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Papers by Anindita Chakraborty

Journal of Toxicology, 2018

In recent years, natural bioactive components draw a major attention for their potent anticarcino... more In recent years, natural bioactive components draw a major attention for their potent anticarcinogenic activity. Cinnamon, one of the traditional spices, most frequently used in almost every household of tropical countries has got enormous efficacy to combat cancer. Cinnamon as a whole and/or its active components exhibited significant antineoplastic activity in different types of cancer. This review has been carried out to elicit the molecular mechanisms of action of cinnamon and its components on oncogenic regulators and related pathways. Web sites of Google Scholar, Medline, and PubMed were searched for articles written in English and published in peer-reviewed journals from 2003 to 2017. The anticarcinogenic potential of cinnamon varies with the type of cancer and also depends on the administered active compound individually or in combination with some chemicals or even extract of cinnamon as a whole. Some of its active components exert chemosensitization of well-known anticance...

Management of Hazardous Wastes, 2016

A packed bed bioreactor efficiently treated low-level radioactive waste for years with a retentio... more A packed bed bioreactor efficiently treated low-level radioactive waste for years with a retention time of 24 h using acetate as the sole carbon source. However, there was generation of dead biomass. This bioreactor biomass was used to develop a bacterial consortium, which could perform the function within 4 h while simultaneously accumulating nitrate and phosphate. The dead mass was negligible. Serial dilution technique was used to isolate the world's first pure culture of a nitrate accumulating strain from this consortium. This isolate could simultaneously accumulate nitrate and phosphate from solution. Its ability to form biofilm helped develop a packed bed bioreactor system for waste water treatment, which could optimally remove 94.46% nitrate within 11 h in batch mode while 8 h in continuous mode from waste water starting from 275 ppm of nitrate. The conventional approach revealed the strain to be a member of genus Bacillus but showed distinct differences with the type strains. Further insilico analysis of the draft genome and the putative protein sequences using the bioinformatics tools revealed the strain to be a novel variant of genus Bacillus. The sequestered nitrate and phosphate within the cell were visualized through electron microscopy and explained the reason behind the ability of the isolate to accumulate 1.12

Ecotoxicology, 2014

In the present study the response of antioxidant enzyme activities and the level of expression of... more In the present study the response of antioxidant enzyme activities and the level of expression of their corresponding genes on bioaccumulation of iron (Fe) were investigated. In vitro germinated Withania somnifera L. were grown in Murashige and Skoog's liquid medium with increasing concentrations (0, 25, 50, 100 and 200 lM) of FeSO 4 for 7 and 14 days. Root and leaf tissues analyzed for catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1) and guaiacol peroxidase (GPX, EC 1.11.1.7), have shown an increase in content with respect to exposure time. Isoforms of CAT, SOD and GPX were separated using non-denaturing polyacrylamide gel electrophoresis and observed that the isoenzymes were greatly affected by higher concentrations of Fe. Reverse transcriptase polymerase chain reaction analysis performed by taking three pairs of genes of CAT (RsCat, Catalase1, Cat1) and SOD (SodCp, TaSOD1.2, MnSOD) to find out the differential expression of antioxidant genes under Fe excess. RsCat from CAT and MnSOD from SOD have exhibited high levels of gene expression under Fe stress, which was consistent with the changes of the activity assayed in solution after 7 days of treatment. Analysis by proton induced X-ray emission exhibited an increasing uptake of Fe in plants by suppressing and expressing of other nutrient elements. The results of the present study suggest that higher concentration of Fe causes disturbance in nutrient balance and induces oxidative stress in plant. Keywords Antioxidant enzymes Á Bioaccumulation Á Gene expression Á Iron stress Á Proton induced X-ray emission (PIXE) Á Withania somnifera L.

