Zeeshan Anjum - Academia.edu (original) (raw)

Papers by Zeeshan Anjum

Genetics Research

EDSS1, a syndrome characterized by ectodermal dysplasia-syndactyly, is inherited in an autosomal ... more EDSS1, a syndrome characterized by ectodermal dysplasia-syndactyly, is inherited in an autosomal recessive manner due to mutations in the NECTIN4/PVRL4 gene. Clinical manifestations of the syndrome include defective nail plate, sparse to absent scalp and body hair, spaced teeth with enamel hypoplasia, and bilateral cutaneous syndactyly in the fingers and toes. Here, we report a consanguineous family of Kashmiri origin presenting features of EDSS1. Using whole exome sequencing, we found a recurrent nonsense mutation (NM_030916: c.181C > T, p.(Gln61 ∗ )) in the NECTIN4 gene. The variant segregated perfectly with the disorder within the family. The candidate variant was absent in 50 in-house exomes pertaining to other disorders from the same population. In addition to the previously reported clinical phenotype, an upper lip cleft was found in one of the affected members as a novel phenotype that is not reported by previous studies in EDSS1 patients. Therefore, the study presented he...

Berberis lyceum Royle has a long history of medicinal uses to treat different diseases. Dry fruit... more Berberis lyceum Royle has a long history of medicinal uses to treat different diseases. Dry fruits and roots of this species are medicinally important and are extensively used in many parts of the world. The samples of this species were randomly collected from five districts of Azad Kashmir, Pakistan, including 35 locations. In this study fruits, leaves, stem, roots, and thorn of B. lyceum were used. The morphological studies were conducted to evaluate its qualitative and quantitative traits. For genomic analysis, DNA was extracted from fresh leaves and confirmed on 1 % agarose gel electrophoresis. Fifteen samples were selected for phylogenetic analysis by using four markers including matK, rbcL, ITS, and trnH-psbA. Morphological data showed a difference in their values due to the variations in their altitude, climatic conditions, and soil texture. The phylogenetic study and sequence demarcation tool (SDT) analysis revealed that B. lycium Royle sequences identified in the current st...

Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age... more Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age and obesity. It could be managed by exercise and weight loss. Drugs currently in use like NSAIDs are only for pain relieving and inflammation control and are linked with serious side effects. Surgical options should only be considered when there is no other choice and function is lost. Further studies are needed and new medicines should be discovered for OA treatment.

World academy of science, engineering and technology, 2017

Evidence-Based Complementary and Alternative Medicine, 2021

Berberis lycium Royle has a long history of medicinal uses to treat different diseases. It natura... more Berberis lycium Royle has a long history of medicinal uses to treat different diseases. It naturally grows on the mountains of Indian subcontinent. Its ethnobotanical and biochemical study from the state of Azad Jammu and Kashmir (AJ&K) was not previously explored. So, the objective of the current study was to explore the ethnobotanical and biochemical properties of the B. lycium Royle population of AJ&K. For this purpose, samples of B. lycium Royle were randomly collected from five districts of Azad Jammu and Kashmir, including thirty-five locations. Demographic features of informants such as plant part used, methods of preparation, modes of administration, conservation status, and ethnomedicinal uses were documented. It was used for treating different diseases such as diabetes, arthritis, joint pain, and stomach ulcer. This plant is very famous for providing medicinal roots, leaves, and fruits which are extensively used in many parts of the world. The biochemical analysis was cond...

International Journal of Biosciences (IJB), 2016

Cranberry extract is a famous crude drug used in urinary tract infection all over the world and r... more Cranberry extract is a famous crude drug used in urinary tract infection all over the world and research is being carried out on its different aspects in urinary tract infection treatment. In patient with recurrent urinary tract infection and prophylaxis use of anti-microbial is required for prolong period. It is an effective way but on the cost of adverse effects and increasing resistance among uro-pathogens has pursued the researchers to dig out other alternative anti-microbial agents for treatment of recurrent urinary tract infection. This fact has created interest in Cranberry (Vaccinium macrocarpon) for its use in recurrent urinary tract infection. One hundred patients from two hospitals were studied. Using well diffusion method E. coli resistance against Cranberry was checked in-vitro. E. coli showed resistance against CB extract powder in both centers. Cranberry has no direct effect on E. coli but its effectiveness in urinary tract infection along with antibiotics is due to its anti-motility, anti-adherence effects.

