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Papers by Anita Kulloli

Research paper thumbnail of Comparative Evaluation of Efficacy of Antibiotics incorporated Platelet Rich Fibrin Versus Platelet Rich Fibrin alone in the Treatment of Intrabony Defects

Journal of Population Therapeutics and Clinical Pharmacology

Research paper thumbnail of Effect of non-surgical periodontal therapy on superoxide dismutase levels in gingival tissues of chronic periodontitis patients: a clinical and spectophotometric analysis

DOAJ (DOAJ: Directory of Open Access Journals), 2013

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical,... more BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O .− 2), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O .− 2 radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients. METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24-55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gingival index, bleeding index, plaque index. SOD activities were assessed spectrophotometrically at baseline and 2 months post NSPT, results were analysed statistically. RESULTS: At baseline, patients with chronic periodontitis had higher mean SOD activity (2.73 ± 1.36) than the control subjects (1.12 ± 1.13) with p = 0.00003 (p < 0.05). At 2 months post NSPT median SOD level (1.00) had come close to median SOD value of control group (0.85); p = 0.99 (p > 0.05). The resolution of inflammation with successful NSPT resulted in decreased SOD levels as in control group. Clinical parameters in patients with chronic periodontitis showed a significant improvement 2 months post NSPT (p < 0.05). CONCLUSION: Non-surgical periodontal therapy significantly improves the clinical parameters and restores previously increased SOD levels to normal in chronic periodontitis patients.

Research paper thumbnail of Comparative evaluation of clinical efficacy of systemically administered commercially available antioxidant formulations as an adjunct to scaling and root planing in the treatment of chronic periodontitis

Asian Journal of Pharmaceutical Research and Health Care, 2023

Research paper thumbnail of Comparative Evaluation of Coronally Advanced Flap with and without Bioactive Glass Putty in the Management of Gingival Recession Defects: A Randomized Controlled Clinical Trial

PubMed, Jan 14, 2016

Background: The need-of-the-hour is a material that can support coronally advanced flap (CAF) pro... more Background: The need-of-the-hour is a material that can support coronally advanced flap (CAF) procedures in treatment of gingival recession. Recent literature shows that various bone substitutes are being used for this procedure. This study clinically evaluates the outcomes of CAF with and without bioactive glass putty (NovaBone®) in terms of root coverage, gains in keratinized tissue height, and root coverage esthetic score in multiple gingival recession defects. Methods: Ten healthy patients (age range 18-45 years) with multiple bilateral (n = 40 test 20; control 20) and comparable Miller's Class I or Class II gingival recession defects were selected. The defects were randomly assigned by a computer-generated list to either test (CAF + bioactive glass putty) or control (CAF alone) groups. Clinical parameters included gingival recession (GR), probing pocket depth (PPD), clinical attachment level (CAL), keratinized tissue height (KTH), root coverage esthetic score (RES) evaluated at baseline and at 6 months post-surgery CAF with or without bioactive glass putty. Results: Six months post-surgery all clinical parameters showed significant reductions. Gingival recession showed significant reduction both in test and control groups (2.0 ± 0.47 mm and 2.3 ± 0.48 mm, respectively; p < 0.05) with no intergroup difference. The exposed root was covered by 72% (test) and 79% (control). CAL gain was also significant in both groups (test: 2.7 ± 0.67 mm; control: 2.8 ± 0.78 mm; p < 0.05) with no intergroup difference. Keratinized tissue height gain was significant in both the groups (test group: 1.2 ± 0.42 mm; control group: 0.9 ± 0.57 mm) with no intergroup difference. Also, the RES was significant for both the test and control groups (7.2 ± 2.78 and 7.7 ± 1.41 respectively) with no intergroup differences. Conclusions: In isolated Class I/II GR defects, CAF associated with bioactive glass putty provided no significant difference in root coverage, CAL, KTH or RES compared to CAF alone. However, statistically significant gains were seen in all the parameters in both groups as compared to baseline. We refute the claims of the recent studies using a bone substitute for root coverage. Further long-term clinical trials are warranted to substantiate our results.

Research paper thumbnail of Effect of Non-Surgical Periodontal Therapy on Superoxide Dismutase Levels in Gingival Tissues of Chronic Periodontitis Patients: A Clinical and Spectophotometric Analysis

Disease Markers, 2013

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical,... more BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O .− 2), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O .− 2 radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients. METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24-55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gingival index, bleeding index, plaque index. SOD activities were assessed spectrophotometrically at baseline and 2 months post NSPT, results were analysed statistically. RESULTS: At baseline, patients with chronic periodontitis had higher mean SOD activity (2.73 ± 1.36) than the control subjects (1.12 ± 1.13) with p = 0.00003 (p < 0.05). At 2 months post NSPT median SOD level (1.00) had come close to median SOD value of control group (0.85); p = 0.99 (p > 0.05). The resolution of inflammation with successful NSPT resulted in decreased SOD levels as in control group. Clinical parameters in patients with chronic periodontitis showed a significant improvement 2 months post NSPT (p < 0.05). CONCLUSION: Non-surgical periodontal therapy significantly improves the clinical parameters and restores previously increased SOD levels to normal in chronic periodontitis patients.

