Anna Sawilska - Academia.edu (original) (raw)
Papers by Anna Sawilska
Progress in Plant Protection, 2010
Springer eBooks, 2019
The aim of the study has been the phytoaccumulation of trace elements in the plants of evening pr... more The aim of the study has been the phytoaccumulation of trace elements in the plants of evening primrose Oenothera biennis L. from the areas of Kuyavia-Pomerania province. The soil and plants were sampled from natural stands (the edges of woodlands) from selected locations in Kuyavia-Pomerania province: A – Łosiny (53°44′29.1″N; 17°55′45.9″E), B – Okole (53°07′50″N; 17°58′24″E), C – Rynarzewo (53°3′55.42″N; 17°49′3.25″E), D – Łochowo (53°07′19″N; 17°50′19″E). The plants were collected in July 2017 at flowering and fruit development stages. In dried and mineralized plant material and in soil samples the total content of Zn, Cu, Mn, Fe, Pb, Cd and Ni was assayed with the Atomic Absorption Spectrometry (AAS) with spectrometer PHILIPS PU 9100X. The content of carbon was assayed with analyser Vario Max CN, made by Elementar. The soils were not polluted with heavy metals. The content of Pb, Cd in the evening primrose organs did not exceed the admissible pollution norms. The lowest amount of Pb (<0.2 mg kg−1 d.m.) did not exceed the admissible pollution norms. The lowest amount of Pb (<0.2 mg kg−1 d.m.) was found in seeds. The plants from stands C, D accumulated considerable amounts of Mn. The content of that element in stems and leaves ranged from 45.1 mg kg−1 in d.m. in stand A to 575 mg kg−1 of d.m. in stand D, whereas in seeds – on average from 18.9 mg kg−1 of d.m. in stand A to 265.5 mg kg−1 of d.m. in stand D.
Zeszyty Naukowe Akademii Techniczno-Rolniczej w Bydgoszczy. Rolnictwo, 1995
Electronic Journal of Polish Agricultural Universities. Series Agronomy, 1998
Acta Societatis Botanicorum Poloniae, 2010
Herba Polonica, 2009
S ummary During the years 2007–2008 the content of flavonoids and polyphenolic acids in infloresc... more S ummary During the years 2007–2008 the content of flavonoids and polyphenolic acids in inflorescences of Helichrysum arenarium (L.) Moench was analyzed in specimens from natural stands and plantations. Plants cultivated in experimental plots originated from in ...
Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial p... more Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial protection in Poland whose inflorescences are a source of valuable herbal material. Field observations were conducted over five consecutive growth seasons at permanent study plots in the Bory Tucholskie forest complex, Poland. The plots were in secondary anthropogenic replacement communities, which develop on poor sandy soils. Phytocenoses of initial, medium and most advanced succession were distinguished by the species composition of the communities. The aim of this study was to determine the density and morphological structure of shoots and ramets of the sandy everlastings, and of their potential and actual fertility as the early phase of secondary succession of swards advanced on sandy soils. Many features showed large variations caused by weather, but the stage of successional advancement in the phytocenoses had the greatest effect on the number of sandy everlasting shoots, their mass and morphology. The estimated yield of herbal material from 1 m2 of a phytocenosis was on average smallest from the plot with the most advanced secondary succession, about half the yield from each of the other two plots. The yield from natural sites varied widely over the years.