International Journal of Radiation Biology, 2013

To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and ... more To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in Plantago ovata Forsk (P. ovata). Chlorophyll content was estimated to study the stress response in P. ovata seedlings following exposure to gamma irradiation and CdCl2. Lipid peroxidation and proline content, two oxidative stress markers, were also studied. The level of metallothionein gene and protein expression was further investigated using polymerase chain reaction (PCR), reverse transcription-PCR (RT-PCR), immunocytochemistry and flow cytometry. Three MT genes of P. ovata namely PoMT 1, PoMT 2 and PoMT 3 were isolated, sequenced and characterized and their expressions were found to be altered in the case of both oxidative stresses in a dose-dependent and tissue-specific manner. The results were in agreement with the observations from immunocytochemistry and FACS analysis. The results suggest that both gamma irradiation and CdCl2 alter redox balance in P. ovata. The metallothionein gene may play an important role in metal tolerance and stress balance. It is conjectured that the stress-mediated imbalance is maintained by altered MT gene and protein expression.

Ecological Indicators, 2014

Levels of air pollution can be assessed through passive and active monitoring. However, due to se... more Levels of air pollution can be assessed through passive and active monitoring. However, due to several drawbacks of active monitoring recently much emphasis is being given to the passive monitoring of air-pollution. Street dust and the dusts deposited over the leaves of roadside trees can be considered a valuable indicator of environmental pollution. Present study was undertaken to analyze the size distribution and investigate the elemental and mineralogical components of the street dusts and the dusts deposited on the leaves of roadside trees. Samples were collected from three representative areas of metro-city Kolkata, India, considering the urban characteristics and traffic profile. X-ray powder diffraction (XRD) was selected to detect major mineral components. The size distribution of dust particles was observed under scanning electron microscope (SEM) and the elemental distribution was analyzed using SEM attached to energy dispersive X ray spectrometer (SEM-EDX). X-ray diffraction analysis has revealed the presence of mineral components like quartz and various clay minerals such as calcite, gypsum, albite, microcline, chlorite, muscovite and oxides of iron. Elemental analysis has shown the presence of elements like Na, Mg, Al, Si, Cl, K, Ca, Fe, and Zn. Variations in size, mineral and elemental composition were observed. From the study it is evident that the information on mineralogical and elemental composition of street dusts and dusts deposited on the leave surfaces of roadside trees can act as indicator of air-pollution.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2008

Proton induced X-ray emission was used to study the trace elemental profiles of embryogenic and n... more Proton induced X-ray emission was used to study the trace elemental profiles of embryogenic and non-embryogenic callus of an important cash crop of India-Plantago ovata. Somatic embryogenesis, a well-known process for plant regeneration and crop improvement is modulated by various factors such as ionizing radiation and micro nutrients in the growth media. The present work reports the trace element variation in normal and irradiated callus tissue of P. ovata. Embryogenic and non-embryogenic callus tissues were exposed to gamma rays from a 60 Co gamma source. The absorbed dose ranged from 10 to 100 Gy. Subsequent experiments showed significant dose dependent alterations in K, Ca, Mn, Fe, Ni, Cu, Zn, Br, Sr in both the embryogenic and non-embryogenic callus. The precise involvement of these elements has been discussed in light of somatic embryogenesis of the selected medicinal plant.

Zinc causes toxicity to the plants in an excess concentration and it is manifested by chlorosis, ... more Zinc causes toxicity to the plants in an excess concentration and it is manifested by chlorosis, rolling of leaf margins, and disruption of membrane integrity. The heavy metal stress also triggers the stimulation of enzymatic and non-enzymatic antioxidant systems. Polyamines are naturally occurring, secondary metabolites, protecting plants from heavy metal-induced stress. Plants also up-regulate the mRNA expression of Metallothionein in response to heavy metal-induced oxidative stress. The alteration in Metallothionein type 2 (PoMT2) expression of a medicinally important herb Plantago ovata in presence of polyamines like Putrescine, Spermidine, and Spermine in addition to ZnSO4.H2O by the semi-quantitative and the quantitative methods have been demonstrated in the present study. We have observed reductions in the expression of the Metallothionein type 2 gene in the presence of the aforementioned polyamines which implies their protective and antioxidant properties to fight against th...