Research paper thumbnail of Comparative evaluation of platelet-rich fibrin biomaterial and open flap debridement in the treatment of two and three wall intrabony defects

PubMed, Apr 1, 2015

Background: Platelet-rich concentrates are the most widely used regenerative biomaterials. Stimul... more Background: Platelet-rich concentrates are the most widely used regenerative biomaterials. Stimulation and acceleration of soft and hard tissue healing are due to local and continuous delivery of growth factors and proteins, mimicking the needs of the physiological wound healing and reparative tissue processes. This article aims to evaluate the clinical efficacy of open flap debridement (OFD) with or without platelet-rich fibrin (PRF) in the treatment of intrabony defects. Materials and methods: Twenty subjects with forty intrabony defects were treated with either autologous PRF with open-flap debridement (test, n = 20) or open-flap debridement alone (control, n = 20). Soft tissue parameters included: Plaque index, sulcus bleeding index, probing depth, relative attachment level and gingival marginal level (GML). The hard tissue parameters included-distances from: Cement enamel junction to the base of the defect (CEJ-BOD): Alveolar crest to the base of the defect (AC-BOD): And CEJ to AC. The parameters were recorded at baseline and at 9 months postoperatively calculated using standardized radiographs by image-analysis software. Results: Statistically significant (0.005*) intragroup improvements were seen with all the hard and soft parameters in both test and control groups, except for GML. Statistically significant improvements were seen with the mean defect fill (CEJ-BOD and AC-BOD) (P = 0.003*) when intergroup comparisons were made. Conclusions: Adjunctive use of PRF with OFD significantly improves defect fill when compared to OFD alone. PRF has consistently been showing regenerative potential; it is simple, easy and inexpensive biomaterial compared with bone grafts.

Research paper thumbnail of Comparative evaluation of clinical efficacy of systemically administered commercially available antioxidant formulations as an adjunct to scaling and root planing in the treatment of chronic periodontitis

Asian Journal of Pharmaceutical Research and Health Care

Research paper thumbnail of Efficacy of One Stage Full Mouth Disinfection on the Oral Health-Related Quality of Life in Patients with Generalised Chronic Periodontitis

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH

Introduction: Intraoral translocation of periodontal pathogens from untreated intraoral niches ca... more Introduction: Intraoral translocation of periodontal pathogens from untreated intraoral niches can lead to recolonisation and reinfection of recently treated periodontal pockets. Poor oral health hampers the patient’s Quality of Life (QoL) as it causes social isolation, inferiority and the development of psychiatric conditions. Full Mouth Disinfection (FMD) performed within 24 hours using chlorhexidine has reported significant improvements in clinical, microbiological and psychological parameters when compared to conventional periodontal treatment. Aim: To investigate the long-term effect of FMD on Oral HealthRelated Quality of Life (OHRQoL) in patients with generalised chronic periodontitis. Materials and Methods: This study was a prospective clinical study conducted in the Department of Periodontology, Dr DY Patil Dental College and Hospital, Pune, Maharashtra, India, from October 2019 to December 2021. Study consisted of 60 participants who were allotted into two groups; 30 perio...

Research paper thumbnail of Evaluation of Efficacy of Locally Delivered 10% Azadirachta indica (Neem) Gel as an Adjunct to Scaling and Root Planing in the Treatment of Chronic Periodontitis Patients with Type II Diabetes Mellitus: Clinico-Microbiological Study

International Journal of Medical Research and Health Sciences, 2021

Research paper thumbnail of Efficacy of natural tooth pontic in periodontally compromised patients: A systematic review

Journal of Dental Research and Review, 2021

Periodontitis is a major concern for the dentist because the destruction of periodontal fibers ca... more Periodontitis is a major concern for the dentist because the destruction of periodontal fibers causes mobility of teeth and eventually leads to tooth loss. Conventional techniques like removable temporary acrylic prosthesis, or resin-bonded bridges hold their own disadvantages and a fixed prosthesis may worsen the existing condition. Natural tooth pontic (NTP) in such cases may be advantageous in terms of esthetics and patient satisfaction along with cost effectiveness. Hence, the aim of this review was to evaluate the efficacy of NTP in periodontally compromised patients. Electronic search on data sources such as PubMed and Google scholar along with hand searching of the articles was done in institutional library. All case reports and case series were included in English language published till October 31, 2019, were included. After applying inclusion and exclusion criteria, ten articles were selected for the present review. The whole process was conducted by following the Preferre...

Research paper thumbnail of Unmasking Oral Malodor : A Review

Oral malodor is one of the major growing concern of patients today as it is fundamental to their ... more Oral malodor is one of the major growing concern of patients today as it is fundamental to their overall personality. Although the majority of malodor is of oral origin, there are multiple other systemic causes that have to be addressed. Correct diagnosis of the cause of the malodor and prompt treatment can render the patient satisfied. There have been newer developments in faster and more efficient detection of the levels of malodor, and an array of treatment options are present in the market today. It is crucial for the dentist to have a sound understanding of this prevalent oral condition and provide effective intervention programs. This article reviews the various causes and the diagnostic modalities which will help us treat this multifaceted condition.