Progress in Plant Protection, 2009
Progress in Plant Protection, 2010
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2015
Tissue culture was developed at the beginning of the twentieth century (1907) as a method for stu... more Tissue culture was developed at the beginning of the twentieth century (1907) as a method for studying the behaviour of human and animal cells free of systemic variations that might arise in vivo both during normal homeostasis and under the stress. The technique was established first with disaggregated fragments of tissue, and growth was restricted to the radial migration of cells from the tissue fragment, with occasional mitoses in the outgrowth. As culture of cells from and within such primary explants of tissue dominated the field for more than 50 years, it is not surprising that the name "tissue culture" has remained in use as a generic term despite the fact that most of the expansion in the field in the second half of the twentieth century was made possible by the use of dispersed cell cultures. Disaggregation of explanted cells and subsequent plating out of the dispersed cells was first demonstrated by Rous (Rous and Jones, 1916). In 1950s trypsin became more generally used for subculture, following procedures described by Dulbecco to obtain passaged monolayer cultures for viral plaque assays (Dulbecco, 1952), and the generation of a single cell suspension by trypsinization. Tissue culture enter its second century since its inception. For the first time it is possible for genetic manipulation of commonly and easily cultured cells, such as skin fibroblasts, to allow their conversion into pluripotent stem cells (iPS), capable of differentiating into a range of different cell types. The field opens up to a whole new scenario: instead of the need for complex selective culture technique, the culture procedures may be used to initiate biochemical regulation and may be used to convert phenotype. Tissue culturing nowadays is essential and irreplaceable in modern biotechnology as a method supplementing and enriching our knowledge. Without specialized laboratories for tissue culture with equipment for they analysis and chance of cytogenetic and molecular analysis it will not be possible to by us transgenic rabbits producing in mammary gland human growth hormone and five lines of transgenic pigs for xenotransplantation purposes. In our laboratories we use cell culture of human and animal cells for biotechnology needs or as models. In animal transgenesis we usually perform several steps using molecular and cytogenetic methods at cell culture levels. Important issues in producing transgenic animals cover characteristics of donor of gene, transfection and cell transformation, detection of transgene, mapping of transgene, passing of transgene into next generation, homozygote selection and analysis of transgene activity. From cytogenetic methods the most important are banding chromosomes methods for karyotyping each transgenic individual and mapping the transgene on metaphase chromosomes using fluorescent in situ hybridization (FISH). It is possible to study structure of the transgene on the chromatin level using FISH on chromosomal loop domain or using 3D FISH technique. This methods is important to distinguish homozygous offspring from heterozygous and confirm the stability of transgenesis. The last step include analysis of expression of transgene using flow cytometry methods.
Infrastructure and Environment, 2019
Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial p... more Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial protection in Poland whose inflorescences are a source of valuable herbal material. Field observations were conducted over five consecutive growth seasons at permanent study plots in the Bory Tucholskie forest complex, Poland. The plots were in secondary anthropogenic replacement communities, which develop on poor sandy soils. Phytocenoses of initial, medium and most advanced succession were distinguished by the species composition of the communities. The aim of this study was to determine the density and morphological structure of shoots and ramets of the sandy everlastings, and of their potential and actual fertility as the early phase of secondary succession of swards advanced on sandy soils. Many features showed large variations caused by weather, but the stage of successional advancement in the phytocenoses had the greatest effect on the number of sandy everlasting shoots, their mass...
Progress in Plant Protection, 2009
The plant material were plants of sandy everlasting (Helichrysum arenarium (L.) Moench) collected... more The plant material were plants of sandy everlasting (Helichrysum arenarium (L.) Moench) collected from natural locations in October 2012. Initial explants were apical buds enfolded into two leaves. Isolated explants were chemically sterilized. Reproducted shoots were divided and put into three types of mediums: medium MS (control) containing 37 mg P∙dm-3, medium MS without additive of P (0,0 mg P∙dm-3), medium MS with additional amound of P (74 mg P∙dm-3). The aim of research was to specify the influence of phosphorus content in medium for choosen growth parameters and accumulation of assimilatory pigments: chlorophyll a, b, chlorophyll a+b, carotenoids. Analysis of these compounds were done spectrophotometrically. The made research, proved, that the richest in mentioned substances were microseedlings growing on the medium with increased amount of phosphorus. Phosphorus deficiency in medium MS had a statistically essential effect on changes in growth modifications of microseedlings ...