Critical Reviews in Environmental Science and Technology, 2015

Title-A review on air pollution monitoring and management using plants with special reference to ... more Title-A review on air pollution monitoring and management using plants with special reference to foliar dust adsorption and physiological stress responses

African Journal of Biological Sciences

Momordica charantia is a medicinal plant comprising of antidiabetic properties. Along with this, ... more Momordica charantia is a medicinal plant comprising of antidiabetic properties. Along with this, it has been recognized as a good source of trace elements which are beneficial for health. So it is important to find the actual trace element content in different M. charantia fruits cultivated in different areas. We have found 12 elements varying within the fruit parts where the fruit pulp was found to be key reservoir of calcium, manganese, potassium and sulfur. Charantin, being one of the important antidiabetic compounds found in M. charantia, were estimated using HPLC and found to be accumulated at maximum in the Tamluk region. Squalene synthase is the rate limiting enzyme in phytosterol biosynthesis and therefore, we have characterized the M. charantia Squalene Synthase (McSQS) gene, went through an in silico study and tried to find the expression correlation with charantin content in M. charantia fruits, where we found that McSQS expression is highest in Tamluk region.

Bioremediation Journal, 2022

Plant Cell, Tissue and Organ Culture (PCTOC), 2022

Modulatory role of quercetin against gamma radiation mediated biochemical and morphological alter... more Modulatory role of quercetin against gamma radiation mediated biochemical and morphological alterations of red blood cells

Chemosphere, 2021

Hexavalent chromium is a highly toxic element generated due to indiscriminate chromite mining in ... more Hexavalent chromium is a highly toxic element generated due to indiscriminate chromite mining in Sukinda, Odisha. In the present research investigation a relatively higher Cr(VI) resistant (900 mg L-1) bacterium CWB-54 was isolated from the chromite mine water. Based on the biochemical and molecular analysis the strain (CWB-54) was identified as Exiguobacterium mexicanum. When this bacterium was grown at 35 °C, 100 rpm, pH~8.0, and fructose as an electron donor, it could reduce the total hexavalent chromium (100 mg L-1) supplemented in the medium within 33 h of incubation period. Though experiment was carried out to study the effect of Mn, Ni, Cd, Hg and Zn on Cr(VI) reduction by the strain E. mexicanum it has been observed that in the presence of Cd and Hg, Cr(VI) reduction drastically decreased. Characterization of Cr(VI) reduced product by SEM-EDX and TEM analysis revealed intracellular and extracellular Cr(III) deposition in the bacterium, which is assumed to be Cr(OH)3 precipitate in nanometric size. But the extracellular chromate reductase enzyme production is found to be negligible as compared to the intracellular enzyme production. The increased concentration of Cr(VI) above (1000 mg L-1) also showed the genotoxic effect on the DNA. Several reports have been published on Exiguobacterium sp. on different scientific aspect but the current report on the reduction of toxic Cr(VI) by a new species E. mexicanum is a novel one which established the potentiality of this microorganism for a broad area of application.

International Journal of Radiation Biology, 2019

She has a Bachelors and Masters degree in Zoology. Her research interests are molecular biology, ... more She has a Bachelors and Masters degree in Zoology. Her research interests are molecular biology, stress biology, radiation induced bystander effects, radiation and cancer. Anindita Chakraborty Dr. Anindita Chakraborty, Scientist G is the Head of Stress Biology Lab at UGC-DAE Consortium for Scientific Research, Kolkata Centre. Her research program focuses on assessment of the stress-effects in cellular processes and functional manifestation of stress exposed life forms in terms of altered metabolism. She has a Bachelor and Masters Degree in Zoology, M. Phil in Environmental Science and PhD in Biochemistry with more than 25 years of research experience devoted to areas of cancer biochemistry, stress biology and molecular dynamics.