Research paper thumbnail of Estimation of Beta-CTXin gingival crevicular fluid and serum of periodontally healthy individuals and chronic periodontitis patients before and after non-surgical periodontal therapy- An in-vivo study

IP International Journal of Periodontology and Implantology, 2019

Objective and Design: Beta Cross-linked C-terminal telopeptide of type I collagen (fragment β- CT... more Objective and Design: Beta Cross-linked C-terminal telopeptide of type I collagen (fragment β- CTX) is newer addition to the list of bone resorption markers in serum. The purpose of our study was to estimate and compare the levels β-CTX in gingival crevicular fluid (GCF) and serum of periodontally healthy individuals and chronic periodontitis (CP) patients before and after nonsurgical periodontal therapy (NSPT). Materials and Methods: Twenty Five subjects with equal distribution of males and females were divided into two groups i.e Group I: 10 Periodontally healthy individuals, Group II: 15 CP patients aged between 30-50 years. GCF and serum samples were collected at baseline in Group I whereas in Group II GCF and serum samples were collected at baseline and 2 months after NSPT. The levels of GCF and serum β-CTX were quantified using enzyme-linked immunosorbent assay (ELISA). The clinical parameters evaluated were plaque index (PI), gingival index (GI), probing pocket depth (PPD) and clinical attachment level (CAL) and the correlations of β-CTX in GCF and serum with clinical parameters were evaluated. Results: The mean concentration of β-CTX increased from Group I to Group II. The mean concentration of β-CTX in GCF and serum was reduced after 2 months of NSPT. Furthermore, reduction in β-CTX levels was positively correlated to gain in CAL in GCF. Conclusion: Periodontal treatment resulted in the reduction of β-CTX levels suggesting that β- CTX can be considered as a potential marker of bone resorption in CP.

Research paper thumbnail of Management of internal and external resorption with open apex

Journal of Interdisciplinary Dentistry, 2019

Tooth resorption is a phenomenon resulting in the loss of tooth structure. A simultaneous occurre... more Tooth resorption is a phenomenon resulting in the loss of tooth structure. A simultaneous occurrence of internal resorption and communicating external resorption is often challenging for a clinician. To elicit immediate root canal treatment is of utmost importance, followed by the repair of defect to achieve promising results. For successful outcome, thorough investigations and obtaining a three-dimensional view of the extent and location of resorption should be obtained in order to plan the treatment.

Research paper thumbnail of Efficacy of free gingival graft in the treatment of Miller Class I and Class II localized gingival recessions: A systematic review

Journal of Indian Society of Periodontology, 2019

Background: Currently, the leading theme in mucogingival surgery is the correction of gingival re... more Background: Currently, the leading theme in mucogingival surgery is the correction of gingival recession defects. Free gingival graft (FGG) has been successfully in use in this category of reconstructive therapeutic modality. Objectives: The aim of this systematic review was to evaluate the literature with respect to efficacy of FGG in the management of Miller Class I and II localized gingival recessions. Data Sources: Search strategies were performed via electronic database which included Pubmed-Medline, Google scholar and manual search using University library resources. Two reviewers assessed the eligibility of the studies. Study Eligibility Criteria: Controlled clinical trials, randomized clinical trials and longitudinal studies evaluating recession areas treated by FGG with minimum of 6 months follow up were included. In-vitro and animal studies, studies mainly done on Miller Class III and IV gingival recession defect, studies on multiple gingival recessions and case series and case reports were excluded from the search. Results: The electronic and manual search identified a total of 557 articles. A final screen consisted of 39 articles out of which 17 articles were selected for full-text assessment. Finally, 7 articles were selected for detailed evaluation for this systematic review. FGG has shown significant results in all the studies except for one study. Conclusion: FGG produces substantial results, however, highly depends on the case selection and operator's skill and experience. FGG gives an impression of being the best alternative option in zones where gingival recession presents with inadequate width of attached gingiva and depth of vestibular fornix.

Research paper thumbnail of Sub‐epithelial connective tissue graft for the management of Miller's class I and class II isolated gingival recession defect: A systematic review of the factors influencing the outcome

Journal of Investigative and Clinical Dentistry, 2018

The aim of the present systematic review was to analyze the factors that affect the outcome of su... more The aim of the present systematic review was to analyze the factors that affect the outcome of subepithelial connective tissue graft (SCTG) for managing Miller's class I and class II isolated gingival recession defect. The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses) guidelines for systematic reviews were used. Quality assessments of selected articles were performed. Data on root surface condition, recession type defect, flap thickness, different flap designs, different harvesting techniques, presence/absence of the epithelial collar, graft thickness, flap tension, suturing techniques, and smoking‐related outcomes on root coverage were assessed. The SCTG procedure provides the best root coverage outcomes for Miller's class I and class II recession. The critical threshold of flap thickness was found to be 1 mm. Maximum root coverage was achieved by envelope and modified tunnel technique. SCTG with the epithelial collar does not provide additiona...