Progress in Plant Protection, 2010
Springer eBooks, 2019
The aim of the study has been the phytoaccumulation of trace elements in the plants of evening pr... more The aim of the study has been the phytoaccumulation of trace elements in the plants of evening primrose Oenothera biennis L. from the areas of Kuyavia-Pomerania province. The soil and plants were sampled from natural stands (the edges of woodlands) from selected locations in Kuyavia-Pomerania province: A – Łosiny (53°44′29.1″N; 17°55′45.9″E), B – Okole (53°07′50″N; 17°58′24″E), C – Rynarzewo (53°3′55.42″N; 17°49′3.25″E), D – Łochowo (53°07′19″N; 17°50′19″E). The plants were collected in July 2017 at flowering and fruit development stages. In dried and mineralized plant material and in soil samples the total content of Zn, Cu, Mn, Fe, Pb, Cd and Ni was assayed with the Atomic Absorption Spectrometry (AAS) with spectrometer PHILIPS PU 9100X. The content of carbon was assayed with analyser Vario Max CN, made by Elementar. The soils were not polluted with heavy metals. The content of Pb, Cd in the evening primrose organs did not exceed the admissible pollution norms. The lowest amount of Pb (<0.2 mg kg−1 d.m.) did not exceed the admissible pollution norms. The lowest amount of Pb (<0.2 mg kg−1 d.m.) was found in seeds. The plants from stands C, D accumulated considerable amounts of Mn. The content of that element in stems and leaves ranged from 45.1 mg kg−1 in d.m. in stand A to 575 mg kg−1 of d.m. in stand D, whereas in seeds – on average from 18.9 mg kg−1 of d.m. in stand A to 265.5 mg kg−1 of d.m. in stand D.
Zeszyty Naukowe Akademii Techniczno-Rolniczej w Bydgoszczy. Rolnictwo, 1995
Electronic Journal of Polish Agricultural Universities. Series Agronomy, 1998
Acta Societatis Botanicorum Poloniae, 2010
Herba Polonica, 2009
S ummary During the years 2007–2008 the content of flavonoids and polyphenolic acids in infloresc... more S ummary During the years 2007–2008 the content of flavonoids and polyphenolic acids in inflorescences of Helichrysum arenarium (L.) Moench was analyzed in specimens from natural stands and plantations. Plants cultivated in experimental plots originated from in ...
Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial p... more Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial protection in Poland whose inflorescences are a source of valuable herbal material. Field observations were conducted over five consecutive growth seasons at permanent study plots in the Bory Tucholskie forest complex, Poland. The plots were in secondary anthropogenic replacement communities, which develop on poor sandy soils. Phytocenoses of initial, medium and most advanced succession were distinguished by the species composition of the communities. The aim of this study was to determine the density and morphological structure of shoots and ramets of the sandy everlastings, and of their potential and actual fertility as the early phase of secondary succession of swards advanced on sandy soils. Many features showed large variations caused by weather, but the stage of successional advancement in the phytocenoses had the greatest effect on the number of sandy everlasting shoots, their mass and morphology. The estimated yield of herbal material from 1 m2 of a phytocenosis was on average smallest from the plot with the most advanced secondary succession, about half the yield from each of the other two plots. The yield from natural sites varied widely over the years.