Water Environment Research, 2013

This study describes the potential use of steel-making slag as an arsenic-removal medium. Systema... more This study describes the potential use of steel-making slag as an arsenic-removal medium. Systematic analysis of slag material revealed a composition of oxides of calcium, iron, silicon, and phosphorous. Under the experimental conditions of this study, the equilibrium time was shown to be 2 hours, and the removal capacity to be 99%, with an adsorbent loading capacity of 1.25g/l. The adsorption kinetics were shown to follow a pseudo-second-order rate equation, and the adsorption isotherm closely followed both the Langmuir and Freundlich isothermic models. Variations in solution pH levels demonstrated that with a decrease in the initial solution pH, the adsorption capacity decreases. This is attributed to the leaching of silica and phosphate from the slag to the solution, which imparted a competing effect for adsorption sites. However, with an alkaline pH, such leaching was reduced, and due to formation of calcium carbonate from the leached calcium from the slag material, the arsenic removal efficiency increased as it was co-precipitated with calcium carbonate.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2000

Particle-induced X-ray emission (PIXE) analysis was employed in the present study to investigate ... more Particle-induced X-ray emission (PIXE) analysis was employed in the present study to investigate the chemopreventive potential of vanadium in influencing trace elemental profile and antioxidant status in chemical carcinogenesis. The elements with Z=15–40 were studied. Data reveal remarkable alterations in elemental composition in the hepatic tissue of diethylnitrosamine (DENA)-induced Sprague–Dawley male rats (intraperitoneal (ip) dose: 200 mg/kg body weight) after

Food Chemistry, 2009

This work describes radiation-induced effects on edible seed protein profiles, carbohydrates, ami... more This work describes radiation-induced effects on edible seed protein profiles, carbohydrates, amino acids and genomic DNA during gamma sterilization. The total protein and carbohydrate was decreased with increasing dose compared to control samples. Oryza sativa L. Cv-2233 exhibited a minimum effect in terms of its loss in total soluble protein content, compared to other seeds at 6 kGy, and the soluble protein fraction, containing 14-16 kDa albumins and 22 kDa globulin, was unchanged up to 6 kGy. In Cicer arietinum, the effect of gamma rays was more pronounced on albumin and prolamin with respect to glutelin and globulin. The easy-to-digest and difficult-to-digest proteins were not significantly affected up to 4 kGy. However, the soluble free amino acids of all the seeds increased with increasing dose. The total DNA content and band intensities both decreased with increasing absorbed dose; however, the band positions were unchanged for all seed types.

Chemosphere, 2014

h i g h l i g h t s Identification of B. amyloliquefaciens by 16S rRNA gene sequencing from chrom... more h i g h l i g h t s Identification of B. amyloliquefaciens by 16S rRNA gene sequencing from chromite soil. Cr(VI) reduction potential of B. amyloliquefaciens is a first report. Bacterium reduces 100 mg L À1 of Cr(VI) in a faster rate of 45 h under optimized set of condition. Reduced product was characterized with SEM-EDX, Raman spectroscopy, TEM-EDX, SAED, XRD, FT-IR, AAS analyses. Nanometric sized amorphous Cr(III) hydroxide have industrial applications.

RSC Advances, 2016

Naringin ameliorates the toxic effects of radiation in murine liver and decreases the oxidative s... more Naringin ameliorates the toxic effects of radiation in murine liver and decreases the oxidative stress through the modulation of redox-regulated cellular signaling system.

Carbohydrate polymers, Jan 15, 2018

Computational analyses followed by traditional wet-bench experiments have become a method of choi... more Computational analyses followed by traditional wet-bench experiments have become a method of choice due to successful results. To enhance the solubility and bioavailability of curcumin within chitosan nanoparticle, we have exploited computational methodologies i.e. docking, BBD-RSM and MD simulation for the polymer selection, NPs' formulation, optimization and their stability confirmation in an aqueous medium, respectively. Formulated CSCur NPs were assessed for in-vitro release, which exhibited a sustained release pattern and four-fold higher cytotoxic activity in a nanoparticulated system. Enhanced uptake, apoptotic effect of CSCur NPs were established by morphological changes in cells as observed by fluorescence microscopy and FE-SEM. DNA damage, cell-cycle blockage and elevated ROS levels further confirm the anticancer activity of the CSCur NPs following apoptotic pathways. In-vivo study on Danio rerio, for uptake and toxicity reveal the particle's biocompatibility and n...