Research paper thumbnail of Comparative Study of Measurement Variability using An Electronic Probe and A Manual Probe – “An Eye Opener” - An In Vitro Study

Journal of the Indian Dental Association, Feb 28, 2012

A periodontal probe is considered as an eye below the gingival sulcus. It is widely used to measu... more A periodontal probe is considered as an eye below the gingival sulcus. It is widely used to measure both the probing depth and the attachment loss in order to assess periodontal destruction. However, the use of the periodontal probe particularly in estimating probing depths is subject to large errors. The dimension of the probe tip, the force applied, angle of insertion and root contour all influence the depth recorded. Probing is highly subjected to inter and intra examiner variability and variations in probing force is evident between different examiners. The aim of this study was to compare the relative inter and intra examiner reproducibility of two different periodontal probes – Electronic (florida probe) and the Manual (UNC – 15 probe). Duplicate pocket depth recordings were obtained by three examiners of a single incisor, premolar and molar for each subject at 6 sites. Teeth were equally distributed between the arches and incorporated both shallow (getter than 5mm) and deeper sites (lesser than 5mm) as measured with manual probe. Measurements were repeated 1 week later to avoid bias due to examiner memory.

Research paper thumbnail of Oral telangiectatic granuloma with an intrabony defect

Journal of Indian Society of Periodontology, 2015

Oral telangiectatic granuloma is a benign hyperplastic lesion occurring in response to trauma or ... more Oral telangiectatic granuloma is a benign hyperplastic lesion occurring in response to trauma or chronic irritation in the oral cavity. The characteristic histological appearance comprises of typical granulation tissue with a proliferation of small thin-walled blood vessels in the loose connective tissue. We describe a case of a 36-year-old female who had a swelling in the left maxillary region which was associated with the intrabony defect. An internal bevel gingivectomy was performed, and the histopathological report was suggestive of telangiectatic granuloma. The intrabony defect was managed with the placement of platelet rich fibrin plug in the defect. A follow-up at 6 months showed no recurrence and no loss in the width of keratinized tissue. The aim of this case is to highlight the rare association of intrabony defect with telangiectatic granuloma and the need for histopathological diagnosis in such lesions.

Research paper thumbnail of Evaluation of potassium binoxalate gel and Nd:YAG laser in the management of dentinal hypersensitivity: a split-mouth clinical and ESEM study

Lasers in Medical Science, 2012

Dentinal hypersensitivity is one of the oldest recorded complaints of discomfort to mankind and y... more Dentinal hypersensitivity is one of the oldest recorded complaints of discomfort to mankind and yet there appears to be no permanent treatment for this clinical condition. This study was designed to evaluate the clinical efficacy of potassium binoxalate gel and neodymium:yttrium-aluminum-garnet (Nd:YAG) laser on dentin hypersensitivity for a period of 9 months. Eighty teeth (20 subjects, 25-55 years old, M = F) were evaluated in a split-mouth design to receive potassium binoxalate (group A, 40 teeth) and Nd:YAG (group B, 40 teeth: 1 W, 10 Hz, and 60 s, irradiated twice). The diameter of output beam was about 300 μm with a distance of 2 mm between laser fiber or tip and tooth surface. The clinical efficacy was evaluated by air-blast test and cold-water test using visual analog scale. Electron microscopy photomicrographs were taken to confirm the results. Analysis was done at baseline; immediately post-treatment; and at 3, 6, 9 months post-treatment. Student&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s paired and unpaired T tests were used to evaluate the statistical analysis. Both treatment modalities were effective in reducing dentine hypersensitivity. However, Nd:YAG laser was better when intragroup comparison was made at 9 months post-treatment. Nd:YAG lasers is better in long-term treatment (up to 9 months) owing to the melting of dentinal tubules. However, due to depth of penetration of microcrystals, gel was better when ease of the procedure is considered. Nevertheless, both treatment modalities resulted in recurrence. Hence, further studies are needed to discover an agent, which can be considered as a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;gold standard&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;.

Research paper thumbnail of Effect of Non-Surgical Periodontal Therapy on Superoxide Dismutase Levels in Gingival Tissues of Chronic Periodontitis Patients: A Clinical and Spectophotometric Analysis

Disease Markers, 2013

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical,... more BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O2.-), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O2.-radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients.METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24–55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gi...

Research paper thumbnail of Zucchelli's technique or tunnel technique with subepithelial connective tissue graft for treatment of multiple gingival recessions

Journal of the International Academy of Periodontology, 2014

Gingival recession is both unpleasant and unesthetic. Meeting the esthetic and functional demands... more Gingival recession is both unpleasant and unesthetic. Meeting the esthetic and functional demands of patients with multiple gingival recessions remains a major therapeutic challenge. We compared the clinical effectiveness of Zucchelli's technique and tunnel technique with subepithelial connective tissue graft (SECTG) for multiple gingival recessions. Twenty systemically and periodontally healthy subjects having 75 recession defects (Miller's class I or II, 39 test and 36 control sites) were included. After initial nonsurgical therapy, test sites were treated with Zucchelli's technique and control sites with tunnel technique with SECTG. Plaque index, bleeding index, pocket depth, recession depth, clinical attachment level, and keratinized gingiva height were evaluated at baseline, 3 and 6 months post-surgery. The mean root coverage was 89.33% +/- 14.47% and 80.00% +/- 15.39% in the test and control groups respectively, with no significant difference between groups. Statis...

