Anna Pozzi - Academia.edu (original) (raw)

Papers by Anna Pozzi

The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be i... more The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be important on the pression selection finalized to a meat of certificated quality because produce carcasses of considerable value ranking in the highest categories of the EUROP-classification thanks to the meat potential and the low fat rates. For these characteristics the beef cattles with these mutations have spread out widely all over the world. The Real-Time PCR technique is here proposed as a rapid genotyping tool of three known mutations of myostatin gene in three beef cattle breeds

Microorganisms

This study aimed at examining and comparing the nutrient removal efficiency, biomass productivity... more This study aimed at examining and comparing the nutrient removal efficiency, biomass productivity and microbial community structure of two outdoor pilot-scale photobioreactors, namely a bubble column and a raceway pond, treating the liquid fraction of an agricultural digestate. Bacterial and eukaryotic communities were characterized using a metabarcoding approach and quantitative PCR. The abundance, composition, diversity, and dynamics of the main microbes were then correlated to the environmental conditions and operational parameters of the reactors. Both photobioreactors were dominated either by Chlorella sp. or Scenedesmus sp. in function of temperature, irradiance and the nitrogen compounds derived by nitrification. Other species, such as Chlamydomonas and Planktochlorella, were sporadically present, demonstrating that they have more specific niche requirement. Pseudomonas sp. always dominated the bacterial community in both reactors, except in summertime, when a bloom of Caloth...

Mammalian Biology Zeitschrift Fur Saugetierkunde, 1997

Conservation Genetics Resources, 2011

... Mahnaz Shabani • Mohsen Mardi • Alimorad Sarafrazi • Sepehr Mohajeri Naraghi • Heshmatollah R... more ... Mahnaz Shabani • Mohsen Mardi • Alimorad Sarafrazi • Sepehr Mohajeri Naraghi • Heshmatollah Rahimian • Mahmoud Shojaee • Mehrshad Zeinolabedini ... Subtropical Plant Sci J 59:64–67 DietrichCH, Rakitov RA, Holmes JH, Black IV (2001) Phylogeny of the major lineages ...

Gilthead seabream (Sparus aurata) represents one of the most important species for aquaculture in... more Gilthead seabream (Sparus aurata) represents one of the most important species for aquaculture in the Mediterranean region and its interest in selective breeding programs is increasing, given the evidence of large potential gains for selective improvement of economical traits. DNA molecular markers are especially useful in selective breeding programmes for establishing pedigrees without having to raise families separately. In this work twenty-two microsatellite markers were isolated from a complementary DNA (cDNA) library of gilthead seabream. Fourteen microsatellites were selected for designing primers, with 12 of these giving polymorphic loci. Thirty-two individuals randomly chosen from an hatchery broodstock population were characterized, revealing a number of alleles ranging from 4 to 23 and a mean expected and observed heterozygosity varying from 0.37 to 0.93 and from 0.34 to 0.94, respectively. Inheritance and linkage were assessed using different know families. A PCR-multiple...

Single nucleotide polymorphisms (SNPs) have recently been proposed as an alternative to microsate... more Single nucleotide polymorphisms (SNPs) have recently been proposed as an alternative to microsatellites for genotyping individuals. SNPs occur frequently in the genome and, respect to other polymorphisms commonly used in genetic analysis, can be assayed with high-throughput technologies. The aim of this study was to characterize 31 SNPs markers that we have previously detected in gilthead seabream, which may be useful for natural populations studies and in aquaculture selective breeding programs. A DNA pool of 10 different individuals was used to estimated the allele frequencies by sequence chromatogram peaks ratio. To confirm the correct estimation of the allele frequency, the DNA sequences of the pool were individually genotyped using TaqMan probes in real time PCR. The levels of polymorphism of the SNPs were established in 94 unrelated individuals. Among the tested markers, 27 resulted polymorphic, with 18 of these showing allele frequencies between 0,3 and 0,7. The probability o...