Journal of Toxicology, 2018

In recent years, natural bioactive components draw a major attention for their potent anticarcino... more In recent years, natural bioactive components draw a major attention for their potent anticarcinogenic activity. Cinnamon, one of the traditional spices, most frequently used in almost every household of tropical countries has got enormous efficacy to combat cancer. Cinnamon as a whole and/or its active components exhibited significant antineoplastic activity in different types of cancer. This review has been carried out to elicit the molecular mechanisms of action of cinnamon and its components on oncogenic regulators and related pathways. Web sites of Google Scholar, Medline, and PubMed were searched for articles written in English and published in peer-reviewed journals from 2003 to 2017. The anticarcinogenic potential of cinnamon varies with the type of cancer and also depends on the administered active compound individually or in combination with some chemicals or even extract of cinnamon as a whole. Some of its active components exert chemosensitization of well-known anticance...

Management of Hazardous Wastes, 2016

A packed bed bioreactor efficiently treated low-level radioactive waste for years with a retentio... more A packed bed bioreactor efficiently treated low-level radioactive waste for years with a retention time of 24 h using acetate as the sole carbon source. However, there was generation of dead biomass. This bioreactor biomass was used to develop a bacterial consortium, which could perform the function within 4 h while simultaneously accumulating nitrate and phosphate. The dead mass was negligible. Serial dilution technique was used to isolate the world's first pure culture of a nitrate accumulating strain from this consortium. This isolate could simultaneously accumulate nitrate and phosphate from solution. Its ability to form biofilm helped develop a packed bed bioreactor system for waste water treatment, which could optimally remove 94.46% nitrate within 11 h in batch mode while 8 h in continuous mode from waste water starting from 275 ppm of nitrate. The conventional approach revealed the strain to be a member of genus Bacillus but showed distinct differences with the type strains. Further insilico analysis of the draft genome and the putative protein sequences using the bioinformatics tools revealed the strain to be a novel variant of genus Bacillus. The sequestered nitrate and phosphate within the cell were visualized through electron microscopy and explained the reason behind the ability of the isolate to accumulate 1.12

Ecotoxicology, 2014

In the present study the response of antioxidant enzyme activities and the level of expression of... more In the present study the response of antioxidant enzyme activities and the level of expression of their corresponding genes on bioaccumulation of iron (Fe) were investigated. In vitro germinated Withania somnifera L. were grown in Murashige and Skoog's liquid medium with increasing concentrations (0, 25, 50, 100 and 200 lM) of FeSO 4 for 7 and 14 days. Root and leaf tissues analyzed for catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1) and guaiacol peroxidase (GPX, EC 1.11.1.7), have shown an increase in content with respect to exposure time. Isoforms of CAT, SOD and GPX were separated using non-denaturing polyacrylamide gel electrophoresis and observed that the isoenzymes were greatly affected by higher concentrations of Fe. Reverse transcriptase polymerase chain reaction analysis performed by taking three pairs of genes of CAT (RsCat, Catalase1, Cat1) and SOD (SodCp, TaSOD1.2, MnSOD) to find out the differential expression of antioxidant genes under Fe excess. RsCat from CAT and MnSOD from SOD have exhibited high levels of gene expression under Fe stress, which was consistent with the changes of the activity assayed in solution after 7 days of treatment. Analysis by proton induced X-ray emission exhibited an increasing uptake of Fe in plants by suppressing and expressing of other nutrient elements. The results of the present study suggest that higher concentration of Fe causes disturbance in nutrient balance and induces oxidative stress in plant. Keywords Antioxidant enzymes Á Bioaccumulation Á Gene expression Á Iron stress Á Proton induced X-ray emission (PIXE) Á Withania somnifera L.