Research paper thumbnail of Comparative Evaluation of Efficacy of Antibiotics incorporated Platelet Rich Fibrin Versus Platelet Rich Fibrin alone in the Treatment of Intrabony Defects

Journal of Population Therapeutics and Clinical Pharmacology

Research paper thumbnail of Effect of non-surgical periodontal therapy on superoxide dismutase levels in gingival tissues of chronic periodontitis patients: a clinical and spectophotometric analysis

DOAJ (DOAJ: Directory of Open Access Journals), 2013

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical,... more BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O .− 2), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O .− 2 radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients. METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24-55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gingival index, bleeding index, plaque index. SOD activities were assessed spectrophotometrically at baseline and 2 months post NSPT, results were analysed statistically. RESULTS: At baseline, patients with chronic periodontitis had higher mean SOD activity (2.73 ± 1.36) than the control subjects (1.12 ± 1.13) with p = 0.00003 (p < 0.05). At 2 months post NSPT median SOD level (1.00) had come close to median SOD value of control group (0.85); p = 0.99 (p > 0.05). The resolution of inflammation with successful NSPT resulted in decreased SOD levels as in control group. Clinical parameters in patients with chronic periodontitis showed a significant improvement 2 months post NSPT (p < 0.05). CONCLUSION: Non-surgical periodontal therapy significantly improves the clinical parameters and restores previously increased SOD levels to normal in chronic periodontitis patients.

Research paper thumbnail of Comparative evaluation of clinical efficacy of systemically administered commercially available antioxidant formulations as an adjunct to scaling and root planing in the treatment of chronic periodontitis

Asian Journal of Pharmaceutical Research and Health Care, 2023

Research paper thumbnail of Comparative Evaluation of Coronally Advanced Flap with and without Bioactive Glass Putty in the Management of Gingival Recession Defects: A Randomized Controlled Clinical Trial

PubMed, Jan 14, 2016

Background: The need-of-the-hour is a material that can support coronally advanced flap (CAF) pro... more Background: The need-of-the-hour is a material that can support coronally advanced flap (CAF) procedures in treatment of gingival recession. Recent literature shows that various bone substitutes are being used for this procedure. This study clinically evaluates the outcomes of CAF with and without bioactive glass putty (NovaBone®) in terms of root coverage, gains in keratinized tissue height, and root coverage esthetic score in multiple gingival recession defects. Methods: Ten healthy patients (age range 18-45 years) with multiple bilateral (n = 40 test 20; control 20) and comparable Miller's Class I or Class II gingival recession defects were selected. The defects were randomly assigned by a computer-generated list to either test (CAF + bioactive glass putty) or control (CAF alone) groups. Clinical parameters included gingival recession (GR), probing pocket depth (PPD), clinical attachment level (CAL), keratinized tissue height (KTH), root coverage esthetic score (RES) evaluated at baseline and at 6 months post-surgery CAF with or without bioactive glass putty. Results: Six months post-surgery all clinical parameters showed significant reductions. Gingival recession showed significant reduction both in test and control groups (2.0 ± 0.47 mm and 2.3 ± 0.48 mm, respectively; p < 0.05) with no intergroup difference. The exposed root was covered by 72% (test) and 79% (control). CAL gain was also significant in both groups (test: 2.7 ± 0.67 mm; control: 2.8 ± 0.78 mm; p < 0.05) with no intergroup difference. Keratinized tissue height gain was significant in both the groups (test group: 1.2 ± 0.42 mm; control group: 0.9 ± 0.57 mm) with no intergroup difference. Also, the RES was significant for both the test and control groups (7.2 ± 2.78 and 7.7 ± 1.41 respectively) with no intergroup differences. Conclusions: In isolated Class I/II GR defects, CAF associated with bioactive glass putty provided no significant difference in root coverage, CAL, KTH or RES compared to CAF alone. However, statistically significant gains were seen in all the parameters in both groups as compared to baseline. We refute the claims of the recent studies using a bone substitute for root coverage. Further long-term clinical trials are warranted to substantiate our results.

Research paper thumbnail of Effect of Non-Surgical Periodontal Therapy on Superoxide Dismutase Levels in Gingival Tissues of Chronic Periodontitis Patients: A Clinical and Spectophotometric Analysis

Disease Markers, 2013

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical,... more BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O .− 2), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O .− 2 radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients. METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24-55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gingival index, bleeding index, plaque index. SOD activities were assessed spectrophotometrically at baseline and 2 months post NSPT, results were analysed statistically. RESULTS: At baseline, patients with chronic periodontitis had higher mean SOD activity (2.73 ± 1.36) than the control subjects (1.12 ± 1.13) with p = 0.00003 (p < 0.05). At 2 months post NSPT median SOD level (1.00) had come close to median SOD value of control group (0.85); p = 0.99 (p > 0.05). The resolution of inflammation with successful NSPT resulted in decreased SOD levels as in control group. Clinical parameters in patients with chronic periodontitis showed a significant improvement 2 months post NSPT (p < 0.05). CONCLUSION: Non-surgical periodontal therapy significantly improves the clinical parameters and restores previously increased SOD levels to normal in chronic periodontitis patients.