Pakistan Journal of Botany

Assay and drug development technologies, Jan 7, 2018

The presence of P-glycoprotein in the human intestine represents a significant barrier to effecti... more The presence of P-glycoprotein in the human intestine represents a significant barrier to effective drug therapy. These proteins form a multidrug-resistant barrier to most drugs, especially those administered orally. Thus, strategies are needed to prepare molecules to combat these resistant proteins and enable an increase in drug efficacy. We developed a novel tin-Schiff base complex using an ultrasonic bath, a new technique in small molecule synthesis. New bond formation was confirmed using ultraviolet and Fourier transform spectroscopies. A computational study was carried out using Absorption, Distribution, Metabolism, Excretion and Toxicity software. The novel tin-entecavir (ETV)-piperonal Schiff base acts as a potent P-glycoprotein inhibitor, which overcomes the multidrug resistance to all drugs that are substrates for P-glycoprotein. Further study showed that the novel tin complex is less toxic than the parent compound at the same dose. The development of this tin-piperonal-ETV...

Cephalosporin is widely used group prescribed as broad-spectrum antibiotic, containing β-lactam r... more Cephalosporin is widely used group prescribed as broad-spectrum antibiotic, containing β-lactam ring. Numerous different bacteriological pathogens are now resistant against cephalosporin due to over use. Subsequently it is necessary to synthesize new derivatives to enhance biological activity against pathogens. In current study cephalosporin were reacted with essential trace elements to synthesize respective metal complexes. The novel compounds were characterized at different spectroscopic techniques like UV, FTIR, HPLC and AAS (atomic absorption spectroscopy). Biological screening of novel compounds was accomplished against diverse group of pathogens. Some of our novel compounds showed high efficacy against the pathogens as compare to parent composites. We have introduced Novel method for ceftriaxone assays at HPLC using simple and safe mobile phase through metal complexation. Toxicity studies were carried out in vitro and with ADMET software. Novel complexes were found less toxic as compare to parent compounds.

Cranberry extract is a famous crude drug used in urinary tract infection all over the world and r... more Cranberry extract is a famous crude drug used in urinary tract infection all over the world and research is being carried out on its different aspects in urinary tract infection treatment. In patient with recurrent urinary tract infection and prophylaxis use of anti-microbial is required for prolong period. It is an effective way but on the cost of adverse effects and increasing resistance among uro-pathogens has pursued the researchers to dig out other alternative anti-microbial agents for treatment of recurrent urinary tract infection. This fact has created interest in Cranberry (Vaccinium macrocarpon) for its use in recurrent urinary tract infection. One hundred patients from two hospitals were studied. Using well diffusion method E. coli resistance against Cranberry was checked in-vitro. E. coli showed resistance against CB extract powder in both centers. Cranberry has no direct effect on E. coli but its effectiveness in urinary tract infection along with antibiotics is due to its anti-motility, anti-adherence effects.

Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age... more Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age and obesity. It could be managed by exercise and weight loss. Drugs currently in use like NSAIDs are only for pain relieving and inflammation control and are linked with serious side effects. Surgical options should only be considered when there is no other choice and function is lost. Further studies are needed and new medicines should be discovered for OA treatment.

Purpose of study: This study was aimed to make a comprehensive review over prevalence of osteoart... more Purpose of study: This study was aimed to make a comprehensive review over prevalence of osteoarthritis and risk factors associated with it and to relate the latest findings with it. Summary: Osteoarthritis is the major chronic disease causing disability. Patients suffering from it feel pain and become unable to walk or work. Mostly affected joints were hip, knee, hand and foot. Its classification and phenotypes were also discussed. Recent studies also agreed that obesity and aging are the major risks for the prevalence of osteoarthritis. Malnutrition and socioeconomic condition also has impact on disease prevalence along with heredity factors. Occupation with severe workload on joints and injuries increase risk of osteoarthritis. Females are getting this disease more compared to men. Diagnosis methods are progressing and MRI is more helpful in diagnosis. Osteoarthritis showed increase in prevalence during previous years. Person level and joint level risks studies were also reviewed.