International Journal of Radiation Biology, 2013

To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and ... more To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in Plantago ovata Forsk (P. ovata). Chlorophyll content was estimated to study the stress response in P. ovata seedlings following exposure to gamma irradiation and CdCl2. Lipid peroxidation and proline content, two oxidative stress markers, were also studied. The level of metallothionein gene and protein expression was further investigated using polymerase chain reaction (PCR), reverse transcription-PCR (RT-PCR), immunocytochemistry and flow cytometry. Three MT genes of P. ovata namely PoMT 1, PoMT 2 and PoMT 3 were isolated, sequenced and characterized and their expressions were found to be altered in the case of both oxidative stresses in a dose-dependent and tissue-specific manner. The results were in agreement with the observations from immunocytochemistry and FACS analysis. The results suggest that both gamma irradiation and CdCl2 alter redox balance in P. ovata. The metallothionein gene may play an important role in metal tolerance and stress balance. It is conjectured that the stress-mediated imbalance is maintained by altered MT gene and protein expression.

Ecological Indicators, 2014

Levels of air pollution can be assessed through passive and active monitoring. However, due to se... more Levels of air pollution can be assessed through passive and active monitoring. However, due to several drawbacks of active monitoring recently much emphasis is being given to the passive monitoring of air-pollution. Street dust and the dusts deposited over the leaves of roadside trees can be considered a valuable indicator of environmental pollution. Present study was undertaken to analyze the size distribution and investigate the elemental and mineralogical components of the street dusts and the dusts deposited on the leaves of roadside trees. Samples were collected from three representative areas of metro-city Kolkata, India, considering the urban characteristics and traffic profile. X-ray powder diffraction (XRD) was selected to detect major mineral components. The size distribution of dust particles was observed under scanning electron microscope (SEM) and the elemental distribution was analyzed using SEM attached to energy dispersive X ray spectrometer (SEM-EDX). X-ray diffraction analysis has revealed the presence of mineral components like quartz and various clay minerals such as calcite, gypsum, albite, microcline, chlorite, muscovite and oxides of iron. Elemental analysis has shown the presence of elements like Na, Mg, Al, Si, Cl, K, Ca, Fe, and Zn. Variations in size, mineral and elemental composition were observed. From the study it is evident that the information on mineralogical and elemental composition of street dusts and dusts deposited on the leave surfaces of roadside trees can act as indicator of air-pollution.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2008

Proton induced X-ray emission was used to study the trace elemental profiles of embryogenic and n... more Proton induced X-ray emission was used to study the trace elemental profiles of embryogenic and non-embryogenic callus of an important cash crop of India-Plantago ovata. Somatic embryogenesis, a well-known process for plant regeneration and crop improvement is modulated by various factors such as ionizing radiation and micro nutrients in the growth media. The present work reports the trace element variation in normal and irradiated callus tissue of P. ovata. Embryogenic and non-embryogenic callus tissues were exposed to gamma rays from a 60 Co gamma source. The absorbed dose ranged from 10 to 100 Gy. Subsequent experiments showed significant dose dependent alterations in K, Ca, Mn, Fe, Ni, Cu, Zn, Br, Sr in both the embryogenic and non-embryogenic callus. The precise involvement of these elements has been discussed in light of somatic embryogenesis of the selected medicinal plant.

Zinc causes toxicity to the plants in an excess concentration and it is manifested by chlorosis, ... more Zinc causes toxicity to the plants in an excess concentration and it is manifested by chlorosis, rolling of leaf margins, and disruption of membrane integrity. The heavy metal stress also triggers the stimulation of enzymatic and non-enzymatic antioxidant systems. Polyamines are naturally occurring, secondary metabolites, protecting plants from heavy metal-induced stress. Plants also up-regulate the mRNA expression of Metallothionein in response to heavy metal-induced oxidative stress. The alteration in Metallothionein type 2 (PoMT2) expression of a medicinally important herb Plantago ovata in presence of polyamines like Putrescine, Spermidine, and Spermine in addition to ZnSO4.H2O by the semi-quantitative and the quantitative methods have been demonstrated in the present study. We have observed reductions in the expression of the Metallothionein type 2 gene in the presence of the aforementioned polyamines which implies their protective and antioxidant properties to fight against th...