Research paper thumbnail of Comparative evaluation of platelet-rich fibrin biomaterial and open flap debridement in the treatment of two and three wall intrabony defects

PubMed, Apr 1, 2015

Background: Platelet-rich concentrates are the most widely used regenerative biomaterials. Stimul... more Background: Platelet-rich concentrates are the most widely used regenerative biomaterials. Stimulation and acceleration of soft and hard tissue healing are due to local and continuous delivery of growth factors and proteins, mimicking the needs of the physiological wound healing and reparative tissue processes. This article aims to evaluate the clinical efficacy of open flap debridement (OFD) with or without platelet-rich fibrin (PRF) in the treatment of intrabony defects. Materials and methods: Twenty subjects with forty intrabony defects were treated with either autologous PRF with open-flap debridement (test, n = 20) or open-flap debridement alone (control, n = 20). Soft tissue parameters included: Plaque index, sulcus bleeding index, probing depth, relative attachment level and gingival marginal level (GML). The hard tissue parameters included-distances from: Cement enamel junction to the base of the defect (CEJ-BOD): Alveolar crest to the base of the defect (AC-BOD): And CEJ to AC. The parameters were recorded at baseline and at 9 months postoperatively calculated using standardized radiographs by image-analysis software. Results: Statistically significant (0.005*) intragroup improvements were seen with all the hard and soft parameters in both test and control groups, except for GML. Statistically significant improvements were seen with the mean defect fill (CEJ-BOD and AC-BOD) (P = 0.003*) when intergroup comparisons were made. Conclusions: Adjunctive use of PRF with OFD significantly improves defect fill when compared to OFD alone. PRF has consistently been showing regenerative potential; it is simple, easy and inexpensive biomaterial compared with bone grafts.

Research paper thumbnail of Comparative evaluation of clinical efficacy of systemically administered commercially available antioxidant formulations as an adjunct to scaling and root planing in the treatment of chronic periodontitis

Asian Journal of Pharmaceutical Research and Health Care

Research paper thumbnail of Efficacy of One Stage Full Mouth Disinfection on the Oral Health-Related Quality of Life in Patients with Generalised Chronic Periodontitis

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH

Introduction: Intraoral translocation of periodontal pathogens from untreated intraoral niches ca... more Introduction: Intraoral translocation of periodontal pathogens from untreated intraoral niches can lead to recolonisation and reinfection of recently treated periodontal pockets. Poor oral health hampers the patient’s Quality of Life (QoL) as it causes social isolation, inferiority and the development of psychiatric conditions. Full Mouth Disinfection (FMD) performed within 24 hours using chlorhexidine has reported significant improvements in clinical, microbiological and psychological parameters when compared to conventional periodontal treatment. Aim: To investigate the long-term effect of FMD on Oral HealthRelated Quality of Life (OHRQoL) in patients with generalised chronic periodontitis. Materials and Methods: This study was a prospective clinical study conducted in the Department of Periodontology, Dr DY Patil Dental College and Hospital, Pune, Maharashtra, India, from October 2019 to December 2021. Study consisted of 60 participants who were allotted into two groups; 30 perio...

Research paper thumbnail of Evaluation of Efficacy of Locally Delivered 10% Azadirachta indica (Neem) Gel as an Adjunct to Scaling and Root Planing in the Treatment of Chronic Periodontitis Patients with Type II Diabetes Mellitus: Clinico-Microbiological Study

International Journal of Medical Research and Health Sciences, 2021

Research paper thumbnail of Efficacy of natural tooth pontic in periodontally compromised patients: A systematic review

Journal of Dental Research and Review, 2021

Periodontitis is a major concern for the dentist because the destruction of periodontal fibers ca... more Periodontitis is a major concern for the dentist because the destruction of periodontal fibers causes mobility of teeth and eventually leads to tooth loss. Conventional techniques like removable temporary acrylic prosthesis, or resin-bonded bridges hold their own disadvantages and a fixed prosthesis may worsen the existing condition. Natural tooth pontic (NTP) in such cases may be advantageous in terms of esthetics and patient satisfaction along with cost effectiveness. Hence, the aim of this review was to evaluate the efficacy of NTP in periodontally compromised patients. Electronic search on data sources such as PubMed and Google scholar along with hand searching of the articles was done in institutional library. All case reports and case series were included in English language published till October 31, 2019, were included. After applying inclusion and exclusion criteria, ten articles were selected for the present review. The whole process was conducted by following the Preferre...

Research paper thumbnail of Unmasking Oral Malodor : A Review

Oral malodor is one of the major growing concern of patients today as it is fundamental to their ... more Oral malodor is one of the major growing concern of patients today as it is fundamental to their overall personality. Although the majority of malodor is of oral origin, there are multiple other systemic causes that have to be addressed. Correct diagnosis of the cause of the malodor and prompt treatment can render the patient satisfied. There have been newer developments in faster and more efficient detection of the levels of malodor, and an array of treatment options are present in the market today. It is crucial for the dentist to have a sound understanding of this prevalent oral condition and provide effective intervention programs. This article reviews the various causes and the diagnostic modalities which will help us treat this multifaceted condition.