Genetics Research

EDSS1, a syndrome characterized by ectodermal dysplasia-syndactyly, is inherited in an autosomal ... more EDSS1, a syndrome characterized by ectodermal dysplasia-syndactyly, is inherited in an autosomal recessive manner due to mutations in the NECTIN4/PVRL4 gene. Clinical manifestations of the syndrome include defective nail plate, sparse to absent scalp and body hair, spaced teeth with enamel hypoplasia, and bilateral cutaneous syndactyly in the fingers and toes. Here, we report a consanguineous family of Kashmiri origin presenting features of EDSS1. Using whole exome sequencing, we found a recurrent nonsense mutation (NM_030916: c.181C > T, p.(Gln61 ∗ )) in the NECTIN4 gene. The variant segregated perfectly with the disorder within the family. The candidate variant was absent in 50 in-house exomes pertaining to other disorders from the same population. In addition to the previously reported clinical phenotype, an upper lip cleft was found in one of the affected members as a novel phenotype that is not reported by previous studies in EDSS1 patients. Therefore, the study presented he...

Berberis lyceum Royle has a long history of medicinal uses to treat different diseases. Dry fruit... more Berberis lyceum Royle has a long history of medicinal uses to treat different diseases. Dry fruits and roots of this species are medicinally important and are extensively used in many parts of the world. The samples of this species were randomly collected from five districts of Azad Kashmir, Pakistan, including 35 locations. In this study fruits, leaves, stem, roots, and thorn of B. lyceum were used. The morphological studies were conducted to evaluate its qualitative and quantitative traits. For genomic analysis, DNA was extracted from fresh leaves and confirmed on 1 % agarose gel electrophoresis. Fifteen samples were selected for phylogenetic analysis by using four markers including matK, rbcL, ITS, and trnH-psbA. Morphological data showed a difference in their values due to the variations in their altitude, climatic conditions, and soil texture. The phylogenetic study and sequence demarcation tool (SDT) analysis revealed that B. lycium Royle sequences identified in the current st...

Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age... more Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age and obesity. It could be managed by exercise and weight loss. Drugs currently in use like NSAIDs are only for pain relieving and inflammation control and are linked with serious side effects. Surgical options should only be considered when there is no other choice and function is lost. Further studies are needed and new medicines should be discovered for OA treatment.

World academy of science, engineering and technology, 2017

Evidence-Based Complementary and Alternative Medicine, 2021

Berberis lycium Royle has a long history of medicinal uses to treat different diseases. It natura... more Berberis lycium Royle has a long history of medicinal uses to treat different diseases. It naturally grows on the mountains of Indian subcontinent. Its ethnobotanical and biochemical study from the state of Azad Jammu and Kashmir (AJ&K) was not previously explored. So, the objective of the current study was to explore the ethnobotanical and biochemical properties of the B. lycium Royle population of AJ&K. For this purpose, samples of B. lycium Royle were randomly collected from five districts of Azad Jammu and Kashmir, including thirty-five locations. Demographic features of informants such as plant part used, methods of preparation, modes of administration, conservation status, and ethnomedicinal uses were documented. It was used for treating different diseases such as diabetes, arthritis, joint pain, and stomach ulcer. This plant is very famous for providing medicinal roots, leaves, and fruits which are extensively used in many parts of the world. The biochemical analysis was cond...

International Journal of Biosciences (IJB), 2016

Cranberry extract is a famous crude drug used in urinary tract infection all over the world and r... more Cranberry extract is a famous crude drug used in urinary tract infection all over the world and research is being carried out on its different aspects in urinary tract infection treatment. In patient with recurrent urinary tract infection and prophylaxis use of anti-microbial is required for prolong period. It is an effective way but on the cost of adverse effects and increasing resistance among uro-pathogens has pursued the researchers to dig out other alternative anti-microbial agents for treatment of recurrent urinary tract infection. This fact has created interest in Cranberry (Vaccinium macrocarpon) for its use in recurrent urinary tract infection. One hundred patients from two hospitals were studied. Using well diffusion method E. coli resistance against Cranberry was checked in-vitro. E. coli showed resistance against CB extract powder in both centers. Cranberry has no direct effect on E. coli but its effectiveness in urinary tract infection along with antibiotics is due to its anti-motility, anti-adherence effects.