Critical Reviews in Environmental Science and Technology, 2015

Title-A review on air pollution monitoring and management using plants with special reference to ... more Title-A review on air pollution monitoring and management using plants with special reference to foliar dust adsorption and physiological stress responses

African Journal of Biological Sciences

Momordica charantia is a medicinal plant comprising of antidiabetic properties. Along with this, ... more Momordica charantia is a medicinal plant comprising of antidiabetic properties. Along with this, it has been recognized as a good source of trace elements which are beneficial for health. So it is important to find the actual trace element content in different M. charantia fruits cultivated in different areas. We have found 12 elements varying within the fruit parts where the fruit pulp was found to be key reservoir of calcium, manganese, potassium and sulfur. Charantin, being one of the important antidiabetic compounds found in M. charantia, were estimated using HPLC and found to be accumulated at maximum in the Tamluk region. Squalene synthase is the rate limiting enzyme in phytosterol biosynthesis and therefore, we have characterized the M. charantia Squalene Synthase (McSQS) gene, went through an in silico study and tried to find the expression correlation with charantin content in M. charantia fruits, where we found that McSQS expression is highest in Tamluk region.

Bioremediation Journal, 2022

Plant Cell, Tissue and Organ Culture (PCTOC), 2022

Modulatory role of quercetin against gamma radiation mediated biochemical and morphological alter... more Modulatory role of quercetin against gamma radiation mediated biochemical and morphological alterations of red blood cells

Chemosphere, 2021

Hexavalent chromium is a highly toxic element generated due to indiscriminate chromite mining in ... more Hexavalent chromium is a highly toxic element generated due to indiscriminate chromite mining in Sukinda, Odisha. In the present research investigation a relatively higher Cr(VI) resistant (900 mg L-1) bacterium CWB-54 was isolated from the chromite mine water. Based on the biochemical and molecular analysis the strain (CWB-54) was identified as Exiguobacterium mexicanum. When this bacterium was grown at 35 °C, 100 rpm, pH~8.0, and fructose as an electron donor, it could reduce the total hexavalent chromium (100 mg L-1) supplemented in the medium within 33 h of incubation period. Though experiment was carried out to study the effect of Mn, Ni, Cd, Hg and Zn on Cr(VI) reduction by the strain E. mexicanum it has been observed that in the presence of Cd and Hg, Cr(VI) reduction drastically decreased. Characterization of Cr(VI) reduced product by SEM-EDX and TEM analysis revealed intracellular and extracellular Cr(III) deposition in the bacterium, which is assumed to be Cr(OH)3 precipitate in nanometric size. But the extracellular chromate reductase enzyme production is found to be negligible as compared to the intracellular enzyme production. The increased concentration of Cr(VI) above (1000 mg L-1) also showed the genotoxic effect on the DNA. Several reports have been published on Exiguobacterium sp. on different scientific aspect but the current report on the reduction of toxic Cr(VI) by a new species E. mexicanum is a novel one which established the potentiality of this microorganism for a broad area of application.

International Journal of Radiation Biology, 2019

She has a Bachelors and Masters degree in Zoology. Her research interests are molecular biology, ... more She has a Bachelors and Masters degree in Zoology. Her research interests are molecular biology, stress biology, radiation induced bystander effects, radiation and cancer. Anindita Chakraborty Dr. Anindita Chakraborty, Scientist G is the Head of Stress Biology Lab at UGC-DAE Consortium for Scientific Research, Kolkata Centre. Her research program focuses on assessment of the stress-effects in cellular processes and functional manifestation of stress exposed life forms in terms of altered metabolism. She has a Bachelor and Masters Degree in Zoology, M. Phil in Environmental Science and PhD in Biochemistry with more than 25 years of research experience devoted to areas of cancer biochemistry, stress biology and molecular dynamics.