Research paper thumbnail of Estimation of Beta-CTXin gingival crevicular fluid and serum of periodontally healthy individuals and chronic periodontitis patients before and after non-surgical periodontal therapy- An in-vivo study

IP International Journal of Periodontology and Implantology, 2019

Objective and Design: Beta Cross-linked C-terminal telopeptide of type I collagen (fragment β- CT... more Objective and Design: Beta Cross-linked C-terminal telopeptide of type I collagen (fragment β- CTX) is newer addition to the list of bone resorption markers in serum. The purpose of our study was to estimate and compare the levels β-CTX in gingival crevicular fluid (GCF) and serum of periodontally healthy individuals and chronic periodontitis (CP) patients before and after nonsurgical periodontal therapy (NSPT). Materials and Methods: Twenty Five subjects with equal distribution of males and females were divided into two groups i.e Group I: 10 Periodontally healthy individuals, Group II: 15 CP patients aged between 30-50 years. GCF and serum samples were collected at baseline in Group I whereas in Group II GCF and serum samples were collected at baseline and 2 months after NSPT. The levels of GCF and serum β-CTX were quantified using enzyme-linked immunosorbent assay (ELISA). The clinical parameters evaluated were plaque index (PI), gingival index (GI), probing pocket depth (PPD) and clinical attachment level (CAL) and the correlations of β-CTX in GCF and serum with clinical parameters were evaluated. Results: The mean concentration of β-CTX increased from Group I to Group II. The mean concentration of β-CTX in GCF and serum was reduced after 2 months of NSPT. Furthermore, reduction in β-CTX levels was positively correlated to gain in CAL in GCF. Conclusion: Periodontal treatment resulted in the reduction of β-CTX levels suggesting that β- CTX can be considered as a potential marker of bone resorption in CP.

Research paper thumbnail of Management of internal and external resorption with open apex

Journal of Interdisciplinary Dentistry, 2019

Tooth resorption is a phenomenon resulting in the loss of tooth structure. A simultaneous occurre... more Tooth resorption is a phenomenon resulting in the loss of tooth structure. A simultaneous occurrence of internal resorption and communicating external resorption is often challenging for a clinician. To elicit immediate root canal treatment is of utmost importance, followed by the repair of defect to achieve promising results. For successful outcome, thorough investigations and obtaining a three-dimensional view of the extent and location of resorption should be obtained in order to plan the treatment.

Research paper thumbnail of Efficacy of free gingival graft in the treatment of Miller Class I and Class II localized gingival recessions: A systematic review

Journal of Indian Society of Periodontology, 2019

Background: Currently, the leading theme in mucogingival surgery is the correction of gingival re... more Background: Currently, the leading theme in mucogingival surgery is the correction of gingival recession defects. Free gingival graft (FGG) has been successfully in use in this category of reconstructive therapeutic modality. Objectives: The aim of this systematic review was to evaluate the literature with respect to efficacy of FGG in the management of Miller Class I and II localized gingival recessions. Data Sources: Search strategies were performed via electronic database which included Pubmed-Medline, Google scholar and manual search using University library resources. Two reviewers assessed the eligibility of the studies. Study Eligibility Criteria: Controlled clinical trials, randomized clinical trials and longitudinal studies evaluating recession areas treated by FGG with minimum of 6 months follow up were included. In-vitro and animal studies, studies mainly done on Miller Class III and IV gingival recession defect, studies on multiple gingival recessions and case series and case reports were excluded from the search. Results: The electronic and manual search identified a total of 557 articles. A final screen consisted of 39 articles out of which 17 articles were selected for full-text assessment. Finally, 7 articles were selected for detailed evaluation for this systematic review. FGG has shown significant results in all the studies except for one study. Conclusion: FGG produces substantial results, however, highly depends on the case selection and operator's skill and experience. FGG gives an impression of being the best alternative option in zones where gingival recession presents with inadequate width of attached gingiva and depth of vestibular fornix.

Research paper thumbnail of Sub‐epithelial connective tissue graft for the management of Miller's class I and class II isolated gingival recession defect: A systematic review of the factors influencing the outcome

Journal of Investigative and Clinical Dentistry, 2018

The aim of the present systematic review was to analyze the factors that affect the outcome of su... more The aim of the present systematic review was to analyze the factors that affect the outcome of subepithelial connective tissue graft (SCTG) for managing Miller's class I and class II isolated gingival recession defect. The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses) guidelines for systematic reviews were used. Quality assessments of selected articles were performed. Data on root surface condition, recession type defect, flap thickness, different flap designs, different harvesting techniques, presence/absence of the epithelial collar, graft thickness, flap tension, suturing techniques, and smoking‐related outcomes on root coverage were assessed. The SCTG procedure provides the best root coverage outcomes for Miller's class I and class II recession. The critical threshold of flap thickness was found to be 1 mm. Maximum root coverage was achieved by envelope and modified tunnel technique. SCTG with the epithelial collar does not provide additiona...

Research paper thumbnail of Comparative Study of Measurement Variability using An Electronic Probe and A Manual Probe – “An Eye Opener” - An In Vitro Study

Journal of the Indian Dental Association, Feb 28, 2012

A periodontal probe is considered as an eye below the gingival sulcus. It is widely used to measu... more A periodontal probe is considered as an eye below the gingival sulcus. It is widely used to measure both the probing depth and the attachment loss in order to assess periodontal destruction. However, the use of the periodontal probe particularly in estimating probing depths is subject to large errors. The dimension of the probe tip, the force applied, angle of insertion and root contour all influence the depth recorded. Probing is highly subjected to inter and intra examiner variability and variations in probing force is evident between different examiners. The aim of this study was to compare the relative inter and intra examiner reproducibility of two different periodontal probes – Electronic (florida probe) and the Manual (UNC – 15 probe). Duplicate pocket depth recordings were obtained by three examiners of a single incisor, premolar and molar for each subject at 6 sites. Teeth were equally distributed between the arches and incorporated both shallow (getter than 5mm) and deeper sites (lesser than 5mm) as measured with manual probe. Measurements were repeated 1 week later to avoid bias due to examiner memory.