Pakistan Journal of Botany

Assay and drug development technologies, Jan 7, 2018

The presence of P-glycoprotein in the human intestine represents a significant barrier to effecti... more The presence of P-glycoprotein in the human intestine represents a significant barrier to effective drug therapy. These proteins form a multidrug-resistant barrier to most drugs, especially those administered orally. Thus, strategies are needed to prepare molecules to combat these resistant proteins and enable an increase in drug efficacy. We developed a novel tin-Schiff base complex using an ultrasonic bath, a new technique in small molecule synthesis. New bond formation was confirmed using ultraviolet and Fourier transform spectroscopies. A computational study was carried out using Absorption, Distribution, Metabolism, Excretion and Toxicity software. The novel tin-entecavir (ETV)-piperonal Schiff base acts as a potent P-glycoprotein inhibitor, which overcomes the multidrug resistance to all drugs that are substrates for P-glycoprotein. Further study showed that the novel tin complex is less toxic than the parent compound at the same dose. The development of this tin-piperonal-ETV...

Cephalosporin is widely used group prescribed as broad-spectrum antibiotic, containing β-lactam r... more Cephalosporin is widely used group prescribed as broad-spectrum antibiotic, containing β-lactam ring. Numerous different bacteriological pathogens are now resistant against cephalosporin due to over use. Subsequently it is necessary to synthesize new derivatives to enhance biological activity against pathogens. In current study cephalosporin were reacted with essential trace elements to synthesize respective metal complexes. The novel compounds were characterized at different spectroscopic techniques like UV, FTIR, HPLC and AAS (atomic absorption spectroscopy). Biological screening of novel compounds was accomplished against diverse group of pathogens. Some of our novel compounds showed high efficacy against the pathogens as compare to parent composites. We have introduced Novel method for ceftriaxone assays at HPLC using simple and safe mobile phase through metal complexation. Toxicity studies were carried out in vitro and with ADMET software. Novel complexes were found less toxic as compare to parent compounds.

Cranberry extract is a famous crude drug used in urinary tract infection all over the world and r... more Cranberry extract is a famous crude drug used in urinary tract infection all over the world and research is being carried out on its different aspects in urinary tract infection treatment. In patient with recurrent urinary tract infection and prophylaxis use of anti-microbial is required for prolong period. It is an effective way but on the cost of adverse effects and increasing resistance among uro-pathogens has pursued the researchers to dig out other alternative anti-microbial agents for treatment of recurrent urinary tract infection. This fact has created interest in Cranberry (Vaccinium macrocarpon) for its use in recurrent urinary tract infection. One hundred patients from two hospitals were studied. Using well diffusion method E. coli resistance against Cranberry was checked in-vitro. E. coli showed resistance against CB extract powder in both centers. Cranberry has no direct effect on E. coli but its effectiveness in urinary tract infection along with antibiotics is due to its anti-motility, anti-adherence effects.

Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age... more Osteoarthritis (OA) is degenerative chronic disease of joints which is common with increasing age and obesity. It could be managed by exercise and weight loss. Drugs currently in use like NSAIDs are only for pain relieving and inflammation control and are linked with serious side effects. Surgical options should only be considered when there is no other choice and function is lost. Further studies are needed and new medicines should be discovered for OA treatment.

Purpose of study: This study was aimed to make a comprehensive review over prevalence of osteoart... more Purpose of study: This study was aimed to make a comprehensive review over prevalence of osteoarthritis and risk factors associated with it and to relate the latest findings with it. Summary: Osteoarthritis is the major chronic disease causing disability. Patients suffering from it feel pain and become unable to walk or work. Mostly affected joints were hip, knee, hand and foot. Its classification and phenotypes were also discussed. Recent studies also agreed that obesity and aging are the major risks for the prevalence of osteoarthritis. Malnutrition and socioeconomic condition also has impact on disease prevalence along with heredity factors. Occupation with severe workload on joints and injuries increase risk of osteoarthritis. Females are getting this disease more compared to men. Diagnosis methods are progressing and MRI is more helpful in diagnosis. Osteoarthritis showed increase in prevalence during previous years. Person level and joint level risks studies were also reviewed.