Animal genetics, 2014

The objectives of the present work were to verify whether simultaneous exposure to Hoechst 33342 ... more The objectives of the present work were to verify whether simultaneous exposure to Hoechst 33342 and UV irradiation during sorting by flow cytometry may induce gene point mutations in bovine sperm and to assess whether the dye incorporated in the sperm may imply a mutagenic effect during the embryonic development. To this aim, high-resolution melt analysis (HRMA) was used to discriminate variations of single nucleotides in sexed vs. non-sexed control samples. Three batches of sorted and non-sorted commercial semen of seven bulls (42 samples) were subjected to HRMA. A set of 139 genes located on all the chromosomes was selected, and 407 regions of the genome covering a total of 83 907 bases were analyzed. Thereafter, sperm of one sexed and one non-sexed batch of each bull was used in in vitro fertilization, and the derived embryos were analyzed (n = 560). One hundred and thirty-three regions of the bovine genome, located in 40 genes, were screened for a total coverage of 23 397 bases...

Fuel Cells Bulletin, 2013

Asian Pacific Journal of Reproduction, 2014

and Research (MIUR) as a part of the FISR (DM 17/12/02), Project "Tecniche di clonazione bovina e... more and Research (MIUR) as a part of the FISR (DM 17/12/02), Project "Tecniche di clonazione bovina e sicurezza alimentare del latte (TECLA)". (3.4-03 ± 2.4-03 amoles/ 毺 L) and 80 control (2.1-03 ± 1.8-03 amoles/ 毺 L) embryos. Conclusions: The results have revealed a moderate shortening of telomeres in the offspring of the clone with respect to control. However, this study did not evidence differences in the two progenies that suggest welfare problems during the first year of life.

… di Zootecnia Meat Science & Research, 2004

The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be i... more The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be important on the pression selection finalized to a meat of certificated quality because produce carcasses of considerable value ranking in the highest categories of the EUROP-classification thanks to the meat potential and the low fat rates. For these characteristics the beef cattles with these mutations have spread out widely all over the world. The Real-Time PCR technique is here proposed as a rapid genotyping tool of three known mutations of myostatin gene in three beef cattle breeds

animal, 2009

Despite many recent advances in single nucleotide polymorphism (SNP) genotyping technologies, the... more Despite many recent advances in single nucleotide polymorphism (SNP) genotyping technologies, there is still the need for methodologies with a reasonable throughput. In this study, we compared three PCR-based methods for SNP detection: (1) a conventional PCR-based allele detection system with fluorescent genotyping technology, (2) a SNaPshot methodology by single nucleotide primer extension and, (3) a real-time PCR-based method by allele-specific minor groove-binder probes. These three methodologies were used to analyze 104 meat samples of a particular Italian cattle breed known for producing excellent quality meat and for a characteristic increased development of muscle mass, caused by a point mutation (C313Y) in theMyostatingene. The analysis revealed 98 samples to be homozygous (mh/mh) and five to be heterozygous (mh/+) for the mutation whereas one sample resulted to be homozygous for the wild type (+/+). The results obtained with the three different assays were consistent. Overa...

Animal Reproduction Science, 2012

To date, no single in vitro assessment can estimate bull fertility. This research was aimed at ev... more To date, no single in vitro assessment can estimate bull fertility. This research was aimed at evaluating the ability of a series of laboratory assessments to assign 50 Holstein Friesian bulls grouped as low (ER-NRR<-1.5), medium (-0.5<ER-NRR<+0.5) and high (ER-NRR≥+1.5) fertility based on estimated relative non-return rates (ER-NRR), to the two categories of low and medium-high fertility. Heterospermic insemination with a Piedmontese reference bull was employed to define an index of competitive binding ability (CBI) to the zona pellucida using fluorochrome-labeled sperm, and a competitive fertility index (CFI) using embryo paternal assignment by single nucleotide polymorphism (SNP). Furthermore, kinetic parameters, membrane integrity and sperm DNA/chromatin integrity (% DFI) were assayed. Low fertility bulls had lesser (P<0.05) values for total motility and membrane integrity, and a greater value of % DFI as compared to medium and high fertility groups. A modest (P<0.001) correlation was reported among ER-NRR and total motility (r=0.30), progressive motility (r=0.26), membrane integrity (r=0.43) and % DFI (r=-0.26). While % DFI alone allowed the identification of 70% lowly fertile bulls, combining membrane integrity, average path velocity and CBI allowed for identification of 78% of the lowly fertile sires. Paternal assignment by SNPs resulted in 96% of successful assignments and could provide an alternative support to microsatellites for in vivo studies based on heterospermic fertilization for estimating fertility.