Research paper thumbnail of Oral telangiectatic granuloma with an intrabony defect

Journal of Indian Society of Periodontology, 2015

Oral telangiectatic granuloma is a benign hyperplastic lesion occurring in response to trauma or ... more Oral telangiectatic granuloma is a benign hyperplastic lesion occurring in response to trauma or chronic irritation in the oral cavity. The characteristic histological appearance comprises of typical granulation tissue with a proliferation of small thin-walled blood vessels in the loose connective tissue. We describe a case of a 36-year-old female who had a swelling in the left maxillary region which was associated with the intrabony defect. An internal bevel gingivectomy was performed, and the histopathological report was suggestive of telangiectatic granuloma. The intrabony defect was managed with the placement of platelet rich fibrin plug in the defect. A follow-up at 6 months showed no recurrence and no loss in the width of keratinized tissue. The aim of this case is to highlight the rare association of intrabony defect with telangiectatic granuloma and the need for histopathological diagnosis in such lesions.

Research paper thumbnail of Evaluation of potassium binoxalate gel and Nd:YAG laser in the management of dentinal hypersensitivity: a split-mouth clinical and ESEM study

Lasers in Medical Science, 2012

Dentinal hypersensitivity is one of the oldest recorded complaints of discomfort to mankind and y... more Dentinal hypersensitivity is one of the oldest recorded complaints of discomfort to mankind and yet there appears to be no permanent treatment for this clinical condition. This study was designed to evaluate the clinical efficacy of potassium binoxalate gel and neodymium:yttrium-aluminum-garnet (Nd:YAG) laser on dentin hypersensitivity for a period of 9 months. Eighty teeth (20 subjects, 25-55 years old, M = F) were evaluated in a split-mouth design to receive potassium binoxalate (group A, 40 teeth) and Nd:YAG (group B, 40 teeth: 1 W, 10 Hz, and 60 s, irradiated twice). The diameter of output beam was about 300 μm with a distance of 2 mm between laser fiber or tip and tooth surface. The clinical efficacy was evaluated by air-blast test and cold-water test using visual analog scale. Electron microscopy photomicrographs were taken to confirm the results. Analysis was done at baseline; immediately post-treatment; and at 3, 6, 9 months post-treatment. Student&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s paired and unpaired T tests were used to evaluate the statistical analysis. Both treatment modalities were effective in reducing dentine hypersensitivity. However, Nd:YAG laser was better when intragroup comparison was made at 9 months post-treatment. Nd:YAG lasers is better in long-term treatment (up to 9 months) owing to the melting of dentinal tubules. However, due to depth of penetration of microcrystals, gel was better when ease of the procedure is considered. Nevertheless, both treatment modalities resulted in recurrence. Hence, further studies are needed to discover an agent, which can be considered as a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;gold standard&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;.

Research paper thumbnail of Effect of Non-Surgical Periodontal Therapy on Superoxide Dismutase Levels in Gingival Tissues of Chronic Periodontitis Patients: A Clinical and Spectophotometric Analysis

Disease Markers, 2013

BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical,... more BACKGROUND: Superoxide dismutase (SOD), an antioxidant acting against superoxide (oxygen radical, O2.-), it is released in inflammatory pathways and causes connective tissue breakdown. Increased SOD activity in inflamed gingiva may indicate increased O2.-radical generation by neutrophils and other inflammatory cells at the diseased site. The aim of the study was to evaluate the effects of non-surgical periodontal therapy (NSPT) on SOD levels in gingival tissues of chronic periodontitis patients.METHODS: Forty subjects: 20 periodontally healthy (Control) and 20 chronic periodontitis (Test); age range 24–55 years were recruited. Gingival tissue samples were collected by excising the inner lining of the periodontal pocket at baseline (prior to non-surgical periodontal therapy) and 2 months post therapy. In controls, tissue samples were obtained immediately after tooth extraction scheduled for orthodontic reasons. Clinical parameters included probing depth, clinical attachment level, gi...

Research paper thumbnail of Zucchelli's technique or tunnel technique with subepithelial connective tissue graft for treatment of multiple gingival recessions

Journal of the International Academy of Periodontology, 2014

Gingival recession is both unpleasant and unesthetic. Meeting the esthetic and functional demands... more Gingival recession is both unpleasant and unesthetic. Meeting the esthetic and functional demands of patients with multiple gingival recessions remains a major therapeutic challenge. We compared the clinical effectiveness of Zucchelli's technique and tunnel technique with subepithelial connective tissue graft (SECTG) for multiple gingival recessions. Twenty systemically and periodontally healthy subjects having 75 recession defects (Miller's class I or II, 39 test and 36 control sites) were included. After initial nonsurgical therapy, test sites were treated with Zucchelli's technique and control sites with tunnel technique with SECTG. Plaque index, bleeding index, pocket depth, recession depth, clinical attachment level, and keratinized gingiva height were evaluated at baseline, 3 and 6 months post-surgery. The mean root coverage was 89.33% +/- 14.47% and 80.00% +/- 15.39% in the test and control groups respectively, with no significant difference between groups. Statis...