Progress in Plant Protection, 2009
Progress in Plant Protection, 2010
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2015
Tissue culture was developed at the beginning of the twentieth century (1907) as a method for stu... more Tissue culture was developed at the beginning of the twentieth century (1907) as a method for studying the behaviour of human and animal cells free of systemic variations that might arise in vivo both during normal homeostasis and under the stress. The technique was established first with disaggregated fragments of tissue, and growth was restricted to the radial migration of cells from the tissue fragment, with occasional mitoses in the outgrowth. As culture of cells from and within such primary explants of tissue dominated the field for more than 50 years, it is not surprising that the name "tissue culture" has remained in use as a generic term despite the fact that most of the expansion in the field in the second half of the twentieth century was made possible by the use of dispersed cell cultures. Disaggregation of explanted cells and subsequent plating out of the dispersed cells was first demonstrated by Rous (Rous and Jones, 1916). In 1950s trypsin became more generally used for subculture, following procedures described by Dulbecco to obtain passaged monolayer cultures for viral plaque assays (Dulbecco, 1952), and the generation of a single cell suspension by trypsinization. Tissue culture enter its second century since its inception. For the first time it is possible for genetic manipulation of commonly and easily cultured cells, such as skin fibroblasts, to allow their conversion into pluripotent stem cells (iPS), capable of differentiating into a range of different cell types. The field opens up to a whole new scenario: instead of the need for complex selective culture technique, the culture procedures may be used to initiate biochemical regulation and may be used to convert phenotype. Tissue culturing nowadays is essential and irreplaceable in modern biotechnology as a method supplementing and enriching our knowledge. Without specialized laboratories for tissue culture with equipment for they analysis and chance of cytogenetic and molecular analysis it will not be possible to by us transgenic rabbits producing in mammary gland human growth hormone and five lines of transgenic pigs for xenotransplantation purposes. In our laboratories we use cell culture of human and animal cells for biotechnology needs or as models. In animal transgenesis we usually perform several steps using molecular and cytogenetic methods at cell culture levels. Important issues in producing transgenic animals cover characteristics of donor of gene, transfection and cell transformation, detection of transgene, mapping of transgene, passing of transgene into next generation, homozygote selection and analysis of transgene activity. From cytogenetic methods the most important are banding chromosomes methods for karyotyping each transgenic individual and mapping the transgene on metaphase chromosomes using fluorescent in situ hybridization (FISH). It is possible to study structure of the transgene on the chromatin level using FISH on chromosomal loop domain or using 3D FISH technique. This methods is important to distinguish homozygous offspring from heterozygous and confirm the stability of transgenesis. The last step include analysis of expression of transgene using flow cytometry methods.
Infrastructure and Environment, 2019
Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial p... more Helichrysum arenarium (L.) Moench is a perennial in the Asteraceae family that is under partial protection in Poland whose inflorescences are a source of valuable herbal material. Field observations were conducted over five consecutive growth seasons at permanent study plots in the Bory Tucholskie forest complex, Poland. The plots were in secondary anthropogenic replacement communities, which develop on poor sandy soils. Phytocenoses of initial, medium and most advanced succession were distinguished by the species composition of the communities. The aim of this study was to determine the density and morphological structure of shoots and ramets of the sandy everlastings, and of their potential and actual fertility as the early phase of secondary succession of swards advanced on sandy soils. Many features showed large variations caused by weather, but the stage of successional advancement in the phytocenoses had the greatest effect on the number of sandy everlasting shoots, their mass...
Progress in Plant Protection, 2009
The plant material were plants of sandy everlasting (Helichrysum arenarium (L.) Moench) collected... more The plant material were plants of sandy everlasting (Helichrysum arenarium (L.) Moench) collected from natural locations in October 2012. Initial explants were apical buds enfolded into two leaves. Isolated explants were chemically sterilized. Reproducted shoots were divided and put into three types of mediums: medium MS (control) containing 37 mg P∙dm-3, medium MS without additive of P (0,0 mg P∙dm-3), medium MS with additional amound of P (74 mg P∙dm-3). The aim of research was to specify the influence of phosphorus content in medium for choosen growth parameters and accumulation of assimilatory pigments: chlorophyll a, b, chlorophyll a+b, carotenoids. Analysis of these compounds were done spectrophotometrically. The made research, proved, that the richest in mentioned substances were microseedlings growing on the medium with increased amount of phosphorus. Phosphorus deficiency in medium MS had a statistically essential effect on changes in growth modifications of microseedlings ...