatory mechanism, known as nonsense-mediated mRNA decay, degrades mRNA h... more In mammalian cells a regulatory mechanism, known as nonsense-mediated mRNA decay, degrades mRNA harboring premature termination codons. This mechanism is intron-dependent and functions as a quality control mechanism to eliminate abnormal transcripts and modulates the levels of a variety of naturally occurring transcripts.

Research paper thumbnail of An excellent material for cytogenetic studies from maize-teosinte derivatives

Genetica, 1959

Although numerous and sometime superb cytogenetical studies have been performed with maize, as fa... more Although numerous and sometime superb cytogenetical studies have been performed with maize, as far as I know not very many strains have been demonstratively indicated as imparting excellent spreading qualities to pachitene smears from pollen mother cells, which are the ...

Research paper thumbnail of Guía de estilo