African Journal of Microbiology Research, 2012

The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be i... more The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be important on the pression selection finalized to a meat of certificated quality because produce carcasses of considerable value ranking in the highest categories of the EUROP-classification thanks to the meat potential and the low fat rates. For these characteristics the beef cattles with these mutations have spread out widely all over the world. The Real-Time PCR technique is here proposed as a rapid genotyping tool of three known mutations of myostatin gene in three beef cattle breeds

Microorganisms

This study aimed at examining and comparing the nutrient removal efficiency, biomass productivity... more This study aimed at examining and comparing the nutrient removal efficiency, biomass productivity and microbial community structure of two outdoor pilot-scale photobioreactors, namely a bubble column and a raceway pond, treating the liquid fraction of an agricultural digestate. Bacterial and eukaryotic communities were characterized using a metabarcoding approach and quantitative PCR. The abundance, composition, diversity, and dynamics of the main microbes were then correlated to the environmental conditions and operational parameters of the reactors. Both photobioreactors were dominated either by Chlorella sp. or Scenedesmus sp. in function of temperature, irradiance and the nitrogen compounds derived by nitrification. Other species, such as Chlamydomonas and Planktochlorella, were sporadically present, demonstrating that they have more specific niche requirement. Pseudomonas sp. always dominated the bacterial community in both reactors, except in summertime, when a bloom of Caloth...

Mammalian Biology Zeitschrift Fur Saugetierkunde, 1997

Conservation Genetics Resources, 2011

... Mahnaz Shabani • Mohsen Mardi • Alimorad Sarafrazi • Sepehr Mohajeri Naraghi • Heshmatollah R... more ... Mahnaz Shabani • Mohsen Mardi • Alimorad Sarafrazi • Sepehr Mohajeri Naraghi • Heshmatollah Rahimian • Mahmoud Shojaee • Mehrshad Zeinolabedini ... Subtropical Plant Sci J 59:64–67 DietrichCH, Rakitov RA, Holmes JH, Black IV (2001) Phylogeny of the major lineages ...

Gilthead seabream (Sparus aurata) represents one of the most important species for aquaculture in... more Gilthead seabream (Sparus aurata) represents one of the most important species for aquaculture in the Mediterranean region and its interest in selective breeding programs is increasing, given the evidence of large potential gains for selective improvement of economical traits. DNA molecular markers are especially useful in selective breeding programmes for establishing pedigrees without having to raise families separately. In this work twenty-two microsatellite markers were isolated from a complementary DNA (cDNA) library of gilthead seabream. Fourteen microsatellites were selected for designing primers, with 12 of these giving polymorphic loci. Thirty-two individuals randomly chosen from an hatchery broodstock population were characterized, revealing a number of alleles ranging from 4 to 23 and a mean expected and observed heterozygosity varying from 0.37 to 0.93 and from 0.34 to 0.94, respectively. Inheritance and linkage were assessed using different know families. A PCR-multiple...

Single nucleotide polymorphisms (SNPs) have recently been proposed as an alternative to microsate... more Single nucleotide polymorphisms (SNPs) have recently been proposed as an alternative to microsatellites for genotyping individuals. SNPs occur frequently in the genome and, respect to other polymorphisms commonly used in genetic analysis, can be assayed with high-throughput technologies. The aim of this study was to characterize 31 SNPs markers that we have previously detected in gilthead seabream, which may be useful for natural populations studies and in aquaculture selective breeding programs. A DNA pool of 10 different individuals was used to estimated the allele frequencies by sequence chromatogram peaks ratio. To confirm the correct estimation of the allele frequency, the DNA sequences of the pool were individually genotyped using TaqMan probes in real time PCR. The levels of polymorphism of the SNPs were established in 94 unrelated individuals. Among the tested markers, 27 resulted polymorphic, with 18 of these showing allele frequencies between 0,3 and 0,7. The probability o...