Water Environment Research, 2013

This study describes the potential use of steel-making slag as an arsenic-removal medium. Systema... more This study describes the potential use of steel-making slag as an arsenic-removal medium. Systematic analysis of slag material revealed a composition of oxides of calcium, iron, silicon, and phosphorous. Under the experimental conditions of this study, the equilibrium time was shown to be 2 hours, and the removal capacity to be 99%, with an adsorbent loading capacity of 1.25g/l. The adsorption kinetics were shown to follow a pseudo-second-order rate equation, and the adsorption isotherm closely followed both the Langmuir and Freundlich isothermic models. Variations in solution pH levels demonstrated that with a decrease in the initial solution pH, the adsorption capacity decreases. This is attributed to the leaching of silica and phosphate from the slag to the solution, which imparted a competing effect for adsorption sites. However, with an alkaline pH, such leaching was reduced, and due to formation of calcium carbonate from the leached calcium from the slag material, the arsenic removal efficiency increased as it was co-precipitated with calcium carbonate.

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2000

Particle-induced X-ray emission (PIXE) analysis was employed in the present study to investigate ... more Particle-induced X-ray emission (PIXE) analysis was employed in the present study to investigate the chemopreventive potential of vanadium in influencing trace elemental profile and antioxidant status in chemical carcinogenesis. The elements with Z=15–40 were studied. Data reveal remarkable alterations in elemental composition in the hepatic tissue of diethylnitrosamine (DENA)-induced Sprague–Dawley male rats (intraperitoneal (ip) dose: 200 mg/kg body weight) after

Food Chemistry, 2009

This work describes radiation-induced effects on edible seed protein profiles, carbohydrates, ami... more This work describes radiation-induced effects on edible seed protein profiles, carbohydrates, amino acids and genomic DNA during gamma sterilization. The total protein and carbohydrate was decreased with increasing dose compared to control samples. Oryza sativa L. Cv-2233 exhibited a minimum effect in terms of its loss in total soluble protein content, compared to other seeds at 6 kGy, and the soluble protein fraction, containing 14-16 kDa albumins and 22 kDa globulin, was unchanged up to 6 kGy. In Cicer arietinum, the effect of gamma rays was more pronounced on albumin and prolamin with respect to glutelin and globulin. The easy-to-digest and difficult-to-digest proteins were not significantly affected up to 4 kGy. However, the soluble free amino acids of all the seeds increased with increasing dose. The total DNA content and band intensities both decreased with increasing absorbed dose; however, the band positions were unchanged for all seed types.

Chemosphere, 2014

h i g h l i g h t s Identification of B. amyloliquefaciens by 16S rRNA gene sequencing from chrom... more h i g h l i g h t s Identification of B. amyloliquefaciens by 16S rRNA gene sequencing from chromite soil. Cr(VI) reduction potential of B. amyloliquefaciens is a first report. Bacterium reduces 100 mg L À1 of Cr(VI) in a faster rate of 45 h under optimized set of condition. Reduced product was characterized with SEM-EDX, Raman spectroscopy, TEM-EDX, SAED, XRD, FT-IR, AAS analyses. Nanometric sized amorphous Cr(III) hydroxide have industrial applications.

RSC Advances, 2016

Naringin ameliorates the toxic effects of radiation in murine liver and decreases the oxidative s... more Naringin ameliorates the toxic effects of radiation in murine liver and decreases the oxidative stress through the modulation of redox-regulated cellular signaling system.

Carbohydrate polymers, Jan 15, 2018

Computational analyses followed by traditional wet-bench experiments have become a method of choi... more Computational analyses followed by traditional wet-bench experiments have become a method of choice due to successful results. To enhance the solubility and bioavailability of curcumin within chitosan nanoparticle, we have exploited computational methodologies i.e. docking, BBD-RSM and MD simulation for the polymer selection, NPs' formulation, optimization and their stability confirmation in an aqueous medium, respectively. Formulated CSCur NPs were assessed for in-vitro release, which exhibited a sustained release pattern and four-fold higher cytotoxic activity in a nanoparticulated system. Enhanced uptake, apoptotic effect of CSCur NPs were established by morphological changes in cells as observed by fluorescence microscopy and FE-SEM. DNA damage, cell-cycle blockage and elevated ROS levels further confirm the anticancer activity of the CSCur NPs following apoptotic pathways. In-vivo study on Danio rerio, for uptake and toxicity reveal the particle's biocompatibility and n...