Animal genetics, 2014

The objectives of the present work were to verify whether simultaneous exposure to Hoechst 33342 ... more The objectives of the present work were to verify whether simultaneous exposure to Hoechst 33342 and UV irradiation during sorting by flow cytometry may induce gene point mutations in bovine sperm and to assess whether the dye incorporated in the sperm may imply a mutagenic effect during the embryonic development. To this aim, high-resolution melt analysis (HRMA) was used to discriminate variations of single nucleotides in sexed vs. non-sexed control samples. Three batches of sorted and non-sorted commercial semen of seven bulls (42 samples) were subjected to HRMA. A set of 139 genes located on all the chromosomes was selected, and 407 regions of the genome covering a total of 83 907 bases were analyzed. Thereafter, sperm of one sexed and one non-sexed batch of each bull was used in in vitro fertilization, and the derived embryos were analyzed (n = 560). One hundred and thirty-three regions of the bovine genome, located in 40 genes, were screened for a total coverage of 23 397 bases...

Fuel Cells Bulletin, 2013

Asian Pacific Journal of Reproduction, 2014

and Research (MIUR) as a part of the FISR (DM 17/12/02), Project "Tecniche di clonazione bovina e... more and Research (MIUR) as a part of the FISR (DM 17/12/02), Project "Tecniche di clonazione bovina e sicurezza alimentare del latte (TECLA)". (3.4-03 ± 2.4-03 amoles/ 毺 L) and 80 control (2.1-03 ± 1.8-03 amoles/ 毺 L) embryos. Conclusions: The results have revealed a moderate shortening of telomeres in the offspring of the clone with respect to control. However, this study did not evidence differences in the two progenies that suggest welfare problems during the first year of life.

… di Zootecnia Meat Science & Research, 2004

The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be i... more The myostatin gene codes for the double muscling phenotype. Polymorphisms in this gene could be important on the pression selection finalized to a meat of certificated quality because produce carcasses of considerable value ranking in the highest categories of the EUROP-classification thanks to the meat potential and the low fat rates. For these characteristics the beef cattles with these mutations have spread out widely all over the world. The Real-Time PCR technique is here proposed as a rapid genotyping tool of three known mutations of myostatin gene in three beef cattle breeds

animal, 2009

Despite many recent advances in single nucleotide polymorphism (SNP) genotyping technologies, the... more Despite many recent advances in single nucleotide polymorphism (SNP) genotyping technologies, there is still the need for methodologies with a reasonable throughput. In this study, we compared three PCR-based methods for SNP detection: (1) a conventional PCR-based allele detection system with fluorescent genotyping technology, (2) a SNaPshot methodology by single nucleotide primer extension and, (3) a real-time PCR-based method by allele-specific minor groove-binder probes. These three methodologies were used to analyze 104 meat samples of a particular Italian cattle breed known for producing excellent quality meat and for a characteristic increased development of muscle mass, caused by a point mutation (C313Y) in theMyostatingene. The analysis revealed 98 samples to be homozygous (mh/mh) and five to be heterozygous (mh/+) for the mutation whereas one sample resulted to be homozygous for the wild type (+/+). The results obtained with the three different assays were consistent. Overa...

Animal Reproduction Science, 2012

To date, no single in vitro assessment can estimate bull fertility. This research was aimed at ev... more To date, no single in vitro assessment can estimate bull fertility. This research was aimed at evaluating the ability of a series of laboratory assessments to assign 50 Holstein Friesian bulls grouped as low (ER-NRR<-1.5), medium (-0.5<ER-NRR<+0.5) and high (ER-NRR≥+1.5) fertility based on estimated relative non-return rates (ER-NRR), to the two categories of low and medium-high fertility. Heterospermic insemination with a Piedmontese reference bull was employed to define an index of competitive binding ability (CBI) to the zona pellucida using fluorochrome-labeled sperm, and a competitive fertility index (CFI) using embryo paternal assignment by single nucleotide polymorphism (SNP). Furthermore, kinetic parameters, membrane integrity and sperm DNA/chromatin integrity (% DFI) were assayed. Low fertility bulls had lesser (P<0.05) values for total motility and membrane integrity, and a greater value of % DFI as compared to medium and high fertility groups. A modest (P<0.001) correlation was reported among ER-NRR and total motility (r=0.30), progressive motility (r=0.26), membrane integrity (r=0.43) and % DFI (r=-0.26). While % DFI alone allowed the identification of 70% lowly fertile bulls, combining membrane integrity, average path velocity and CBI allowed for identification of 78% of the lowly fertile sires. Paternal assignment by SNPs resulted in 96% of successful assignments and could provide an alternative support to microsatellites for in vivo studies based on heterospermic fertilization for estimating fertility.

African Journal of Microbiology Research, 2012